scholarly journals In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on the solid, liquid and double-phase culture systems

2019 ◽  
Vol 18 (4) ◽  
pp. 47-56
Author(s):  
Behzad Kaviani ◽  
Behnaz Bahari
Keyword(s):  
Culture Media ◽  
Ex Vitro Rooting ◽  
Plant Production ◽  
Axillary Shoot ◽  
Ex Vitro ◽  
Node Number ◽  
Double Phase ◽  
Shoot Number ◽  
Solid Liquid

A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.

scholarly journals Ex Vitro Rooting of American Chestnut Improves Acclimatization Survival and Plantlet Quality

2016 ◽  
Vol 34 (3) ◽  
pp. 75-79 ◽  
Author(s):  
Allison D Oakes ◽  
Tyler R. Desmarais ◽  
William A. Powell ◽  
Charles A. Maynard
Keyword(s):  
Tissue Culture ◽  
Keystone Species ◽  
Ex Vitro Rooting ◽  
Plant Production ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Regulatory Review ◽  
Survival Percentage ◽  
Virus Indexing

Tissue culture of plants has many applications, from producing genetically identical horticultural varieties, to production of secondary metabolites, to virus indexing, and most relevantly, developing novel traits by genetic transformation. Using Agrobacterium-mediated transformation on somatic embryos, blight-resistant American chestnuts [Castanea dentata (Marsh.) Borkh.] have been developed as shoot cultures in plant tissue culture. Rooting tissue-cultured shoots and acclimatizing the rooted plantlets are key steps in tree production. In this study, in vitro and ex vitro rooting methods were compared. The ex vitro method resulted in a lower initial rooting percentage but an overall higher survival percentage, resulting in higher potted plant production. The higher survival was likely due to partial acclimatization taking place before the plantlets were transplanted into potting mix. After 8 weeks, plantlets rooted via the ex vitro method were taller, and had more, and larger, leaves than the in vitro-rooted plantlets. These trees are currently in high demand for inoculation studies for federal regulatory review and eventually for restoration of this keystone species to its native habitat.

scholarly journals Micropropagation and Ex Vitro Rooting of Wolfberry

HortScience ◽  
2018 ◽  
Vol 53 (10) ◽  
pp. 1494-1499 ◽  
Author(s):  
Cristian Silvestri ◽  
Gianmarco Sabbatini ◽  
Federico Marangelli ◽  
Eddo Rugini ◽  
Valerio Cristofori
Keyword(s):  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Ex Vitro Rooting ◽  
Axillary Shoot ◽  
Nodal Segment ◽  
Ex Vitro ◽  
Lycium Barbarum ◽  
Axillary Shoot Proliferation ◽  
Very High

An accurate protocol for the in vitro propagation of a commercial wolfberry (Lycium barbarum L.) cv. Nixia 1 has been developed through axillary shoot proliferation. Driver and Kuniyuki Walnut (DKW) medium supplemented with 6-benzylaminopurine (BAP; 0.5 mg/L) and sucrose 3% w/v gave the best results compared with other basal media tested, with significantly improved production of multiple shoots direct from nodal segment explants, resulting in an average of 6.73 shoots/explant with an average of 7.45 nodes/shoot that would potentially form new explants. Rooting of shoot explants was carried out both in vitro and ex vitro with 0.5 and 1 mg/L of indole-3-butyric acid (IBA), with or without adding putrescine (160 mg/L). In all cases, rooting efficiency resulted very high, and putrescine was effective only when combined with a low concentration of auxin. Plantlets were hardened off in jiffy pots under greenhouse conditions, with a survival rate of more than 90%. Ex vitro rooting, performed by dipping in an aqueous solution of IBA 100 mg/L, is the preferred technique not only because rooting and acclimatization are very high but also reducing micropropagation to one phase is more economical.

scholarly journals The elaboration of a practical protocol for the micropropagation of several apple rootstock varieties

2016 ◽  
Vol 73 (2) ◽  
pp. 226
Author(s):  
Doina Clapa ◽  
Alexandru Fira ◽  
Manuela Simu ◽  
Monica Harta ◽  
Cristian Sisea
Keyword(s):  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Wheat Starch ◽  
Axillary Shoot ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Apple Rootstock ◽  
Axillary Shoot Proliferation ◽  
D 20

The apple rootstock varieties ‘MM 106/4’, ‘MM 106/6’, ‘D 18’, ‘D 20’, ‘JTE-H’ and ‘MR 09/4’ were multiplied in vitro on modified Murashige and Skoog media gelled with wheat starch (MSs) and supplemented with 0.7 mg/l BA, which provided intense axillary shoot proliferation. Among the genotypes we studied, MR 09/4 had the highest multiplication rate  (19.56), followed by D18 (15.36). The lowest multiplication rates were recorded in MM 106/6 (5.36) and in MM 106/4 (3.32). The use of the technique of direct ex vitro rooting and acclimatization in floating perlite provided rooting percentages of more than 90 %.

scholarly journals Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

2018 ◽  
Vol 10 (7) ◽  
pp. 275
Author(s):  
M. P. Machado ◽  
Erik Nunes Gomes ◽  
Felipe Francisco ◽  
André Felipe Bernert ◽  
João Carlos Bespalhok Filho ◽  
...  
Keyword(s):  
Culture Media ◽  
Humulus Lupulus ◽  
Ex Vitro Rooting ◽  
Field Conditions ◽  
Southern Brazil ◽  
Ex Vitro ◽  
Initial Development ◽  
Humulus Lupulus L ◽  
In Vitro Shoots

Tissue culture technique can be an important approach for the rapid propagation of Humulus lupulus L. (hop plant). The present study evaluated different culture media for hop plants (cv. Columbus) in vitro shoots multiplication, as well as ex vitro rooting and plantlets establishment under field conditions at Southern Brazil. The culture medium containing the plant growth regulator thidiazuron (TDZ) resulted in greater in vitro shoots multiplications (five shoots per explant). For microcuttings ex vitro rooting, indole-3-butyric acid (IBA) application at a concentration of 3000 mg L-1 resulted in higher rooting and survival percentages during acclimatization. After 90 days of acclimatization, the plantlets were transplanted under field conditions and had their development and productivity evaluated 122 days after transplanting. Micropropagated plants achieved 100% survival under field conditions. Nitrogen supply is highly necessary for hop plants initial development at Southern Brazil. Micropropagation is a viable technique for quality plantlet production of H. lupulus cv. Columbus.

scholarly journals In vitro propagation of Rubus macrocarpus Benth. and Rubus bogotensis Kunth, as an ex situ conservation strategy

2016 ◽  
Vol 66 (1) ◽  
Author(s):  
Belkys Adriana Pérez-Martínez ◽  
Sandra Liliana Castañeda-Garzón
Keyword(s):  
Indole Acetic Acid ◽  
Culture Media ◽  
Survival Rates ◽  
Conservation Strategy ◽  
Ex Situ ◽  
Stem Length ◽  
Ex Vitro ◽  
Rice Husks ◽  
Shoot Number

This research aimed to evaluate techniques for in vitro multiplication and rooting and ex vitro adaptation of wild blackberry plants (Rubus bogotensis Benth. and Rubus macrocarpus Kunth). In in vitro phases, the influence of culture media was evaluated based on Murashige & Skoog (MS) composition, with or without a 50% reduction in salts, vitamins, and myo-inositol and supplemented or not with benzylaminopurine (BAP), indole acetic acid (IAA), L-cysteine, agar, peat, perlite, and vermiculite. The ex vitro phase was based on a soil, rice husks, peat, and perlite substrate management. After two months of evaluation of the in vitro phases, it was determined that the MS medium at 50% salts, vitamins, and myo-inositol and supplemented with sucrose (15000 mg.l-1) and agar (5000 mg.l-1) was the most suitable for obtaining the highest values of apical length (cm), shoot number, rooting percentage, and root number. In ex vitro adaptation, the acclimation subphase, evaluated during three months, allowed to determine survival rates of 83.33% and 75% for R. macrocarpus and R. bogotensis, respectively. The average increase in stem length (cm) was 2.35 cm and 1.30 cm, respectively. In the greenhouse subphase, evaluated for four months, 100% plant survivorship was obtained, and 2.75 cm and 5.31 cm increases in average stem length (cm) for R. macrocarpus and R. bogotensis, were determined.

Long-term in vitro Storage Technique of Valuable Birch Genotypes and Plant Production on its Basis

2019 ◽  
pp. 57-67
Author(s):  
T.M. Tabatskaya ◽  
N.I. Vnukova
Keyword(s):  
Plant Production ◽  
High Survival ◽  
Ex Vitro ◽  
Regenerative Capacity ◽  
In Vitro Storage ◽  
Interspecific Differences ◽  
Regenerated Plants ◽  
Growing Conditions

A technique for the long-term (up to 27 years) in vitro storage of valuable birch genotypes under normal (25 °C, 2.0 klx, 16-h day and 8-h night) and low temperature (4 °C, 0.5 klx, 6-h day and 18-h night) growing conditions on hormone-free media has been described. The study explored for the first time the influence of different strategies to store the clones of Betula pubescens and B. pendula var. сarelica (6 genotypes) on the regenerative capacity of collection samples, adaptive potential of regenerated plants and plant production by the in vitro and ex vitro techniques. It was established that both storage strategies provided a persistently high survival rate (82-100%) and regenerative capacity of in vitro shoots (the multiplication coefficient of 4.2-6.3 and rhizogenic activity of 90-100%). The clones retained their characteristics of height growth under the in vitro and ex vitro conditions, and demonstrated intraclonal homogeneity and lack of signs of somaclonal variability. The plants showed substantial interspecific differences at the stage of multiplication and transfer to the greenhouse. The highest percentage of acclimated plants (75-98% depending on the clone genotype) was obtained after planting of micro plants straight in the greenhouse, which simplified the technology and made plant production less costly. long-term in vitro storage, birch, species, genotype, micropropagation, ex vitro adaptation, plant material

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Study of anatomical feature of in vitro and ex vitro regeneration plant of Sophora Alopecuroides l.

2019 ◽  
pp. 32-40
Author(s):  
Tsolmon M ◽  
Bayarmaa B ◽  
Oyunbileg Yu
Keyword(s):  
Success Rate ◽  
Ex Vitro Rooting ◽  
Vascular Cylinder ◽  
Ex Vitro ◽  
Anatomical Feature ◽  
Plant Survival ◽  
Anatomical Changes ◽  
Sophora Alopecuroides

The success of shoot and rooting from the nodal shoot segments of Sophora alopecuroides L. were induced in vitro condition. However, transferring and acclimatizing the plantlets to under soil or ex vitro condition were difficult. This study investigated plant survival with anatomical changes in plantlets while transferring from in vitro to ex vitro conditions to investigate vascular cylinder variations. The ex vitro rooting of the in vitro regenerated shoots, after having been treated with 500 mg/L IBA, showed a success rate of 80 per cent. These plantlets were rooted and acclimatized simultaneously in ex vitro condition.

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