scholarly journals Hinokitiol acts against Porphyromonas gingivalis by inhibiting Autoinducer-2

2021 ◽  
Vol 63 (4) ◽  
pp. 183-189
Author(s):  
Shoko Hamada ◽  
Mitsuhiro Gomi ◽  
Akihiro Yoshida
Keyword(s):  
Porphyromonas Gingivalis ◽  
Autoinducer 2

scholarly journals LuxS-Based Signaling in Streptococcus gordonii: Autoinducer 2 Controls Carbohydrate Metabolism and Biofilm Formation with Porphyromonas gingivalis

2003 ◽  
Vol 185 (1) ◽  
pp. 274-284 ◽  
Author(s):  
Roderick McNab ◽  
Suzannah K. Ford ◽  
Azza El-Sabaeny ◽  
Bruno Barbieri ◽  
Guy S. Cook ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Surface Expression ◽  
Periodontal Pathogen ◽  
Cell Surface Expression ◽  
Streptococcus Gordonii ◽  
Autoinducer 2 ◽  
Expression Of Genes ◽  
Insertional Inactivation

ABSTRACT Communication based on autoinducer 2 (AI-2) is widespread among gram-negative and gram-positive bacteria, and the AI-2 pathway can control the expression of genes involved in a variety of metabolic pathways and pathogenic mechanisms. In the present study, we identified luxS, a gene responsible for the synthesis of AI-2, in Streptococcus gordonii, a major component of the dental plaque biofilm. S. gordonii conditioned medium induced bioluminescence in an AI-2 reporter strain of Vibrio harveyi. An isogenic mutant of S. gordonii, generated by insertional inactivation of the luxS gene, was unaffected in growth and in its ability to form biofilms on polystyrene surfaces. In contrast, the mutant strain failed to induce bioluminescence in V. harveyi and was unable to form a mixed species biofilm with a LuxS-null strain of the periodontal pathogen Porphyromonas gingivalis. Complementation of the luxS mutation in S. gordonii restored normal biofilm formation with the luxS-deficient P. gingivalis. Differential display PCR demonstrated that the inactivation of S. gordonii luxS downregulated the expression of a number of genes, including gtfG, encoding glucosyltransferase; fruA, encoding extracellular exo-β-d-fructosidase; and lacD encoding tagatose 1,6-diphosphate aldolase. However, S. gordonii cell surface expression of SspA and SspB proteins, previously implicated in mediating adhesion between S. gordonii and P. gingivalis, was unaffected by inactivation of luxS. The results suggest that S. gordonii produces an AI-2-like signaling molecule that regulates aspects of carbohydrate metabolism in the organism. Furthermore, LuxS-dependent intercellular communication is essential for biofilm formation between nongrowing cells of P. gingivalis and S. gordonii.

Distribution of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia in an Australian population

2001 ◽  
Vol 28 (12) ◽  
pp. 1163-1171 ◽  
Author(s):  
S. M. Hamlet ◽  
M. P. Cullinan ◽  
B. Westerman ◽  
M. Lindeman ◽  
P. S. Bird ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Prevotella Intermedia ◽  
Australian Population

Daya Hambat Minyak Hati Ikan Hiu Terhadap Pertumbuhan Bakteri Porphyromonas gingivalis

DENTA ◽  
2015 ◽  
Vol 9 (2) ◽  
pp. 129
Author(s):  
Felisitas Maria Agustina ◽  
Dian Mulawarmanti ◽  
Yoifah Rizka Wedarti
Keyword(s):  
Porphyromonas Gingivalis ◽  
Control Group ◽  
Strain Atcc ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

<p><strong> </strong></p><p><strong>Latar belakang : </strong><em>Porphyromonas gingivalis </em>adalah bakteri paling dominan yang ditemukan pada periodontitis kronis. Antibakteri konvensional(antibiotik), terutama tetrasiklin sering digunakan untuk menunjang terapi periodontal, akan tetapi penggunaan antibiotik sering menimbulkan resisten, alergi, dan toksik.  Minyak hati ikan hiu spesies <em>Cenctrophorus sp </em>memiliki sifat antibakteri melalui kandungannya yaitu <em>squalene </em>dan<em> squalamine, </em>sehingga memiliki potensi untuk dikembangkan sebagai terapi ajuvan pada penyakit periodontal. <strong>Tujuan : </strong>Mengetahui daya hambat minyak hati ikan hiu terhadap pertumbuhan bakteri <em>Porphyromonas gingivalis. </em><strong>Metode : </strong>Penelitian ini menggunakan rancangan penelitian <em>post test only control group design.</em> Subjek penelitian adalah bakteri <em>Porphyromonas gingivalis </em>strain ATCC 33277 sebanyak 30 sampel yang dibagi menjadi 5 kelompok. Tiga kelompok diberi minyak hati ikan hiu dengan konsentrasi 10%, 15%, dan 20%. Kontrol positif diberi tetrasiklin dan kontrol negatif diberi DMSO 1%. Daya hambat diperiksa dengan menggunakan metode difusi dan diinokulasikan pada media <em>MH </em>agar. Daya hambat dihitung dengan mengukur diameter zona jernih pada disk dengan menggunakan <em>digital callipers </em>dalam satuan milimeter. <strong>Hasil : </strong>Minyak hati ikan hiu dapat menghambat pertumbuhan bakteri <em>Porphyromonas gingivalis </em>pada setiap konsentrasi. Konsentrasi 10% (9,06 mm), konsentrasi 15% (11,31 mm), konsentrasi 20% (13,2 mm), sedangkan kontrol negatif (6,01 mm), dan  kontrol positif (30,09 mm). <strong>Simpulan : </strong>Minyak hati ikan hiu dapat menghambat pertumbuhan bakteri <em>Porphyromonas gingivalis.</em><em></em></p><p><em> </em></p>

Биофармацевтические исследования по выбору основы для стоматологического геля с экстрактом барбариса

2020 ◽  
pp. 50-54
Author(s):  
З. Хаджиева ◽  
С. Поройский ◽  
И. Фирсова ◽  
А. Струсовская ◽  
В. Бавлакова ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Prevotella Intermedia ◽  
Prevotella Nigrescens

Исследованы биофармацевтические свойства модельных гелевых композиций c экстрактом корня барбариса и использованием в качестве гелеобразователей метилцеллюлозы, натриевой соли карбоксиметилцеллюлозы, натриевой соли альгиновой кислоты и поливинилового спирта методами прямой диффузии в агаровый гель и диализа через полупроницаемую мембрану. Определены кинетические закономерности процесса и скорости набухания исследуемых гелевых основ. Показана целесообразность использования натриевой соли альгиновой кислоты для получения стоматологического геля с экстрактом корня барбариса, обладающего специфической бактериостатической активностью в отношении таких пародонтопатогенных микроорганизмов, как Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens и Actinobacillus actinomycetemcomitans, для профилактики и лечения воспалительных заболеваний пародонта.

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