scholarly journals Relationships of Selected Endogenous Factors Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

2018 ◽  
Vol 8 ◽  
pp. 1309-1338
Author(s):  
D. K. Isutsa ◽  
R. N. Mayoli ◽  
A. B. Nyende ◽  
C. M. Mweu
Keyword(s):  
Fatty Acids ◽  
Somatic Embryogenesis ◽  
Embryo Development ◽  
Direct Somatic Embryogenesis ◽  
Economic Returns ◽  
Endogenous Factors ◽  
The Status ◽  
Biochemical Components ◽  
Set Up ◽  
Endogenous Substances

Coffee is one of the most important cash crops produced in the world with great economic returns to growers and national gross domestic product. Somatic embryogenesis is a morphogenetic processes leading to plantlet regeneration and these processes are coupled with changes in the levels of primary metabolites. The present experiment established relationships of endogenous substances with direct somatic embryogenesis of coffee ‘Ruiru 11’. Laboratory experiments were set up at Coffee Research Institute, Ruiru-Kenya between 2014 and 2017. The set up was in a completely randomised design, replicated three times and repeated once. Third leaf pair explants were excised from 8-month-old greenhouse-grown mother plants and cultured in half strength Murashige and Skoog basal salts augmented with Thidiazuron. Once embryos had developed, the cultures were analysed for endogenous substances using HPLC and GCMS. Sucrose, phenolics, alkaloids, amino acids, fatty acids and their derivatives correlated positively, whereas fructose and glucose correlated negatively with the other biochemical components. Endogenous sucrose, chlorogenic acid, caffeine amino acid, fatty acids and their derivatives are potential biomarkers for coffee somatic embryogenesis, whereas endogenous fructose and glucose are inhibitors of the same. Further studies regarding the status of the biochemical components, especially in particular stages of embryo development should be conducted to establish treatments that can improve coffee direct somatic embryo development.

scholarly journals Evaluation of Endogenous Phytocomponents and Amino Acids Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

2018 ◽  
Vol 8 ◽  
pp. 1293-1308
Author(s):  
D. K. Isutsa ◽  
R. N. Mayoli ◽  
A. B. Nyende ◽  
C. M. Mweu
Keyword(s):  
Amino Acids ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Embryo Development ◽  
Direct Somatic Embryogenesis ◽  
Embryogenic Cultures ◽  
Mature Embryos ◽  
The Status ◽  
Somatic Embryo Development ◽  
Set Up

Coffee is one of the most important crops cultivated in the world for use in beverages and confectionaries. Embryogenesis is a complex process that begins with a single cell and ends with the formation of mature embryos. Somatic embryo development involves accumulation of complex metabolites and storage reserves. This present experiment identified and quantified endogenous phytocomponents and amino acids present during somatic embryogenesis of ‘Ruiru 11’. Laboratory experiments for this study were set up in the Coffee Research Institute, Kenya at Ruiru. Third leaf pair explants were excised from 8-month-old greenhouse-grown mother plants sterilized and cultured in half strength Murashige and Skoog basal salts augmented with Thidiazuron. Once embryos had developed, the cultures were analysed for phytocomponents using GCMS and HPLC. The results showed that palmitoleic and stearic acids were highest (23.3 µg/g and 69.9 µg/g respectively) in brown embryogenic cultures. Cis 7,8 epoxy-2-methyl octadecane was highest (253 µg/g) in green embryogenic cultures. (Z)-3-Tetradecene was highest (25 µg/g) in brown non-embryogenic cultures. Z, Z-3,13- Octadecedien-1-ol and (Z)-7-Hexadecenal were highest (32.1 µg/g and 70.2 µg/g respectively) in green embryogenic cultures. Alanine content was highest (4.4 µg/g) in embryos of brown cultures. Amino acids, fatty acids and their derivatives are potential biomarkers for embryogenesis. Other phytocomponents should be identified and their role in coffee somatic embryogenesis determined. Further studies regarding the status of the phytocomponents identified in the present study, especially in particular stages of embryo development are needed to propose treatments to improve coffee somatic embryo development.

scholarly journals Evaluation of Endogenous Sugars, Chlorogenic Acid and Caffeine Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

2018 ◽  
Vol 8 ◽  
pp. 1279-1292
Author(s):  
D. K. Isutsa ◽  
R. N. Mayoli ◽  
A. B. Nyende ◽  
C. M. Mweu
Keyword(s):  
Somatic Embryogenesis ◽  
Chlorogenic Acid ◽  
Direct Somatic Embryogenesis ◽  
Embryogenic Cultures ◽  
Biochemical Compounds ◽  
Mother Plants ◽  
Half Strength ◽  
Set Up ◽  
Basal Salts ◽  
Coffea Arabica L

Coffee is one of the most important cash crops cultivated in the world with great economic importance. During the induction of somatic embryogenesis, there are different endogenous compounds involved in the success or failure of the somatic embryogenesis response and these compounds determine the specificity of cellular responses. This present experiment identified and quantified endogenous sugars, chlorogenic acid and caffeine present during somatic embryogenesis of ‘Ruiru 11’. Laboratory experiments were set up at Coffee Research Institute, Ruiru-Kenya between 2014 and 2016. Third leaf pair explants were excised from 8-monthold greenhouse-grown mother plants and cultured in half strength Murashige and Skoog basal salts augmented with Thidiazuron. Once embryos had developed, the cultures were analysed for endogenous sugars, caffeine and chlorogenic acid using HPLC. Generally, green embryogenic cultures contained more and higher quantities of the compounds. Glucose and fructose were highest (38.95 mg/g and 45.43 mg/g respectively) in leaf discs of brown non-embryogenic cultures. Sucrose was highest (62.15 mg/g) in embryos of green embryogenic cultures. Embryos of green embryogenic cultures had the highest chlorogenic acid (5.3 mg/g), whereas caffeine was highest (0.55 mg/g) in embryos of brown embryogenic cultures. Endogenous fructose and glucose inhibited embryogenesis, while sucrose, chlorogenic acids and caffeine promoted embryogenesis and are potential biomarkers for embryogenesis. Other biochemical compounds such as organic acids should be identified and their role in coffee somatic embryogenesis determined.

Somatic embryogenesis in pumpkin (Cucurbita pepo L.): Control of somatic embryo development by nitrogen compounds

2004 ◽  
Vol 161 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Dunja Leljak-Levanić ◽  
Nataša Bauer ◽  
Snježana Mihaljević ◽  
Sibila Jelaska
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Embryo Development ◽  
Cucurbita Pepo ◽  
Nitrogen Compounds ◽  
Somatic Embryo Development

Did Wittgenstein Speak with the Vulgar or Think with the Learned? Or Did He do Both?

Philosophy ◽  
2007 ◽  
Vol 82 (2) ◽  
pp. 213-233
Author(s):  
John W. Cook
Keyword(s):  
Ordinary Language ◽  
Status Quo ◽  
Actual Use ◽  
The Status ◽  
Set Up

Wittgenstein has often been criticized, and even dismissed, for being a patron of ordinary language, a champion of the vernacular, a defender of the status quo. One critic has written: ‘When Wittgenstein set up the actual use of language as a standard, that was equivalent to accepting a certain set up of culture and belief as a standard … It is lucky no such philosophy was thought of until recently or we should still be under the sway of witch doctors …’ In what follows I want to show just how wide of the mark criticisms of this sort are.

scholarly journals Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in CactusCopiapoa tenuissimaRitt. formamostruosa

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Somatic Embryos ◽  
Sucrose Concentration ◽  
Turgor Pressure ◽  
Direct Somatic Embryogenesis ◽  
Fresh Weight ◽  
High Concentration ◽  
Globular Stage ◽  
Indirect Somatic Embryogenesis

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.

PEDIATRICS AND SOCIETY

PEDIATRICS ◽  
1960 ◽  
Vol 25 (1) ◽  
pp. 145-150
Author(s):  
Lytt I. Gardner
Keyword(s):  
Medical Research ◽  
Public Support ◽  
National Institutes Of Health ◽  
International Institute ◽  
Research Activities ◽  
The Status ◽  
Set Up ◽  
Research Facilities ◽  
And Child Health ◽  
Harvard School

THIS is a progress report to the readers of Pediatrics on the status of Senator Lister Hill's "Health for Peace" bill (Senate Joint Resolution 41). This measure, which would have set up an international institute of medical research, passed the Senate May 20, 1959 with flying colors but finally was snagged in a House subcommittee through the summer of 1959. The legislation received resounding approval by the Senate, with a vote of 63 to 17. The bill proposed to organize an Institute of International Medical Research within the framework of the National Institutes of Health. A $50,000,000 annual appropriation was planned. Evidence of widespread public support for the measure was observed in the statements of authorities who spoke at the Senate hearings concerning the bill. It is of interest that no one appeared to testify against S.J. Res. 41 during the 6 days of hearings. At lease two persons participating in the hearings were members of the American Academy of Pediatrics: Dr. Martha M. Eliot, Head, Department of Maternal and Child Health, Harvard School of Public Health and Dr. Sidney Farber (Honorary Associate Member), Professor of Pathology, Harvard Medical School. Many witnesses pointed out the importance of providing support for the research activities and the training of promising scholars in other lands. On this subject, Dr. Farber had this to say: In many countries, such as Italy, the amount of research support available is so small that men of great skill and intellect are compelled to carry on only token research concerning problems which are selected because they do not require manpower, equipment, or modern research facilities.

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