The effect of Tween 80 on the fatty acid composition of Lactobacillusbuchneri and Lactobacillus brevis.

Bile tolerance is a fundamental ability of probiotic bacteria. We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production. The bile tolerance of these strains was significantly lower in modified de Man – Rogosa – Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium. Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media. The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile. In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile. Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains. In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.

Download Full-text

Effects of biotin on glutamate production and fatty acid composition in Bacillus cereus 14B22

Canadian Journal of Microbiology ◽

10.1139/m68-175 ◽

1968 ◽

Vol 14 (10) ◽

pp. 1039-1048 ◽

Cited By ~ 3

Author(s):

Jerry S. Hubbard ◽

Alan N. Hall

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Bacillus Cereus ◽

Acid Composition ◽

Tween 80 ◽

Biotin Deficiency ◽

Branched Chain Fatty Acids ◽

Tween 40 ◽

Branched Chain ◽

High Yields

Bacillus cereus 14B22 exhibits a biochemical regulation by biotin. In glucose–salts or citrate–salts media, limiting amounts of biotin restrict cellular synthesis and induce synthesis of high yields of extracellular L-glutamic acid. With nonlimiting levels of biotin, abundant cellular synthesis occurs without the concomitant accumulation of glutamate. The biotin status of cells was not reflected in experiments with cell-free extracts. Citrate was converted to glutamate at comparable rates by extracts of biotin-sufficient and biotin-deficient cells. The biotin requirement for growth can be replaced with Tween 80 or with a mixture of Tween 40 and oleic acid. The yields of glutamate given by cultures of these "fatty acid-grown cells" were intermediate between those obtained with biotin-deficient and biotin-sufficient cells. Biotin deficiency also altered the fatty acid composition of the cell membrane. The proportion of straight-chain to branched-chain fatty acids is significantly smaller in low-biotin cells than in high-biotin cells. These changes did not affect cellular permeability to glutamate. Variations in the morphology of B. cereus 14B22 as influenced by biotin and the fatty acid composition of the medium are described.

Download Full-text

Effect of Tween 80 on the growth, lipid accumulation and fatty acid composition of Thraustochytrium aureum ATCC 34304

Journal of Bioscience and Bioengineering ◽

10.1016/j.jbiosc.2010.12.010 ◽

2011 ◽

Vol 111 (4) ◽

pp. 420-424 ◽

Cited By ~ 56

Author(s):

Yousuke Taoka ◽

Naoki Nagano ◽

Yuji Okita ◽

Hitoshi Izumida ◽

Shinichi Sugimoto ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Lipid Accumulation ◽

Tween 80 ◽

Thraustochytrium Aureum

Download Full-text

Effect of methyl oleate and Tween 80 on the antibiotic productivity and the fatty acid composition of the total lipids of Streptomyces hygroscopicus CH-7

Journal of the Serbian Chemical Society ◽

10.2298/jsc0008603g ◽

2000 ◽

Vol 65 (8) ◽

pp. 603-607

Author(s):

Gordana Gojgic-Cvijovic ◽

Ivanka Karadzic ◽

Jovan Vucetic

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Methyl Oleate ◽

Acid Composition ◽

Tween 80 ◽

Cellular Fatty Acid ◽

Total Lipids ◽

Main Effect ◽

Essential Nutrient ◽

Branched Chain

The effects of methyl oleate and Tween 80, hexaene H-85 production stimulators, on the fatty acid composition of the total lipids of S. hygroscopicus CH-7 were investigated. Besides oleic acid in the biomass, an increase of the branched chain fatty acid contents was observed: from 1.6 to 2.1 fold in the medium with methyl oleate and 2.5-2.9 in the medium with Tween 80 compared to the base medium. These two additives act differently on the growth of the producing strain and the uptake of valine, an essential nutrient. A decreased proteolytic activity and similar cellular fatty acid profiles indicate that the main effect of the added compounds is to facilitate the excretion of antibiotics to the outer medium, thus increasing biosynthesis.

Download Full-text

Effect of chilling and subsequent storage at 20oC on electrolyte leakage and phospholipid fatty acid composition of tomato pericarp

Physiologia Plantarum ◽

10.1034/j.1399-3054.1993.870412.x ◽

1993 ◽

Vol 87 (4) ◽

pp. 522-527 ◽

Cited By ~ 1

Author(s):

M. Bergevin ◽

G. P. L'Heureux ◽

J. E. Thompson ◽

C. Willemot

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Electrolyte Leakage ◽

Phospholipid Fatty Acid ◽

Phospholipid Fatty Acid Composition ◽

Subsequent Storage

Download Full-text

Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660071 ◽

1985 ◽

Vol 54 (03) ◽

pp. 563-569 ◽

Cited By ~ 19

Author(s):

M K Salo ◽

E Vartiainen ◽

P Puska ◽

T Nikkari

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Platelet Aggregation ◽

Acid Composition ◽

Saturated Fatty Acids ◽

Free Living ◽

Ω3 Fatty Acids ◽

Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

Download Full-text

Health Benefits of Pumpkin Seed and Nutrition Profile of 35 Pumpkin Accessions

EDIS ◽

10.32473/edis-hs1312-2017 ◽

2017 ◽

Vol 2017 (6) ◽

Author(s):

Geoffrey M. Meru ◽

Yuqing Fu ◽

Dayana Leyva ◽

Paul Sarnoski ◽

Yavuz Yagiz

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Health Benefits ◽

Pumpkin Seed ◽

Production Practices

This article aims to summise production and nutrition aspects of pumpkin seed. Specifically, it focuses on health benefits of the seeds, production practices and provides data on the oil, protein and fatty acid composition of 35 pumpkin accessions.

Download Full-text