The present study investigated whether racial microaggressions, specifically assumptions of inferiority, assumptions of criminality/second class citizenship, and microinvalidations had a relationship with depressive symptoms, and whether this relationship varied by age group (adults vs. adolescents) and race (Black and Latinx). This cross-sectional study compared 194 undergraduate college students who were all over the age of 18 to 168 high school juniors and seniors. All participants identified as either African American/Black or Latinx/Hispanic. The results indicated that respondents identifying as Black/African American, regardless of age, experience higher levels of assumptions of criminality/second class citizenship compared to Latinx respondents, F(2, 350) = 0.82, p = .442, ηp2 = .004. Results also indicated that, among Black/African American college students, but not high school students nor Latinx participants, higher levels of assumptions of inferiority were associated with depressive symptoms (b = .34, SE = 0.07, p < .001). Assumptions of criminality/second class citizenship, on the other hand, were not related with depressive symptoms (b = .06, SE = 0.08, p = .433). Lastly, regardless of race, high school students experienced more microinvalidations than college students, F(2, 350) = 3.97, p = .047, ηp2 =.013. These results underscore developmental changes in how students of color experience race and racism as they transition from adolescence into adulthood.