KETERKAITAN ANTIGEN NS1 INFEKSI VIRUS DENGUE DENGAN SEROTIPE VIRUS DENGUE

Dengue virus infection (DVI) currently is detected by using dengue virus NS1 antigen (NS1 Ag). The sensitivity of NS1 Ag is 27.8%–93.4%,but recent study of Kumarasamy the sensitivity of NS1 Ag is better than the virus isolation and polymerase chain reaction (PCR). This studyis focussed on the evaluation of the validity of Panbio Dengue Early Rapid for the diagnosis of DVI and the NS1 Ag sensitivity associated withdengue virus serotypes. The sera was obtained from 65 DVI patients which diagnosed by the clinicians. The resulted diagnosis was foundby serology tests (positive IgM/IgG antidengue/NS1 Ag ELISA) and 1997 WHO criteria as the gold standard, and which also found 35 nonDVI patients (typhoid fever, HAV, malaria, UTI, tuberculosis and bronchopneumonia). The samples were examined by Panbio Dengue EarlyRapid. PCR was performed on each positive serological test result to determine the dengue virus serotypes. The sensitivity and specificity ofPanbio Dengue Early Rapid was 49.2% and 100%. The PCR results of 65 sera showed positive PCR in 49.2% (positive NS1 Ag was 62.5%).Meanwhile, and negative PCR in 50.8% (positive NS1 Ag was 36.4%). The predominance of serotypes (positive NS1 Ag) were DEN-3 (37.5%),DEN-4 (28.1%), DEN-1 (21.9%) and DEN-2 (12.5%). The Panbio Dengue Early Rapid can be used as early detection of DVI, although itshould be used in conjunction with other dengue serological tests as well. Unfortunately there is still not enough evidence about the NS1 Agsensitivity associated with the dengue virus serotypes.

Download Full-text

IDENTIFICATION OF DENGUE VIRUS SEROTYPES AT THE DR. SOETOMO HOSPITAL SURABAYA IN 2016 AND ITS CORRELATION WITH NS1 ANTIGEN DETECTION

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v23i2.1138 ◽

2018 ◽

Vol 23 (2) ◽

pp. 151

Author(s):

Jeine Stela Akualing ◽

Aryati Aryati ◽

Puspa Wardhani ◽

Usman Hadi

Keyword(s):

Polymerase Chain Reaction ◽

Dengue Virus ◽

Reverse Transcriptase ◽

Ribonucleic Acid ◽

Rna Virus ◽

Rt Pcr ◽

Chain Reaction ◽

Ns1 Antigen ◽

Dengue Virus Serotypes ◽

Polymerase Chain

Serotipe virus dengue yang beredar terus mengalami perubahan dan berbeda di setiap daerah. Pergeseran serotipe maupun genotipedi dalamnya, mempengaruhi terjadinya wabah dengue di berbagai negara. Perbedaan serotipe diduga bernasab dengan deteksi antigen(Ag) non-structural 1 (NS1), namun belum banyak penelitian yang mendukung hal tersebut. Penelitian potong lintang dikerjakan sejakFebruari-Agustus 2016 dan didapatkan 60 subjek infeksi virus dengue (IVD) dan 25 non-IVD. Ribonucleic acid (RNA) virus denguediperiksa di semua subjek menggunakan Simplexa Dengue Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)termasuk identifikasi serotipe virus dengue dan pemeriksaan NS1 menggunakan uji cepat NS1 Panbio. Perbedaan perbandingan variabelkategorikal dianalisis dengan uji Fisher Exact. Kenasaban antara serotipe dengan deteksi Ag NS1 dianalisis dengan Chi-Kuadrat. RNAvirus dengue terdeteksi di 43 dari 60 subjek IVD (71,7%). Serotipe terbanyak adalah DENV-3 (62,8%). Pergeseran dominasi serotipetelah terjadi di Surabaya, sebelumnya dari DENV-2 ke DENV-1 dan sekarang DENV-3, kemungkinan akibat mobilitas pejamu, transporvirus dan faktor geografis. Kepekaan uji cepat NS1 75% dan kekhasan 100%. Persentase deteksi NS1 antar serotipe berbeda bermakna(p=0,002). Deteksi NS1 lebih rendah pada DENV-1 dibandingkan DENV-2 (p=0,007) ataupun DENV-3 (p=0,003). Serotipe virusdengue bernasab dengan deteksi NS1 (p=0,005). Ciri serotipe maupun genotipe virus dengue kemungkinan mempengaruhi sekresiNS1. Telah terjadi pergeseran serotipe virus dengue di pasien IVD di Surabaya sehingga diperlukan surveillance berkesinambunganuntuk memperkirakan terjadinya wabah. Serotipe bernasab dengan deteksi NS1. Salah satu penyebab hasil negatif palsu NS1 adalahperbedaan serotipe.

Download Full-text

Faculty Opinions recommendation of Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1054775.506701 ◽

2006 ◽

Author(s):

Scott Halstead

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Laboratory Diagnosis ◽

Dengue Virus Infection ◽

Capture Elisa ◽

Antigen Capture Elisa ◽

Antigen Capture ◽

Ns1 Antigen

Download Full-text

Evaluation of Two New Commercial Tests for the Diagnosis of Acute Dengue Virus Infection Using NS1 Antigen Detection in Human Serum

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0000280 ◽

2008 ◽

Vol 2 (8) ◽

pp. e280 ◽

Cited By ~ 119

Author(s):

Philippe Dussart ◽

Laure Petit ◽

Bhety Labeau ◽

Laetitia Bremand ◽

Alexandre Leduc ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Human Serum ◽

Antigen Detection ◽

Dengue Virus Infection ◽

Ns1 Antigen

Download Full-text

Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

Clinical Infectious Diseases ◽

10.1093/cid/cix672 ◽

2017 ◽

Vol 65 (11) ◽

pp. 1829-1836 ◽

Cited By ~ 40

Author(s):

Wen-Yang Tsai ◽

Han Ha Youn ◽

Carlos Brites ◽

Jih-Jin Tsai ◽

Jasmine Tyson ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Zika Virus ◽

Dengue Virus Infection ◽

Serological Tests ◽

Zika Virus Infection

Download Full-text

NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v17i4.38334 ◽

2018 ◽

Vol 17 (4) ◽

pp. 669-673

Author(s):

Mahmuda Siddiqua ◽

Ahmed Nawsher Alam ◽

AKM Muraduzzaman ◽

Tahmina Shirin

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Infection ◽

Medical Science ◽

Cost Effective ◽

Rt Pcr ◽

Dengue Virus Infection ◽

Medical College ◽

Virus Serotype ◽

Ns1 Antigen

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

Download Full-text

System Analysis of Dengue Virus Surveillance in BBTKL PP Surabaya Year 2012–2014

Jurnal Berkala Epidemiologi ◽

10.20473/jbe.v3i2.2015.146-157 ◽

2015 ◽

Vol 3 (2) ◽

pp. 146

Author(s):

Atina Husnayain ◽

Kurnia Dwi Artanti ◽

Acub Zaenal

Keyword(s):

Dengue Virus ◽

Data Storage ◽

System Analysis ◽

Alternative Solution ◽

Problem Analysis ◽

Chain Reaction ◽

Virus Surveillance ◽

Dengue Virus Serotypes ◽

Polymerase Chain

ABSTRACTChanging the distribution of dengue virus serotypes has occurred in Indonesia. This condition should be monitored continuously through the Dengue Virus Surveillance. Implementation of Dengue Virus Surveillance also conducted by BBTKL PP Surabaya. The purpose of this study was to determine the workflow, identify problems, set priority problem, find the cause of the problem, and provide the alternative solution related to problems of Dengue Virus Surveillance in BBTKL PP Surabaya. This is a operational research and the informants are officers of Dengue Virus Surveillance in BBTKL PP Surabaya. Data in this study was analyzed descriptive and presented narrative. Results showed that the workflow of Dengue Virus Surveillance in BBTKL PP Surabaya are collecting of patient’s blood and vector specimen, vector survey and collecting the supporting data, Rapid Diagnostic Test (RDT) and Polymerase Chain Reaction (PCR), processing and data analysis, and dissemination the information. The main problems of Dengue Virus Surveillance in BBTKL PP Surabaya is the low quality of the information. Tree problem analysis showed that the cause of problem that can be intervene are incomplete supporting data and data storage. Alternative solution related to problems of Dengue Virus Surveillance in BBTKL PP Surabaya is use of Epi Info.Keyword: surveillance, dengue virus, data management

Download Full-text

A Study on the Prevalence of Dengue Virus Infection using NS1 Antigen and IgM Antibody capture ELISA for the Early Diagnosis in and around Madurantakam, India

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2019.802.187 ◽

2019 ◽

Vol 8 (02) ◽

pp. 1596-1600

Author(s):

R. Ganesan ◽

T. Sheila Doris Devamani ◽

D. Joseph Pushpa Innocent

Keyword(s):

Virus Infection ◽

Early Diagnosis ◽

Dengue Virus ◽

Dengue Virus Infection ◽

Capture Elisa ◽

Igm Antibody ◽

Ns1 Antigen ◽

Antibody Capture

Download Full-text

Clinical evaluation of the NS1 antigen-capture ELISA for early diagnosis of dengue virus infection in Brazil

Journal of Medical Virology ◽

10.1002/jmv.21814 ◽

2010 ◽

Vol 82 (8) ◽

pp. 1400-1405 ◽

Cited By ~ 23

Author(s):

Luiza A. Castro-Jorge ◽

Paula R.L. Machado ◽

Camila A. Fávero ◽

Marcos C. Borges ◽

Luzia M.R. Passos ◽

...

Keyword(s):

Virus Infection ◽

Early Diagnosis ◽

Dengue Virus ◽

Clinical Evaluation ◽

Dengue Virus Infection ◽

Capture Elisa ◽

Antigen Capture Elisa ◽

Antigen Capture ◽

Ns1 Antigen

Download Full-text

A Pan-Dengue Virus Reverse Transcription-Insulated Isothermal PCR Assay Intended for Point-of-Need Diagnosis of Dengue Virus Infection by Use of the POCKIT Nucleic Acid Analyzer

Journal of Clinical Microbiology ◽

10.1128/jcm.00225-16 ◽

2016 ◽

Vol 54 (6) ◽

pp. 1528-1535 ◽

Cited By ~ 21

Author(s):

Yun Young Go ◽

R. P. V. Jayanthe Rajapakse ◽

Senanayake A. M. Kularatne ◽

Pei-Yu Alison Lee ◽

Keun Bon Ku ◽

...

Keyword(s):

Nucleic Acid ◽

Virus Infection ◽

Dengue Virus ◽

Rapid Test ◽

Diagnostic Assay ◽

Pcr Assay ◽

Dengue Virus Infection ◽

Qrt Pcr ◽

Ns1 Antigen ◽

Insulated Isothermal Pcr

Dengue virus (DENV) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. Here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal PCR (RT-iiPCR) method for the detection of all four serotypes of DENV in clinical samples. The diagnostic performance of the newly established pan-DENV RT-iiPCR assay targeting a conserved 3′ untranslated region of the viral genome was evaluated. The limit of detection with a 95% confidence was estimated to be 10 copies ofin vitro-transcribed (IVT) RNA. Sensitivity analysis using RNA prepared from 10-fold serial dilutions of tissue culture fluid containing DENVs suggested that the RT-iiPCR assay was comparable to the multiplex real-time quantitative RT-PCR (qRT-PCR) assay for DENV-1, -3, and -4 detection but 10-fold less sensitive for DENV-2 detection. Subsequently, plasma collected from patients suspected of dengue virus infection (n= 220) and individuals not suspected of dengue virus infection (n= 45) were tested by the RT-iiPCR and compared to original test results using a DENV NS1 antigen rapid test and the qRT-PCR. The diagnostic agreement of the pan-DENV RT-iiPCR, NS1 antigen rapid test, and qRT-PCR tests was 93.9%, 84.5%, and 97.4%, respectively, compared to the composite reference results. This new RT-iiPCR assay along with the portable POCKIT nucleic acid analyzer could provide a highly reliable, sensitive, and specific point-of-need diagnostic assay for the diagnosis of DENV in clinics and hospitals in developing countries.

Download Full-text