scholarly journals Antimicrobial susceptibility pattern and SCCmec types of methicillin-resistant coagulase-negative staphylococci from subclinical bovine mastitis in Hatay, Turkey

2014 ◽  
Vol 58 (4) ◽  
pp. 563-566 ◽  
Author(s):  
Özkan Aslantaş ◽  
Mehmet Ali Yılmaz ◽  
Ebru Şebnem Yılmaz ◽  
Cemil Kurekci
Keyword(s):  
Antimicrobial Susceptibility ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
High Rate ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Antimicrobial Susceptibility Pattern ◽  
Sccmec Types ◽  
Susceptibility Patterns ◽  
Staphylococcal Cassette Chromosome

Abstract Eighty-nine isolates of coagulase-negative staphylococci (CoNS) of eight species from subclinical bovine mastitis were screened for the phenotypic and genotypic methicilline-resistance. In addition, all methicillin-resistant (MR) isolates indicating the mecA gene were examined by PCR for the antimicrobial susceptibility patterns, and staphylococcal cassette chromosome mec (SCCmec) types were also determined by multiplex PCR. A total of 21 (23.6%) CoNS isolates were found to be resistant to oxacillin in broth microdilution assay. All isolates phenotypically resistant to oxacillin did not have the mecA gene, which was only found in 14.6% (13) of the isolates. Most MR-CoNS isolates were highly resistant to erythromycin (92.3%), fusidic acid (84.6%), penicillin (76.9%), and rifampycin (61.5%), and susceptible to mupirocin (100%), tetracycline (100%), vancomycin (100%), clindamycin (92.3%), and sulfamethoxazole-trimethoprim (69.2%). In conclusion, a high rate of antimicrobial resistance among MR-CoNS isolated from food producing animals emphasises the need for periodic surveillance of their resistance.

Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

2013 ◽  
Vol 16 (4) ◽  
pp. 687-692 ◽  
Author(s):  
M. Bochniarz ◽  
W. Wawron ◽  
M. Szczubiał
Keyword(s):  
Binding Protein ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Penicillin Binding Protein ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Phenotypic Resistance ◽  
Staphylococcus Warneri ◽  
Staphylococcus Haemolyticus ◽  
Lactam Ring

AbstractThe aim of this study was to determine the mechanisms of staphylococcal resistance to methicillin. CNS (n=100 isolates) were prepared from the mammary inflammatory secretions of 86 cows from farms located in the Lublin region.Methicillin-resistant isolates constituted 20.0% of all CNS. Staphylococcus sciuri (n=8) and Staphylococcus xylosus (n=6) were most abundant, followed by Staphylococcus chromogenes (n=3), Staphylococcus haemolyticus (n=2) and Staphylococcus warneri (n=1). The mecA gene was found in 50.0% of MRCNS (10.0% of all CNS isolates) belonging to two species: S. sciuri and S. xylosus. All mecA-positive isolates contained the protein of low affinity to penicillin (penicillin-binding protein 2a - PBP2a). The enzyme hydrolysing the β-lactam ring in antibiotics was detected in 40.0% of MRCNS; 10.0% of MRCNS isolates were characterised by the presence of the mecA gene and ability to produce β-lactamase. The remaining 20.0% of MRCNS isolates showing phenotypic resistance to methicillin were mecA gene-negative and were not able to produce β-lactamase.

scholarly journals Molecular Typing and Antimicrobial Susceptibility of Methicillin-ResistantStaphylococcus aureusIsolated from Bovine Milk in Tanzania

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Jibril Mohammed ◽  
Michael Henry Ziwa ◽  
Yaovi Mahuton Gildas Hounmanou ◽  
Adela Kisanga ◽  
Huruma Nelwike Tuntufye
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bovine Milk ◽  
Raw Milk ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Local Markets ◽  
Antimicrobial Susceptibility Pattern

Methicillin-resistantStaphylococcus aureus(MRSA) in raw milk can be transmitted from animals to humans, and in Tanzania raw milk is sold in local markets and consumed as purchased. This study was performed to determine the molecular characteristics and antimicrobial susceptibility pattern of MRSA strains isolated from raw bovine milk sold at local markets in Tanzania. A total of 117 raw milk samples were cultured on Baird-Parker medium to isolateS. aureusand PCR was used for amplification ofgltBgene forS. aureusidentification and the presence ofmecAgene for methicillin-resistant strains. Coagulase-negative (CN)S. aureuswere reconfirmed using tube coagulase, DNase, and API Staph tests. MRSA isolates werespatyped whereas antimicrobial susceptibility testing was performed by the disc diffusion method. Forty-six coagulase positives (CP) and two CNS. aureuswere identified. Most strains were resistant to penicillin (72%), and 3 isolates: 2 CNS. aureusand 1 coagulase-negative Staphylococci (CNS), were phenotypically resistant to vancomycin, oxacillin, and cefoxitin and were confirmed to carrymecA.Resistance to clindamycin, trimethoprim-sulfamethoxazole, and tetracycline was 23.9%, 30.4%, and 41.3%, respectively. Twelve isolates exhibited multidrug resistance; however, only onemecApositive strain among the three was typeable and belonged tospatype t2603. This study reports for the first time the presence of CN variant of MRSA, which was assigned the spa type t2603, and the presence of multidrug resistantS. aureusisolates from bovine milk in Morogoro, Tanzania.

scholarly journals Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

2020 ◽  
Vol 40 (5) ◽  
Author(s):  
Effat Abbasi Montazeri ◽  
Sakineh Seyed-Mohammadi ◽  
Aram Asarehzadegan Dezfuli ◽  
Azar Dokht Khosravi ◽  
Maryam Dastoorpoor ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Sccmec Type ◽  
Meca Gene ◽  
Clinical Isolates ◽  
Resistance Pattern ◽  
Type I ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Drug Resistance Pattern ◽  
Sccmec Types

Abstract Today methicillin resistant coagulase-negative staphylococci (MR-CoNS) are important in terms of causing significant nosocomial infections. Besides, MR-CoNS are confirmed as the reservoir of SCCmec elements that carry mecA (methicillin-resistant) gene. Hence, the present study was designed to evaluate the susceptibility pattern, prevalence and diversity of SCCmec types I, II, III, and IV in MR-CoNS strains. In this cross-sectional study, 44 clinical isolates of MR-CoNS were identified using the cefoxitin disc method and further confirmation by polymerase chain reaction (PCR) amplification of the mecA gene. Antimicrobial susceptibility of isolates was investigated by disc diffusion. The identification of CoNS was done by amplification and sequencing of the tuf gene. Multiplex PCR method was done for the determination of SCCmec types. In the present study, the Staphylococcus epidermidis and Staphylococcus haemolyticus were the most predominant isolates with a prevalence of 45.4%. The highest resistance rates were observed against erythromycin (84.1%) and clindamycin (75%). Multiplex PCR revealed the SCCmec type I as the predominant type in the present study. Our study showed that there was no significant relationship between the presence of different types of SCCmec elements and resistance to antibiotics. The present study highlighted a frequent prevalence of MR-CoNS harboring SCCmec type genes in Ahvaz, southwest of Iran. Thus, the molecular typing and periodical monitoring of their drug resistance pattern should be considered in national stewardship programs to designing useful antibiotic prescription strategies.

scholarly journals Transformation of commensal to a pathogen: Blood stream infection due to coagulase negative staphylococci among patients attending tertiary care hospital in North India

2021 ◽  
Vol 7 (2) ◽  
pp. 71-76
Author(s):  
Anupam Das ◽  
Vikramjeet Singh ◽  
Pranshu Pandey ◽  
Manodeep Sen ◽  
Jyotsna Agarwal
Keyword(s):  
Antimicrobial Susceptibility ◽  
Blood Stream Infection ◽  
Blood Stream ◽  
North India ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Antimicrobial Susceptibility Pattern ◽  
Culture Positive

Aim of this study was to know the prevalence of Coagulase Negative Staphylococci (CoNS) in Blood Stream Infection (BSI) among patients attending a Superspeciality hospital in North India. Objective of this study was to compare incidence of CoNS in ICU and ward patients suffering from BSI and their antimicrobial susceptibility pattern. This retrospective hospital based study was conducted in the Microbiology Department, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow for a period of two years (January, 2017 -December, 2018). Blood samples from a total of 6498 patients from Out-Patient, In-Patient and Intensive care unit departments were subjected to aerobic and anaerobic bacterial culture. Culture positive broth was subcultured on Blood Agar and MacConkey Agar to isolate pathogens in pure culture. Pure cultured isolates were tested for antimicrobial susceptibility pattern by Kirby Bauer Disk Diffusion method as per CLSI 2018.During the study period, out of total 3284 samples in 2017 and 3214 in 2018, 663 and 595 were found to be culture positive respectively.Staphylococci were isolated from 636 (9.78%) patients (10.2% in 2017 and 9.2% in 2018). Staphylococcus aureus was isolated from 18.8% and CoNS from 81.2% of total Staphylococcal isolates. Among CoNS isolates 70.7% were found to be Methicillin Resistant CoNS (MRCoNS). This study observed CoNS as a major cause of BSI as compared to Staphylococcus aureusThe significance of CONS bacteremia should be evaluated better in light of clinical profile of the patient. Better screening and infection control practices in the future can decrease the rate of methicillin resistant CoNS in our centre.

scholarly journals Occurrence and characteristics of methicillin resistant Staphylococcus aureus and methicillin resistant coagulase-negative staphylococci in raw milk manufacturing

2012 ◽  
Vol 29 (Special Issue) ◽  
pp. S11-S16 ◽  
Author(s):  
M. Vyletělová ◽  
H. Vlková ◽  
I. Manga
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Staphylococcal Cassette Chromosome

For monitoring the occurrence of MRSA (methicillin resistant Staphylococcus aureus) and MR-CNS (methicillin resistant coagulase-negative staphylococci), cow’s, goat’s, and sheep’s milks (bulk milks and individual samples) were investigated. Human nasal and throat swabs of the farm staff and nasal swabs of animals were also investigated as well. In total 1729 samples were examined and 634 strains were isolated by means of the cultivation method and used in this study. Generic identification of the staphylococci isolates was done performed by biochemical tests and all S. aureus and CNS isolates were checked by the PCR method for the presence of mecA gene which is responsible for methicillin resistance. The presence of the staphylococcal cassette chromosome mec (SCCmec), Panton-Valentine leukocidin (pvl) and genes encoding toxic shock syndrome toxin (tst) was detected in all strains confirmed as MRSA. The species were also examined for antimicrobial susceptibility by using disk diffusion method with antibiotic disks. S. aureus was the most frequently identified species from the samples tested (n = 557; 32.2%), followed by S. haemolyticus (n = 32; 1.9%), S. chromogenes (n = 24; 1.4%), S. epidermidis (n = 20; 1.2%), and S. caprae (n = 1; 0.16%). Among the resistant staphylococci (n = 49), S. aureus (n = 25; 51%) was found the most frequently, followed by S. epidermidis (n = 17; 34.7%), S. chromogenes (n = 6; 12.2%), and S. haemolyticus (n = 1; 2%). The resistant Staphyloccocus sp. occurred mainly in cow’s milk (MRSA, S. epidermidis, S. chromogenes, S. haemolyticus) and in animal’s swabs (S. epidermidis). One MRSA was also found in goat’s milk and one was isolated from human swab. No resistant strains were found in sheep’s milk. The negative results of the analysed genes presence (pvl, tst) were identical with all MRSA tested. The staphylococcal cassette chromosome mec (SCCmec) was classified as type IV or V.

scholarly journals The SCCmec Types and Antimicrobial Resistance among Methicillin-Resistant Staphylococcus Species Isolated from Dogs with Superficial Pyoderma

2021 ◽  
Vol 8 (5) ◽  
pp. 85
Author(s):  
Yuttana Chanayat ◽  
Areerath Akatvipat ◽  
Jeff B. Bender ◽  
Veerasak Punyapornwithaya ◽  
Tongkorn Meeyam ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Sccmec Type ◽  
Small Animal ◽  
Control Measures ◽  
Infection Prevention And Control ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Type V ◽  
Staphylococcal Cassette Chromosome

This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.

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