scholarly journals PENILAIAN UJI TROPONIN I DENGAN POINT OF CARE TESTING

2018 ◽  
Vol 22 (2) ◽  
pp. 114
Author(s):  
Sheila Febriana ◽  
Asvin Nurulita ◽  
Uleng Bahrun
Keyword(s):  
Whole Blood ◽  
Clinical Condition ◽  
Troponin I ◽  
Task Force ◽  
Point Of Care ◽  
Pearson Correlation ◽  
World Health ◽  
P Value ◽  
Point Of Care Testing ◽  
The Mean

Troponin I is a cardiac biomarker recomended by The Third Global Myocardial Infarction Task Force World Health Organisation(WHO). Troponin plays a central role as a relevant biomarker that require reliable samples, methods, device and efficiency of time.Selecting the device, methods and sample used in the assay may affect the results and turn arround time. The aim of this study is toknow troponin I result using Point of care Testing device with a flourescence immunoassay methods using whole blood and laboratorybasedanalysis device with Enzyme-Linked Fluorescent Assay (ELFA) methods using serum by evaluation. Cross sectional study was heldon 50 subjects in Wahidin Sudirohusodo hospital during the period between July-August 2015, those who suspected suffering acutecoronary syndome (ACS) and underwent troponin I test ordered by the physician and also had whole blood sample. The subjects arearound 51.96±12.80 year old and most of them are men (62%). The mean consentration of troponin I with laboratoric-based analysis is0.50±1.69 μg/L and with POCT is 0.51±1.77. The Pearson correlation test shows the correlation (r) is 0.99 with the p value is <0.001.Bland and Altman methods show the mean difference between two assays is 0.014μg/L (95% confidence interval, -0.015; 0.043) withthe limit of agreement -0.19 to 0.22. Based on this study, it can be concluded that troponin I assay using POCT device can be used tosupport ACS diagnosis precisely and rapidly. It is suggested to perform futher study with concern on the patient’s clinical condition aswell as the diagnosis, so it can evaluate the device performance to measure troponin I levels consistently with the clinical condition.

scholarly journals Clinical Evaluation of the First Medical Whole Blood, Point-of-Care Testing Device for Detection of Myocardial Infarction

2000 ◽  
Vol 46 (10) ◽  
pp. 1604-1609 ◽  
Author(s):  
Fred S Apple ◽  
F Philip Anderson ◽  
Paul Collinson ◽  
Robert L Jesse ◽  
Michael C Kontos ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Whole Blood ◽  
Troponin I ◽  
Point Of Care ◽  
Point Of Care Testing ◽  
Test Device ◽  
Cardiac Marker ◽  
St Segment Elevation ◽  
St Segment ◽  
Diagnostic Sensitivity And Specificity

Abstract Background: Validation of whole blood, point-of-care testing devices for monitoring cardiac markers to aid clinicians in ruling in and ruling out myocardial infarction (MI) is necessary for both laboratory and clinical acceptance. Methods: This study evaluated the clinical diagnostic sensitivity and specificity of the First Medical Cardiac Test device operated by nursing and laboratory personnel that simultaneously measures cardiac troponin I (cTnI), creatine kinase (CK) MB, myoglobin, and total CK on the Alpha Dx analyzer in whole blood for detection of MI. Over a 6-month period, 369 patients initially presenting to the emergency department with chest pain were evaluated for MI using modified WHO criteria. Eighty-nine patients (24%) were diagnosed with MI. Results: In whole blood samples collected at admission and at 3- to 6-h intervals over 24 h, ROC curve-determined MI decision limits were as follows: cTnI, 0.4 μg/L; CKMB, 7.0 μg/L; myoglobin, 180 μg/L; total CK, 190 μg/L. Based on peak concentrations within 24 h after presentation, the following sensitivities (± 95% confidence intervals) were found: cTnI, 93% ± 5.5%; myoglobin, 81% ± 9.7%; CKMB, 90% ± 6.3%; total CK, 86% ± 7.5%. Sensitivities were maximal at &gt;90% for both cTnI and CKMB at &gt;12 h in MI patients, without differences between ST-segment elevation and non-ST-segment elevation MI patients. Conclusions: The First Medical point-of-care device provides cardiac marker assays that can be used by laboratories and clinicians in a variety of hospital settings for ruling in and ruling out MI.

Point-of-Care Testing Using Invasive and Non-Invasive Hemoglobinometers: Reliable and Valid Method for Estimation of Hemoglobin among Children 6–59 Months

2020 ◽  
Author(s):  
Gomathi Ramaswamy ◽  
Kashish Vohra ◽  
Kapil Yadav ◽  
Ravneet Kaur ◽  
Tripti Rai ◽  
...  
Keyword(s):  
Point Of Care ◽  
Venous Blood ◽  
Public Health Problem ◽  
Outpatient Department ◽  
Point Of Care Testing ◽  
Adequate Treatment ◽  
Non Invasive ◽  
Hematology Analyzer ◽  
The Mean ◽  
Study Participants

Abstract Introduction Globally around 47.4% of children and in India, 58% of children aged 6–59 months are anemic. Diagnosis of anemia in children using accurate technologies and providing adequate treatment is essential to reduce the burden of anemia. Point-of-care testing (POCT) devices is a potential option for estimation of hemoglobin in peripheral and field settings were the hematology analyzer and laboratory services are not available. Objectives To access the validity of the POCTs (invasive and non-invasive devices) for estimation of hemoglobin among children aged 6–59 months compared with hematology analyzer. Methods The study participants were enrolled from the pediatric outpatient department in Haryana, India, from November 2019 to January 2020. Hemoglobin levels of the study participants were estimated in Sahli’s hemoglobinometer and invasive digital hemoglobinometers (DHs) using capillary blood samples. Hemoglobin levels in non-invasive DH were assessed from the finger/toe of the children. Hemoglobin levels measured in POCTs were compared against the venous blood hemoglobin estimated in the hematology analyzer. Results A total of 120 children were enrolled. The mean (SD) of hemoglobin (g/dl) estimated in auto-analyzer was 9.4 (1.8), Sahli’s hemoglobinometer was 9.2 (1.9), invasive DH was 9.7 (1.9), and non-invasive DH was 11.9 (1.5). Sahli’s hemoglobinometer (95.5%) and invasive DH (92.2%) had high sensitivity for the diagnosis of anemia compared with non-invasive DH (24.4%). In contrast, non-invasive DH had higher specificity (96.7%) compared with invasive DH (83.3%) and Sahli’s hemoglobinometer (70%). Invasive DH took the least time (2–3 min) for estimation of hemoglobin per participant, followed by Sahli’s (4–5 min) and non-invasive DH (5–7 min). Conclusion All three POCT devices used in this study are reasonable and feasible for estimating hemoglobin in under-5 children. Invasive DHs are potential POCT devices for diagnosis of anemia among under-5 children, while Sahli’s can be considered as a possible option, where trained and skilled technicians are available. Further research and development are required in non-invasive DH to improve accuracy. Lay summary In India, anemia is a serious public health problem, where 58% of the children aged 6–59 months are anemic. Point-of-care testing (POCT) using digital hemoglobinometers (DHs) has been recommended as one of the key interventions by the Anemia Mukt Bharat program since 2018 in India. These POCT devices are easy to use, less invasive, can be carried to field, require minimal training and results are available immediately. Therefore this study assessed the validity of POCT devices—invasive DH, non-invasive DH and Sahli’s hemoglobinometer among 6–59 months children in facility setting compared with the gold standard hematology analyzer. A total of 120 children under 6–59 months of age were enrolled from the pediatric outpatient department in Haryana, India, from November 2019 to January 2020. The (mean hemoglobin in g/dl) invasive (9.7) and non-invasive DH (11.9) overestimated hemoglobin value, while Sahli’s (9.2) underestimated hemoglobin compared with hematology analyzer (9.4). Invasive DH (92.2%) and Sahli’s hemoglobinometer (95.5%) reported high ability to correctly identify those with anemia compared with non-invasive DH (24.4%). In contrast, non-invasive DH (96.73%) had higher ability to correctly identify those without the anemia compared with invasive DH (83.3%) and Sahli’s (70%).

Effect of Troponin I Point-of-Care Testing on Emergency Department Throughput Measures and Staff Satisfaction

2013 ◽  
Vol 35 (3) ◽  
pp. 270-277 ◽  
Author(s):  
Jeanniline Koehler ◽  
Kathleen Flarity ◽  
George Hertner ◽  
Judy Aker ◽  
John Patrick Stout ◽  
...  
Keyword(s):  
Emergency Department ◽  
Troponin I ◽  
Point Of Care ◽  
Staff Satisfaction ◽  
Point Of Care Testing

scholarly journals Food variety and dietary diversity scores in children: are they good indicators of dietary adequacy?

2006 ◽  
Vol 9 (5) ◽  
pp. 644-650 ◽  
Author(s):  
NP Steyn ◽  
JH Nel ◽  
G Nantel ◽  
G Kennedy ◽  
D Labadarios
Keyword(s):  
Sensitivity And Specificity ◽  
Dietary Diversity ◽  
Pearson Correlation ◽  
Child Growth ◽  
World Health ◽  
Food Groups ◽  
Nutrient Adequacy ◽  
Z Scores ◽  
Food Variety ◽  
The Mean

AbstractObjectiveTo assess whether a food variety score (FVS) and/or a dietary diversity score (DDS) are good indicators of nutrient adequacy of the diet of South African children.MethodsSecondary data analyses were undertaken with nationally representative data of 1–8-year-old children (n = 2200) studied in the National Food Consumption Study in 1999. An average FVS (mean number of different food items consumed from all possible items eaten) and DDS (mean number of food groups out of nine possible groups) were calculated. A nutrient adequacy ratio (NAR) is the ratio of a subject's nutrient intake to the estimated average requirement calculated using the Food and Agriculture Organization/World Health Organization (2002) recommended nutrient intakes for children. The mean adequacy ratio (MAR) was calculated as the sum of NARs for all evaluated nutrients divided by the number of nutrients evaluated, expressed as a percentage. MAR was used as a composite indicator for micronutrient adequacy. Pearson correlation coefficients between FVS, DDS and MAR were calculated and also evaluated for sensitivity and specificity, with MAR taken as the ideal standard of adequate intake. The relationships between MAR and DDS and between anthropometric Z-scores and DDS were also evaluated.ResultsThe children had a mean FVS of 5.5 (standard deviation (SD) 2.5) and a mean DDS of 3.6 (SD 1.4). The mean MAR (ideal = 100%) was 50%, and was lowest (45%) in the 7–8-year-old group. The items with the highest frequency of consumption were from the cereal, roots and tuber group (99.6%), followed by the ‘other group’ (87.6%) comprising items such as tea, sugar, jam and sweets. The dairy group was consumed by 55.8%, meat group by 54.1%, fats by 38.9%, other vegetables by 30.8%, vitamin-A-rich by 23.8%, other fruit by 22%, legumes and nuts by 19.7% and eggs by 13.3%. There was a high correlation between MAR and both FVS (r = 0.726; P < 0.0001) and DDS (r = 0.657; P < 0.0001), indicating that either FVS or DDS can be used as an indicator of the micronutrient adequacy of the diet. Furthermore, MAR, DDS and FVS showed significant correlations with height-for-age and weight-for-age Z-scores, indicating a strong relationship between dietary diversity and indicators of child growth. A DDS of 4 and an FVS of 6 were shown to be the best indicators of MAR less than 50%, since they provided the best sensitivity and specificity.ConclusionEither FVS or DDS can be used as a simple and quick indicator of the micronutrient adequacy of the diet.

Development and Validation of a GC-MS Method for the Quantitation of Nanoformulated Primaquine in Whole Blood and Plasma of Mouse Model

2017 ◽  
Vol 2 (1) ◽  
pp. 44-58
Author(s):  
James Jorum Owuor ◽  
Florence Oloo ◽  
Martin Ongas ◽  
Caroline Kirimi ◽  
Wesley Nyaigoti Omwoyo ◽  
...  
Keyword(s):  
Whole Blood ◽  
Internal Standard ◽  
Small Sample ◽  
Precipitation Method ◽  
Gas Chromatography Mass Spectrometry ◽  
P Value ◽  
Blood Collection ◽  
Significant Difference ◽  
Product Ions ◽  
The Mean

A Gas chromatography-mass spectrometry (GC/MS) method was developed and validated for the quantitation of the antimalarial drug, nanoformulated Primaquine (PQ), in whole blood and plasma. The analyte was extracted using a protein precipitation method followed by chromatographic separation on a Waters Xterra, RP C8, 2.5µm, 50mm x 4.6mm analytical column with a mobile phase consisting of A: 0.5% Formic acid in 20mM NH4COOH, B: Methanol pH adjusted to 3.0 with FA at a ratio of 3:7 (v/v), delivered at a constant flow rate of 0.5 ml/min. Mefloquine (MEF) was used as the internal standard. Compound reaction monitoring was performed using 260.4 Da for precursor ion and 175. 2 and 379.2 Da for product ions for the quantification of PQ and 379.2 Da for precursor ion and 175.2 and 379.2 Da for product ions for the quantification, respectively. Calibration curves were constructed over the concentration range 16.7–4300 ng/ml. The mean intra- and inter-assay accuracy values for the analysis of PQ in WB was 104% (%CV = 5.6) and 98.6% (%CV = 5.7), respectively. The mean intra- and inter-assay accuracy values for the analysis of PQ in plasma was 92.7% (%CV = 3.7) and 93.7% (%CV = 5.4), respectively. No significant matrix effect was observed during the method validation. The validated method was applied to an absorption study in mice, to determine and compare PQ concentrations in whole blood and plasma samples. Results of the statistical analysis using a linear mixed effects growth curve model concluded that there was no significant difference (p-value = 0.688) between WB and plasma PQ concentrations. This method utilizes a small sample volume of 20 µl, facilitating low blood collection volumes and a short chromatographic run time of 3 min which allows for high sample through put analysis.

scholarly journals 375. Cryptococcal Antigenemia in Advanced HIV Infection

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S196-S196
Author(s):  
Jatin Ahuja ◽  
Manish Soneja ◽  
Naveet Wig ◽  
Immaculata Xess ◽  
Ashutosh Biswas ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Care Center ◽  
Tertiary Care ◽  
Latex Agglutination ◽  
Cd4 Count ◽  
North India ◽  
World Health ◽  
P Value ◽  
The Mean

Abstract Background Diagnostic importance of asymptomatic cryptococcal antigenemia is being increasingly recognized in the last few years. Recently, WHO (World Health Organization) has recommended routine screening of CrAg (cryptococcal antigen) among PLHA with CD4 ≤100/mm3, albeit this procedure is not yet adopted by many developing countries including India. Methods We conducted a prospective observational study in a large tertiary care center of North India, upon ethical clearance. Latex agglutination test was performed to assess serum CrAg levels, followed by the lumbar puncture for detection of CrAg levels in the CSF. We analyzed the prevalence and treatment outcomes of cryptococcal antigenemia among PLHA with CD4 ≤ 100 cells/mm3. Detailed clinical examination was conducted, with follow-up of upto 3 months. Multivariate analysis was performed for the estimation of risk factors. Results The mean age (years) and BMI (kg/m2) of all the participants were 41.4 ± 11.2 and 22.1 ± 2.6, respectively. Notably, the mean CD4 count (cu.mm) at the time of recruitment was 62.3 ± 20.5. Noteworthy, 62 (60.8%) of the patients were ART naïve. We found 9.8% (n = 10) of the patients were positive for serum CrAg, and only 2.9% (n = 3) had clinical features of meningitis and 6.8% (n = 7) were asymptomatic (subclinical) CrAg positive. Strikingly, 3.9% (n = 4) of the asymptomatic cryptococcal antigenemia patients were also positive for CrAg in CSF, with 1.9% (n = 2) were only serum CrAg positive, and 1 patient was lost to follow-up (Graph 1). Multivariate analysis revealed that patients with long duration of HIV (P = 0.04), headache symptoms (P = 0.004) and possessing features of meningismus (P value=0.08) are more likely to be CrAg positive. Conversely, patients on fluconazole were protective against cryptococcal antigenemia (P = 0.1) as shown in Table 1. Overall mortality observed was 11.3% among advanced HIV patients. Moreover, mortality in CrAg-positive patients was 33.3% in comparison to CrAg-negative patients who had 9% (P = 0.06) in 3-months follow-up. Conclusion Cryptococcal antigenemia is common (9.8%) among patients with CD4 count ≤100/mm3 in India. Screening for CrAg should be made routine for PLHA with CD4 count ≤100/mm3 and if required preemptive treatment to be given in this regard. Disclosures All authors: No reported disclosures.

pH Readout enhanced ELISA for point-of-care testing of cardiac troponin I

2017 ◽  
Vol 28 (9) ◽  
pp. 1878-1880 ◽  
Author(s):  
Luyang Miao ◽  
Lianhua Zhang ◽  
Lei Jiao ◽  
Xiaofeng Tan ◽  
Qin Wei ◽  
...  
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Point Of Care ◽  
Cardiac Troponin I ◽  
Point Of Care Testing

scholarly journals RAMPTM: A Rapid, Quantitative Whole Blood Immunochromatographic Platform for Point-of-Care Testing

1999 ◽  
Vol 45 (9) ◽  
pp. 1676-1678 ◽  
Author(s):  
Donald E Brooks ◽  
Dana V Devine ◽  
Paul C Harris ◽  
Joanne E Harris ◽  
Mark E Miller ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Point Of Care Testing

scholarly journals Lung ultrasound patterns in pediatric pneumonia in Mozambique and Pakistan

2020 ◽  
pp. 00518-2020
Author(s):  
Amy Sarah Ginsburg ◽  
Pio Vitorino ◽  
Zunera Qasim ◽  
Jennifer L. Lenahan ◽  
Jun Hwang ◽  
...  
Keyword(s):  
Interrater Reliability ◽  
Lung Ultrasound ◽  
Point Of Care ◽  
Integrated Management ◽  
Secondary Analysis ◽  
World Health ◽  
P Value ◽  
Pediatric Pneumonia ◽  
District Hospitals ◽  
Health Organization

ObjectiveImproved pneumonia diagnostics are needed, particularly in resource-constrained settings. Lung ultrasound (LUS) is a promising point-of-care imaging technology for diagnosing pneumonia. The objective was to explore LUS patterns associated with pediatric pneumonia.MethodsWe conducted a prospective, observational study among children aged 2 through 23 months with World Health Organization Integrated Management of Childhood Illness chest-indrawing pneumonia and among children without fast breathing, chest indrawing or fever (no pneumonia cohort) at two district hospitals in Mozambique and Pakistan. We assessed LUS and chest radiograph (CXR) examinations, and viral and bacterial nasopharyngeal carriage, and performed a secondary analysis of LUS patterns.ResultsLUS demonstrated a range of distinctive patterns that differed between children with and without pneumonia and between children in Mozambique versus Pakistan. The presence of LUS consolidation or interstitial patterns was more common in children with chest-indrawing pneumonia than in those without pneumonia. Consolidations were also more common among those with only bacterial but no viral carriage detected (50.0%) than among those with both (13.0%) and those with only virus detected (8.3%;p-value 0.03). LUS showed high interrater reliability among expert LUS interpreters for overall determination of pneumonia (κ=0.915), consolidation (κ=0.915), and interstitial patterns (κ=0.901), but interrater reliability between LUS and CXR for detecting consolidations was poor (κ=0.159, Pakistan) to fair (κ=0.453, Mozambique).DiscussionPattern recognition was discordant between LUS and CXR imaging modalities. Further research is needed to define and standardise LUS patterns associated with pediatric pneumonia and to evaluate the potential value of LUS as a reference standard.

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