scholarly journals Chlorophyll Fluorescence and Drought Tolerance in a Mapping Population of Durum Wheat

2021 ◽  
Vol 70 (3-4) ◽  
pp. 123-134
Author(s):  
Chafia Larouk ◽  
Fatima Gabon ◽  
Zakaria Kehel ◽  
Abdelhamid Djekoun ◽  
Miloudi Nachit ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Drought Stress ◽  
Genetic Variability ◽  
Drought Tolerance ◽  
Durum Wheat ◽  
Chlorophyll Fluorescence Parameters ◽  
Grain Yields ◽  
Fluorescence Parameters ◽  
Drought Conditions ◽  
Wheat Lines

Summary This study was conducted to compare and estimate the genetic variability among durum wheat lines in response to drought according to their grain yields and physiological traits. The use of fluorescence parameters as drought selection criteria for durum wheat was investigated in a population of 249 recombinant inbred lines (RILs) derived from a cross between two cultivars of durum wheat: ‘Svevo’ and ‘Kofa’. The durum wheat RILs considered were analyzed to determine the relationship between their grain yields and chlorophyll fluorescence parameters at the grain filling stage under drought stress (rainfed) and supplemental irrigation (irrigated) conditions during the 2004 and 2005 seasons at the ICARDA center. The results obtained show that the durum wheat line performance was influenced by drought stress, indicating significant differences between the grain yields and fluorescence parameters recorded under drought rainfed (RF) and irrigation (IR) conditions. Significant differences between the experimental groups of durum wheat RILs (P<0.05) were only recorded under drought conditions, with the exception of leaf water potential (Lwp). Under such conditions, nearly all the parameters examined were significantly increased in the high-yielding group (with the exception of the Que parameter), thus revealing the genetic variability of the durum wheat lines considered in response to drought stress. The potential quantum efficiency of photosystem II (Fv/Fm) was found to be positively associated with the grain yield parameter. The mean values of Fv/Fm in both the high- and low-yielding groups significantly dropped under drought stress (0.71 and 0.68, respectively) compared to the Fv/Fm values recorded under irrigated conditions (0.80 and 0.81). Under drought conditions, slopes were highly significant (P<0.001) nearly for all the fluorescence parameters examined (with the exception of CHLSPAD) compared to those recorded under irrigation conditions. It was concluded that chlorophyll content (SPAD), F0, Fm, Fv, Fv/Fm, Lwp, and Que could be used as additional indicators in screening wheat germplasm for drought tolerance.

scholarly journals Chlorophyll fluorescence response of wheat to exogenous application of growth regulators under terminal drought stress

2015 ◽  
Vol 70 (1) ◽  
pp. 13
Author(s):  
Hamid Mohammadi ◽  
Mohsen Janmohammadi ◽  
Naser Sabaghnia
Keyword(s):  
Chlorophyll Fluorescence ◽  
Drought Stress ◽  
Electron Transport ◽  
Quantum Yield ◽  
Photochemical Quenching ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Exogenous Application ◽  
Terminal Drought ◽  
Terminal Drought Stress

<p>Drought stress negatively affects plant photosynthesis and disturbs the electron transport activity. Evaluation of the chlorophyll fluorescence parameters might reflect influence of the environmental stress on plants and can be applied as an indicator of the primary photochemistry of photosynthesis. In current study the effect of foliar application of benzylaminopurine (BAP, a synthetic cytokinin) and abscisic acid (ABA) on chlorophyll fluorescence parameters of relatively drought tolerant (Pishtaz) and susceptible (Karaj3) bread wheat genotypes under well watered and terminal water deficit condition have been evaluated. Terminal drought was induced by withholding water at anthesis stage (Zadoks scale 65). Results showed that coefficient of non-photochemical quenching of variable fluorescence (qN), quantum yield of PS II photochemistry (ΦPSII) and photochemical quenching (qP) were affected by hormone spray treatments. So that evaluation of parameters at 7 day after foliar treatments revealed that ABA significantly increased electron transport rate (ETR) and qN while considerably decreased ΦPSII, gs and maximum quantum yield of photosystem II (Fv/Fm). However exogenous application of cytokinin could increase gs, Fv/Fm and ΦPSII and the highest value of these parameters was recorded in <em>cytokinin </em>treated plants of Pishtaze cv. under well watered condition. Nevertheless, evaluation of the parameters in different periods after spraying showed that with approaching the maturity stage some traits like as gs, Fv/Fm and ETR significantly decreased in both genotypes. Evaluation of gs and Chlorophyll fluorescence parameters of genotypes between different irrigation levels showed that although cv. Pishtaz showed higher performance of PSII under well watered condition, it failed to maintain its superiority under stress condition. This finding suggests that some more responsive parameter like gs, Fv/Fm and ΦPSII can be considered as reliable indicator for understanding the biochemical and physiological effects of exogenous application of phytohormones under terminal drought stress.</p>

Genetic variability and identification of quantitative trait loci affecting plant growth and chlorophyll fluorescence parameters in the model legume Medicago truncatula under control and salt stress conditions

2014 ◽  
Vol 41 (9) ◽  
pp. 983 ◽  
Author(s):  
Sarah Exbrayat ◽  
Georges Bertoni ◽  
Mohamad Reza Naghavie ◽  
Ali Peyghambari ◽  
Mounavar Badri ◽  
...  
Keyword(s):  
Salt Stress ◽  
Chlorophyll Fluorescence ◽  
Quantitative Trait Loci ◽  
Genetic Variability ◽  
Plant Growth ◽  
Medicago Truncatula ◽  
Quantitative Trait ◽  
Chlorophyll Fluorescence Parameters ◽  
Model Legume ◽  
Fluorescence Parameters

Salinity is one of the major stresses that limits crop production worldwide and affects most physiological activities in plants. In order to study the genetic control of salt stress in the model legume Medicago truncatula Gaertn., an experiment was undertaken to determine the genetic variability and to identify quantitative trait loci (QTLs) controlling several traits related to plant growth and physiology in a population of recombinant inbred lines. Shoot and root DW, relative water content, leaf area, chlorophyll content, chlorophyll fluorescence parameters, and Na+ and K+ in shoots and roots were measured. The experiment was carried out with three replications. ANOVA showed a large genetic variation and transgressive segregation for the traits studied, suggesting putative complex tolerance mechanisms. A total of 21 QTLs were detected under control conditions and 19 QTLs were identified under 100 mm salt stress conditions, with three QTLs being common to both situations. The percentage of total phenotypic variance explained by the QTLs ranged from 4.6% to 23.01%. Overlapping QTLs for different traits were also observed, which enables us to discriminate independent traits from linked ones. The results should be helpful information for further functional analysis of salt tolerance in M. truncatula.

scholarly journals Drought stress strengthens the link between chlorophyll fluorescence parameters and photosynthetic traits

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10046 ◽  
Author(s):  
Jie Zhuang ◽  
Yonglin Wang ◽  
Yonggang Chi ◽  
Lei Zhou ◽  
Jijing Chen ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Drought Stress ◽  
Terrestrial Ecosystems ◽  
Stomatal Limitation ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Total Chlorophyll Content ◽  
Leaf Level ◽  
Photosynthetic Traits ◽  
Quantum Efficiency Of Psii

Chlorophyll fluorescence (ChlF) has been used to understand photosynthesis and its response to climate change, particularly with satellite-based data. However, it remains unclear how the ChlF ratio and photosynthesis are linked at the leaf level under drought stress. Here, we examined the link between ChlF ratio and photosynthesis at the leaf level by measuring photosynthetic traits, such as net CO2 assimilation rate (An), the maximum carboxylation rate of Rubisco (Vcmax), the maximum rate of electron transport (Jmax), stomatal conductance (gs) and total chlorophyll content (Chlt). The ChlF ratio of the leaf level such as maximum quantum efficiency of PSII (Fv/Fm) is based on fluorescence kinetics. ChlF intensity ratio (LD685/LD740) based on spectrum analysis was obtained. We found that a combination of the stomatal limitation, non-stomatal limitation, and Chlt regulated leaf photosynthesis under drought stress, while Jmax and Chlt governed the ChlF ratio. A significant link between the ChlF ratio and An was found under drought stress while no significant correlation in the control, which indicated that drought stress strengthens the link between the ChlF ratio and photosynthetic traits. These results suggest that the ChlF ratio can be a powerful tool to track photosynthetic traits of terrestrial ecosystems under drought stress.

The quantum yield of photosynthetic electron transport evaluated by chlorophyll fluorescence as an indicator of drought tolerance in durum wheat

1995 ◽  
Vol 125 (3) ◽  
pp. 325-329 ◽  
Author(s):  
Z. Flagella ◽  
D. Pastore ◽  
R. G. Campanile ◽  
N. Di Fonzo
Keyword(s):  
Chlorophyll Fluorescence ◽  
Electron Transport ◽  
Quantum Yield ◽  
Drought Tolerance ◽  
Durum Wheat ◽  
Photosynthetic Electron Transport ◽  
Fluorescence Measurement ◽  
Chlorophyll Fluorescence Parameters ◽  
Percentage Decrease ◽  
Induction Curve

SUMMARYThe chlorophyll fluorescence parameters ΔF/Fm′ and Fv′/Fm′, related respectively to the quantum yield of photosynthetic electron transport and to the efficiency of excitation capture by the open centres of photosystem II, have been evaluated as possible indicators of drought tolerance in durum wheat. ΔF/Fm′ and Fv′/Fm′ measurements were carried out on excised leaves, both control and dehydrated, of 25 cultivars. ΔF/Fm′ and Fv′/Fm′ values were obtained at two times after the start of fluorescence measurement: at 14 s, i.e. during the induction curve (ΔF/Fm′14s and Fv′/Fm′14s) and at 200s, i.e. at steady state fluorescence (ΔF/Fm′200s and Fv′/Fm′200s).In dehydrated leaves a mean significant decrease of 20% (P < 0·001) was observed in ΔF/Fm′14s values. In contrast, no great differences were observed between control and dehydrated leaves with regard to ΔF/Fm′200s, Fv′/Fm′14s and Fv′/Fm′200s.The percentage decrease of ΔF/Fm′14s after dehydration was correlated with the drought susceptibility index (DSI) of the cultivars, evaluated on a yield basis and a significant correlation (r = 0·72, P < 0·001) was found.

Sign in / Sign up

Export Citation Format

Share Document