scholarly journals Trichoderma Asperellum, an inoculant for production of steviol glycoside in Stevia Rebaudiana bertoni plants micropropagated in a temporary immersion bioreactor

2020 ◽  
Vol 19 (3) ◽  
pp. 1153-1161 ◽  
Author(s):  
"D. " Villamarín-Gallegos ◽  
◽  
"D. G. " Oviedo-Pereira ◽  
"S. " Evangelista-Lozano ◽  
"G. " Sepúlveda-Jiménez ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Trichoderma Asperellum ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

Daminozide Enhances Vigor and Steviol Glycoside Yield of Stevia (Stevia rebaudiana Bert.) Propagated in the Temporary Immersion Bioreactor

2022 ◽  
Author(s):  
Rizka Tamania Saptari ◽  
Rizkita Rachmi Esyanti ◽  
Riza Arief Putranto
Keyword(s):  
Stevia Rebaudiana ◽  
Feedback Regulation ◽  
Optimum Concentration ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Steviol Glycoside ◽  
Kaurenoic Acid ◽  
Biosynthesis Gene ◽  
Inhibitor Treatment ◽  
Ga Biosynthesis

Abstract Stevia (Stevia rebaudiana Bertoni) contains sweet compound widely used as natural sweetener, steviol glycoside (SG). SG is a diterpenoid secondary metabolite synthesized from ent-kaurenoic acid, the same precursor of Gibberellin (GA). Therefore, in this study, a GA inhibitor, Daminozide (0, 10, 20 ppm) was used to block ent-kaurenoic acid conversion towards GA synthesis in attempt to increase SG content of stevia propagated in Temporary Immersion Bioreactor (TIB). Daminozide in 10 mg/L was observed to be the optimum concentration which increased biomass weight and SG content (stevioside and rebaudioside A) up to 40%. The treatment also increased transcripts accumulation of genes enrolled in SG biosynthesis, such as SrKA13H, SrUGT85C2, and SrUGT76G1, indicating SG pathway become more active due to the inhibition of GA pathway. Furthermore, the inhibition of GA was also indicated by the upregulated expression of GA biosynthesis gene (GA3ox) as the result of feedback regulation, and the downregulated expression of GA catabolism gene (GA2ox2) as the result of feed-forward regulation caused by inhibitor treatment.

Salt-tolerance mechanisms induced in Stevia rebaudiana Bertoni: Effects on mineral nutrition, antioxidative metabolism and steviol glycoside content

2017 ◽  
Vol 115 ◽  
pp. 484-496 ◽  
Author(s):  
Daniel Cantabella ◽  
Abel Piqueras ◽  
José Ramón Acosta-Motos ◽  
Agustina Bernal-Vicente ◽  
José A. Hernández ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Mineral Nutrition ◽  
Stevia Rebaudiana ◽  
Tolerance Mechanisms ◽  
Antioxidative Metabolism ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

scholarly journals Pertumbuhan, Produksi Biomassa, dan Kandungan Glikosida Steviol pada Lima Klon Stevia Introduksi di Bogor, Indonesia

2019 ◽  
Vol 47 (1) ◽  
pp. 105-110
Author(s):  
Masna Maya Sinta ◽  
Dan Sumaryono
Keyword(s):  
High Performance ◽  
Biomass Yield ◽  
Stevia Rebaudiana ◽  
Growth And Yield ◽  
Plant Canopy ◽  
Rebaudioside A ◽  
Plant Mortality ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Leaf Yield

Stevia rebaudiana Bertoni, a sweetener plant, has been cultivated in Indonesia since the early 1980s, but the yield is lower than in other countries. Five selected introduced stevia clones were planted at Megamendung, Bogor, Indonesia (6°39’ S, 106°56’ E, 800 m above sea level) on December 2014 until November 2015 to evaluate their growth and biomass yield. The growth and yield were observed from 6 to 12 months after planting. In addition, stevioside, rebaudioside A (reb A), and total steviol glycoside (TSG) contents of leaves were measured using high performance liquid chromatography (HPLC). At six months after planting, stem diameter was 8-11 mm, plant canopy diameter was 23-30 cm, plant height was 24-35 cm, and plant mortality was less than 5% except for clone BU at 30.7% due to Sclerotium attack. The plants flowered at different times, therefore had different harvest durations, from 4 weeks (clone BM) to 8 weeks (clone BP). The highest leaf yield was clone BM (6.04 ton ha-1 per year), followed by BX (4.91 ton ha-1 per year), and BP (4.38 ton ha-1 per year). From the five clones tested, clone BM was the best for leaf, TSG, and stevioside yields; whereas clone BS and BP were the best for reb A yield. Keywords: rebaudioside A, Stevia rebaudiana, stevioside, total steviol glycoside

Optimized Nitrogen Topdressing Strategies Enhance Steviol Glycoside Productivity in Stevia (Stevia rebaudiana Bertoni) Plants

2020 ◽  
Vol 20 (3) ◽  
pp. 1133-1143 ◽  
Author(s):  
Yuming Sun ◽  
Yongheng Yang ◽  
Menglan Hou ◽  
Xiaolei Huang ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener

2016 ◽  
Vol 52 (3) ◽  
pp. 330-337 ◽  
Author(s):  
Buhara Yücesan ◽  
Refik Büyükgöçmen ◽  
Aliyu Mohammed ◽  
Muhammad Sameeullah ◽  
Cevher Altuğ ◽  
...  
Keyword(s):  
High Intensity ◽  
Stevia Rebaudiana ◽  
Regeneration System ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Efficient Regeneration

scholarly journals Application of in vitro stevia (Stevia rebaudiana Bertoni) cultures in obtaining steviol glycoside rich material

2015 ◽  
Vol 61 (1) ◽  
pp. 50-63 ◽  
Author(s):  
Aleksandra Luwańska ◽  
Aleksandra Perz ◽  
Grażyna Mańkowska ◽  
Karolina Wielgus
Keyword(s):  
Food Industry ◽  
Stevia Rebaudiana ◽  
Sweet Taste ◽  
Steviol Glycosides ◽  
Practical Applications ◽  
Culture Techniques ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Rich Material

Summary Stevia is a plant attracting attention due to its capability to synthesize a group of chemical compounds with sweet taste, i.e. steviol glycosides. Steviol glycosides are successfully applied as a natural sweetener, and some of them have also therapeutic properties. This paper presents available information on the use of stevia plant tissue cultures with the focus on their potential application in food industry. Detailed analysis was done concerning the research employing in vitro culture techniques and the use of them in biosynthesis of secondary metabolites of high importance for the food industry. Both established achievements and most recent publications on stevia were used for assessment of practical applications of the aforementioned techniques and prospects for their development.

Comparison of different in vitro micropropagation methods of Stevia rebaudiana B. including temporary immersion bioreactor (BIT®)

2017 ◽  
Vol 131 (1) ◽  
pp. 195-199 ◽  
Author(s):  
Karel Vives ◽  
Iván Andújar ◽  
José Carlos Lorenzo ◽  
Oscar Concepción ◽  
Martha Hernández ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
In Vitro Micropropagation
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