Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa

AbstractThe present study describes the total phenolic content, concentrations of flavonoids and in vitro antioxidant and antimicrobial activity of methanol extracts from Seseli pallasii Besser, S. libanotis (L.) Koch ssp. libanotis and S. libanotis (L.) Koch ssp. intermedium (Rupr.) P. W. Ball, growing wild in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 84.04 to 87.52 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 4.75 to 19.37 mg Qu (quercetin)/g. Antioxidant activity was analyzed using DPPH reagent. Antioxidant activity ranged from 0.46 to 4.63 IC50 (mg/ml) and from 1.98 to 2.19 mg VitC (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively, using BHA and VitC as controls. The antimicrobial activity of the extracts was investigated using a micro-well dilution assay for the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. This finding suggests that Seseli species may be considered as a natural source of antioxidants and antimicrobial agents.

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Pharmacognostical, Phytochemical, Antioxidant and Antimicrobial Activity of Costus woodsonii

Edelweiss Applied Science and Technology ◽

10.33805/2576-8484.168 ◽

2019 ◽

pp. 53-59

Author(s):

Mukesh S Sikarwar ◽

Chew Khai Szeek ◽

Neeraj Paliwal

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Plant Extract ◽

Total Phenolic Content ◽

Leaf Extract ◽

Phenolic Content ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Concentration Test ◽

Antioxidant And Antimicrobial Activity

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.

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Total phenolic content, antioxidant and antimicrobial activity of Equisetum arvense L.

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120327040k ◽

2013 ◽

Vol 19 (1) ◽

pp. 37-43 ◽

Cited By ~ 4

Author(s):

Zoran Kukric ◽

Ljiljana Topalic-Trivunovic ◽

Svetlana Pavicic ◽

Mirjana Zabic ◽

Snjezana Matos ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Activity Index ◽

Total Phenolic ◽

Bacterial Suspension ◽

Equisetum Arvense ◽

Antioxidant And Antimicrobial Activity ◽

The Impact

The antioxidant and antimicrobial activity of Equisetum arvense L. harvested in a polluted urban area were investigated. The total phenolic content determined by a modified Folin-Ciocalteu method was 335.8 mg of GEA/g of the dried ethanol extract. Antioxidant activity determined by FRAP method was 28.7 mM Fe(II)/g of extract, whereas antioxidant activity index for stable DPPH free radicals was 3.86. The results were compared with control antioxidants (gallic acid, vitamin C, BHA and BHT). Antimicrobial activity was tested by monitoring the impact of different extract concentrations through measuring the change in optical density of bacterial suspension over time and by determining MIC and MBC using macrodilution method. E. arvense extract has been effective on the tested microorganisms depending on the concentration and exposure time. The extract had the strongest antibacterial activity on Staphylococcus aureus, with MIC and MBC of 11.14 mg/mL and 22.28 mg/mL respectively, and the weakest effect on Bacillus cereus, with MIC of 89.10 mg/mL.

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Total Phenolic Content, Antioxidant and Antimicrobial Activity ofSambacus nigraL

Journal of Biologically Active Products from Nature ◽

10.1080/22311866.2012.10719135 ◽

2012 ◽

Vol 2 (5) ◽

pp. 275-283 ◽

Cited By ~ 1

Author(s):

Mohadese Mahboubi ◽

Nastaran Kazempour ◽

Atefeh Mahboubi

Keyword(s):

Antimicrobial Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Antioxidant And Antimicrobial Activity

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Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species

Open Life Sciences ◽

10.2478/s11535-012-0048-x ◽

2012 ◽

Vol 7 (4) ◽

pp. 664-671 ◽

Cited By ~ 4

Author(s):

Milan Stanković ◽

Olgica Stefanović ◽

Ljiljana Čomić ◽

Marina Topuzović ◽

Ivana Radojević ◽

...

Keyword(s):

Antimicrobial Activity ◽

Total Phenolic Content ◽

Antimicrobial Agents ◽

Phenolic Content ◽

Fungal Species ◽

Total Phenolic ◽

Total Phenols ◽

Further Development ◽

Ethyl Acetate Extracts

AbstractIn vitro antimicrobial activity of 21 crude extracts obtained from seven taxa of the genus Teucrium (T. chamaedrys, T. montanum, T. arduini, T. polium, T. scordium subsp. scordium, T. scordium subsp. scordioides and T. botrys) was tested against bacterial and fungal species. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using a microdilution analysis method. Total phenolic content and flavonoid concentrations were measured spectrophotometrically. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 28.49 up to 159.84 mg CA/g of extract (chlorogenic acid equivalent). The amounts of flavonoids ranged from 38.17 up to 190.45 mg RU/g of extract (rutin equivalent).The plant extracts showed greater potential of antibacterial than antifungal activity. A relationship was found between total phenolics and biological activity. The highest level of total phenols was measured in the methanol extracts, which demonstrated higher antimicrobial activity than acetone and ethyl acetate extracts. Staphylococcus aureus ATCC 25923 appeared to be the most sensitive organism. Our results indicate that Teucrium spp extracts are rich sources of phenolic compounds and are promising candidates for further development as natural antimicrobial agents.

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COMPARATIVE EVALUATION OF THE ANTIOXIDANT POTENTIAL OF Hericium erinaceus, Hericium americanum AND Hericium coralloides

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2019.6.10 ◽

2019 ◽

Vol 18 (6) ◽

pp. 97-105

Author(s):

Funda Atila

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Total Phenolic ◽

Antioxidant Power ◽

Hericium Erinaceus ◽

Methanol Extracts ◽

Ferric Reducing Antioxidant Power ◽

High Antioxidant Activity

The aim of this work was to determine the total phenolic content (TPC) and the antioxidant activity of methanol extracts of Hericium erinaceus, Hericium americanum and Hericium coralloides, including free radical scavenging method (DPPH), ferric reducing antioxidant power (FRAP) and radical cation scavenging method (ABTS). Hericium spp exhibited moderate to high antioxidant activity. The highest TPC (3.27 ±0.01 mg GAE g–1) and antioxidant activity values (17.0 ±0.68 mmol TE g‒1 in FRAP; EC50 = 4.12 ±0.12 mg mL‒1 in DPPH•; EC50 = 2.83 ± 0.10 mg mL‒1 in ABTS•+) were found for methanol extracts of H. coralloides. The TPC and antioxidant activity of H. erinaceus isolates varied from strain to strain. H. americanum possessed considerably lower total phenolic content (2.31 ±0.01 mg GAE g‒1) and antioxidant activity (10.5 ±0.59 mmol TE g‒1 in FRAP; EC50 = 7.82 ±0.09 mg mL‒1 in DPPH•; EC50 = 6.36 ± 0.12 mg mL‒1 in ABTS•+) than H. coralloides and H. erinaceus. A high correlation was determined between TPC and ABTS•+ (r2 = 0.855), DPPH• (r2 = 0.969) and FRAP (r2 = 0.942). According to results obtained in the present study, Hericium spp., especially H. coralloides and some of H. erinaceus isolates, might be promising natural source of antioxidants for food and pharmaceutical industry.

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Determination of Phenolic Content, Ascorbic Acid, Antioxidant Activity and Antimicrobial Activity of Selected Fruit Waste

International Journal of Engineering & Technology ◽

10.14419/ijet.v7i4.14.27578 ◽

2019 ◽

Vol 7 (4.14) ◽

pp. 263 ◽

Cited By ~ 1

Author(s):

Syeril Nurfatihah Suhaimi ◽

Erniee Eileen Rizlan Ross ◽

Ishak Zubir ◽

Shanti Navaratnam ◽

Raseetha Siva Siva

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Banana Peel ◽

Total Phenolic ◽

Zone Of Inhibition ◽

Pineapple Peel ◽

Cocoa Pod Husk

The aim of this study was to determine the total phenolic content (TPC), antioxidant activity, ascorbic acid content and antimicrobial activity of extracts obtained from cocoa pod husk, banana peel and pineapple peel. Banana peel had significantly highest total phenolic content (154.50 mg GAE/g) followed by pineapple peel (140.37 mg GAE/g) and cocoa pod husk (114.08 mg GAE/g). Antioxidant activity of these samples measured using DPPH assays. Banana peel showed significantly higher DPPH scavenging activity (95.74%) compared to pineapple peel (84.96%) and cocoa pod husk (68.33%). Pineapple peel resulted in significantly higher (44.19 ppm) ascorbic acid as measured using High performance liquid chromatography (HPLC) method compared to banana peel (28.56 ppm). Cocoa pod husk, banana peel and pineapple peel were observed for antimicrobial activity against Escherichia coli, Staphylococcus aureus and penicillium. Samples extract at different concentrations in E. coli, S.aureus and penicillium-seeded Mueller-Hinton agar medium, resulted zone of inhibition after 24 h incubation in 37°C for bacteria and 72 h incubation in 28ºC. Banana peel at 20 and 25mg/ml against S.aureus resulted in zone of inhibition 9.67, 11.67 mm and cocoa pod husk with 8.00, 9.67 mm respectively. Cocoa pod husk at 15, 20 and 25mg/ml against E.coli resulted in zone of inhibition 7.33, 9.33 and 10.33 mm and banana peel with 6.67, 7.33 and 7.67 mm respectively. Pineapple peel does not showed any inhibition zone against tested bacteria and fungi.

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Antioxidant and Antimicrobial Evaluation and Chemical Investigation of Rosa gallica var. aegyptiaca Leaf Extracts

Molecules ◽

10.3390/molecules26216498 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6498

Author(s):

Ahmed S. Abdelbaky ◽

Abir M. H. A. Mohamed ◽

Salman S. Alharthi

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Phenolic Compounds ◽

Total Phenolic Content ◽

Phenolic Content ◽

Flowering Plant ◽

Diffusion Method ◽

Total Phenolic ◽

Leaf Extracts ◽

Rosa Gallica

Rosa gallica var. aegyptiaca is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of Rosa gallica var. aegyptiaca leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including Listeria monocytogenes, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis—and the fungus Candida albicans, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-O-α-d-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of R. gallica var. aegyptiaca leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.

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Comparison of biochemical and antimicrobial activities of different honey samples

Czech Journal of Food Sciences ◽

10.17221/38/2021-cjfs ◽

2021 ◽

Vol 39 (No. 4) ◽

pp. 273-280

Author(s):

Saban Keskin ◽

Semiramis Karlidag ◽

Nazlı Mayda ◽

Asli Ozkok

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Biological Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Biological Activities ◽

Total Phenolic ◽

Honey Sample ◽

Gram Positive ◽

Gram Positive Bacteria

Honey, a natural healing agent and a sweet food, has been used since ancient times. A honey sample could possess many biological activities depending on its chemical composition. The amount and the diversity of these minor components of honey mainly depend on the floral sources. That is why the biological activity of the honey sample obtained in a region should be determined. In this study, total phenolic and flavonoid content, antioxidant activity, melissopalynological analyses and antimicrobial activity of twenty honey samples obtained from Doganyol, Malatya, Turkey were examined. In order to determine the in vitro antibacterial activity of honey samples, the agar well diffusion (AWD) method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were used. For this purpose, ten Gram-positive bacteria and eight Gram-negative bacteria were used. Total phenolic content was found in the range from 9.68 ± 0.72 to 29.40 ± 1.03 mg GAE g–1 sample. Antioxidant activity of honey samples varied from 2.21 ± 0.46 to 6.03 ± 1.11 µmol FeSO4 7H2O g–1. Honey samples showed moderate antimicrobial activity against tested microorganisms, especially against Gram-positive bacteria. It could be concluded from our findings that there is a good correlation between total phenolic content and the biological activity of honey samples.

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Radical Scavenging Activity and Total Phenolic Content of Extracts of the Root Bark of Osyris Lanceolata

Natural Product Communications ◽

10.1177/1934578x0900400120 ◽

2009 ◽

Vol 4 (1) ◽

pp. 1934578X0900400

Author(s):

Elizabeth M. O. Yeboah ◽

Runner R. T. Majinda

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Total Phenolic ◽

Root Bark ◽

Fast Kinetics ◽

Methanol Extracts ◽

Slow Kinetics

The root bark of Osyris lanceolata was screened for its antioxidant potential using three variations of the DPPH radical scavenging method viz a TLC-autographic assay, a semi-quantitative TLC assay, and a spectrophotometric assay. The total phenolic content was evaluated as gallic acid equivalents (GAE) using the Folin-Ciocalteu method. The powdered root bark was extracted sequentially with n-hexane, chloroform, methanol and 90% methanol/water. A separate supercritical fluid extraction (SFE) of the root bark was also carried out. The radical scavenging ability, as measured by IC50 values, was found to be 48.4 ± 0.4 and 49.5 ± 1.9 μg/mL for the 90% methanol/water and methanol extracts respectively, while those of the chloroform, n-hexane, and SFE extracts were found to be < 250 μg/mL. Under the same conditions, the values for the standards, ascorbic acid and gallic acid, were found to be 38.70 ± 0.08 and 2.86 ± 0.01 μg/mL respectively. The radical scavenging power of the five extracts correlated positively with the total phenolic content and was ranked in the following decreasing order: 90% methanol/water > methanol > chloroform > SFE > n-hexane with total phenolic contents of 290.2 ± 3.3, 271.0 ± 3.7, 74.4 ± 0.8, 62.5 ± 0.4 and 49.5 ± 1.9 mg GAE/g of dry extract, respectively. The 90% methanol/water and methanol extracts showed several components with high antioxidant activity displaying fast kinetics in both TLC assays, while the chloroform, SFE, and n-hexane extracts exhibited slow kinetics antioxidant activity.

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