scholarly journals Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Cassandra Ceccopieri ◽  
Joanna Skonieczna ◽  
Jan P. Madej
Keyword(s):  
Connective Tissue ◽  
Staining Method ◽  
Diagnostic Value ◽  
Collagen I ◽  
Staining Procedure ◽  
Absolute Alcohol ◽  
Collagen Fibres ◽  
Bright Yellow

Abstract Introduction The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres. Material and Methods The saffron solution was obtained by dissolving ground saffron stigmas in absolute alcohol. In order to test the HES method for its staining ability on four main types of collagen (I, II, III, and IV), specific tissues (skin, tooth, cartilage, aorta, spleen, and penis) were chosen. Results The procedure showed a sharp differentiation between muscle, stained red or pink, and connective tissue, stained bright yellow or orange. Conclusion HES allows the diagnosis of reticulin fibrosis undetected in HE and in previous saffron staining procedures. HES represents an advantageous alternative to HE staining giving highly reproducible results with high diagnostic value.

Diagnostic value of connective tissue metabolites in Schistosoma mansoni related liver disease

Acta Tropica ◽  
1999 ◽  
Vol 73 (2) ◽  
pp. 153-164 ◽  
Author(s):  
R. Kardorff ◽  
C. Mugashe ◽  
R.M. Gabone ◽  
C. Mahlert ◽  
E. Doehring
Keyword(s):  
Liver Disease ◽  
Connective Tissue ◽  
Schistosoma Mansoni ◽  
Diagnostic Value ◽  
Related Liver Disease ◽  
Tissue Metabolites

Diagnostic value of assessment of coronary calcification in dysplastic heart

2021 ◽  
Vol 19 (1) ◽  
pp. 75-77
Author(s):  
L. T. Pimenov ◽  
◽  
V. V. Remnyakov ◽  
M. Yu. Smetanin ◽  
E. N. Avdeev ◽  
...  
Keyword(s):  
Connective Tissue ◽  
System Development ◽  
Heart Diseases ◽  
Coronary Calcification ◽  
Diagnostic Value ◽  
Conduction Disorders ◽  
Increased Risk ◽  
Structural Heart Diseases ◽  
Connective Tissue Dysplasia ◽  
Functional Features

The problem of heart connective tissue dysplasia syndrome is extremely relevant due to the increased risk of rhythm and conduction disorders, infectious endocarditis, thromboembolism and sudden cardiac death (SCD). Structural heart diseases (SHD) are manifestations of minor anomalies of the cardiovascular system development. Dysplastic heart refers to the combination of constitutional, topographical, anatomical, and functional features of the heart in a patient with connective tissue dysplasia (CTD). The standard for the diagnosis of coronary calcification (CC), one of the known predictors of coronary heart disease (CHD) and complications of cardiovascular diseases (CVD), is multispiral computed tomography (MSCT).

scholarly journals Characterisation of Morphology in Musculus Semitendinosus Tendon Used for Anterior Cruciate Ligament Reconstruction: A Pilot Study

2020 ◽  
Vol 18 (1) ◽  
pp. 1-6
Author(s):  
Janis Davis Osipovs ◽  
Mara Pilmane ◽  
Modris Ciems
Keyword(s):  
Anterior Cruciate Ligament ◽  
Morphological Changes ◽  
Cruciate Ligament ◽  
Collagen I ◽  
Collagen Fibres ◽  
Nerve Fibres ◽  
Tissue Ischemia ◽  
Moderate Number ◽  
Collagen Iii ◽  
Anterior Cruciate

SummaryIntroductionAnterior cruciate ligament (ACL) rupture is very common in athletes. In the general population, incidence is approximately 33 per 100 000 people.Aim of the studyThe aim of the study was the evaluation of morphological changes in the musculus semitendinosus tendon used for the reconstruction of ACL to understand the quality of most common for surgery used material.Material and methodsThe materials were obtained from four ACL autologous hamstring reconstruction surgeries. The tissue was stained with hematoxyllin and eosin and with immunohistochemical (IMH) staining of PGP9.5, VEGF, collagen I and collagen III. The material was evaluated with semiquantitative method.ResultsRoutine staining showed practically unchanged tendon structure, with one exception when sclerotic blood vessels were observed in endotenon. Collagen III IMH demonstrated moderate to numerous positive collagen fibres in two cases, but in other two there were only few positive structures seen. Collagen I IMH showed few to moderate number of positive collagen fibres in all cases. In two cases, moderate number of PGP9.5 positive nerve fibres was observed and in two other cases occasional to few number of positive structures was detected. PGP 9.5 marked higher number of nerve fibres in peritenon than in endotenon. Numerous VEGF positive endotheliocytes were observed in two cases, but in two other cases VEGF positive endotheliocytes were occasional.ConclusionsTendon of musculus semitendinosus displays two patterns of distribution of tissue ischemia, neuropeptide containing innervation and collagen I and III. Collagen III is thought to be evaluated as a response of tendon to the ischemia and intensive innervation, while increase of collagen I probably is related to the relatively unchanged vascularity and innervation. The pattern of musculus semitendinosus tendon structural changes seems to be connected to the individual homeostasis in patients persisting before the usage of tendon for the reconstruction.

A New Method Involving Percutaneous Application in the Treatment of Deep Venous Insufficiency: An Experimental Study With Internal Compression Therapy in a Porcine Model

2020 ◽  
pp. 153857442096611
Author(s):  
Alptekin Yasim ◽  
Erdinc Eroglu ◽  
Sezen Kocarslan ◽  
Atila Yoldas ◽  
Mehmet Acipayam ◽  
...  
Keyword(s):  
Side Effects ◽  
Connective Tissue ◽  
Alcian Blue ◽  
Venous Insufficiency ◽  
Porcine Model ◽  
Femoral Vein ◽  
Compression Therapy ◽  
Collagen I ◽  
Blue Staining ◽  
Genetic Examination

Introduction: To study the efficiency of internal compression therapy (ICT), a new and promising method of treatment for deep venous insufficiency, how that efficiency is achieved, and its potential side-effects, in a porcine model. Material and Methods: The femoral vein diameters of 4 pigs were first measured. ICT was then applied such as to reduce the diameter of these veins by 50%. The femoral vein diameters of 2 pigs were re-measured after 1 month. The femoral vein and its surrounding tissue were excised for immunohistopathological and genetic examination. The same procedures were applied to the remaining 2 pigs 3 months subsequently. Collagen I and IV immunohistochemical staining and Masson’s trichrome and Alcian blue histochemical staining were applied during immunohistopathological examination. Collagen I, III, and IV and connective tissue growth factor (CTGF) mRNA expressions were examined for genetic examination. Results: The femoral vein diameters decreased by approximately 50% after ICT application. This decrease persisted after the first and third months. Histopathological examination revealed loose connective tissue around the venous tissue after the operation, particularly in the third month, together with perivascular fibrosis and increased collagen in connective tissue. No difference was observed between regions with and without ICT application in terms of mucinous degeneration, an indicator of tissue injury, during Alcian blue staining. Genetic examination revealed an increase in collagen I and IV and CTGF mRNA expression in perivascular tissue resulting from ICT application. Conclusion: ICT is effective both in terms of creating a durable tissue around the vein and of increasing collagen tissue and stimulating fibrosis, and has no deleterious side-effects on tissue.

Prenatal undernutrition increases fat deposition and collagen content within skeletal muscle in the porcine fetus

2005 ◽  
Vol 2005 ◽  
pp. 37-37
Author(s):  
J. Karunaratne ◽  
C. Ashton ◽  
N.C. Stickland
Keyword(s):  
Skeletal Muscle ◽  
Connective Tissue ◽  
Meat Quality ◽  
Primary Objective ◽  
Fat Deposition ◽  
Collagen I ◽  
Ground Substance ◽  
Meat Industry ◽  
Pilot Studies ◽  
Fat Deposits

Connective tissue content of skeletal muscle plays a key role in meat quality. Previous pilot studies carried out in our lab have indicated that the smallest littermate may have a higher proportion of connective tissue in skeletal muscle (Clelland A., 2001). Connective tissue provides a structure to the muscle belly and is composed of ground substance, fibres and connective tissue cells. A proportion of these three elements of the connective tissue comprise of collagen I and fat deposits. This is an important concept to the meat industry as an increased amount of these components can increase meat toughness and intramuscular fat respectively, both having an impact on the resultant meat quality. The primary objective of this study is to investigate the relationship between undernutrition, collagen and fat content using a naturally occurring model. In the pig, it can be argued that differing levels of nutrition received, in utero, are a major cause of intra-litter variation. Therefore the smallest and largest littermates were chosen and content of collagen I and fat deposition were analysed in the M. semitendinosus of both.

scholarly journals Progressive Extracellular Matrix Disorganization in Chemically Induced Murine Oral Squamous Cell Carcinoma

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
B. Fuentes ◽  
J. Duaso ◽  
D. Droguett ◽  
C. Castillo ◽  
W. Donoso ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Connective Tissue ◽  
Cell Carcinoma ◽  
Basal Lamina ◽  
Squamous Cell ◽  
Collagen I ◽  
Severe Dysplasia ◽  
Tumor Pathogenesis

Introduction. Oral squamous cell carcinoma (OSCC) is one of the ten most common cancers affecting the human population. Tumor pathogenesis implies a multistep process in which cells acquire features that enable them to become tumorigenic and ultimately malignant. The process of OSCC carcinogenesis can be reproduced in animal models, the OSCC induction with 4-nitroquinoline-1-oxide (4NQO) in mice is a widely used tool for studying tumor pathogenesis. Objective. The aim of the present study was to determine the progressive changes in basal lamina and connective tissue remodeling during 4NQO-induced OSCC carcinogenesis. Material and Methods. Samples were classified according to “International Histological Classification of tumors” in mild, moderate, and severe dysplasia and invasive carcinoma. Five samples of each pathologic entity and control healthy tongues were used. Immunohistochemical analysis of collagen IV as well as histochemical analysis of glycosylated molecules (PAS) and collagen I (Picro Sirius red) were performed. Results. During experimental-induced carcinogenesis by 4NQO a progressive basal lamina destruction and collagen I disorganization in adjacent connective tissue can be observed. Conclusion. Our results confirm previous studies that show alterations in extracellular matrix (ECM) in malignant lesions, validating the experimental carcinogenesis induced by 4NQO.

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