42-OR: Hub Cells Orchestrate 3-Dimensional Pancreatic Beta-Cell Ca2+ Dynamics In Vivo

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 42-OR
Author(s):  
VICTORIA SALEM ◽  
LUIS F. DELGADILLO SILVA ◽  
KINGA SUBA ◽  
ALDARA MARTIN ALONSO ◽  
WHEI-CHANG KIM ◽  
...  
Keyword(s):  
Beta Cell ◽  
Pancreatic Beta Cell ◽  
3 Dimensional

scholarly journals Involvement of the autonomic nervous system in the in vivo memory to glucose of pancreatic beta cell in rats.

1994 ◽  
Vol 94 (4) ◽  
pp. 1456-1462 ◽  
Author(s):  
J M N'Guyen ◽  
C Magnan ◽  
M C Laury ◽  
C Thibault ◽  
J Leveteau ◽  
...  
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Beta Cell ◽  
Pancreatic Beta Cell ◽  
Autonomic Nervous

Glucose regulates pancreatic [beta] cell Ca2+ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

2018 ◽  
Author(s):  
Victoria Salem ◽  
Kinga Suba ◽  
Aldara Martin-Alonso ◽  
Delgadillo Silva Luis Fernando ◽  
Nadeem Akhtar ◽  
...  
Keyword(s):  
Beta Cell ◽  
Anterior Chamber ◽  
Pancreatic Beta Cell

scholarly journals In vivo and in vitro increased pancreatic beta-cell sensitivity to glucose in normal rats submitted to a 48-h hyperglycaemic period

Diabetologia ◽  
1993 ◽  
Vol 36 (7) ◽  
pp. 589-595 ◽  
Author(s):  
C. Thibault ◽  
C. Guettet ◽  
M. C. Laury ◽  
J. M. N'Guyen ◽  
M. A. Tormo ◽  
...  
Keyword(s):  
Beta Cell ◽  
Pancreatic Beta Cell ◽  
Cell Sensitivity

scholarly journals Defective exocytosis and processing of insulin in a cystic fibrosis mouse model

2019 ◽  
Vol 241 (1) ◽  
pp. 45-57 ◽  
Author(s):  
A Edlund ◽  
M Barghouth ◽  
M Hühn ◽  
M Abels ◽  
J S E Esguerra ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Mouse Model ◽  
Beta Cell ◽  
Cell Function ◽  
Pancreatic Beta Cell ◽  
Hormone Secretion ◽  
Cell Mass ◽  
Beta Cell Mass ◽  
Cell Number

Cystic fibrosis-related diabetes (CFRD) is a common complication for patients with cystic fibrosis (CF), a disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR). The cause of CFRD is unclear, but a commonly observed reduction in first-phase insulin secretion suggests defects at the beta cell level. Here we aimed to examine alpha and beta cell function in the Cftr tm1 EUR/F508del mouse model (C57BL/6J), which carries the most common human mutation in CFTR, the F508del mutation. CFTR expression, beta cell mass, insulin granule distribution, hormone secretion and single cell capacitance changes were evaluated using islets (or beta cells) from F508del mice and age-matched wild type (WT) mice aged 7–10 weeks. Granular pH was measured with DND-189 fluorescence. Serum glucose, insulin and glucagon levels were measured in vivo, and glucose tolerance was assessed using IPGTT. We show increased secretion of proinsulin and concomitant reduced secretion of C-peptide in islets from F508del mice compared to WT mice. Exocytosis and number of docked granules was reduced. We confirmed reduced granular pH by CFTR stimulation. We detected decreased pancreatic beta cell area, but unchanged beta cell number. Moreover, the F508del mutation caused failure to suppress glucagon secretion leading to hyperglucagonemia. In conclusion, F508del mice have beta cell defects resulting in (1) reduced number of docked insulin granules and reduced exocytosis and (2) potential defective proinsulin cleavage and secretion of immature insulin. These observations provide insight into the functional role of CFTR in pancreatic islets and contribute to increased understanding of the pathogenesis of CFRD.

A new perspective of probe development for imaging pancreatic beta cell in vivo

2020 ◽  
Vol 103 ◽  
pp. 3-13 ◽  
Author(s):  
Claire Wen Ying Neo ◽  
Larissa Miasiro Ciaramicoli ◽  
Andreas Alvin Purnomo Soetedjo ◽  
Adrian Kee Keong Teo ◽  
Nam-Young Kang
Keyword(s):  
Beta Cell ◽  
Pancreatic Beta Cell ◽  
New Perspective

Identification of New Pancreatic Beta Cell Targets for In Vivo Imaging by a Systems Biology Approach

2010 ◽  
Vol 16 (14) ◽  
pp. 1609-1618 ◽  
Author(s):  
Thomas Bouckenooghe ◽  
Daisy Flamez ◽  
Fernanda Ortis ◽  
Serge Goldman ◽  
Decio L. Eizirik
Keyword(s):  
Systems Biology ◽  
Beta Cell ◽  
In Vivo Imaging ◽  
Pancreatic Beta Cell
Sign in / Sign up

Export Citation Format

Share Document