scholarly journals Antibacterial effect of pineapple (Ananas comosus) extract towards Staphylococcus aureus

2018 ◽  
Vol 30 (1) ◽  
pp. 1 ◽  
Author(s):  
Yong Ker Loon ◽  
Mieke Hemiawati Satari ◽  
Warta Dewi
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Herbal Remedy ◽  
Ananas Comosus ◽  
Oral Diseases ◽  
Oral Infections ◽  
Experimental Laboratory ◽  
Reducing Bacteria

Introduction: Staphylococcus aureus is one of the important medical pathogens which have been recognised for many years as a remedyfor a wide case of oral infections. Nowadays, the use of herbal remedy for reducing bacteria in the oral cavity has been implemented widely due to thefewer side effects. Therefore, researchershave been findingwaysto use pineapple in dentistry to prevent many cases of oral diseases. The purpose of this study was to prove that pineapple extract indifferent concentration had the potential as an antibacterial agent towards Staphylococcus aureus. Methods: The study was an experimental laboratory conducted by determining the minimum inhibitory concentration of pineapple (Ananas comosus) with thetwo-fold serial dilution methods. Results:The The Minimum Inhibitory Concentration (MIC) of the pineapple extract was 1.56%-0.78%. Conclusion: The pineapple extract had anantibacterial effect towards Staphylococcus aureus due to the bromelain compound and its phytochemical factor such as Vitamin C and flavonoid. 

scholarly journals Physicochemical and antimicrobial assessment of Iranian Propolis gathered in Qazvin province

2020 ◽  
Vol 10 (2) ◽  
pp. 82
Author(s):  
Fatemeh Samieerad ◽  
Nematollah Gheibi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Standard Strain ◽  
Layer Chromatography

Background: Propolis is one of the useful bee colony products that have been used in traditional medicine for centuries. In this study, the physicochemical characters and their antibacterial effect of Iranian Propolis collected from Qazvin province was assessed.Methods: In this study, Thin Layer Chromatography and Vacuum Liquid Chromatography to detect different compounds of the extract have been used. In the initial evaluation of Propolis extract, it was found that the extract includes variable compounds with different polarity; so, the initial classification of extract with different polarity solvents was essential. Finally, 0.1 gr hydro alcoholic Propolis was injected to the HPLC by ultrasound. The antibacterial effect of Iranian ethanol extract Propolis was measured using a microdilution method against Pseudomonas aeruginosa: P. aeruginosa and Staphylococcus aureus: S.aureus standard strains and the minimum bactericidal and inhibitory concentration were defined.Results: Primary analysis of the ethanol extract by analytical Thin Layer Chromatography, demonstrated the presence of flavonoid and phenol in it. Minimum inhibitory concentration and Minimum Bactericidal Concentration for Staphylococcus aureus: S.aureus standard strain was 2.5mg/ml. The same procedure was done for Pseudomonas aeruginosa: P. aeruginosa standard strain and the Minimum inhibitory concentration and Minimum Bactericidal Concentration were 50mg/ml of Propolis extracts.  Conclusion: According to the results, the alcoholic extract of propolis from Qazvin province of Iran provides significant antimicrobial activity. Its powerful activity may be due to high total phenolic and flavonoid contents.Keywords: Iranian propolis, Antibacterial activity, Phenolic compounds, Flavonoid compound

scholarly journals Sinergitas Aktivitas Antibakteri dari Kelopak Bunga Rosella dan Kitosan Terhadap Staphylococcus aureus

2018 ◽  
Vol 4 (2) ◽  
pp. 93-97
Author(s):  
Haeriah Haeriah ◽  
Natsir Djide ◽  
Gemini Alam ◽  
Sartini Sartini
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Assay Method ◽  
Hibiscus Sabdariffa ◽  
Inhibition Concentration ◽  
Fractional Inhibition ◽  
Mic Value

Currently, the antibacterial research is increasingly promoted primarily from natural materials, due to the increasing number of pathogenic bacteria that have been resistant to existing antibiotics. One of the pathogenic bacteria that has been much resistant to antibiotics is Staphylococcus aureus. Rosella calyx (Hibiscus sabdariffa  L.) and chitosan are known to have antibacterial activity. The aim of this study to find out the antibacterial synergy of the  roselle calyx extract and chitosan against. S.aureus ATCC 33592. Rosella calyx was extracted by maceration using 80 %  ethanol, while chitosan is obtained from deasetilation chitin of shrimp husk. Test antibacterial synergism using checkboard assay method by calculating the minimum inhibitory concentration (MIC)  using microdilution assay. The results showed minimum inhibitory concentration (MIC) value of roselle calyx extract and chitosan were 1250 ppm and 50 ppm, respectively.  MIC value of rosella calyx extract in the presence of chitosan was 625 ppm, while the value of MIC chitosan in the presence of rosella calyx extract was < 0.19 ppm. Fractional Inhibition Concentration Index (FICI) was < 0.5 which concluded that the combination of roselle calyx extract with chitosan has a synergistic antibacterial effect on S.aureus ATCC 33592.

scholarly journals Evaluation of inhibitory efficacy of ginger extract on Staphylococcus aureus bacteria: تقييم الفعالية التثبيطية لمستخلص الزنجبيل على بكتيريا Staphylococcus aureus

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Shaimaa Zaidoun Nafi Mohammed, Wael Yassin Al Dabbagh
Keyword(s):  
Staphylococcus Aureus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Ginger Extract ◽  
Inhibition Concentration

This study aimed was to evaluate the effect of microbial antibody on water ginger extract and to determine its inhibitory effect on Staphylococcus aureus bacteria which cause urinary tract infection concentrations of the extract 100% , 75% , 50% ,25 % mg / ml) were used and the minimum inhibitory concentration of the extract was measured . The results showed that Minimum inhibition concentration   (MIC) of the ginger extract was 25% .the water extract effective against the bacteria . the results were compared with the effect of certain antibiotics such as Nitrofurantionl (F), Gentamycin (CN) , Ciprofloxacin (CIP), Trimethoprim (TMP) . The effect of ginger extract has had a better antibacterial effect than some antibiotics.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

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