Towards a semi-perennial culture of Stevia rebaudiana (Bertoni) Bertoni under temperate climate: effects of genotype, environment and plant age on steviol glycoside content and composition

2015 ◽  
Vol 63 (4) ◽  
pp. 685-694 ◽  
Author(s):  
Claire Barbet-Massin ◽  
Simon Giuliano ◽  
Lionel Alletto ◽  
Jean Daydé ◽  
Monique Berger
Keyword(s):  
Stevia Rebaudiana ◽  
Plant Age ◽  
Temperate Climate ◽  
Climate Effects ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

Salt-tolerance mechanisms induced in Stevia rebaudiana Bertoni: Effects on mineral nutrition, antioxidative metabolism and steviol glycoside content

2017 ◽  
Vol 115 ◽  
pp. 484-496 ◽  
Author(s):  
Daniel Cantabella ◽  
Abel Piqueras ◽  
José Ramón Acosta-Motos ◽  
Agustina Bernal-Vicente ◽  
José A. Hernández ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Mineral Nutrition ◽  
Stevia Rebaudiana ◽  
Tolerance Mechanisms ◽  
Antioxidative Metabolism ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

scholarly journals Pertumbuhan, Produksi Biomassa, dan Kandungan Glikosida Steviol pada Lima Klon Stevia Introduksi di Bogor, Indonesia

2019 ◽  
Vol 47 (1) ◽  
pp. 105-110
Author(s):  
Masna Maya Sinta ◽  
Dan Sumaryono
Keyword(s):  
High Performance ◽  
Biomass Yield ◽  
Stevia Rebaudiana ◽  
Growth And Yield ◽  
Plant Canopy ◽  
Rebaudioside A ◽  
Plant Mortality ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Leaf Yield

Stevia rebaudiana Bertoni, a sweetener plant, has been cultivated in Indonesia since the early 1980s, but the yield is lower than in other countries. Five selected introduced stevia clones were planted at Megamendung, Bogor, Indonesia (6°39’ S, 106°56’ E, 800 m above sea level) on December 2014 until November 2015 to evaluate their growth and biomass yield. The growth and yield were observed from 6 to 12 months after planting. In addition, stevioside, rebaudioside A (reb A), and total steviol glycoside (TSG) contents of leaves were measured using high performance liquid chromatography (HPLC). At six months after planting, stem diameter was 8-11 mm, plant canopy diameter was 23-30 cm, plant height was 24-35 cm, and plant mortality was less than 5% except for clone BU at 30.7% due to Sclerotium attack. The plants flowered at different times, therefore had different harvest durations, from 4 weeks (clone BM) to 8 weeks (clone BP). The highest leaf yield was clone BM (6.04 ton ha-1 per year), followed by BX (4.91 ton ha-1 per year), and BP (4.38 ton ha-1 per year). From the five clones tested, clone BM was the best for leaf, TSG, and stevioside yields; whereas clone BS and BP were the best for reb A yield. Keywords: rebaudioside A, Stevia rebaudiana, stevioside, total steviol glycoside

Optimized Nitrogen Topdressing Strategies Enhance Steviol Glycoside Productivity in Stevia (Stevia rebaudiana Bertoni) Plants

2020 ◽  
Vol 20 (3) ◽  
pp. 1133-1143 ◽  
Author(s):  
Yuming Sun ◽  
Yongheng Yang ◽  
Menglan Hou ◽  
Xiaolei Huang ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener

2016 ◽  
Vol 52 (3) ◽  
pp. 330-337 ◽  
Author(s):  
Buhara Yücesan ◽  
Refik Büyükgöçmen ◽  
Aliyu Mohammed ◽  
Muhammad Sameeullah ◽  
Cevher Altuğ ◽  
...  
Keyword(s):  
High Intensity ◽  
Stevia Rebaudiana ◽  
Regeneration System ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Efficient Regeneration

scholarly journals Trichoderma Asperellum, an inoculant for production of steviol glycoside in Stevia Rebaudiana bertoni plants micropropagated in a temporary immersion bioreactor

2020 ◽  
Vol 19 (3) ◽  
pp. 1153-1161 ◽  
Author(s):  
"D. " Villamarín-Gallegos ◽  
◽  
"D. G. " Oviedo-Pereira ◽  
"S. " Evangelista-Lozano ◽  
"G. " Sepúlveda-Jiménez ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Trichoderma Asperellum ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni

scholarly journals Application of in vitro stevia (Stevia rebaudiana Bertoni) cultures in obtaining steviol glycoside rich material

2015 ◽  
Vol 61 (1) ◽  
pp. 50-63 ◽  
Author(s):  
Aleksandra Luwańska ◽  
Aleksandra Perz ◽  
Grażyna Mańkowska ◽  
Karolina Wielgus
Keyword(s):  
Food Industry ◽  
Stevia Rebaudiana ◽  
Sweet Taste ◽  
Steviol Glycosides ◽  
Practical Applications ◽  
Culture Techniques ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Rich Material

Summary Stevia is a plant attracting attention due to its capability to synthesize a group of chemical compounds with sweet taste, i.e. steviol glycosides. Steviol glycosides are successfully applied as a natural sweetener, and some of them have also therapeutic properties. This paper presents available information on the use of stevia plant tissue cultures with the focus on their potential application in food industry. Detailed analysis was done concerning the research employing in vitro culture techniques and the use of them in biosynthesis of secondary metabolites of high importance for the food industry. Both established achievements and most recent publications on stevia were used for assessment of practical applications of the aforementioned techniques and prospects for their development.

scholarly journals Microwave-Assisted Subcritical Water Extraction of Steviol Glycosides From Stevia rebaudiana Leaves

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1986000 ◽  
Author(s):  
Zheng Yang ◽  
Brandon Uhler ◽  
Tristan Lipkie
Keyword(s):  
Subcritical Water ◽  
Stevia Rebaudiana ◽  
Cost Effective ◽  
Extraction Methods ◽  
Water Extraction ◽  
Steviol Glycosides ◽  
Subcritical Water Extraction ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Green Approach

A rapid method that combines microwave heating and subcritical water extraction to extract steviol glycosides from Stevia rebaudiana (Bertoni) or stevia leaves was developed and compared to conventional thermal solvent extraction methods. Within only 1 minute after subcritical water condition at 140°C being reached, the yields of major steviol glycoside, rebaudioside A and stevioside, and less polar steviol glucoside, rebaudioside C, from stevia leaves were comparable to conventional extraction method that used 70% ethanol under sonication for 45 minutes. This new hybrid extraction technique may provide a cost-effective and green approach for commercial production of high-purity steviol glycoside sweeteners and their “organic” versions without the use of highly expensive “organic” ethanol.

scholarly journals Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener

Biomolecules ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Zheng Yang ◽  
Brandon Uhler ◽  
Ted Zheng ◽  
Kristie Adams
Keyword(s):  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Market Place ◽  
Solubility In Water ◽  
The Third ◽  
Steviol Glycoside ◽  
Stevia Rebaudiana Bertoni ◽  
Enhanced Solubility ◽  
Food And Beverage

Zero-calorie high-intensity sweeteners from natural sources perform very well in the market place. This has encouraged food scientists to continue the effort to search for novel natural ingredients to satisfy consumer demand. Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A). Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill’s propriety enzyme and sucrose as a glucose donor. A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy. Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.

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