scholarly journals Auxin Application to Stem Cuttings of Selected Woody Landscape Plants by Incorporation Into a Stabilized Organic Rooting Substrate

2004 ◽  
Vol 22 (2) ◽  
pp. 63-70
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt ◽  
John M. Ruter

Abstract Stem cuttings of Buxus sinica var. insularis ‘Wintergreen’, Elaeagnus x ebbingei, Ficus benjamina, Gardenia augusta ‘Radicans’, Ilex glabra ‘Nigra’, Ilex vomitoria ‘Nana’, Juniperus conferta ‘Blue Pacific’, Ternstroemia gymnanthera, and Trachelospermum asiaticum were inserted into a stabilized organic rooting substrate (plugs comprised of peat and a polymer binder) that had been soaked in water, aqueous solutions of K-IBA (15 to 75 ppm), or K-IBA + K-NAA (15 ppm + 7.5 ppm to 60 ppm + 30 ppm). Rooting and initial shoot growth responses were compared with cuttings receiving a basal quick-dip in K-IBA (1000 ppm) or K-IBA + K-NAA (1000 ppm + 500 ppm). Rooting percentage, number of roots per rooted cutting, and total root length per rooted cutting for cuttings rooted in auxin-treated plugs were similar to or greater than cuttings receiving a basal quick-dip; lesser results were obtained in a few cases with K-IBA + K-NAA. Percent of rooted cuttings with new shoots and shoot length per rooted cutting for cuttings rooted in plugs treated with K-IBA were mostly similar to cuttings receiving a basal quick-dip in K-IBA, while cuttings rooted in plugs treated with K-IBA + K-NAA exhibited similar or lesser results compared to cuttings receiving a basal quick-dip in K-IBA + K-NAA.

2007 ◽  
Vol 25 (3) ◽  
pp. 126-130
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley

Abstract Stem cuttings of Abelia ‘Edward Goucher’, Buxus sinica var. insularis ‘Wintergreen’, Hedera helix, Hibiscus syriacus ‘Collie Mullens’, Ilex vomitoria ‘Nana’, Juniperus conferta ‘Blue Pacific’, and Rosa ‘Red Cascade’ received a basal quick-dip in solutions of Dip ‘N Grow at concentrations of 0 + 0, 50 + 25, 250 + 125, 500 + 250, 750 + 375, and 1000 + 500 ppm IBA + NAA prepared with and without 13.5 g/liter sodium cellulose glycolate (SCG) as a thickening agent. Cuttings of Ilex vomitoria ‘Nana’ exhibited increased rooting with increasing auxin concentration with inclusion of SCG. The other six taxa exhibited similar rooting percentages among all treatments, but exhibited an increase in root number and/or total root length with inclusion of SCG. Initial shoot growth on rooted cuttings of Abelia ‘Edward Goucher’ showed some reduction with increasing auxin concentration with inclusion of SCG, suggesting cuttings absorbed more auxin from solutions containing SCG owing to their extended period of exposure to the auxin. Otherwise, there were no negative responses to solutions containing SCG. The greater viscosity of solutions prepared with SCG can help reduce the possibility of spillage and evaporation of alcohol during use of the auxin solutions.


2005 ◽  
Vol 23 (2) ◽  
pp. 67-71
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt ◽  
Bertram Zinner

Abstract Stem cuttings of Hydrangea paniculata Sieb., Rosa L. ‘Red Cascade’, Salvia leucantha Cav., and Solenostemon scutellarioides (L.) Codd ‘Roseo’ were inserted into six rooting substrates: monolithic slag, sand, perlite, vermiculite, Fafard 3B, or fine pine bark. Rooting, initial shoot growth, and ease of dislodging substrate particles from root systems upon bare-rooting by shaking and washing cuttings rooted in monolithic slag were compared to cuttings rooted in the five other substrates. Rooting percentage, number of primary roots per rooted cutting, and total root length per rooted cutting for cuttings rooted in monolithic slag were generally similar to the five other substrates. Particles of monolithic slag were dislodged more readily from root systems by shaking than were the other substrates. Gentle washing removed almost all particles of monolithic slag and sand from the root systems of all taxa and removed almost all particles of pine bark from all taxa except S. scutellarioides ‘Roseo’. Monolithic slag had a bulk density similar to sand, retained less water than the other substrates, and was similar to perlite, vermiculite, and pine bark in particle size distribution.


2004 ◽  
Vol 22 (2) ◽  
pp. 55-57
Author(s):  
Jason J. Griffin ◽  
Kenneth R. Schroeder

Abstract Stem cuttings of Ulmus parvifolia Jacq. ‘Emerald Prairie’ (‘Emerald Prairie’ lacebark elm), consisting of 7.5 to 10 cm (3 to 4 in) terminal shoot portions, were collected four times throughout the year from mature stock plants and treated with the potassium (K) salt of indole-3-butyric acid (K-IBA) ranging from 0 to 20,000 ppm (2.0%). Rooting percentages were affected greatly by K-IBA treatment and stock plant growth stage. Little to no rooting was observed without K-IBA application, whereas overall rooting for treated softwood, early semi-hardwood, late semi-hardwood, and hardwood, cuttings was 92, 86, 87, and 8%, respectively. Mean root number varied by growth stage and K-IBA concentration. Higher concentrations of K-IBA increased the number of roots on rooted cuttings except for hardwood cuttings. In general, rooting percentage and mean root number were highest at the softwood stage with 15,000 or 20,000 ppm (1.5 or 2.0%) K-IBA, resulting in 97% rooting and 15 or 22 roots per rooted cutting, respectively. However, leaf abscission was high on softwood cuttings. Semi-hardwood cuttings achieved similar rooting percentages and may exhibit better survival and growth due to greater leaf retention.


2012 ◽  
Vol 22 (4) ◽  
pp. 476-478
Author(s):  
Eugene K. Blythe

Confederate rose (Hibiscus mutabilis), a native of southeastern China, is an old-fashioned, ornamental plant often found in older gardens in the southern United States. Current breeding programs aim at developing selections with improved garden performance, thus providing new cultivars for nursery production. Hardy in U.S. Department of Agriculture (USDA) zones 7 to 9, plants grow as large shrubs or small trees in warmer areas, but generally die back to a woody base or short trunk in colder areas of their range. Stems from the past growing season that remain on plants during the winter in the warmer regions may be used to prepare hardwood stem cuttings. The current study examined hardwood cutting propagation of confederate rose in response to a 1-second basal quick-dip in auxin [1000 ppm indole-3-butyric acid (IBA), 3000 ppm IBA, 1000 ppm IBA + 500 ppm 1-naphthaleneacetic acid (NAA), and 3000 ppm IBA + 1500 ppm NAA] and a basal wound (along with 1000 ppm IBA only). Cuttings were rooted in a warm, high-humidity environment within a greenhouse. Auxin treatments improved overall rooting percentage and total root length, with 1000 ppm IBA (without and with a basal wound) providing the highest rooting percentages (about 70%) and nontreated cuttings the lowest (44%). A significant increase in total root length on rooted cuttings resulted with the use of 3000 ppm IBA (211 cm) and use of a basal wound plus 1000 ppm IBA (193 cm) compared with nontreated cuttings (87 cm). Auxin and wounding treatments did not have any significant inhibitory effects on budbreak and growth of new shoots on rooted cuttings.


2003 ◽  
Vol 21 (3) ◽  
pp. 131-136 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt ◽  
John M. Ruter

Abstract Research was conducted to determine whether a foliar spray application of the commercial root-promoting formulation Dip 'N Grow® [10,000 ppm indole-3-butyric acid (IBA) and 5,000 ppm 1-naphthaleneacetic acid] or the potassium (K) salt of IBA (K-IBA) would be as effective as a basal quick-dip for rooting stem cuttings of selected ornamentals. Cuttings of Chrysanthemum pacficum sprayed with Dip 'N Grow® at 50 + 25 ppm IBA + NAA or K-IBA at 0 to 50 ppm showed generally similar root development measures compared to a basal quick-dip; an exception was 10 ppm K-IBA which produced greater total root length. Cuttings of Forsythia x intermedia ‘Lynwood Gold’ typically did not differ in root development under the two application methods, but cuttings sprayed with Dip 'N Grow® at 0 + 0 to 10 + 5 ppm IBA + NAA exhibited greater shoot growth 80 days after sticking (insertion into the rooting substrate) than cuttings receiving a basal quick-dip. Root and shoot development measures were similar or lower for cuttings of Abelia x grandiflora, Hydrangea paniculata, and Lagerstroemia (indica x fauriei) ‘Natchez’ sprayed with auxin compared to a basal quick-dip.


2017 ◽  
Vol 27 (1) ◽  
pp. 69-72
Author(s):  
Justin A. Schulze ◽  
Ryan N. Contreras ◽  
Carolyn F. Scagel

‘Schipkaensis’ common cherrylaurel (Prunus laurocerasus) is an important nursery crop across the United States. In our breeding efforts to reduce shot-hole symptoms and weediness, we have created chromosome doubled forms of this cultivar. Vegetative propagation is an important factor in nursery production, and we have found no studies that have looked at comparative adventitious rooting of stem cuttings using induced polyploids. The objective of this research was to determine if rooting ability varied between these two ploidy levels. Semihardwood stem cuttings from wild-type (22x) and polyploid (44x) ploidy levels were taken at the end of July 2015 and the beginning of July 2016. Cuttings were dipped in 1030 ppm (0.10%) indole-3-butyric acid (IBA) and 660 ppm (0.066%) 1-naphthaleneacetic acid (NAA) before being set in rooting substrate. After 1 month, cuttings were removed from substrate and data collected. Data included; rooting percentage, root number per rooted cutting, average root length, and total root length. In 2015, 88% of the cuttings from the 44x plants and 63% of the cuttings from the 22x plants rooted. In 2016, 100% of cuttings from both ploidy levels rooted. In both years, average root length and total root length were similar between ploidy levels; however, cuttings from 22x plants generally had more roots than those from 44x. Chromosome-doubled ‘Schipkaensis’ common cherrylaurel rooted effectively, and produce transplantable cuttings similar to the standard ploidy.


1999 ◽  
Vol 17 (2) ◽  
pp. 53-56
Author(s):  
Daniel K. Struve ◽  
L. Mark Lagrimini

Abstract Japanese Stewartia, Stewartia pseudocamellia (Maxim.), cuttings were rooted and seeds germinated to compare growth and survival of the two plant types. Softwood cuttings collected from 18-month-old, 1 to 1.3 m (3 to 4 ft) tall stock plants were rooted successfully (greater than 64%) at two propagation times by giving severed cuttings a basal quick dip in 0.1 M ascorbic or caffeic acid, before a basal quick dip in 100 ppm indolebutyric acid (IBA). Cuttings were overwintered in either a minimum heat (3C, 35F) polyhouse (89% survival), or in a 7C (45F) cooler (97% survival). There was no difference in height following overwintering, but by the end of the first growing season following propagation, seedlings were significantly taller than rooted cuttings, 84 vs 72 cm (33 vs 28 in), respectively. Although growth was vigorous, survival after upcanning was poor; rooted cutting survival was 17%, seedling survival was 48%. This study demonstrates that Japanese Stewartia can be propagated readily by softwood and semi-hardwood stem cuttings dipped in low IBA concentrations if cuttings are pre-treated with either ascorbic or caffeic acid. These cuttings can also be overwintered successfully.


HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1052A-1052
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt

Stem cuttings of Hydrangea paniculata Sieb., Rosa L. `Red Cascade', Salvia leucantha Cav., and Solenostemon scutellarioides (L.) Codd `Roseo' were inserted into six rooting substrates: monolithic slag [(MgFe)2Al4Si5O18], sand, perlite, vermiculite, Fafard 3B, or fine pine bark. Rooting, initial shoot growth, and ease of dislodging substrate particles from root systems upon bare-rooting by shaking and washing cuttings rooted in monolithic slag were compared to cuttings rooted in the five other substrates. Rooting percentage, number of primary roots per rooted cutting, and total root length per rooted cutting for cuttings rooted in monolithic slag were generally similar to the five other substrates. Particles of monolithic slag were dislodged more readily from root systems by shaking than were the other substrates. Gentle washing removed almost all particles of monolithic slag and sand from the root systems of all taxa and removed almost all particles of pine bark from all taxa except S. scutellarioides `Roseo'. Monolithic slag had a bulk density similar to sand, retained less water than the other substrates, and was similar to perlite, vermiculite, and pine bark in particle size distribution. Our studies indicate that monolithic slag, where regionally available, could provide a viable material for producing bare-root cuttings.


2009 ◽  
Vol 19 (1) ◽  
pp. 130-132 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley

‘Dwarf Burford’ holly (Ilex cornuta ‘Dwarf Burford’) is a significant nursery crop and is widely used in landscapes in U.S. Department of Agriculture hardiness zones 7 to 9. Stem cuttings can be rooted at multiple times during the year, provided cutting wood is sufficiently mature, with auxin treatments traditionally used to encourage rooting. This study was conducted to determine if auxin treatment could be eliminated, thus reducing labor and chemical requirements in the cutting propagation process. In three experiments, terminal stem cuttings of ‘Dwarf Burford’ holly were taken in winter, prepared with and without use of a basal quick-dip in an auxin solution, and rooted in a warm, high-humidity environment. Rooting percentages for nontreated cuttings and cuttings treated with 2500 ppm indole-3-butyric acid (IBA) + 1250 ppm 1-naphthaleneacetic acid (NAA) were similar, while treatment of cuttings with 5000 ppm IBA + 2500 ppm NAA resulted in a decrease in rooting percentage. The number of primary roots and total root length were similar among the three treatments, except in one experiment where total root length was greater with auxin-treated cuttings than with nontreated cuttings. Initial shoot growth responses were variable among the three experiments. The treatment of cuttings with auxin was not required for successful rooting and can be eliminated from the process for winter stem cutting propagation of ‘Dwarf Burford’ holly.


1998 ◽  
Vol 22 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Barry Goldfarb ◽  
Scott E. Surles ◽  
Mack Thetford ◽  
Frank A. Blazich

Abstract Stem cuttings of loblolly pine (Pinus taeda L.) were rooted in a greenhouse and then scored for the number of roots per rooted cutting, the number of vertically oriented roots per rooted cutting, and symmetry of the arrangement of adventitious roots on the lower stem. Rooted cuttings were transplanted to a nursery bed, grown for 7 months, lifted and rescored, transplanted to a field location, and then measured after 1 yr in the field. Shoot height after the rooting period was correlated weakly with the number of roots per rooted cutting, but not with the number of vertical roots or root system symmetry. Nursery culture slightly reduced the number of roots per cutting and root system symmetry. Root orientation changed dramatically as roots elongated, with 94% of all roots scored as vertical after nursery growth. Shoot height after the period of nursery growth was still correlated weakly with root number, but not with the number of vertical roots. Rooted cuttings with symmetrical root systems were slightly taller than cuttings with asymmetrical root systems after growth in the nursery. After 1 yr in the field, shoot height was no longer correlated with root number. On average, cuttings with symmetrical root systems were only 2 mm taller than cuttings with asymmetrical root systems. These early growth data suggest it is not beneficial to impose culling criteria for cuttings rooted in a greenhouse and transplanted to a nursery based on the root system architecture at the time of rooting. However, growth and stability of rooted cuttings over a longer time period must be assessed. South. J. Appl. For. 22(4): 231-234.


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