scholarly journals KERAGAMAN GENETIK, KEBUGARAN DAN INKOMPATIBILITAS REPRODUKSI Hemiptarsenus varicornis Girault (Hymenoptera: Eulophidae), PARASITOID LARVA Liriomyza huidobrensis (Diptera: Agromyzidae)

2011 ◽  
Vol 11 (1) ◽  
pp. 1-10
Author(s):  
Reflinaldon Reflinaldon ◽  
Damayanti Buchori ◽  
Dwinardi Aprianto
Keyword(s):  
Genetic Variation ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Coefficient ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Reproductive Incompatibility ◽  
Liriomyza Huidobrensis ◽  
Geographic Populations ◽  
Polymerase Chain

Several experiments have been conducted to study genetic variation, fitness and reproductive incompatibility of H. varicornis from different geographic populations.  Genetic variation from Pandai Sikek (PS), Alahan Panjang (AP) and Kayu Aro (KA) was analyzed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique and the similarity of genetics measured using NTSys program. The fitness of female wasps such as longevity, fecundity and preoviposition was observed and then compared among those populations.  Incompatibility in reproduction was determined by accounting of reproductive compatibility (RC) index in crossing of intra and interpopulation both of PS and AP.  The results showed high genetic variation of H. varicornis among population from Alahan Panjang, Pandai Sikek and Kayu Aro with similarity coefficient of 30 to 70%.  The best fitness showed the female wasps from Kayu Aro that was significantly different (P= 0.00) in longevity (24.60 ± 6.4 days), fecundity (63.6 ± 28.6 eggs) and parasitization (53.60%) but not significantly different (P=0.07) in number of the first day eggs (1.1 ± 1.4 eggs). Crossing of AP and PS indicated incompatibility in reproduction among the population.

DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

1999 ◽  
Vol 131 (2) ◽  
pp. 229-230 ◽  
Author(s):  
C.K. Chan ◽  
D.J. Petersen ◽  
T.C. Vrain
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Acyrthosiphon Pisum ◽  
Random Amplified Polymorphic Dna ◽  
Aphis Gossypii ◽  
Aphis Fabae ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Laboratory Colonies ◽  
Polymerase Chain

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

scholarly journals Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

2014 ◽  
Vol 61 (4) ◽  
Author(s):  
Anna Biernasiuk ◽  
Izabela Korona-Głowniak ◽  
Agnieszka Grzegorczyk ◽  
Anna Malm
Keyword(s):  
Respiratory Tract ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Small Cell ◽  
Upper Respiratory Tract ◽  
Small Cell Lung ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Upper Respiratory ◽  
Polymerase Chain

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

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