Novel Spatiotemporal Analysis for Exploring a-Cell Differentiation

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2128-P
Author(s):  
MIWA HIMURO ◽  
TAKESHI MIYATSUKA ◽  
LUKA SUZUKI ◽  
MASAKI MIURA ◽  
TAKEHIRO KATAHIRA ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Spatiotemporal Analysis ◽  
A Cell

scholarly journals METTL3-dependent m6A modification programs T follicular helper cell differentiation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yingpeng Yao ◽  
Ying Yang ◽  
Wenhui Guo ◽  
Lifan Xu ◽  
Menghao You ◽  
...  
Keyword(s):  
T Cells ◽  
Transcriptional Regulation ◽  
Cell Differentiation ◽  
Ectopic Expression ◽  
Signature Genes ◽  
Follicular Helper ◽  
Conditional Deletion ◽  
A Cell ◽  
A Site ◽  
Post Transcriptional Regulation

AbstractT follicular helper (TFH) cells are specialized effector CD4+ T cells critical to humoral immunity. Whether post-transcriptional regulation has a function in TFH cells is unknown. Here, we show conditional deletion of METTL3 (a methyltransferase catalyzing mRNA N6-methyladenosine (m6A) modification) in CD4+ T cells impairs TFH differentiation and germinal center responses in a cell-intrinsic manner in mice. METTL3 is necessary for expression of important TFH signature genes, including Tcf7, Bcl6, Icos and Cxcr5 and these effects depend on intact methyltransferase activity. m6A-miCLIP-seq shows the 3′ UTR of Tcf7 mRNA is subjected to METTL3-dependent m6A modification. Loss of METTL3 or mutation of the Tcf7 3′ UTR m6A site results in accelerated decay of Tcf7 transcripts. Importantly, ectopic expression of TCF-1 (encoded by Tcf7) rectifies TFH defects owing to METTL3 deficiency. Our findings indicate that METTL3 stabilizes Tcf7 transcripts via m6A modification to ensure activation of a TFH transcriptional program, indicating a pivotal function of post-transcriptional regulation in promoting TFH cell differentiation.

Activin as a cell differentiation factor

1990 ◽  
Vol 2 (2) ◽  
pp. 113-124 ◽  
Author(s):  
Naoto Ueno ◽  
Shin-ichiro Nishimatsu ◽  
Kazuo Murakami
Keyword(s):  
Cell Differentiation ◽  
Differentiation Factor ◽  
A Cell ◽  
Cell Differentiation Factor

scholarly journals ES cell differentiation system recapitulates the establishment of imprinted gene expression in a cell-type-specific manner

2011 ◽  
Vol 21 (6) ◽  
pp. 1391-1401 ◽  
Author(s):  
C. Kohama ◽  
H. Kato ◽  
K. Numata ◽  
M. Hirose ◽  
T. Takemasa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Differentiation ◽  
Es Cell ◽  
Cell Type ◽  
Imprinted Gene ◽  
Specific Manner ◽  
A Cell ◽  
Cell Type Specific

scholarly journals An endogenous basis for synchronization manners of the circadian rhythm in proliferating Lemna minor plants

2021 ◽  
Author(s):  
Kenya Ueno ◽  
Shogo Ito ◽  
Tokitaka Oyama
Keyword(s):  
Circadian Rhythm ◽  
Circadian Rhythms ◽  
Lemna Minor ◽  
Spatiotemporal Analysis ◽  
Cell Coupling ◽  
Long Distance ◽  
Detached Leaves ◽  
Circadian Organization ◽  
A Cell ◽  
Local Coupling

AbstractEndogenous circadian rhythms in plants play a role in adaptation to day-night cycles. The circadian clock is a cell-autonomous system that functions through the coordination of time information in the plant body. Synchronization of cellular clocks is based on coordination mechanisms; the synchronization manners in proliferating plants remain unclear. We performed spatiotemporal analysis of the circadian rhythm of fronds (leaf-like plant units) of proliferating Lemna minor plants carrying a circadian bioluminescence reporter, AtCCA1:LUC. Noninvasive observations of the bioluminescence of fast-growing two-dimensional plants allowed us to analyze the circadian rhythms at a cell-level resolution and obtain information regarding frond lineage. We focused on spontaneous circadian organization under constant light conditions for plants with light/dark treatment (LD-grown) or without it (LL-grown). Even fronds developing from an LL-grown parental frond showed coherent circadian rhythms among them. This allowed the maintenance of circadian rhythmicity in proliferating plants. Inside a frond, a centrifugal phase/period pattern was observed in LD-grown plants, whereas various phase patterns with traveling waves were formed in LL-grown plants. These patterns were model-simulated by local coupling of cellular circadian oscillators with different initial synchronous states in fronds. Taken together with similar patterning previously reported for detached leaves of Arabidopsis, it is strongly suggested that local coupling is the primary force for the development of these phase patterns in plants lacking long-distance communication. We propose a basic framework of spontaneous phase patterning with three stages of circadian organization: initial phasing, evolution of patterning, and desynchronization/randomizing of phase, in association with altering cell-cell coupling.

Induction of mesenchymal stem cell differentiation by co-culturing with mature cells in double-layered 2-methacryloyloxyethyl phosphorylcholine polymer hydrogel matrices

2021 ◽  
Author(s):  
Kazuhiko Ishihara ◽  
Miu Kaneyasu ◽  
Kyoko Fukazawa ◽  
Ren Zhang ◽  
Yuji Teramura
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Water Soluble ◽  
Polymer Hydrogel ◽  
Differentiated Cells ◽  
Mesenchymal Stem Cell Differentiation ◽  
Hydrogel Matrix ◽  
Zwitterionic Polymer ◽  
A Cell

The effects of differentiated cells on stem cell differentiation were analyzed by co-culture using a cell-encapsulated double-layered hydrogel system. As a polymer hydrogel matrix, a water-soluble zwitterionic polymer having both...

Hematopoietic stem cells from induced pluripotent stem cells – considering the role of microRNA as a cell differentiation regulator

2018 ◽  
Vol 131 (4) ◽  
pp. jcs203018 ◽  
Author(s):  
Aline F. Ferreira ◽  
George A. Calin ◽  
Virgínia Picanço-Castro ◽  
Simone Kashima ◽  
Dimas T. Covas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Hematopoietic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Hematopoietic Stem ◽  
A Cell ◽  
Induced Pluripotent

scholarly journals Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation

2014 ◽  
Vol 19 (4) ◽  
Author(s):  
Retno Nurhayati ◽  
Yoshihiro Ojima ◽  
Naoki Nomura ◽  
Masahito Taya
Keyword(s):  
Oxidative Stress ◽  
Cell Differentiation ◽  
Uv Irradiation ◽  
K562 Cells ◽  
Time Lapse ◽  
Near Ultraviolet ◽  
Initial Stage ◽  
A Cell ◽  
High Ploidy ◽  
G2 Phase

AbstractReactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK differentiation process of K562 cells with phorbol 12-myristate 13-acetate (PMA) induction. A significant increase in the intracellular ROS level was detected on day 1 after near-UV irradiation. In the initial stage of differentiation, a shifted fraction of G1 and G2 phase cells was obtained using near-UV irradiation, giving an increased percentage of G2 phase cells (up from 31.1 to 68.7%). The near-UV irradiation-induced upregulation of the p21 gene, which is a cell cycle inhibitor, suggested that the G2 phase cells were prevented from undergoing cell division. It was found that the percentage of high ploidy (8N and 16N) cells was enhanced significantly at the later stage of the K562 cell culture with near-UV irradiation. Moreover, time-lapse analysis showed that near-UV irradiation encouraged the expression of CD41, a specific surface marker of megakaryocytes. This is the first report that the elevated oxidative stress through the near-UV irradiation promoted the MK differentiation of PMA-induced K562 cells.

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