scholarly journals Effect of methyl oleate and Tween 80 on the antibiotic productivity and the fatty acid composition of the total lipids of Streptomyces hygroscopicus CH-7

2000 ◽  
Vol 65 (8) ◽  
pp. 603-607
Author(s):  
Gordana Gojgic-Cvijovic ◽  
Ivanka Karadzic ◽  
Jovan Vucetic
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Methyl Oleate ◽  
Acid Composition ◽  
Tween 80 ◽  
Cellular Fatty Acid ◽  
Total Lipids ◽  
Main Effect ◽  
Essential Nutrient ◽  
Branched Chain

The effects of methyl oleate and Tween 80, hexaene H-85 production stimulators, on the fatty acid composition of the total lipids of S. hygroscopicus CH-7 were investigated. Besides oleic acid in the biomass, an increase of the branched chain fatty acid contents was observed: from 1.6 to 2.1 fold in the medium with methyl oleate and 2.5-2.9 in the medium with Tween 80 compared to the base medium. These two additives act differently on the growth of the producing strain and the uptake of valine, an essential nutrient. A decreased proteolytic activity and similar cellular fatty acid profiles indicate that the main effect of the added compounds is to facilitate the excretion of antibiotics to the outer medium, thus increasing biosynthesis.

Effects of biotin on glutamate production and fatty acid composition in Bacillus cereus 14B22

1968 ◽  
Vol 14 (10) ◽  
pp. 1039-1048 ◽  
Author(s):  
Jerry S. Hubbard ◽  
Alan N. Hall
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Bacillus Cereus ◽  
Acid Composition ◽  
Tween 80 ◽  
Biotin Deficiency ◽  
Branched Chain Fatty Acids ◽  
Tween 40 ◽  
Branched Chain ◽  
High Yields

Bacillus cereus 14B22 exhibits a biochemical regulation by biotin. In glucose–salts or citrate–salts media, limiting amounts of biotin restrict cellular synthesis and induce synthesis of high yields of extracellular L-glutamic acid. With nonlimiting levels of biotin, abundant cellular synthesis occurs without the concomitant accumulation of glutamate. The biotin status of cells was not reflected in experiments with cell-free extracts. Citrate was converted to glutamate at comparable rates by extracts of biotin-sufficient and biotin-deficient cells. The biotin requirement for growth can be replaced with Tween 80 or with a mixture of Tween 40 and oleic acid. The yields of glutamate given by cultures of these "fatty acid-grown cells" were intermediate between those obtained with biotin-deficient and biotin-sufficient cells. Biotin deficiency also altered the fatty acid composition of the cell membrane. The proportion of straight-chain to branched-chain fatty acids is significantly smaller in low-biotin cells than in high-biotin cells. These changes did not affect cellular permeability to glutamate. Variations in the morphology of B. cereus 14B22 as influenced by biotin and the fatty acid composition of the medium are described.

Cellular fatty acid composition of isolates from Legionnaires disease

1977 ◽  
Vol 6 (2) ◽  
pp. 140-143 ◽  
Author(s):  
C W Moss ◽  
R E Weaver ◽  
S B Dees ◽  
W B Cherry
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Cellular Fatty Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Cellular Fatty Acids ◽  
Legionnaires Disease ◽  
Branched Chain

The cellular fatty acids of four isolates from Legionnaires disease and two antigenically related isolates were identified by gas chromatography, mass spectrometry, and associated techniques. The six isolates had essentially the same fatty acid composition, which was characterized by large amounts (greater than 80%) of branched-chain acids.

scholarly journals Further studies of the cellular fatty acid composition of Legionnaires disease bacteria

1979 ◽  
Vol 9 (5) ◽  
pp. 648-649
Author(s):  
C W Moss ◽  
S B Dees
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Acid Composition ◽  
Cellular Fatty Acid ◽  
Cellular Fatty Acid Composition ◽  
Legionnaires Disease ◽  
Branched Chain

The cellular fatty acid composition of 36 strains of the Legionnaires disease bacterium was determined by gas chromatography after growth on different media. The fatty acid profile of each strain was essentially identical on each medium and was characterized by large amounts (greater than 68%) of branched-chain acids.

Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles

2014 ◽  
Vol 60 (4) ◽  
pp. 183-191 ◽  
Author(s):  
Shigenori Suzuki ◽  
Hiromi Kimoto-Nira ◽  
Hiroyuki Suganuma ◽  
Chise Suzuki ◽  
Tadao Saito ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Sodium Acetate ◽  
Tween 80 ◽  
Lactobacillus Brevis ◽  
Cellular Fatty Acid ◽  
Bile Tolerance ◽  
Cellular Fatty Acid Composition ◽  
Mrs Medium

Bile tolerance is a fundamental ability of probiotic bacteria. We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production. The bile tolerance of these strains was significantly lower in modified de Man – Rogosa – Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium. Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media. The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile. In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile. Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains. In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.

scholarly journals Branched-chain fatty acid composition of human milk and the impact of maternal diet: the Global Exploration of Human Milk (GEHM) Study

2016 ◽  
Vol 105 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Kelly A Dingess ◽  
Christina J Valentine ◽  
Nicholas J Ollberding ◽  
Barbara S Davidson ◽  
Jessica G Woo ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Human Milk ◽  
Acid Composition ◽  
Maternal Diet ◽  
Chain Fatty Acid ◽  
Branched Chain ◽  
The Impact

In Reply: Lipid Composition of Milk From Mothers With Cystic Fibrosis

PEDIATRICS ◽  
1989 ◽  
Vol 83 (4) ◽  
pp. 632-632
Author(s):  
JOEL BITMAN ◽  
MARGIT HAMOSH ◽  
D. L. WOOD ◽  
L. M. FREED ◽  
P. HAMOSH
Keyword(s):  
Cystic Fibrosis ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Lipid Composition ◽  
Total Lipids ◽  
Energy Needs

This report from Golembeski and Emery adds to the small quantity of literature in this field. Drs Golembeski and Emery claim that they are presenting information that counters our statement,"The abnormalities in fatty acid composition of the cystic fibrosis milk may contraindicate its use for the nursing infant." However, no data regarding fatty acid composition were presented. In their Table, only total lipids are shown. In our study, we acknowledged that mean total lipids were sufficient to supply the energy needs of the nursing infant.

Fatty Acid Composition in Fractions of Total Lipids from the Buds of Plants of the Betula L. Genus by the Opening Phases

2021 ◽  
Vol 68 (1) ◽  
pp. 94-101
Author(s):  
I. V. Morozova ◽  
N. P. Chernobrovkina ◽  
M. K. Il’inova ◽  
E. V. Robonen ◽  
V. D. Tsydendambaev ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Total Lipids
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