scholarly journals Efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai pengganti kiserit pada bibit kakao The effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as kieserite substitute on cocoa seedling

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Acid Soils ◽  
Dry Mass ◽  
Test Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Npk Fertilizers ◽  
Standard Dosage ◽  
Tropical Regions ◽  
Mg Deficiency ◽  
Phosphate Solubilizing

Abstract Magnesium (Mg) deficiency is commonly found in acid soils with high weathering intensity as widely present in tropical and sub-tropical regions, or those developed from low Mg-content parent materials. To overcome Mg deficiency, dolomite is often used to replace kieserite although the former is less reactive than the later. An activation technology by heating up to 800oC, followed by sulfic acid addition, then enriched by phosphate solubilizing bacteria is prospective to improve Mg solubility in soils. This research was aimed to enhance dolomite reactivity and to determine the effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as Mg nutrient sources substituting kieserite on cocoa seedling growth. The results indicate that total and solubility in water of MgO from the prototype of activated dolomite were 26.7 and 9.2%, respectively. Using cocoa seedling as test plant, the use of activated dolomite enriched by using 109 cfu of Pseudomonas sp. at 3.85 g/seedling and combined with 100% dosages of NPK fertilizers showed the best vegetative growth and total dry mass of seedlings was significantly higher than that of 100% NPK-Mg kieserite standard dosage Abstrak Defisiensi Mg umumnya ditemukan pada tanah masam dengan tingkat pelapukan tinggi yang banyak terdapat di daerah tropik, subtropik, dan tanah yang terbentuk dari bahan induk miskin Mg. Untuk mengatasi defisiensi Mg, dolomit sering digunakan sebagai pengganti kiserit walaupun sifatnya kurang reaktif jika dibandingkan kiserit. Teknologi aktivasi dengan pemanasan mencapai 800oC, dilanjutkan dengan penambahan asam sulfat serta pengkayaan dengan bakteri pelarut fosfat diharapkan dapat meningkatkan kelarutan Mg di dalam tanah. Penelitian ini bertujuan untuk meningkatkan reaktivitas dolomit serta menetapkan efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai sumber hara Mg pengganti kieserit pada bibit kakao. Hasil yang diperoleh dari penelitian ini mengindikasikan bahwa kadar MgO total dan kelarutan dalam air pada dolomit teraktivasi masing-masing 26,7 dan 9,2%. Dengan menggunakan bibit kakao sebagai tanaman uji, aplikasi 3,85 g dolomit teraktivasi yang diperkaya dengan 109 cfu Pseudomonas sp./bibit yang dikombinasikan dengan 100% dosis pupuk NPK menunjukkan pertumbuhan vegetatif paling baik dan total bobot kering bibit kakao yang secara nyata lebih tinggi jika dibandingkan dengan pemberian 100% dosis standar NPK-Mg kiserit. 

scholarly journals Efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai pengganti kiserit pada bibit kakao The effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as kieserite substitute on cocoa seedling

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Acid Soils ◽  
Dry Mass ◽  
Test Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Npk Fertilizers ◽  
Standard Dosage ◽  
Tropical Regions ◽  
Mg Deficiency ◽  
Phosphate Solubilizing

Abstract Magnesium (Mg) deficiency is commonly found in acid soils with high weathering intensity as widely present in tropical and sub-tropical regions, or those developed from low Mg-content parent materials. To overcome Mg deficiency, dolomite is often used to replace kieserite although the former is less reactive than the later. An activation technology by heating up to 800oC, followed by sulfic acid addition, then enriched by phosphate solubilizing bacteria is prospective to improve Mg solubility in soils. This research was aimed to enhance dolomite reactivity and to determine the effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as Mg nutrient sources substituting kieserite on cocoa seedling growth. The results indicate that total and solubility in water of MgO from the prototype of activated dolomite were 26.7 and 9.2%, respectively. Using cocoa seedling as test plant, the use of activated dolomite enriched by using 109 cfu of Pseudomonas sp. at 3.85 g/seedling and combined with 100% dosages of NPK fertilizers showed the best vegetative growth and total dry mass of seedlings was significantly higher than that of 100% NPK-Mg kieserite standard dosage Abstrak Defisiensi Mg umumnya ditemukan pada tanah masam dengan tingkat pelapukan tinggi yang banyak terdapat di daerah tropik, subtropik, dan tanah yang terbentuk dari bahan induk miskin Mg. Untuk mengatasi defisiensi Mg, dolomit sering digunakan sebagai pengganti kiserit walaupun sifatnya kurang reaktif jika dibandingkan kiserit. Teknologi aktivasi dengan pemanasan mencapai 800oC, dilanjutkan dengan penambahan asam sulfat serta pengkayaan dengan bakteri pelarut fosfat diharapkan dapat meningkatkan kelarutan Mg di dalam tanah. Penelitian ini bertujuan untuk meningkatkan reaktivitas dolomit serta menetapkan efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai sumber hara Mg pengganti kieserit pada bibit kakao. Hasil yang diperoleh dari penelitian ini mengindikasikan bahwa kadar MgO total dan kelarutan dalam air pada dolomit teraktivasi masing-masing 26,7 dan 9,2%. Dengan menggunakan bibit kakao sebagai tanaman uji, aplikasi 3,85 g dolomit teraktivasi yang diperkaya dengan 109 cfu Pseudomonas sp./bibit yang dikombinasikan dengan 100% dosis pupuk NPK menunjukkan pertumbuhan vegetatif paling baik dan total bobot kering bibit kakao yang secara nyata lebih tinggi jika dibandingkan dengan pemberian 100% dosis standar NPK-Mg kiserit. 

scholarly journals Secretion of Gluconic Acid From Nguyenibacter sp. L1 Is Responsible for Solubilization of Aluminum Phosphate

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao Li Li ◽  
Xue Qiang Zhao ◽  
Xiao Ying Dong ◽  
Jian Feng Ma ◽  
Ren Fang Shen
Keyword(s):  
Gluconic Acid ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Aluminum Phosphate ◽  
Available P ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
Major Factors

Phosphorus (P) deficiency is one of the major factors limiting plant growth in acid soils, where most P is fixed by toxic aluminum (Al). Phosphate-solubilizing bacteria (PSBs) are important for the solubilization of fixed P in soils. Many PSBs have been isolated from neutral and calcareous soils, where calcium phosphate is the main P form, whereas PSBs in acid soils have received relatively little attention. In this study, we isolated a PSB strain from the rhizosphere of Lespedeza bicolor, a plant well adapted to acid soils. On the basis of its 16S rRNA gene sequence, this strain was identified as a Nguyenibacter species and named L1. After incubation of Nguyenibacter sp. L1 for 48 h in a culture medium containing AlPO4 as the sole P source, the concentration of available P increased from 10 to 225 mg L–1, and the pH decreased from 5.5 to 2.5. Nguyenibacter sp. L1 exhibited poor FePO4 solubilization ability. When the pH of non-PSB-inoculated medium was manually adjusted from 5.5 to 2.5, the concentration of available P only increased from 6 to 65 mg L–1, which indicates that growth medium acidification was not the main contributor to the solubilization of AlPO4 by Nguyenibacter sp. L1. In the presence of glucose, but not fructose, Nguyenibacter sp. L1 released large amounts of gluconic acid to solubilize AlPO4. Furthermore, external addition of gluconic acid enhanced AlPO4 solubilization and reduced Al toxicity to plants. We conclude that secretion of gluconic acid by Nguyenibacter sp. L1, which is dependent on glucose supply, is responsible for AlPO4 solubilization as well as the alleviation of Al phytotoxicity by this bacterial strain.

scholarly journals Rhizospheric Phosphate Solubilizing Bacillus atrophaeus GQJK17 S8 Increases Quinoa Seedling, Withstands Heavy Metals, and Mitigates Salt Stress

2021 ◽  
Vol 13 (6) ◽  
pp. 3307
Author(s):  
Ismail Mahdi ◽  
Nidal Fahsi ◽  
Mohamed Hafidi ◽  
Saad Benjelloun ◽  
Abdelmounaaim Allaoui ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Germination Rate ◽  
Chenopodium Quinoa ◽  
Hydrocyanic Acid ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Bacillus Atrophaeus ◽  
Genotypic Analysis ◽  
Phosphate Solubilizing ◽  
Enterobacter Asburiae

Introduction of quinoa (Chenopodium quinoa willd.), a gluten-free nutritious pseudo-cereal, outside its traditional growing areas exposed it to seedling damping-off. Here, we isolated eleven phosphate-solubilizing bacteria from the quinoa rhizosphere and assessed their effect on germination and seedlings growth. All isolates solubilized phosphate, produced indole3-acetic acid, hydrocyanic acid, siderophores, and ammonia. Genotypic analysis revealed that our strains are related to the genus of Bacillus, Pseudomonas, and Enterobacter. Strains Enterobacter asburiae (QD14, QE4, QE6, and QE16), Enterobacter sp. QE3, and Enterobacter hormaechei QE7 withstood 1.5 mg·L−1 of cadmium sulfate, 0.5 mg·mL−1 of nickel nitrate, and 1 mg·mL−1 of copper sulfate. Moreover, all strains solubilized zinc from ZnO; P. Stutzeri QD1 and E. asburiae QD14 did not solubilize Zn3(PO4)2 and CO3Zn, whereas CO3Zn was not solubilized by E. asburiae QE16. Bacillus atrophaeus S8 tolerated 11% NaCl. P. frederiksbergensis S6 and Pseudomonas sp. S7 induced biofilm formation. Anti-fusarium activity was demonstrated for E.asburiae QE16, P. stutzeri QD1, P. frederiksbergensis S6, Pseudomonas sp. S7, and B. atrophaeus S8. Lastly, inoculation of quinoa seeds with B. atrophaeus S8 and E. asburiae QB1 induced the best germination rate and seedling growth, suggesting their potential use as inoculants for salty and heavy metal or zinc contaminated soils.

scholarly journals Phosphate Solubilizing Bacteria in Certain Agricultural Crop Soils of Delhi

2018 ◽  
Vol 4 (01) ◽  
pp. 70-75
Author(s):  
Ratul Baishya ◽  
Rhituporna Saikia
Keyword(s):  
Organic Acids ◽  
Solid Medium ◽  
Crop Yields ◽  
Crop Plants ◽  
Agricultural Crop ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Phosphate Solubilizing ◽  
Phosphate Solubilizer ◽  
Halo Zone

Phosphate solubilizing bacteria (PSB) helps in the solubilization of insoluble phosphates and thus lead to increase in crop yields. A study was conducted to isolate and characterize biochemically PSB from different agricultural crop soils of Delhi such as Garlic, Radish, Chilli, Onion and Cabbage. PSB were isolated in Pikovskaya solid medium and formation of solubilization (halo) zone was measured. 16 PSB were isolated and identified. The selected PSB differed in phosphate solubilizing efficiency, production of organic acids and phosphatases. Citrobacter sp. and Pseudomonas sp. were dominant in all the crop plants. Among all the isolates, Pseudomonas sp. proved to be an efficient phosphate solubilizer.

scholarly journals Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera

2020 ◽  
Vol 21 (1) ◽  
pp. 40-48
Author(s):  
Muhammad Asril ◽  
Yuni Lisafitri
Keyword(s):  
Soil Microbes ◽  
Acid Soil ◽  
Acid Soils ◽  
Soil Samples ◽  
Pathogenicity Test ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Potato Tubers ◽  
Phosphate Availability ◽  
Phosphate Solubilizing

ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam 

scholarly journals Microbiological transformations of phosphorus and sulphur compounds in acid soils

2012 ◽  
pp. 27-36
Author(s):  
Dragana Stamenov ◽  
Mirjana Jarak ◽  
Simonida Djuric ◽  
Hajnal Jafari ◽  
Dragana Bjelic
Keyword(s):  
Organic Phosphorus ◽  
Humus Content ◽  
Acid Soils ◽  
Phosphorus Compounds ◽  
Phosphate Solubilizing Bacteria ◽  
Sulphur Compounds ◽  
Organic Sulphur ◽  
Number Of Microorganisms ◽  
Phosphate Solubilizing ◽  
Number Of Bacteria

The dynamics of phosphorus and sulphur in soil is closely related to the dynamics of the biological cycle in which microorganisms play a central role. There is not much microbiological activity in acid soils because aerobes are scarce, rhizosphere is restricted to the shallow surface layer, and the biomass of microorganisms decreases with higher acidity. The aim of the research was to investigate the number of microorganisms, which decompose organic and inorganic phosphorus compounds and organic sulphur compounds in calcocambisol, luvisol, and pseudogley. The following parameters were determined in the soil samples: pH in H2O and in 1MKCl; the content of CaCO3 (%); humus content (%), nitrogen content (%); the content of physiologically active phosphorus and potassium (mg P2O5/100g of soil; mg K2O/100g of soil). The number of microorganisms was determined by the method of agar plates on appropriate nutrient media: the number of microorganisms solubilizing phosphates on a medium by Muramcov; the number of microorganisms that decompose organic phosphorus compounds on a medium with lecithin; and the number of microorganisms that transform organic sulphur compounds on a medium by Baar. All three types of soil are acid non-carbonate soils with a low level of available phosphorus and a more favorable amount of potassium, nitrogen, and humus. The largest number of bacteria, which transform organic phosphorus compounds, was found in calcocambisol. The largest number of phosphate solubilizing bacteria was recorded in pseudogley, whereas the largest number of phosphate solubilizing fungi was recorded in calcocambisol. The largest number of bacteria, which transform organic sulphur compounds, was recorded in pseudogley.

scholarly journals Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

2021 ◽  
Vol 26 (1) ◽  
pp. 79-118
Author(s):  
Andrea Blanco-Vargas ◽  
Lina M Rodríguez-Gacha ◽  
Natalia Sánchez-Castro ◽  
Laura Herrera-Carlosama ◽  
Raúl A. Poutou-Piñales ◽  
...  
Keyword(s):  
Low Cost ◽  
Mineral Fertilizer ◽  
Dry Weight ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Yeast Hydrolysate ◽  
Organic Mineral ◽  
Allium Cepa L ◽  
Essential Nutrient ◽  
Phosphate Solubilizing

Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 g L-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse- and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12 h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5 g L-1) fourfold lower than those in SMRS1 (10.0 g L-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08/ log10 CFUmL-1 and, at 6 h of culture, acid and alkaline phosphatase enzyme volumetric activities of 2.3 ± 0.8 and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17) mg L-1 at 6 h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1) of bioinoculant plus 100 kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables (170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus (p < 0.05).

scholarly journals Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

2019 ◽  
Vol 79 (1) ◽  
pp. 29-37 ◽  
Author(s):  
I. Munir ◽  
A. Bano ◽  
M. Faisal
Keyword(s):  
Growth Parameters ◽  
Phosphate Solubilizing Bacteria ◽  
Acidic Ph ◽  
Pseudomonas Sp ◽  
Percentage Germination ◽  
Pesticide Stress ◽  
Auxin Production ◽  
Rrna Sequencing ◽  
Biochemical Testing ◽  
Phosphate Solubilizing

Abstract Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

Sign in / Sign up

Export Citation Format

Share Document