Analysis of Tegumental Surface Proteins of Schistosoma mansoni Primary Sporocysts

Several methods are being applied for solubilizing adult Schistosoma mansoni antigens which may allow their immunochemical characterization and purification. Studies on antigens and immunoprecipitins in schistosome infections have been carried out with homogenized extracts of adult worms and/or larval forms of Schistosoma mansoni in saline (Biguet et al., 1962; Kagan and Norman, 1963; Silva and Ferri, 1965), or water (Kent, 1963). Kusel (1972) studied surface proteins in S. mansoni membrane, treating the worms with 0·5% saponin in 3% calcium chloride. Murrel et al. (1974) compared by immunodiffusion a crude culture antigen with extracts obtained by freeze-thawing of adult worms and by treatment in 3M KCl.

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Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda)

Parasitology ◽

10.1017/s0031182000065513 ◽

1986 ◽

Vol 92 (3) ◽

pp. 653-664 ◽

Cited By ~ 26

Author(s):

C. J. Bayne ◽

E. S. Loker ◽

Mary A. Yui

Keyword(s):

Schistosoma Mansoni ◽

Plasma Proteins ◽

Biomphalaria Glabrata ◽

Rabbit Antibody ◽

Tegumental Surface ◽

Immunological Interactions ◽

Resistant Strains ◽

Host Parasite ◽

Susceptibility And Resistance

SUMMARYThe tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plasma antigens are present on the surface at near steady state. Though a wide variety of peptides is acquired, there is selection. Furthermore, some differences occur in the peptides acquired from the plasma of susceptible and resistant strains of snail. Acquired antigens are rapidly processed, and are predominantly undetectable in tegumental extracts after a few hours. In contrast, rabbit antibody on sporocysts remains in situ for at least 48 h, so under some conditions there is stable expression of certain tegumental antigenic determinants.These data, obtained using antibodies to snail plasma antigens and to sporocyst tegumental antigens, are discussed in the light of current ideas on the cellular and molecular basis of susceptibility and resistance in this host#parasite system.

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Surface proteins on schistosomula and cercariae of Schistosoma mansoni

International Journal for Parasitology ◽

10.1016/0020-7519(79)90003-1 ◽

1979 ◽

Vol 9 (6) ◽

pp. 491-493 ◽

Cited By ~ 14

Author(s):

R. Ramasamy

Keyword(s):

Schistosoma Mansoni ◽

Surface Proteins

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Potential role of a CD36-like class B scavenger receptor in the binding of modified low-density lipoprotein (acLDL) to the tegumental surface of Schistosoma mansoni sporocysts

Molecular and Biochemical Parasitology ◽

10.1016/j.molbiopara.2005.12.010 ◽

2006 ◽

Vol 146 (2) ◽

pp. 219-230 ◽

Cited By ~ 47

Author(s):

Nathalie Dinguirard ◽

Timothy P. Yoshino

Keyword(s):

Schistosoma Mansoni ◽

Potential Role ◽

Low Density Lipoprotein ◽

Scavenger Receptor ◽

Density Lipoprotein ◽

Low Density ◽

Tegumental Surface ◽

Class B

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In vivo intravascular biotinylation of Schistosoma bovis adult worms and proteomic analysis of tegumental surface proteins

Journal of Proteomics ◽

10.1016/j.jprot.2013.09.020 ◽

2013 ◽

Vol 94 ◽

pp. 513-526 ◽

Cited By ~ 11

Author(s):

Eduardo de la Torre-Escudero ◽

Ricardo Pérez-Sánchez ◽

Raúl Manzano-Román ◽

Ana Oleaga

Keyword(s):

Proteomic Analysis ◽

Surface Proteins ◽

Tegumental Surface ◽

Schistosoma Bovis

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Isolation of cDNAs Encoding Secreted and Transmembrane Proteins from Schistosoma mansoni by a Signal Sequence Trap Method

Infection and Immunity ◽

10.1128/iai.71.5.2548-2554.2003 ◽

2003 ◽

Vol 71 (5) ◽

pp. 2548-2554 ◽

Cited By ~ 50

Author(s):

Danielle Smyth ◽

Donald P. McManus ◽

Michael J. Smout ◽

Thewarach Laha ◽

Wenbao Zhang ◽

...

Keyword(s):

Cell Surface ◽

Schistosoma Mansoni ◽

Developmental Stages ◽

Signal Sequence ◽

Expression Profiles ◽

Surface Proteins ◽

Secreted Proteins ◽

Signal Peptides ◽

Placental Alkaline Phosphatase ◽

Cell Surface Antigens

ABSTRACT Surface and secreted proteins of schistosomes orchestrate the basic physiologic requirements of a parasitic existence. These proteins are often exposed to host tissues during penetration, migration, feeding, and immune evasion, and they are obvious targets for control strategies. Signal sequence trap (SST) represents a novel approach that selects for cDNAs encoding secreted and surface proteins with N-terminal signal peptides, so we constructed a randomly primed adult Schistosoma mansoni cDNA library fused to a signalless reporter gene encoding placental alkaline phosphatase. The library was used to transfect COS-7 cells, which were then assayed for the presence of reporter at the cell surface. Eighteen S. mansoni cDNA fragments were isolated and sequenced. Expression profiles of the novel clones were determined for different developmental stages; some transcripts were restricted to single-sex adult worms, while others were ubiquitously distributed. Most clones contained signal peptides or signal anchors as determined by the SignalP algorithm. Open reading frames (ORFs) were categorized as follows: (i) previously identified S. mansoni cDNAs encoding proteins of known function; (ii) cDNAs encoding proteins of known function in other organisms but novel for Schistosoma; (iii) S. mansoni expressed sequence tags (ESTs) of unknown function; and (iv) completely novel ORFs without homologues (including ESTs) from any phylum. Clones of particular interest included tetraspanins similar to human cell surface antigens, a protein kinase, and ORFs transcribed in the antisense orientation to previously characterized S. mansoni cDNAs. This is the first report describing the use of SST as a tool for identifying secreted proteins from any pathogenic organism.

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