scholarly journals Cellular responses of periodontal ligament stem cells to a novel synthesized form of calcium hydrogen phosphate with a hydroxyapatite-like surface for periodontal tissue engineering

2018 ◽  
Vol 60 (3) ◽  
pp. 428-437 ◽  
Author(s):  
Kallapat Tansriratanawong ◽  
Pawinee Wongwan ◽  
Hiroshi Ishikawa ◽  
Taka Nakahara ◽  
Kanet Wongravee
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Periodontal Ligament ◽  
Cellular Responses ◽  
Periodontal Tissue ◽  
Hydrogen Phosphate ◽  
Periodontal Ligament Stem Cells

Application of eGFP to label human periodontal ligament stem cells in periodontal tissue engineering

2012 ◽  
Vol 57 (9) ◽  
pp. 1241-1250 ◽  
Author(s):  
Yong Wen ◽  
Jing Lan ◽  
Haiyun Huang ◽  
Meijiao Yu ◽  
Jun Cui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Periodontal Ligament ◽  
Periodontal Tissue ◽  
Periodontal Ligament Stem Cells

scholarly journals Zein/gelatin/nanohydroxyapatite nanofibrous scaffolds are biocompatible and promote osteogenic differentiation of human periodontal ligament stem cells

2019 ◽  
Vol 7 (5) ◽  
pp. 1973-1983 ◽  
Author(s):  
Qianmin Ou ◽  
Yingling Miao ◽  
Fanqiao Yang ◽  
Xuefeng Lin ◽  
Li-Ming Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cells ◽  
Nanofibrous Scaffolds

In bone tissue engineering, it is important for biomaterials to promote the osteogenic differentiation of stem cells to achieve tissue regeneration.

scholarly journals MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Siqi Yao ◽  
Wei Zhao ◽  
Qianmin Ou ◽  
Lanchen Liang ◽  
Xuefeng Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Target Prediction ◽  
Cell Types ◽  
Luciferase Reporter ◽  
Periodontal Ligament Stem Cells ◽  
Activating Transcription Factor 4 ◽  
Activating Transcription Factor

Periodontitis is the main cause of adult tooth loss. Stem cell-based tissue engineering has become a promising therapy for periodontitis treatment. To date, human periodontal ligament stem cells (hPDLSCs) have been shown to be a favorable source for tissue engineering, but modulatory mechanisms of hPDLSCs remain unclear. Approximately 60% of mammalian genes are the targets of over 2000 miRNAs in multiple human cell types, and miRNAs are able to influence various biological processes in the human body, including bone formation. In this study, we found that after osteogenic induction, miR-214 was significantly decreased in hPDLSCs; therefore, we examined the effects of miR-214 on osteogenic differentiation. Computational miRNA target prediction analyses and luciferase reporter assays revealed that activating transcription factor 4 (ATF4) is a direct target of miR-214. We prepared cells overexpressing miR-214 and found that miR-214 negatively regulates osteogenic differentiation of hPDLSCs. For the target of miR-214, ATF4 protein expression level was decreased after induction. In conclusion, we found that miR-214-ATF4 axis is a novel pathway for regulating hPDLSC osteogenic differentiation.

scholarly journals Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 605 ◽  
Author(s):  
Dorina Lauritano ◽  
Luisa Limongelli ◽  
Giulia Moreo ◽  
Gianfranco Favia ◽  
Francesco Carinci
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Growth Factors ◽  
Bone Regeneration ◽  
Full Text ◽  
Periodontal Ligament ◽  
Cochrane Library ◽  
Periodontal Tissue

Introduction. Several biomaterials are used in periodontal tissue engineering in order to obtain a three-dimensional scaffold, which could enhance the oral bone regeneration. These novel biomaterials, when placed in the affected area, activate a cascade of events, inducing regenerative cellular responses, and replacing the missing tissue. Natural and synthetic polymers can be used alone or in combination with other biomaterials, growth factors, and stem cells. Natural-based polymer chitosan is widely used in periodontal tissue engineering. It presents biodegradability, biocompatibility, and biological renewability properties. It is bacteriostatic and nontoxic and has hemostatic and mucoadhesive capacity. The aim of this systematic review is to obtain an updated overview of the utilization and effectiveness of chitosan-based scaffold (CS-bs) in the alveolar bone regeneration process. Materials and Methods. During database searching (using PubMed, Cochrane Library, and CINAHL), 72 items were found. The title, abstract, and full text of each study were carefully analyzed and only 22 articles were selected. Thirteen articles were excluded based on their title, five after reading the abstract, twenty-six after reading the full text, and six were not considered because of their publication date (prior to 2010). Quality assessment and data extraction were performed in the twelve included randomized controlled trials. Data concerning cell proliferation and viability (CPV), mineralization level (M), and alkaline phosphatase activity (ALPA) were recorded from each article Results. All the included trials tested CS-bs that were combined with other biomaterials (such as hydroxyapatite, alginate, polylactic-co-glycolic acid, polycaprolactone), growth factors (basic fibroblast growth factor, bone morphogenetic protein) and/or stem cells (periodontal ligament stem cells, human jaw bone marrow-derived mesenchymal stem cells). Values about the proliferation of cementoblasts (CB) and periodontal ligament cells (PDLCs), the activity of alkaline phosphatase, and the mineralization level determined by pure chitosan scaffolds resulted in lower than those caused by chitosan-based scaffolds combined with other molecules and biomaterials. Conclusions. A higher periodontal regenerative potential was recorded in the case of CS-based scaffolds combined with other polymeric biomaterials and bioceramics (bio compared to those provided by CS alone. Furthermore, literature demonstrated that the addition of growth factors and stem cells to CS-based scaffolds might improve the biological properties of chitosan.

scholarly journals An antibacterial and injectable calcium phosphate scaffold delivering human periodontal ligament stem cells for bone tissue engineering

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40157-40170
Author(s):  
Hong Chen ◽  
Hui Yang ◽  
Michael D. Weir ◽  
Abraham Schneider ◽  
Ke Ren ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cells

Osteomyelitis and post-operative infections are major problems in orthopedic, dental and craniofacial surgeries.

Decreased Osteogenic Ability of Periodontal Ligament Stem Cells Leading to Impaired Periodontal Tissue Repair in BRONJ Patients

2020 ◽  
Vol 29 (3) ◽  
pp. 156-168 ◽  
Author(s):  
Mengyu Li ◽  
Yejia Yu ◽  
Yueqi Shi ◽  
Yuqiong Zhou ◽  
Wenjie Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Tissue Repair ◽  
Periodontal Tissue ◽  
Periodontal Ligament Stem Cells

scholarly journals Human periodontal ligament stem cells on calcium phosphate scaffold delivering platelet lysate to enhance bone regeneration

RSC Advances ◽  
2019 ◽  
Vol 9 (70) ◽  
pp. 41161-41172 ◽  
Author(s):  
Zeqing Zhao ◽  
Jin Liu ◽  
Michael D. Weir ◽  
Ning Zhang ◽  
Li Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Periodontal Ligament ◽  
Platelet Lysate ◽  
Engineering Applications ◽  
Periodontal Ligament Stem Cells

Human periodontal ligament stem cells (hPDLSCs) are promising for tissue engineering applications but have received relatively little attention.

scholarly journals Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaoxiao Wang ◽  
Yanlan Wang ◽  
Xubin Dai ◽  
Tianyu Chen ◽  
Fanqiao Yang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Parathyroid Hormone ◽  
Periodontal Ligament ◽  
Bone Destruction ◽  
Engineering Applications ◽  
Periodontal Ligament Stem Cells ◽  
Bone Differentiation ◽  
Potential Applications ◽  
Intermittent Pth

Periodontitis is the most common cause of tooth loss and bone destruction in adults worldwide. Human periodontal ligament stem cells (hPDLSCs) may represent promising new therapeutic biomaterials for tissue engineering applications. Stromal precursor antigen-1 (STRO-1) has been shown to have roles in adherence, proliferation, and multipotency. Parathyroid hormone (PTH) has been shown to enhance proliferation in osteoblasts. Therefore, in this study, we aimed to compare the functions of STRO-1(+) and STRO-1(−) hPDLSCs and to investigate the effects of PTH on the osteogenic capacity of STRO-1(+) hPDLSCs in order to evaluate their potential applications in the treatment of periodontitis. Our data showed that STRO-1(+) hPDLSCs expressed higher levels of the PTH-1 receptor (PTH1R) than STRO-1(−) hPDLSCs. In addition, intermittent PTH treatment enhanced the expression of PTH1R and osteogenesis-related genes in STRO-1(+) hPDLSCs. PTH-treated cells also exhibited increased alkaline phosphatase activity and mineralization ability. Therefore, STRO-1(+) hPDLSCs represented a more promising cell resource for biomaterials and tissue engineering applications. Intermittent PTH treatment improved the capacity for STRO-1(+) hPDLSCs to repair damaged tissue and ameliorate the symptoms of periodontitis.

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