scholarly journals Effect of dietary sodium acetate on skin mucus immune parameters and expression of gene related to growth, immunity and antioxidant system in common carp (Cyprinus carpio) intestine

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Shabnam Nejadmoghaddam ◽  
Hien Van Doan
Keyword(s):  
Gene Expression ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Sodium Acetate ◽  
Control Group ◽  
Fish Diet ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Immune Parameters ◽  
Genes Expression

AbstractThe present study investigated the possible effects of including salt of short chain fatty acid, sodium acetate (SA), on skin mucus immune parameters and immune, antioxidant and growth-related genes expression in common carp. There is a little data available about the effective role of SA on immune, antioxidant and growth related genes expression as well as skin mucus immune parameters. The aim of this study was to analysis the effect of SA intake on these factors using common carp (Cyprinus carpio) as model organism. Two hundred and forty healthy common carp (mean weight = 15 ± 0.9 g) supplied and randomly stocked into 12 fiberglass tanks 200 L (20 fish per tank) assigned to four treatments and triplicates. The study was performed in a completely randomized design. The treatments were feeding carps with experimental diets containing different levels (0.0 [control], 0.5, 1 and 2%) of SA. The skin mucus total immunoglobulin and total protein levels in fish fed 2% SA showed significant increase compared to the control group (P < 0.05). Results showed a significant increase in the GH gene expression in 1 and 2% SA treatments (P < 0.05). The carps were fed with diet content 2% SA showed significantly increase in IGF-1 expression (P < 0.05). The expression of GSTa, and GPX (antioxidant genes) revealed a significant increase in the GSTa (fish fed SA at 1% and 2% levels) and GPX gene expression with fish fed 2% SA (P < 0.05). Supplementation of fish diet with SA induce slight elevation in the intestine of all immune-related genes (TNF-α, IL-1β, IL8 and Lyz) compared to the control group (P > 0.05). However, Lyz gene significantly up-regulated in 1 or 2% SA treatments. These results confirmed beneficial effects of SA as a feed additive in common carp culture.

scholarly journals Effect of dietary sodium acetate on skin mucus immune parameters and expression of gene related to growth, immunity and antioxidant system in common carp (Cyprinus carpio) intestine

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Shabnam Nejadmoghaddam
Keyword(s):  
Gene Expression ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Sodium Acetate ◽  
Control Group ◽  
Fish Diet ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Immune Parameters ◽  
Genes Expression

AbstractThe present study investigated the possible effects of including salt of short chain fatty acid, sodium acetate (SA), on skin mucus immune parameters and immune, antioxidant and growth-related genes expression in common carp. There is a little data available about the effective role of SA on immune, antioxidant and growth related genes expression as well as skin mucus immune parameters. The aim of this study was to analysis the effect of SA intake on these factors using common carp (Cyprinus carpio) as model organism. Two hundred and forty healthy common carp (mean weight = 15 ± 0.9 g) supplied and randomly stocked into 12 fiberglass tanks 200 L (20 fish per tank) assigned to four treatments and triplicates. The study was performed in a completely randomized design. The treatments were feeding carps with experimental diets containing different levels (0.0 [control], 0.5, 1 and 2%) of SA. The skin mucus total immunoglobulin and total protein levels in fish fed 2% SA showed significant increase compared to the control group (P < 0.05). Results showed a significant increase in the GH gene expression in 1 and 2% SA treatments (P < 0.05). The carps were fed with diet content 2% SA showed significantly increase in IGF-1 expression (P < 0.05). The expression of GSTa, and GPX (antioxidant genes) revealed a significant increase in the GSTa (fish fed SA at 1% and 2% levels) and GPX gene expression with fish fed 2% SA (P < 0.05). Supplementation of fish diet with SA induce slight elevation in the intestine of all immune-related genes (TNF-α, IL-1β, IL8 and Lyz) compared to the control group (P > 0.05). However, Lyz gene significantly up-regulated in 1 or 2% SA treatments. These results confirmed beneficial effects of SA as a feed additive in common carp culture.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

scholarly journals Skin Mucosal, Serum Immunity and Antioxidant Capacity of Common Carp (Cyprinus carpio) Fed Artemisia (Artemisia annua)

2020 ◽  
Vol 20 (3) ◽  
pp. 1011-1027 ◽  
Author(s):  
Iman Sarhadi ◽  
Ebrahim Alizadeh ◽  
Ehsan Ahmadifar ◽  
Hossein Adineh ◽  
Mahmoud A.O. Dawood
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Artemisia Annua ◽  
The Other ◽  
Control Group ◽  
Feed Conversion ◽  
Skin Mucus ◽  
Immune Parameters

AbstractIn this study, the effect of artemisia (Artemisia annua) leaves extract (ALE) on the immune system, growth performance and antioxidant capacity of common carp (Cyprinus carpio) was investigated. Fish with initial weight (90.32 ± 1.12 g) were fed various levels of ALE (0, 0.5, 1 and 1.5 g/kg) for 8 weeks. The results showed that the growth performance (FW, WG, SGR, PER, and LER) in fish fed 1 g/kg ALE was increased significantly (P<0.05) and the feed conversion ratio (FCR) was improved in fish fed 1 and 1.5 g/kg ALE in comparison to the control group (P<0.05). Skin mucus total protein was higher in fish fed diet containing 0.5 g/kg ALE than the other experimental groups (P<0.05), while the lysozyme, immunoglobulin and protease was increased by increasing of ALE in diets. Respiratory burst activity and serum immune parameters were higher in fish fed 0.5 g/kg ALE than the other treatments (P<0.05). Increasing of catalase (CAT) and decreasing of malondialdehyde (MDA) were observed in fish fed ALE, while glutathione peroxidase (GPx) increased only in fish fed 0.5 g/kg ALE. Hence, it can be concluded that ALE has considerable potential as a natural immunostimulant and growth promotor supplement for the common carp’s aquaculture.

scholarly journals Assessment of Responses of Common Carp, Cyprinus carpio, Exposed to Sub-Lethal Concentrations of Lead, by Molecular and Mucosal Biomarkers

10.4194/ga448 ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Fakhriyeh Omidi ◽  
Hojatollah Jafaryan ◽  
Rahman Patimar ◽  
Mohammad Harsij ◽  
Hamed Paknejad
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Protein Pattern ◽  
Innate Immune ◽  
Lead Contamination ◽  
Control Group ◽  
Hsp70 Gene ◽  
Skin Mucus ◽  
Expression Levels ◽  
The Impact

Heavy metals are one of the most persistent pollutants in waters, and molecular biomarkers as bioindicators could uncover early warning signals of stress suffered by organisms exposed to these pollutants. This research was conducted to explore the impact of lead contaminant on expression levels of hsp70 gene as well as protein pattern and innate immune indicators of skin mucus in Common carp as a biological model. The fish were exposed to different concentrations of Pb(NO3)2 as a source of lead (0, 7.875, 15.75, and 31.5 mg/L of lead) for 14 days. After the completion of the experiment, the liver and gill tissue plus mucus samples were collected from the fish. Next, the effects of exposure to sublethal doses of lead were investigated on expression levels of hsp70 gene, protein pattern, and innate immune indicators of skin mucus. The results indicated that the expression level of hsp70 gene significantly increased (at 7.5% & 30% LC50) in the liver tissue compared to the control group (P<0.05). The protein pattern of treatment samples was different from that of the control group. Also, in the mucus innate immune parameters, the levels of alkaline phosphatase increased significantly at high concentrations of lead contamination in comparison with the control group (P<0.05). The protein levels first revealed an ascending trend (P<0.05), and then a descending trend (at 30% LC50), though it was not a significant downward trend compared with the control group (P>0.05). The results suggested that these physiological indicators in Cyprinus carpio could be used as molecular and mucosal biomarkers for ecotoxicological studies.

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