scholarly journals Determination of some phenolic acids in Algerian propolis

2021 ◽  
Vol 32 (2) ◽  
pp. 120-124
Author(s):  
Abdelkrim Rebiai ◽  
Bachir Ben Seghir ◽  
Hadia Hemmami ◽  
Soumeia Zeghoud ◽  
Mohamed Lakhder Belfar ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Quantitative Method ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Plant Exudates ◽  
North And South

Abstract Propolis is a resinous material collected by bees from various plant exudates, rich in well-known phenolic compounds, such as phenolic acids, that are important to health. Extracts of propolis are very complex matrices that are hard to test. The purpose of this study was to characterize some of the propolis phenolics that were collected from five different districts in Algeria. The High-Performance Liquid Chromatography (HPLC), a modern quantitative method, has been adopted to identify the phenolic acids. Moreover, total phenolic content of four different phenolic acids were identified, with the most abundant being chlorogenic acid, followed by caffeic acid, gallic acid, and p-coumaric acid, the obtained ratios from phenolic acids being in the range of 52.193 to 148.151 μg/g, 0.043 to 7.128 mg/g, 0.328 to 0.440 mg/g and 0.328 to 0.440 mg/g, respectively. Overall, our analysis indicates that all the samples of propolis tested are healthy sources of phenolic acids and the significant differences in the concentrations of the acids were observed for propolis samples from north and south of Algeria. It is probably the effect of different conditions of the collection of the resin and secrets by bees.

scholarly journals Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Agnieszka Szopa ◽  
Michał Dziurka ◽  
Sebastian Granica ◽  
Marta Klimek-Szczykutowicz ◽  
Paweł Kubica ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
High Performance ◽  
Total Phenolic ◽  
Array Detector ◽  
Plant Materials

Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodiode array detector coupled to electrospray ionization ion trap mass spectrometry (UHPLC-DAD-ESI-MS3) and a high-performance liquid chromatography-diode array detector (HPLC-DAD). Using UHPLC-DAD-ESI-MS3, twenty-seven phenolic compounds from among phenolic acids and flavonoids were identified. Concentrations of three phenolic acids (neochlorogenic, chlorogenic and cryptochlorogenic acids) and eight flavonoids (hyperoside, rutoside, isoquercitrin, guaijaverin, trifolin, quercetin, kaempferol, and isorhamnetin) were determined using HPLC-DAD using reference standards. The highest total phenolic content was confirmed for the stem and leaf extracts collected in spring. The contents of phenolic compounds of in vitro biomasses were comparable to that in the fruit extracts. The methanolic extracts from the studied plant materials were evaluated for their antioxidant properties using various in vitro assays, namely free radicals scavenging estimation using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power (FRAP) and cupric-reducing antioxidant capacity (CUPRAC) as well as QUick, Easy, New, CHEap, and Reproducible CUPRAC (QUENCHER-CUPRAC) assays. A close relationship between the content of polyphenolic compounds in S. rubriflora and their antioxidant potential has been documented.

scholarly journals Diversity of Eleutherococcus genus in respect of biologically active compounds accumulation

2014 ◽  
Vol 60 (3) ◽  
pp. 34-43 ◽  
Author(s):  
Katarzyna Bączek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Raw Materials ◽  
Stem Bark ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Eleutheroside B

Summary Eight species of Eleutherococcus genus grown at Rogów Arboretum collection were compared in respect of biologically active compounds accumulation, i.e. eleutheroside B and E, phenolic acids and sterols. For the determination of content of these compounds in underground organs and stem bark high performance liquid chromatography was applied. The highest content of the sum of eleutherosides B and E was observed in underground organs and stem bark of E. leucorrhizus (322.0 and 300.8 mg × 100 g-1, respectively) and E. nodiflorus (218.9 and 363.5 mg × 100g-1, respectively). In the raw materials from E. senticosus, the content of these compounds was significantly lower (177.4 and 159.3 mg × 100 g-1, respectively). E divaricatus and E. setchuenensis were characterized by the lowest accumulation of these compounds in underground organs whereas E. divaricatus, E. sessiliflorus and E. giraldii - in stem bark. Four phenolic acids were identified in the investigated species, namely: chlorogenic, rosmarinic, ferulic and caffeic acids. The main phenolic acid in the analyzed species was chlorogenic acid. The content of this compound in underground organs varied from 102.1 (E. henryi) to 958.7 mg × 100 g-1 (E. leucorrhizus) and in stem bark from 26.7 (E. giraldii) to 542.5 mg × 100 g-1 (E. setchuenensis). The content of identified sterol compounds (sitosterol 3-O-β-D-glucoside /eleutheroside A / campesterol, stigmasterol) was relatively low, but higher in underground organs in comparison with stem bark.

scholarly journals Identification of Phenolic Acids and Flavonoids in Monofloral Honey from Bangladesh by High Performance Liquid Chromatography: Determination of Antioxidant Capacity

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammed Moniruzzaman ◽  
Chua Yung An ◽  
Pasupuleti Visweswara Rao ◽  
Mohammad Nurul Islam Hawlader ◽  
Siti Amirah Binti Mohd Azlan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Sugar Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
The Mean

The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. A total of five different phenolic acids were identified, with the most abundant being caffeic acid, benzoic acid, gallic acid, followed by chlorogenic acid and trans-cinnamic acid. The flavonoids, kaempferol, and catechin were most abundant, followed by myricetin and naringenin. The mean moisture content, total sugar content, and color characteristics of the honey samples were 18.36 ± 0.95%, 67.40 ± 5.63 g/100 g, and 129.27 ± 34.66 mm Pfund, respectively. The mean total phenolic acids, total flavonoid content, and proline content were 199.20 ± 135.23, 46.73 ± 34.16, and 556.40 ± 376.86 mg/kg, respectively, while the mean FRAP values and DPPH radical scavenging activity were 327.30 ± 231.87 μM Fe (II)/100 g and 36.95 ± 20.53%, respectively. Among the different types of honey, kalijira exhibited the highest phenolics and antioxidant properties. Overall, our study confirms that all the investigated honey samples are good sources of phenolic acids and flavonoids with good antioxidant properties.

Total phenolics and individual phenolic acids vary with light environment in Lindera benzoin

Botany ◽  
2010 ◽  
Vol 88 (11) ◽  
pp. 1007-1010 ◽  
Author(s):  
Christine M. Ingersoll ◽  
Richard A. Niesenbaum ◽  
Caitlin E. Weigle ◽  
Julia H. Lehman
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Dihydroxybenzoic Acid ◽  
Lindera Benzoin ◽  
Array Detection ◽  
Sun And Shade ◽  
Ferulic Acids ◽  
Shade Plants

The understory shrub Lindera benzoin L. experiences lower rates of herbivory in sun environments than in shade environments. The production of secondary metabolites (e.g., phenolic compounds with known plant defense properties) is one likely contributor to these observed differences in herbivory. This work determined the total phenolic content as well as the concentrations of several individual phenolic acids in L. benzoin leaves found in sun and shade habitats. Total phenolic concentrations were determined to be higher in leaves from sun plants than in those from shade plants (47.5 ± 2.4 vs. 28.6 ± 1.3 gallic acid equivalents, respectively). High-performance liquid chromatography with diode array detection was used to separate and quantify several individual phenolic acids, and specific compounds were identified based on their retention times and ultraviolet spectra. The concentrations of vanillic, chlorogenic, p-coumaric, and ferulic acids were shown to be statistically higher in leaves from sun plants than in those from shade plants (P < 0.05), whereas 2,5-dihydroxybenzoic acid and caffeic acid were not significantly different in L. benzoin leaves from sun versus shade habitats.

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