Nutritional regulation of adipose tissue lipoprotein lipase is blunted in insulin resistant rats

2012 ◽  
Vol 7 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Monika Cahova ◽  
Zuzana Papackova ◽  
Eliska Palenickova ◽  
Helena Dankova ◽  
Jana Zdychova ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Nutritional Regulation ◽  
Insulin Resistant ◽  
Adipose Tissue Lipoprotein Lipase

Abstract

4.P.77 Adipose tissue lipoprotein lipase in insulin-resistant rats

1997 ◽  
Vol 134 (1-2) ◽  
pp. 312
Author(s):  
Teodoro Marotta ◽  
Martin Bergö ◽  
Thomas Olivecrona
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Insulin Resistant ◽  
Adipose Tissue Lipoprotein Lipase

scholarly journals Two different mechanisms are involved in nutritional regulation of lipoprotein lipase in guinea-pig adipose tissue

1989 ◽  
Vol 262 (2) ◽  
pp. 505-511 ◽  
Author(s):  
H Semb ◽  
T Olivecrona
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Guinea Pigs ◽  
Physiological State ◽  
Pulse Labelling ◽  
Nutritional Regulation ◽  
Lipoprotein Lipase Activity ◽  
Major Mechanism ◽  
Adipose Tissue Lipoprotein Lipase

Lipoprotein lipase activity in adipose tissue responds rapidly to changes in the physiological state. To study what mechanisms are involved in the regulation, guinea pigs were fasted and the decrease in adipose-tissue lipoprotein lipase activity was compared with the decreases in mRNA and lipase synthesis. The mRNA pattern (three species) did not change. There was a close parallelism between the abundance of lipase mRNA and relative lipase synthesis (immunoprecipitable 35S-labelled lipoprotein lipase as fraction of total [35S]protein after pulse-labelling with [35S]methionine). Total protein synthesis decreased on fasting, compounding the decrease in relative lipase synthesis. Lipoprotein lipase mRNA changed similarly in fat-pads and in isolated adipocytes, whereas lipase activity changed more in the pads, indicating disproportionally large changes in extracellularly located lipase. In old guinea pigs the decreases in lipoprotein lipase activity and lipase synthesis were comparable, but in young animals the change in lipase activity was substantially larger than the change in lipase synthesis. Refeeding of fasted young guinea pigs with glucose resulted in a rapid increase in lipoprotein lipase activity, but there was only a small change in lipase mRNA. Old animals responded slowly to refeeding. The results indicate that in older animals the major mechanism for regulation of adipose lipoprotein lipase activity is a relatively slow change in lipase mRNA, whereas in younger animals an additional, more rapid, regulation is exerted on the transport and turnover of the enzyme.

scholarly journals Post-translational regulation of lipoprotein lipase activity in adipose tissue

1978 ◽  
Vol 176 (3) ◽  
pp. 865-872 ◽  
Author(s):  
P Ashby ◽  
D P Bennett ◽  
I M Spencer ◽  
D S Robinson
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Translational Regulation ◽  
Progressive Increase ◽  
Lipoprotein Lipase Activity ◽  
Dependent Part ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Energy Dependent

Changes in adipose-tissue lipoprotein lipase activity that are independent of protein synthesis were investigated in an incubation system in vitro. Under appropriate conditions at 25 degrees C a progressive increase in the enzyme activity occurs that is energy-dependent. Part of the enzyme is rapidly inactivated when the tissue is incubated with adrenaline or adrenaline plus theophylline. The mechanism of this inactivation appears to be distinct from, and to follow, the activation of the enzyme. A hypothesis is presented to account for the results in terms of an activation of the enzyme during obligatory post-translational processing and a catecholamine-regulated inactivation of the enzyme as an alternative to secretion from the adipocyte.

Diurnal rhythms and effects of fasting and refeeding on rat adipose tissue lipoprotein lipase

1996 ◽  
Vol 271 (6) ◽  
pp. E1092-E1097 ◽  
Author(s):  
M. Bergo ◽  
G. Olivecrona ◽  
T. Olivecrona
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Specific Activity ◽  
Early Morning ◽  
Diurnal Rhythms ◽  
Mrna Levels ◽  
Short Term ◽  
Early Afternoon ◽  
Rat Adipose Tissue ◽  
Adipose Tissue Lipoprotein Lipase

The activity of lipoprotein lipase (LPL) in adipose tissue is modulated by changes in the nutritional status. We have measured LPL activity, mass, and mRNA levels in rat adipose tissue during normal feeding cycles, during short- and long-term fasting, and during refeeding after fasting. LPL activity displayed a diurnal rhythm. The activity was highest during the night and early morning, decreased to a minimum during the early afternoon, and then increased again. These changes corresponded to the feeding pattern. The increases and/or decreases resulted from changes in LPL synthetic rate compounded by posttranslational mechanisms. During short-term fasting, LPL specific activity decreased to < 30% of control. The specific activity was restored within 4 h by refeeding. On longer fasting, LPL mRNA decreased. This became significant from 36 h. On refeeding, it took 12 h to restore the mRNA levels, whereas tissue LPL activity and mass could not be fully restored by 36 h of refeeding. These data show that LPL activity during short-term fasting is regulated posttranscriptionally, which allows for quick upregulation after refeeding. On longer fasting, other mechanisms affecting LPL transcription and synthesis come into play, and upregulation after refeeding is slowed down.

Lipoprotein lipase activity in rat heart and adipose tissue during endotoxic shock

1980 ◽  
Vol 238 (3) ◽  
pp. H325-H330 ◽  
Author(s):  
G. J. Bagby ◽  
J. A. Spitzer
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Lipoprotein Lipase ◽  
Endotoxic Shock ◽  
Plasma Triglyceride ◽  
Lipoprotein Lipase Activity ◽  
Fat Pad ◽  
E Coli ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Observation Period

The present studies were designed to delineate changes in heart and adipose tissue lipoprotein lipase (LPL) activity following the administration of E. coli endotoxin. Plasma triglyceride levels were elevated in animals given endotoxin compared to saline-injected controls. Heart LPL activity decreased from 126.4 mumol fatty acid released per gram wet wt per hour in control rats to less than 22.5 mumol . g-1 . h-1 by 7 h following the injection of endotoxin. Although endotoxin was administered in doses producing 0-100% mortalities in a 24-h period, myocardial LPL activity was depressed to the same extent (75-80%) regardless of dose. The response of adipose tissue was less pronounced. Epididymal fat pad LPL activity fell significantly over the 24-h observation period in control and endotoxin-treated rats with the latter group somewhat more depressed 7 h after treatment. The findings are consistent with the suggestion that hypertriglyceridemia often observed during endotoxic shock may be related to depressed LPL activity; the degree of depression is probably tissue dependent.

scholarly journals Adipose-tissue lipoprotein lipase activity and cellularity in the genetically obese Zucker rat (fa/fa) (Short Communication)

1973 ◽  
Vol 132 (3) ◽  
pp. 633-635 ◽  
Author(s):  
P. de Gasquet ◽  
E. Péquignot ◽  
D. Lemonnier ◽  
A. Alexiu
Keyword(s):  
Adipose Tissue ◽  
Surface Area ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Enzyme Activities ◽  
Short Communication ◽  
Zucker Rat ◽  
Lipoprotein Lipase Activity ◽  
Obese Zucker Rat ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase activity per adipocyte was increased in the genetically obese rat (fa/fa). However, there was no difference between obese and lean animals when the enzyme activities were related to adipocyte surface area. The possible implications of the findings are discussed.

Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet

2002 ◽  
Vol 282 (2) ◽  
pp. E412-E418 ◽  
Author(s):  
Frédéric Picard ◽  
André Boivin ◽  
Josée Lalonde ◽  
Yves Deshaies
Keyword(s):  
Insulin Resistance ◽  
Lipoprotein Lipase ◽  
Sprague Dawley ◽  
High Fat ◽  
Insulin Resistant ◽  
Nutritional Conditions ◽  
Sprague Dawley Rats ◽  
Postprandial Hypertriglyceridemia ◽  
Adipose Tissue Lipoprotein Lipase ◽  
High Sucrose

This study aimed to assess whether adipose lipoprotein lipase (LPL) becomes resistant to insulin in a nutritional model of resistance of glucose metabolism to insulin. Sprague-Dawley rats were fed for 4 wk chow or a purified high-sucrose, high-fat (HSHF) diet that induced overt insulin resistance. Rats were fasted for 24 h and then refed chow for 1, 3, or 6 h. The postprandial rise in insulinemia was similar in both dietary cohorts, whereas glycemia was higher in HSHF-fed than in chow-fed animals, indicating glucose intolerance and insulin resistance. In chow-fed rats, adipose LPL activity increased two- to fourfold postprandially, but only minimally (30%) in HSHF-fed rats. Muscle LPL decreased postprandially in HSHF-fed rats, suggesting intact sensitivity to insulin, but it increased in chow-fed animals. Peak postprandial triglyceridemia was higher (+70%) in insulin-resistant than in control rats. The postprandial rate of appearance of triglycerides in the circulation was similar in control and insulin-resistant rats, indicating that hypertriglyceridemia of the latter was the result of impaired clearance. These results demonstrate that adipose LPL becomes resistant to insulin in diet-induced IR and further suggest that, under certain nutritional conditions, modifications in adipose LPL modulation associated with insulin resistance, along with low muscle LPL, heightens postprandial hypertriglyceridemia through attenuated triglyceride clearance.

Enhanced adipose tissue lipoprotein lipase activity in detrained rats: independent of changes in food intake

1994 ◽  
Vol 77 (6) ◽  
pp. 2564-2571 ◽  
Author(s):  
E. V. Lambert ◽  
G. Wooding ◽  
M. I. Lambert ◽  
J. H. Koeslag ◽  
T. D. Noakes
Keyword(s):  
Adipose Tissue ◽  
Food Intake ◽  
Exercise Training ◽  
Lipoprotein Lipase ◽  
Energy Intake ◽  
Body Mass ◽  
Exhaustive Exercise ◽  
Sedentary Control ◽  
Food Energy ◽  
Adipose Tissue Lipoprotein Lipase

Short-term detraining has been characterized by increased body mass and rapid body fat accretion. However, detraining has also been associated with increased food intake, especially in rats genetically predisposed to obesity. Thus, it has been difficult to separate refeeding effects from alterations resulting from the cessation of exercise training. In the present study, the in vitro activity of adipose tissue lipoprotein lipase (ATLPL) was measured in freely running wheel-trained rats and rats that had stopped training for 1, 2, or 3 days or 1 or 2 wk, respectively. Heparin-releasable ATLPL activity was measured at rest and after acute exhaustive exercise. Feeding efficiency (change in body mass/kJ ingested energy), fat pad mass, and adipocyte size were also measured. The rate of weight gain in 1- and 2-wk detrained rats was significantly higher than that of sedentary control or trained rats (P < 0.05). Feeding efficiency was also higher in 1-wk detrained rats than in all other groups (P < 0.005). However, food energy intake was not different between trained rats, 1- and 2-wk detrained rats, or sedentary control rats. ATLPL activity in all groups was highest after acute exhaustive exercise. ATLPL activity in 1-wk detrained rats was nearly threefold higher compared with that in trained rats (P < 0.005) and was not different from that of sedentary control rats. These results suggest that the cessation of exercise training causes an enhanced capacity for lipogenesis independent of changes in food energy intake or the acute effects of the last bout of exercise.

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