scholarly journals Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa

2020 ◽  
Vol 47 (1) ◽  
pp. 34-44
Author(s):  
Jana Konôpková ◽  
Dominika Košútová ◽  
Peter Ferus
Keyword(s):  
Primary Culture ◽  
Fruit Ripening ◽  
Culture Initiation ◽  
High Positive Correlation ◽  
Initiation Phase ◽  
Primary Explants ◽  
Basal Media ◽  
Bud Opening ◽  
Opening Stage

AbstractThe influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine Magnolia genotypes, including M. stellata /Sieb. & Zucc./Maxim., M. × soulangeana ‘Rustica Rubra’, M. denudata Desr., M. × soulangeana ‘Alexandrina’, M. liliiflora Desr., M. officinalis var. biloba Rehd. & Wils., M. salicifolia Maxim., M. × soulangeana ‘Lennei’, and M. kobus DC, was evaluated. The highest efficiency of primary culture initiation of seven Magnolia genotypes (except for M. liliiflora and M. salicifolia) was achieved from primary explants collected in the bud opening stage. A high positive correlation was found between total tannins and efficiency of the primary culture initiation at the fruit ripening stage (r = 0.833). Standardi and Catalano medium (S2) with 0.5 mg l−1 of 6-benzylaminopurine (BAP) was the most appropriate for multiplication of M. × soulangeana ‘Alexandrina’, whereas tissue cultures of M. × soulangeana ‘Lennei’ proliferated and grew better on S2 medium with 1.0 mg l−1 of BAP and 1.0 g l−1 of polyvinylpyrrolidone. The requirements for the composition of basal media and concentration of PGRs in the initiation and multiplication stages of micropropagation of various Magnolia species and cultivars are genotype-specific.

scholarly journals In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

2019 ◽  
Vol 33 ◽  
Author(s):  
Cláudio Rodrigues Rezende Costa ◽  
Bruna Rabelo Amorim ◽  
Sandra Márcia Mazutti da Silva ◽  
Ana Carolina Acevedo ◽  
Pérola de Oliveira Magalhães ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Eugenia Dysenterica

Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver

1992 ◽  
Vol 10 (3) ◽  
pp. 205-215 ◽  
Author(s):  
Scott L. Nyberg ◽  
Russell A. Shatford ◽  
William D. Payne ◽  
Wei-Shou Hu ◽  
Frank B. Cerra
Keyword(s):  
Primary Culture ◽  
Rat Hepatocytes ◽  
Bioartificial Liver ◽  
Collagen Gels ◽  
In Vitro System

Isolation and primary culture of rat cerebral microvascular endothelial cells for studying drug transport in vitro

1996 ◽  
Vol 36 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Nobuhiro Ichikawa ◽  
Kohji Naora ◽  
Hidenari Hirano ◽  
Michio Hashimoto ◽  
Sumio Masumura ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Primary Culture ◽  
Drug Transport ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary

2010 ◽  
pp. 12-17 ◽  
Author(s):  
Gyula Oros ◽  
László Vajna ◽  
Klára Balázs ◽  
Zoltán Fekete ◽  
Zoltán Naár ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Fungal Pathogen ◽  
Colletotrichum Gloeosporioides ◽  
Rapid Development ◽  
Sour Cherry ◽  
Control Measures ◽  
Yield Losses ◽  
Trichoderma Species ◽  
Special Regard

Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last decades. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.

scholarly journals In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

2011 ◽  
Vol 63 (1) ◽  
pp. 209-215 ◽  
Author(s):  
S. Uranbey
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Ornamental Plant ◽  
Immature Embryos ◽  
Induction Medium ◽  
Ms Medium ◽  
Culture Initiation ◽  
Mineral Salts ◽  
Font Color

A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on ? strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a><u></b></font>

scholarly journals Seleksi In Vitro dan Pengujian Mutan Tanaman Pisang Ambon Kuning untuk Ketahanan terhadap Penyakit Layu Fusarium

2016 ◽  
Vol 9 (2) ◽  
pp. 66
Author(s):  
Deden Sukmadjaja ◽  
Ragapadmi Purnamaningsih ◽  
Tri P. Priyatno
Keyword(s):  
Fusarium Wilt ◽  
In Vitro Selection ◽  
Fusaric Acid ◽  
Mutation Breeding ◽  
Induce Mutagenesis ◽  
Fusarium Wilt Of Banana ◽  
Basal Media ◽  
Generative Phase ◽  
Race 4

<p>Fusarium wilt of banana (Musa spp.) caused by<br />Fusarium oxysporum f. sp. cubense (Foc) is the most serious<br />problem faced in banana cultivation in terms of plant<br />productivity and fruit quality. Mutation breeding is one of the<br />alternative method that can be applied in producing new<br />banana cultivar. Mutants can be induced by chemical<br />mutagen such as ethyl methane sulfonate (EMS) followed by<br />in vitro selection and then evaluation of the mutants to<br />fusarium wilt disease in glasshouse and Foc infected field.<br />The aim of this research was obtained EMS induced and in<br />vitro selected mutants of banana var. Ambon Kuning and<br />evaluated Foc disease resistant clones in glasshouse and<br />Foc infected field. The first step to obtain the explants for<br />this research was initiation and formation of multiple bud<br />clumps (MBC) using MS basal media supplemented with 5,<br />10, and 20 mg/l of benzyladenin. Plant regeneration of MBC<br />was also studied by using MS media containing 0, 0.2, and 1<br />mg/l of benzyladenin. To induce mutagenesis, MBC was<br />soaked in 0.1, 0.3, and 0.5% (v/v) EMS for 1, 2, and 3 hours.<br />The assesment of resistant MBC mutants to Fusarium<br />phytotoxin was conducted by using fusaric acid (FA) as<br />selection agent in concentration of 30, 45, and 60 ppm.<br />Putative mutant plants produced by in vitro selection were<br />further tested using spore solution of Foc race 4 in<br />glasshouse. Meanwhile, Foc resistance assesment in the<br />infected field was conducted in Pasirkuda Experimental<br />Station, Bogor Agricultural University. The results showed<br />that MBC can be formed in MS basal media supplemented<br />with 10 or 20 mg/l benzyladenin. The EMS played a role in<br />obtaining mutants by producing 68 MBC putative mutants<br />tolerant to Foc based on FA selection. Further evaluation in<br />the glasshouse was obtained 64 Foc resistant plants from<br />391 putative mutants produced by in vitro selection.<br />Evaluation in the Foc infected field showed six clones<br />survived until generative phase (12 month of age).</p>

scholarly journals In Vitro Effects of Yessotoxin on a Primary Culture of Rat Cardiomyocytes

2008 ◽  
Vol 106 (2) ◽  
pp. 392-399 ◽  
Author(s):  
Valeria Dell'Ovo ◽  
Elena Bandi ◽  
Tamara Coslovich ◽  
Chiara Florio ◽  
Marina Sciancalepore ◽  
...  
Keyword(s):  
Primary Culture ◽  
Rat Cardiomyocytes ◽  
In Vitro Effects

Appearance and evolution of calcium currents and contraction during the early post-fusional stages of rat skeletal muscle cells developing in primary culture

Development ◽  
1993 ◽  
Vol 117 (3) ◽  
pp. 1153-1161
Author(s):  
C. Cognard ◽  
B. Constantin ◽  
M. Rivet-Bastide ◽  
N. Imbert ◽  
C. Besse ◽  
...  
Keyword(s):  
Primary Culture ◽  
Contractile Activity ◽  
Primary Cultures ◽  
Calcium Currents ◽  
Calcium Stores ◽  
Developmental Evolution ◽  
Time To Peak ◽  
Mechanical And Electrical Properties ◽  
Developing Muscle

Primary cultures from enzymatically dissociated satellite cells of newborn rat skeletal muscles enabled developmental in vitro studies of mechanical and electrical properties during the first steps of myogenesis. The present work focused on the appearance, evolution and roles of two types of calcium currents (ICa,T and ICa,L) and of depolarization-induced contractile activity during the early stages of muscle cell development in primary culture. Prefusional mononucleated cells (myoblasts), young myotubes of 1 day (with less than 10 nuclei) or 2–3 days (more than 9 nuclei) and myoballs from 4–6, 7–9, 10–12 and 13–16 days cultures were patch-clamped (whole-cell configuration), and calcium currents and contraction simultaneously recorded. Sodium but not calcium currents could be recorded at the myoblast stage. In young myotubes (1 day), ICa,L was present with high incidence as compared to ICa,T, which was poorly expressed. Contractile responses appeared at the next stage (2-3 days) while the occurrence of ICa,T progressively increased. This developmental evolution of the calcium currents and contraction expression was accompanied by some changes in their characteristics: the ICa,T/ICa,L amplitudes ratio progressively increased and the time-to-peak of contraction progressively decreased with the age of myoballs. Physiological functions for calcium currents in developing muscle are suggested and discussed: ICa,T, which is transiently expressed, could be involved in the pacemaker-like activity while ICa,L could serve as an early contraction triggering mechanism and/or initially to fill and then to maintain the intracellular calcium stores.

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