scholarly journals Acoustic Detection of Black Vine Weevil, Otiorhynchus sulcatus (Fabricius) (Coleoptera: Curculionidae) Larval Infestations in Nursery Containers

2002 ◽  
Vol 20 (3) ◽  
pp. 166-170
Author(s):  
R. W. Mankin ◽  
J. R. Fisher
Keyword(s):  
Early Detection ◽  
Acoustic Detection ◽  
Acoustic System ◽  
High Likelihood ◽  
User Friendliness ◽  
Detection Systems ◽  
Late Fall ◽  
Otiorhynchus Sulcatus ◽  
Black Vine Weevil ◽  
Nursery Crops

Abstract Acoustic detection systems have been developed to locate and target hidden infestations of root weevil larvae in container-grown nursery crops. Tests were conducted in laboratory and field environments with natural and artificial infestations of Otiorhynchus sulcatus (Fabricius) in containers with different nursery plants to determine whether the larvae were large enough for acoustic detection during late fall when scouting for infestation often occurs at commercial nurseries in Oregon. The rootballs of tested plants were examined to verify the presence or absence of larvae. All of the containers rated at high likelihood of infestation contained O. sulcatus larvae (29% of those tested). No larvae were found in any containers rated at low likelihood of infestation (20%). Sporadic sounds were detected but failed to exhibit periodicity suggestive of infestation in 51% of the containers (rated at medium likelihood). Fifty seven percent of these medium-rated containers were infested. Experience with the use of the acoustic system in field environments suggested improvements in user-friendliness and robustness that could improve its utility for early detection and targeting applications in commercial nurseries.

scholarly journals Efficacy of Metarhizium anisopliae as a Curative Application for Black Vine Weevil (Otiorhynchus sulcatus) Infesting Container-Grown Nursery Crops

2007 ◽  
Vol 25 (3) ◽  
pp. 150-156 ◽  
Author(s):  
Denny J. Bruck
Keyword(s):  
Environmental Protection Agency ◽  
Metarhizium Anisopliae ◽  
Fungal Growth ◽  
Egg Laying ◽  
Effect Of Temperature ◽  
Speed Of Kill ◽  
Otiorhynchus Sulcatus ◽  
Black Vine Weevil ◽  
Nursery Crops ◽  
The Impact

Abstract The black vine weevil (BVW), Otiorhynchus sulcatus (F.) is a serious pest of nursery crops. The fungus, Metarhizium anisopliae (F52), is registered by the U.S. Environmental Protection Agency for BVW control. The objectives of this study were to determine the efficacy of a curative drench application of M. anisopliae for controlling BVW larval infestations in container-grown nursery plants and the effect of temperature on the rate of fungal growth and speed of kill. Trials evaluating the efficacy of M. anisopliae as a curative application were performed in the spring of 2004 and 2005 as well as the fall of 2006. Laboratory studies were performed to quantify the impact of temperature (10, 15, 20, 24 and 28C) on fungal growth and speed of kill. Metarhizium anisopliae applied in the greenhouse and outdoors in 2004 were 92 and 30% effective, respectively. Fungal applications to container-grown plant material maintained outdoors in the spring of 2005 were nearly 100% effective 28 days after application. Fall applications in 2006 provided statistically significant reductions in the number of live BVW larve per pot, but were not as effective as spring applications in 2005. The mean media temperature of containers maintained outdoors in the fall of 2006 dropped considerably (10–12C) over the course of the experiment and were likely the cause for the reduced efficacy. Laboratory experiments demonstrated that temperatures below 20C (68F) significantly slowed fungal growth and the speed at which M. anisopliae infected BVW larvae. In the field, drench applications of M. anisopliae were very effective at eliminating BVW larvae in container-grown nursery plants when media temperatures were adequate (> 15C (59F)). The use of M. anisopliae as a curative drench application has similar temperature-dependent limitations as the use of entomopathogenic nematodes for BVW control. Therefore, applications should occur as early in the fall as possible once egg laying has ended or in late spring just prior to pupation when media temperatures would be most conducive to fungal infection.

Black Vine Weevil (Coleoptera: Curculionidae) Performance in Container- and Field-Grown Hosts

2008 ◽  
Vol 43 (3) ◽  
pp. 300-310 ◽  
Author(s):  
Michael E. Reding
Keyword(s):  
Plant Species ◽  
Field Trial ◽  
Adult Stage ◽  
Bare Soil ◽  
Small Field ◽  
Restricted Diet ◽  
Otiorhynchus Sulcatus ◽  
Black Vine Weevil ◽  
Nursery Crops ◽  
Field Plots

The black vine weevil (Otiorhynchus sulcatus F.) feeds on a variety of plant species and is a serious pest of ornamental nursery crops. The larval stage has a more restricted diet than the adult stage, but the larvae are more damaging because they feed on roots and often stunt or kill their hosts. Performance and establishment of larvae in various container- and field-grown nursery crops were examined. In trials on containerized plants, adult black vine weevil were caged with various combinations of known larval hosts. In these trials, most larvae were found in Sedum and Heuchera versus Astilbe, redbud, and Taxus. Larvae collected from Sedum weighed significantly more than those from Heuchera or Taxus. In one field trial, adult black vine weevils were released, and establishment of larvae among potential hosts was examined. In this trial, Sedum was a better host than hemlock or Rudbeckia. Based on the numbers of larvae found in the different plant species, Sedum was the best host for larvae. Another field trial examined the influence of mulch on the occurrence of larvae. The presence of mulch around small field-grown Taxus plants did not increase the numbers of larvae compared with bare soil. The presence of aged pine bark or Taxus needle mulches did not influence the occurrence of black vine weevils in field plots of young Taxus. Data from monitoring adults suggest they either emigrated from these plots within several weeks of emergence, although suitable hosts were present, or there was high mortality.

Otiorhynchus sulcatus. [Distribution map].

1990 ◽  
Author(s):  
Keyword(s):  
New York ◽  
North Carolina ◽  
Rhode Island ◽  
Pacific Islands ◽  
Distribution Map ◽  
Otiorhynchus Sulcatus ◽  
Black Vine Weevil ◽  
St Helena ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Otiorhynchus sulcatus (Fabricius) Coleoptera: Culculionidae (vine weevil, strawberry, cyclamen or black vine weevil). Attacks strawberry, grapevine, Cyclamen. Information is given on the geographical distribution in Europe, Austria, Belgium, Czechoslovakia, Denmark, Finland, France, Germany, Hungary, Ireland, Italy, Malta, Netherlands, Norway, Poland, Sweden, Switzerland, United Kingdom, Yugoslavia, USSR, Russian SFSR, European USSR, Africa, St. Helena, Egypt, Asia, Japan, Australasia and Pacific Islands, Australia, Tasmania, Chatham Islands, Hawaii, New Zealand, North America, Canada, British Columbia, New Brunswick, Newfoundland, Nova Scotia, Ontario, Quebec, USA, Alaska, Arizona, California, Connecticut, District of Columbia, Idaho, Illinois, Kansas, Maine, Maryland, Massachusetts, Michigan, Missouri, Montana, Nevada, New Hampshire, New Jersey, New Mexico, New York, North Carolina, Ohio, Oregon, Pennsylvania, Philadelphia, Rhode Island, South Dakota, Utah, Vermont, Virginia, Washington, Wisconsin, South America, Chile, Colombia.

scholarly journals Highly sensitive biomarker biosensor for early detection of cancer biomarkers

2021 ◽  
Author(s):  
Jaydev Upponi ◽  
Tiziana Musacchio ◽  
Salome Siavoshi ◽  
Asanterabi Malima ◽  
Cihan Yilmaz ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Early Detection ◽  
Carcinoembryonic Antigen ◽  
Early Detection Of Cancer ◽  
Cancer Biomarkers ◽  
Diagnostic Tools ◽  
Detection Systems ◽  
Highly Sensitive ◽  
Current Detection ◽  
Insufficient Sensitivity

Abstract Biomarkers are emerging as potentially important diagnostic tools for cancer and many other diseases. However, many current detection systems for suffer from insufficient sensitivity. To address this concern, we developed a highly sensitive biosensor, featuring monoclonal antibody-coated polystyrene nanobeads assembled in the trenches of a microchip, for the detection of cancer biomarkers. These biosensors detected nucleosomes and carcinoembryonic antigen in serum at concentrations of 62.5 and 15.6 pg/mL, respectively. Very low detection limits that suggest such devices might be beneficial for the early detection of tumors and for monitoring of patients in remission.

Use of a cold-active entomopathogenic nematode Steinernema kraussei to control overwintering larvae of the black vine weevil Otiorhynchus sulcatus (Coleoptera: Curculionidae) in outdoor strawberry plants

Nematology ◽  
2002 ◽  
Vol 4 (8) ◽  
pp. 925-932 ◽  
Author(s):  
Rodney Edmondson ◽  
Paul Richardson ◽  
Deena Willmott ◽  
Andrew Hart ◽  
Steve Long
Keyword(s):  
Low Temperatures ◽  
Prolonged Exposure ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Extraction Methods ◽  
Otiorhynchus Sulcatus ◽  
Cold Active ◽  
Clear Dose Response ◽  
Black Vine Weevil ◽  
Baermann Funnel

AbstractThe susceptibility of overwintering black vine weevil larvae Otiorhynchus sulcatus to a cold-active entomopathogenic nematode, Steinernema kraussei (isolate L137) and a commercial proprietary biopesticide, Steinernema carpocapsae (Exhibit) was assessed on outdoor potted strawberry plants. Nematodes were applied at a range of doses in early winter at a field site located in Warwickshire, England. Three months later, infestation and mortality of vine weevils were assessed. There was a clear dose response observed for S. kraussei applications. Up to 81% of vine weevil control was recorded by this nematode at the highest dose of 60 000 nematodes per pot, whereas treatments with S. carpocapsae caused no significant mortality at the dose rates used. Dissection of vine weevil larvae showed infective juveniles had developed to adults within the host. Recovery of nematodes at the end of the experiment indicated that S. kraussei (L137) was able to survive winter field conditions including prolonged exposure to low temperatures (averaging 2.7°C during the experiment) in contrast to S. carpocapsae which showed poor survival. Of the two extraction methods used, the Baermann funnel technique was found to be more efficient than Galleria mellonella baiting, with up to 44% of the original highest dose of S. kraussei nematodes being recovered by Baermann funnel compared to 8% with G. mellonella at 18°C. These results suggest S. kraussei (L137) has potential as a commercial biocontrol agent against O. sulcatus at low temperatures.

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