Concentration and Activity of the Soluble Form of the Interleukin-7 Receptor   in Type 1 Diabetes Identifies an Interplay Between Hyperglycemia and Immune Function

Abstract Background and aims: Diabetes mellitus (DM) is a multifactorial metabolic disorder characterized by hyperglycaemia caused by insulin deficiency or insulin resistance. It is a global public health problem. This study aimed to determine specific pharmacological effect of quercetin in water soluble and liposomal preparations in experimental diabetes mellitus. Material and methods: We examined the effect of Corvitin and Lipoflavone (at the dose of 10 mg / kg body weight) in a comparative study in white rats with type 1 diabetes and type 2 diabetes coupled with obesity. To simulate the forms of diabetes mellitus most analogous to those in humans we used Streptozotocin at the doses of 30 mg / kg and 50 mg / kg. We tested the levels of glucose, glycosylated hemoglobin, C-reactive protein, and interleukins 6 and 4 in the blood. Results: In animals with type 1 and type 2 diabetes Lipoflavone significantly reduces glucose and glycosylated hemoglobin levels compared to the rats treated with Corvitin. When administered to animals with diabetes, the effect of quercetin in liposomal form on the concentrations of IL-6, IL-4 and Creactive protein is also larger compared to the water-soluble form. Conclusions: Water soluble quercetin preparation Corvitin and to a larger extent liposomal preparation of this flavonoid, Lipoflavone, show anti-inflammatory effect and restore key parameters of carbohydrate metabolism in experimental type 1 diabetes mellitus and type 2 diabetes coupled with obesity, reducing blood glucose and glycosylated hemoglobin levels.

Download Full-text

Interleukin-7 and Type 1 Diabetes

Current Diabetes Reports ◽

10.1007/s11892-014-0518-9 ◽

2014 ◽

Vol 14 (9) ◽

Cited By ~ 13

Author(s):

Paolo Monti ◽

Ezio Bonifacio

Keyword(s):

Type 1 Diabetes ◽

Interleukin 7

Download Full-text

Interleukin-7 receptor α-chain haplotypes differentially affect soluble IL-7 receptor and IL-7 serum concentrations in children with type 1 diabetes

Pediatric Diabetes ◽

10.1111/pedi.12665 ◽

2018 ◽

Vol 19 (5) ◽

pp. 955-962 ◽

Cited By ~ 5

Author(s):

Julia Seyfarth ◽

Christian Lundtoft ◽

Katharina Förtsch ◽

Heinz Ahlert ◽

Joachim Rosenbauer ◽

...

Keyword(s):

Type 1 Diabetes ◽

Serum Concentrations ◽

Interleukin 7 ◽

Α Chain

Download Full-text

Soluble CD163 was linked to galectin-3, diabetic retinopathy and antidepressants in type 1 diabetes

Endocrine Connections ◽

10.1530/ec-18-0336 ◽

2018 ◽

Vol 7 (12) ◽

pp. 1343-1353 ◽

Cited By ~ 5

Author(s):

Eva O Melin ◽

Jonatan Dereke ◽

Maria Thunander ◽

Magnus Hillman

Keyword(s):

Blood Pressure ◽

Type 1 Diabetes ◽

Diabetic Retinopathy ◽

Depression Scale ◽

High Plasma ◽

Soluble Form ◽

Diabetes Clinic ◽

Cross Sectional ◽

Galectin 3

Objective Depression has been associated with diabetic retinopathy and increased plasma levels of galectin-3, a lectin expressed in activated macrophages. Increased levels of sCD163, the soluble form of a macrophage expressed scavenger receptor involved in several inflammatory processes, have been demonstrated in the vitreous of the eye in type 1 diabetes (T1D) patients with severe diabetic retinopathy. The aim was to explore whether circulating sCD163 was associated with diabetic retinopathy, depression and/or galectin-3 in T1D patients, controlling for gender, metabolic factors, other diabetes complications, life style and medication. Design Cross sectional. Methods Two hundred eighty-seven T1D patients, men 56%, age 18–59 years, diabetes duration ≥1 year, were consecutively recruited from one specialist diabetes clinic. Depression was assessed by Hospital Anxiety and Depression Scale-Depression subscale. Blood samples, anthropometrics and blood pressure values were collected, supplemented with data from electronic medical records and the Swedish National Diabetes Registry. High plasma sCD163 was defined as ≥0.575 mg/L (corresponding to the 80th percentile) and high plasma galectin-3 as ≥4.659 µg/L (corresponding to the 95th percentile). Results The prevalence of depression was 10%, antidepressant medication 8%, diabetic retinopathy 72%, high sCD163 20% and high galectin 3 5%. High galectin-3 (AOR 9.7), antidepressants (AOR 3.8), diabetic retinopathy (AOR 2.4) and systolic blood pressure (per mmHg) (AOR 1.03) were associated with high sCD163. Conclusions This is the first study to show that circulating sCD163 was independently associated with galectin-3, the use of antidepressants and diabetic retinopathy, in patients with T1D. Depression was not associated with sCD163.

Download Full-text

Interleukin‐7 dependent non‐classical monocytes and CD40 expression are affected in children with type 1 diabetes

European Journal of Immunology ◽

10.1002/eji.202149229 ◽

2021 ◽

Author(s):

Paul Hehenkamp ◽

Maximilian Hoffmann ◽

Sebastian Kummer ◽

Christina Reinauer ◽

Carsten Döing ◽

...

Keyword(s):

Type 1 Diabetes ◽

Interleukin 7 ◽

Cd40 Expression ◽

Classical Monocytes

Download Full-text

Decreased CD127 Expression on CD4+ T-Cells and Elevated Frequencies of CD4+CD25+CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes

Clinical and Developmental Immunology ◽

10.1155/2013/459210 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 6

Author(s):

Marcin Moniuszko ◽

Barbara Glowinska-Olszewska ◽

Malgorzata Rusak ◽

Marta Jeznach ◽

Kamil Grubczak ◽

...

Keyword(s):

T Cells ◽

Type 1 Diabetes ◽

Cd8 T Cells ◽

Cd4 T Cells ◽

Recent Onset ◽

Interleukin 7 ◽

Inflammatory Parameters ◽

Regulatory Phenotype ◽

Cd127 Expression

Pathobiology of type 1 diabetes (T1D) is predominantly associated with T-cell-related actions. Homeostasis of majority of T-cells is critically dependent on signals mediated by CD127 (interleukin-7 receptor, IL-7R). In contrast, regulatory T-cells express very little CD127 and thereby may be delineated by CD4+CD25+CD127− phenotype. Here we aimed to analyze CD127 expression on CD4+ and CD8+ T-cells and enumerate CD4+CD25+CD127− T-cells in long-lasting T1D. T-cells were analyzed by flow cytometry and immunologic data were correlated with vascular, metabolic, and inflammatory parameters. We demonstrated significantly decreased CD127 levels on CD4+, but not CD8+, T cells in T1D pediatric patients. Interestingly, frequencies of CD4+CD25+CD127− T-cells were significantly enhanced in T1D children and correlated well with frequencies of CD34+CD144+ endothelial progenitor cells and CD4+CD25− T-cells. Levels of CD127 on both CD4+ and CD8+ T-cells in T1D patients were not correlated to each other or HbA1C. Interestingly, however, CD127 levels on CD4+ T-cells were significantly correlated to frequencies of CD4+CD25+CD127− T-cells, whereas CD127 levels on CD8+ T-cells were significantly correlated to concentrations of VEGF and triglycerides. Our data indicate that CD127 expression is differentially modulated on CD4+ and CD8+ T-cells in the course of T1D. Moreover, we demonstrated that, in contrast to recent-onset T1D, long-lasting T1D is associated with enhancement of T-cells with regulatory phenotype.

Download Full-text

Enhancement of the Soluble Form of OX40 and OX40L Costimulatory Molecules but Reduction of the Membrane Form in Type 1 Diabetes (T1D)

Journal of Immunology Research ◽

10.1155/2019/1780567 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Jingnan An ◽

Sisi Ding ◽

Sicheng Li ◽

Lili Sun ◽

Xin Chang ◽

...

Keyword(s):

Flow Cytometry ◽

Type 1 Diabetes ◽

Immune Cells ◽

Peripheral Blood ◽

Clinical Characteristics ◽

Inflammatory Factors ◽

Soluble Form ◽

Healthy Control ◽

First Time

This study analyzed the expression of membrane OX40 and OX40L (mOX40 and mOX40L) and levels of soluble OX40 and OX40L (sOX40 and sOX40L) in T1D patients to determine their clinical significance. Peripheral blood (PB) was collected from patients with T1D and healthy control participants. Expression of mOX40 and mOX40L on immune cells was detected by flow cytometry. Levels of sOX40 and sOX40L in sera were measured by ELISA. We demonstrated for the first time enhanced sOX40 and sOX40L expression and reduced mOX40 and mOX40L levels in T1D patients which correlated with the clinical characteristics and inflammatory factors. These results suggest that OX40/OX40L signal may be promising biomarkers and associated with the pathogenesis of T1D.

Download Full-text