scholarly journals Comparison of Sperm Functional Parameters after Cryopreservation of Human Sperm in Three Different Media

2019 ◽  
Vol 4 (02) ◽  
Author(s):  
Sindhuja N. S. ◽  
Manjula G. ◽  
Sanjeeva Reddy N.
Keyword(s):  
Semen Quality ◽  
Semen Analysis ◽  
Male Partner ◽  
Egg Yolk ◽  
Infertility Treatment ◽  
Infertile Couple ◽  
Soy Lecithin ◽  
Semen Cryopreservation ◽  
Functional Parameters ◽  
Commercial Media

Semen cryopreservation is an integral part of infertility treatment in the current day scenario. Recovery of an optimal number of functionally intact spermatozoa from thawed samples has always been the main objective of semen cryopreservation technology. The aim of this study was to compare of Commercial freezing media with Glycerol egg yolk citrate and Soy lecithin media for the cryopreservation of human spermatozoa. This prospective observational study was done at the department of reproductive medicine and surgery, Sri Ramachandra University during the period of August 2015 to January 2016. Male partner of the infertile couple visiting OPD for infertility treatment with normal semen quality (Normozoospermic) were included in the study. Each semen sample was divided into three aliquots and frozen with Commercial media, Glycerol Egg Yolk Citrate (GEYC) media and soy lecithin media. Semen analysis and sperm function tests before freezing and after thawing namely Acrosome reaction, Capacitation and Mitochondrial potential was compared between the groups. There was a significant decline in motility, vitality and functional parameters of the sperms compared to fresh and frozen thawed in all the groups. However, among the frozen thawed groups, the commercial media and GEYC media were comparable in motility and viability parameters;GEYC media was superior to Commercial media in functional sperm parameter maintenance.

scholarly journals Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 653
Author(s):  
Maja Zakošek Pipan ◽  
Margret L. Casal ◽  
Nataša Šterbenc ◽  
Irma Virant Klun ◽  
Janko Mrkun
Keyword(s):  
Semen Quality ◽  
Reproductive Function ◽  
Egg Yolk ◽  
Final Concentration ◽  
Freezing Process ◽  
Soy Lecithin ◽  
Group A ◽  
Short And Long Term

A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and sucrose were added to the control extender at different concentrations to make up the test extenders and final concentration of 50 × 106 spermatozoa/mL. From each group, a 33 µL (1.65 × 106 spermatozoa) suspension of spermatozoa was dropped directly into liquid nitrogen and devitrified at least one week later and evaluated as before. Soy lecithin at 1% and 0.25 M sucrose added to the base vitrification media effectively preserved all sperm qualities. Our results demonstrate the effectiveness of our methods. Vitrification media containing sucrose and soy lecithin cause a minimal decline in quality of canine semen after devitrification. Furthermore, extenders used in our research did not contain egg yolk, which was replaced by soy lecithin, thus allowing for ease of shipping to other countries with strict requirements.

scholarly journals Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium

2014 ◽  
Vol 60 (3) ◽  
pp. 183-188 ◽  
Author(s):  
Srinivas Mutalik ◽  
Sujith Raj Salian ◽  
Kiran Avadhani ◽  
Jyothsna Menon ◽  
Haritima Joshi ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Human Semen ◽  
Soy Lecithin ◽  
Semen Cryopreservation ◽  
Chicken Egg ◽  
Chicken Egg Yolk

scholarly journals Effect of whole and clarified egg yolk based extenders on post-thaw semen quality in bulls.

2021 ◽  
Author(s):  
Prosper Kamusasa ◽  
Eddington Gororo ◽  
Fungayi Primrose Chatiza
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Normal Morphology ◽  
Semen Cryopreservation ◽  
Bull Semen ◽  
Inclusion Level ◽  
Whole Egg

Abstract This study was conducted to evaluate the comparative cryoprotective effects of whole egg yolk and clarified egg yolk on post thaw sperm quality parameters and to determine the optimum clarified egg yolk inclusion level (10-20%) in semen extenders for Mashona bull semen cryopreservation. It was shown that there was a significant decrease in sperm quality variables following cryopreservation. Semen quality increased with the concentration of clarified egg yolk, indicating a positive relationship between egg yolk LDL concentration and maintenance of in vitro sperm quality. The 20% clarified egg yolk (CEY20) extender treatment gave post-thaw motility, viability and normal morphology values which were comparable to the control (20% whole egg yolk, WEY20). The 10% clarified egg yolk concentration gave the least post-thaw quality values and the greatest proportion of defective spermatozoa. This experiment found no advantage of replacing whole egg yolk with up to 15% clarified egg yolk in Mashona bull semen cryopreservation. However, 20% clarified and 20% whole egg yolk performed similarly in the maintenance of post-thaw sperm motility, viability and normal morphology.

scholarly journals Two Protocols of Cryopreservation of Goat Semen with the Use of Computer-Assisted Semen Analysis System

2007 ◽  
Vol 76 (4) ◽  
pp. 601-604 ◽  
Author(s):  
R. Kozdrowski ◽  
A. Dubiel ◽  
W. Bielas ◽  
M. Dzięcioł
Keyword(s):  
Citric Acid ◽  
Semen Analysis ◽  
Egg Yolk ◽  
Computer Assisted ◽  
Semen Cryopreservation ◽  
Velocity Amplitude ◽  
Head Displacement ◽  
Analysis System ◽  
Thawing Procedure ◽  
Lateral Head

The objective of the study was a comparison of two protocols of goat semen cryopreservation with the use of computer-assisted semen analysis system. Twenty ejaculates obtained with electroejaculation method were assessed. Each ejaculate was divided in half and frozen according to two protocols. In protocol I semen was centrifuged in order to remove its plasma and diluted in Tris buffer extender containing glucose, citric acid and glycerol with 20% addition of egg yolk. Protocol II did not include removal of plasma and the extender contained 1.5% egg yolk. It was shown that the removal of semen plasma improved motility of goat spermatozoa following freezing/thawing with respect to the following motility indicators: motility, average path velocity, amplitude of lateral head displacement at p < 0.05, and straight velocity, straightness and linearity at p < 0.01. In conclusion, the removal of semen plasma through centrifugation improved motility properties of goat semen following the freezing/thawing procedure.

Erratum to: 56 SINGLE LAYER CENTRIFUGATION THROUGH PURESPERM® 80 IMPROVES QUALITY OF CRYOPRESERVED DOG SPERMATOZOA

2013 ◽  
Vol 25 (1) ◽  
pp. 175
Author(s):  
L. Alcaráz ◽  
M. Hidalgo ◽  
M. J. Galvez ◽  
D. Acha ◽  
I. Ortiz ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Semen Quality ◽  
Semen Analysis ◽  
Gradient Centrifugation ◽  
Single Layer ◽  
Egg Yolk ◽  
Final Concentration ◽  
Viable Sperm

Density gradient centrifugation with PureSperm® (PureSperm® 40 + PureSperm® 80; Nidacon International, Mölndal, Sweden) has been satisfactorily used to enhance the quality of dog semen samples; however, no studies have been performed on the effect of single layer centrifugation (SLC) with PureSperm® on frozen–thawed dog semen. The aim of this study was to investigate if SLC with PureSperm® 80 can improve the post-thaw semen quality of dog. Semen from 5 dogs was collected by digital manipulation. Two ejaculates from each dog were centrifuged with Tris-based extender, supernatant was removed, and sperm pellet was suspended to a final concentration of 300–400 × 106 sperm mL–1 with CaniPROTM Freeze A plus 20% egg yolk at 22°C. Extended semen was cooled to 5°C within an hour and then diluted to a final concentration of 150–200 × 106 sperm mL–1 in CaniPROTM Freeze B plus 20% egg yolk at 5°C, loaded in 0.5-mL plastic straws and frozen horizontally in ranks placed 4 cm above the surface of liquid nitrogen vapors for 10 min, after which they were directly placed in liquid nitrogen. After 24 to 48 h of storage, straws were thawed in a water bath at 37°C for 30 s. After thawing, semen samples were divided in 2 aliquots: one of them was used as control and the other one was processed by SLC PureSperm® 80. Assessment of sperm motility (assessed by computerized-assisted semen analysis), morphology (Diff-Quick staining), and viability [triple fluorescent stain of propidium iodine/isothiocyanate-labeled peanut (Arachis hypogaea) agglutinin/Rhodamine 123] were evaluated in control and treated semen samples. Data were studied by ANOVA. Results are expressed as mean ± SEM. Significant (P < 0.001) differences were found between SLC-treated and control semen for sperm motility (percentage of total motile spermatozoa: 93.65 ± 0.05 v. 83.79 ± 0.13; percentage of progressive motile spermatozoa: 79.38 ± 6.66 v. 54.61 ± 16.11), morphology (86.45 ± 0.01 v. 83.51 ± 0.01), and viability (percentage of viable sperm with an intact acrosome: 58.32 ± 0.04 v. 36.50 ± 0.17; percentage of viable sperm with an acrosome reaction: 2.81 ± 0.01 v. 9.74 ± 0.21). Based on our results, we can conclude that SLC with PureSperm® 80 is an alternative and successful method for improving the quality of frozen–thawed dog spermatozoa, selecting good-quality spermatozoa (motile, morphologically normal, viable, and acrosome intact spermatozoa) from the rest of the semen sample.

scholarly journals Infertility: Practical Clinical Issues for Routine Investigation of the Male Partner

2020 ◽  
Vol 9 (6) ◽  
pp. 1644 ◽  
Author(s):  
Alberto Ferlin ◽  
Carlo Foresta
Keyword(s):  
Risk Factors ◽  
Semen Analysis ◽  
Male Partner ◽  
Transrectal Ultrasound ◽  
Infertile Couple ◽  
Clinical Approach ◽  
Partner Influence ◽  
Scrotal Ultrasound ◽  
Testicular Histology ◽  
Male Factors

About one-fifth of couples has fertility problems in Western countries. Male factors are present in about half of them, either alone or in combination with female causes. Therefore, both partners should be evaluated simultaneously. The fertility status and/or specific conditions of each partner influence the clinical and treatment approach. This article summarizes in a practical way when, how, and why the male partner of an infertile couple should be investigated. The available evidence and international guidelines were used, interpreting, discussing, and expanding them from personal decades-long experience in this field. The aim is to delineate the most appropriate clinical approach for the male partner of infertile couples, considering traditional and emerging technologies and laboratory analyses in the context of their clinical significance. Components of the initial evaluation in men without known risk factors for infertility should include at minimum medical history, physical examination, and semen analysis. Semen microbiological examination, endocrine assessment, scrotal ultrasound, and transrectal ultrasound are suggested in most men and are mandatory when specific risk factors for male infertility are known to be present or when the initial screening demonstrated abnormalities. Full examination, including genetic tests, testicular histology, or additional tests on sperm, is clinically oriented and/or suggested after the results of initial investigations.

scholarly journals Morphological Image of Fresh and Cryopreserved Dog Semen Evaluated by the Strict Analysis of Sperm Morphology Method, Using Sperm Quality Analyzer (SQA IIc) Evaluation

2006 ◽  
Vol 75 (3) ◽  
pp. 393-401
Author(s):  
P. Přinosilová ◽  
A. Vinkler ◽  
V. Věžník
Keyword(s):  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Egg Yolk ◽  
Term Survival ◽  
Sperm Analysis ◽  
Evaluation Of Parameters ◽  
Membrane Changes ◽  
Sperm Quality Analyzer

Thirty fresh ejaculates from 15 dogs were cryopreserved in Tris-fructose-citric acid-egg-yolk extender with a glycerol content of 6%. Semen samples were examined by the methods of routine sperm analysis and by the SQA IIc device. The routine semen examination focused on the evaluation of parameters determining the quality of sperm membranes. The significance of monitoring semen quality in the course of the short-term survival test for predicting dog semen quality after thawing was assessed. Relevance of the assessment of sperm morphology, and above all the percentage of sperm with membrane changes in the acrosomal region was documented. The fact that the SQA device analyses semen quality by evaluating the mass of moving cells was confirmed. The results provided by the SQA IIc device appear insufficient for the needs of deeper dog semen analysis, especially morphology assessment.

scholarly journals Clinical Spectrum of Infertile Couple: a Retrospective Study at Teaching Hospital

2020 ◽  
Vol 3 (2) ◽  
pp. 375-378
Author(s):  
Hima Rijal ◽  
Suvana Maskey
Keyword(s):  
Semen Analysis ◽  
Male Partner ◽  
Cross Sectional Study ◽  
Clinical Spectrum ◽  
Infertile Couple ◽  
Male Factor ◽  
Cross Sectional ◽  
Chewing Tobacco ◽  
One Year ◽  
Unilateral Block

Introduction: Infertility has been rising steeply as the prime health issue among women around the world these days. This study aims to investigate the causes, hormonal profi le, and clinical spectrum of infertility.Materials and Methods: This is a descriptive cross-sectional study conducted throughout a one year duration in an infertility clinic. The couples meeting the inclusion criteria were included and a pre-formed proforma was used to collect the data regarding history, examination, and investigations.Results: A total of 118 infertile couples were analyzed. The mean age of the females was 28.3±4.5 years. There were 72.1 %cases of primary infertility and 27.9 %of secondary infertility. Regarding obesity status,35.5% were overweight and 15.2 % were obese. Thirty-one (26.2%) males were smokers, 41 (34.7%) used to consume alcohol, and 14 (11.8%) had a habit of chewing tobacco. Among the different fi ndings of semen analysis, 21.1% asthenozoospermia, 9.3% oligoasthenozospermia,7.6% oligospermia, 1.6% azoospermia. Female factor accounted for 45.3%, the malefactor for 28% and in 19.3% the defi nite factor was not determined. The ovulatory disorder was diagnosed in 38.6% of females and hysterosalpingography (HSG) revealed that around 10% had a unilateral block and 1.7% had a bilateral block.Conclusions: Infertility is becoming a global issue affecting a signifi cant number of young couples. About forty-six percent were female aging more than thirty years. The female factor for infertility was more common than the male factor among which ovulatory disorder was the commonest one. Asthenozoospermiawas the commonest abnormal fi nding on semen analysis in a male partner.

Results of semen cryopreservation in young men with testicular carcinoma and lymphoma.

1986 ◽  
Vol 4 (4) ◽  
pp. 537-539 ◽  
Author(s):  
E Reed ◽  
W G Sanger ◽  
J O Armitage
Keyword(s):  
Semen Quality ◽  
Semen Analysis ◽  
Reproductive Potential ◽  
Young Men ◽  
Motile Sperm ◽  
Semen Cryopreservation ◽  
Poor Grade ◽  
Sperm Density ◽  
Testicular Carcinoma ◽  
Patient Groups

Sixty-two young men with testicular carcinoma (31 patients) or lymphoma (31 patients) were referred for semen analysis and possible cryopreservation before systemic therapy. Seventy-seven percent of the patients, 24 patients with testicular carcinoma and 24 patients with lymphoma, had semen with a decreased chance for fertility (ie, sperm density less than 20 X 10(6)/mL and/or less than 40% motile sperm and/or decreased sperm motility). A decreased number of motile sperm as well as a poor grade of motility appeared in the majority of semen specimens from both patient groups. However, 14 patients had semen that met our criteria for fertility with sperm density greater than or equal 20 X 10(6)/mL, greater than or equal 40% motile sperm, and motility grade greater than 2. Semen quality appeared to be better in patients referred immediately after diagnosis than in patients in whom there was a delay between diagnosis and referral for semen cryopreservation. Twelve patients with semen meeting our criteria for possible fertility and 42 patients failing our criteria elected to cryopreserve semen. A median of three collections per patient were stored. To date, seven patients have withdrawn semen for artificial insemination by husband attempts, and two of these attempts have resulted in pregnancies. Both attempts involved patients with semen that meet our criteria for potential fertility. In the minority of young men with lymphoma or testicular carcinoma who have good-quality semen, semen cryopreservation can preserve reproductive potential after therapy that might otherwise cause sterility.

scholarly journals Erratum to: 56 SINGLE LAYER CENTRIFUGATION THROUGH PURESPERM® 80 IMPROVES QUALITY OF CRYOPRESERVED DOG SPERMATOZOA

2013 ◽  
Vol 25 (3) ◽  
pp. 587
Author(s):  
L. Alcaráz ◽  
M. Hidalgo ◽  
M. J. Galvez ◽  
D. Acha ◽  
I. Ortiz ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Semen Quality ◽  
Semen Analysis ◽  
Gradient Centrifugation ◽  
Single Layer ◽  
Egg Yolk ◽  
Final Concentration ◽  
Viable Sperm

Density gradient centrifugation with PureSperm® (PureSperm® 40+PureSperm® 80; Nidacon International, Mölndal, Sweden) has been satisfactorily used to enhance the quality of dog semen samples; however, no studies have been performed on the effect of single layer centrifugation (SLC) with PureSperm® on frozen–thawed dog semen. The aim of this study was to investigate if SLC with PureSperm® 80 can improve the post-thaw semen quality of dog. Semen from 5 dogs was collected by digital manipulation. Two ejaculates from each dog were centrifuged with Tris-based extender, supernatant was removed, and sperm pellet was suspended to a final concentration of 300–400×106spermmL–1 with CaniPROTM Freeze A plus 20% egg yolk at 22°C. Extended semen was cooled to 5°C within an hour and then diluted to a final concentration of 150–200×106spermmL–1 in CaniPROTM Freeze B plus 20% egg yolk at 5°C, loaded in 0.5-mL plastic straws and frozen horizontally in ranks placed 4cm above the surface of liquid nitrogen vapors for 10min, after which they were directly placed in liquid nitrogen. After 24 to 48h of storage, straws were thawed in a water bath at 37°C for 30s. After thawing, semen samples were divided in 2 aliquots: one of them was used as control and the other one was processed by SLC PureSperm® 80. Assessment of sperm motility (assessed by computerized-assisted semen analysis), morphology (Diff-Quick staining), and viability [triple fluorescent stain of propidium iodine/isothiocyanate-labeled peanut (Arachis hypogaea) agglutinin/Rhodamine 123] were evaluated in control and treated semen samples. Data were studied by ANOVA. Results are expressed as mean ± SEM. Significant (P&lt;0.001) differences were found between SLC-treated and control semen for sperm motility (percentage of total motile spermatozoa: 93.65±0.05 v. 83.79±0.13; percentage of progressive motile spermatozoa: 79.38±6.66 v. 54.61±16.11), morphology (86.45±0.01 v. 83.51±0.01), and viability (percentage of viable sperm with an intact acrosome: 58.32±0.04 v. 36.50±0.17; percentage of viable sperm with an acrosome reaction: 2.81±0.01 v. 9.74±0.21). Based on our results, we can conclude that SLC with PureSperm® 80 is an alternative and successful method for improving the quality of frozen–thawed dog spermatozoa, selecting good-quality spermatozoa (motile, morphologically normal, viable, and acrosome intact spermatozoa) from the rest of the semen sample.

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