scholarly journals Effects of powdery mildew severity (Blumeria graminis f. sp. tritici) on breeding lines of durum wheat (Triticum turgidum L. spp. durum) yield in Western Andalusia [Spain]

2003 ◽  
Vol 1 (3) ◽  
pp. 13 ◽  
Author(s):  
M.M. Cátedra Cerón ◽  
I. Solís Martel
2004 ◽  
Vol 84 (4) ◽  
pp. 1001-1013
Author(s):  
J. E. Dexter ◽  
M. A. Doust ◽  
C. N. Raciti ◽  
G. M. Lombardo ◽  
F. R. Clarke ◽  
...  

Since the 1980s, there have been general trends in the durum wheat milling industry to higher semolina extraction rate, and in the pasta processing industry to the use of higher drying temperatures. During this time, specification of gluten strength by gluten index, mixograph mixing properties and alveograph parameters has also become widespread. These trends prompted this study of the appropriateness of protocols for quality testing of Canadian durum wheat breeding lines. Four cultivars with intrinsic differences in yellow pigment levels and gluten strength were grown in field plots in Swift Current, Saskatchewan for three consecutive years. A laboratory-scale milling procedure was modified to produce semolina at extraction rates from about 65% to about 80%. Milling to extraction rates above 65%, the extraction rate used routinely in quality testing of Canadian durum wheat breeding lines, had a major impact on semolina ash content and colour, but did not offer any advantage in ranking cultivars for either semolina yield or semolina refinement. Gluten strength, as measured by gluten index, was independent of semolina extraction rate. Dough strength, as measured by mixograph properties and alveograph properties, showed a tendency to weakening at high extraction, particularly for strong cultivars. Semolina was processed into spaghetti using low-temperature (LT), high-temperature (HT) and ultra-high-temperature (UHT) drying cycles. The firmness of cooked spaghetti was predominantly influenced by protein content. As a result, cultivars generally ranked in spaghetti firmness according to protein content. Regardless of drying cycle or cultivar, spaghetti firmness increased as drying temperature increased. Spaghetti dried at LT was less yellow than spaghetti dried at HT or UHT, probably due to thermal inactivation of the bleaching enzyme lipoxygenase at HT and UHT. Regardless of drying cycle, spaghetti became duller, more red and less yellow as extraction rate increased. For each spaghetti trait, cultivar ranking remained relatively constant regardless of extraction rate or drying temperature. On the basis of these results, there appears to be no advantage to increasing semolina extraction rate beyond 65% for evaluation of durum wheat milling performance, gluten strength or pasta properties. In addition, it appears that one drying cycle is adequate to reliably evaluate durum wheat lines for spaghetti colour and firmness. Key words: Durum wheat (Triticum turgidum L. var durum), milling, semolina, pasta, quality screening, gluten strength, colour, texture


Genome ◽  
2008 ◽  
Vol 51 (12) ◽  
pp. 1016-1025 ◽  
Author(s):  
S. Reimer ◽  
C. J. Pozniak ◽  
F. R. Clarke ◽  
J. M. Clarke ◽  
D. J. Somers ◽  
...  

Association mapping (AM) is an alternative or complementary strategy to QTL mapping for describing associations between genotype and phenotype based on linkage disequilibrium (LD). Yellow pigment (YP), an important end-use quality trait in durum wheat (Triticum turgidum L. var. durum), was evaluated to determine the ability of AM to identify previously published QTL and to identify genomic regions for further genetic dissection. The YP concentration was determined for 93 durum wheat accessions sampled from a variety of geographic origins. Analysis of population structure using distance- and model-based estimates indicated the presence of five subpopulations. Using subpopulation assignments as covariates, significant (P < 0.05) marker–trait associations for YP were detected on all chromosomes of the durum genome. Using AM, genomic regions housing known YP QTL were confirmed, most notably the group 7 chromosomes. In addition, several markers on the group 1, 2, and 3 chromosomes were identified where QTL have yet to be reported. A phytoene synthase gene, Psy1-B1, a potential candidate gene for YP, was significantly associated with YP and was in strong LD with microsatellite markers on the distal end of 7BL. Our results demonstrated that AM complemented traditional QTL mapping techniques and identified novel QTL that should be the target of further genetic dissection.


Plant Disease ◽  
2002 ◽  
Vol 86 (4) ◽  
pp. 383-388 ◽  
Author(s):  
Yasmina Imani ◽  
Abdallah Ouassou ◽  
Carl A. Griffey

The incidence and severity of powdery mildew, caused by Blumeria graminis f. sp. tritici, have increased in Morocco during the past decade as a result of the introduction and intensive production of a few semidwarf cultivars of bread wheat (Triticum aestivum) and durum wheat (Triticum turgidum) that are genetically uniform, and the increased utilization of nitrogen fertilizers and irrigation. The virulence of the pathogen population has not been characterized in Morocco, and little is known about the spectra and distribution of virulence in B. graminis populations. Such knowledge will facilitate the identification and utilization of effective resistance genes in cultivar development and deployment. Isolates of B. graminis collected in 1999 and 2000 from three Moroccan wheat production areas were analyzed for virulence using a host differential series comprised of 13 known genes conferring resistance to powdery mildew. Segments of primary leaves from 12-day-old seedlings of the wheat differentials were inoculated with isolates of B. graminis derived from a single pustule. Powdery mildew reactions were assessed on a 0 to 9 scale 12 days after inoculation. Virulence frequencies, complexity, and racial composition of the pathogen population were determined. Data were analyzed for associations among pairs and triplets of virulence genes and for distribution of virulence genes among pathotypes. High frequencies of virulence to genes Pm1, Pm3c, Pm3f, Pm4a, pm5, and Pm7 were found over both years and across all three regions. Frequencies of virulence for Pm17 and Pm2 were intermediate, while virulence frequencies for Pm3a, Pm3b, Pm3d, and Pm4b were low. Virulence to Pm8 increased to high levels, while virulence to Pm4a decreased across the area surveyed from 1999 to 2000. The random distribution of virulence genes among patho-types indicates that sexual reproduction contributes to the variability of the pathogen. The Moroccan population of Blumeria graminis f. sp. tritici is composed of complex and highly variable pathotypes (87% of the isolates had five or more virulence genes), and strategies for deployment of resistance genes should take into account this complexity.


Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 391-397 ◽  
Author(s):  
Abdelrazek Abdelrhim ◽  
Harby M. Abd-Alla ◽  
El-Sayed Abdou ◽  
Mamdoh E. Ismail ◽  
Christina Cowger

Powdery mildew, caused by Blumeria graminis (DC.) Speer f. sp. tritici (Em. Marchal), is a serious disease of wheat that can cause a large reduction in yield. In Egypt, high powdery mildew severity has been observed in the past few years on many commercial cultivars of both bread and durum wheat. Little information is available about virulence characteristics of the Egyptian B. graminis f. sp. tritici population in Egypt or the resistance of Egyptian wheat cultivars to powdery mildew. Virulence frequencies of a representative sample of the Egyptian B. graminis f. sp. tritici population were studied. Seven provinces were chosen to represent the country: two in Upper Egypt (Qena and Sohag), one in Middle Egypt (El Minia), and four in the north (Alexandria, Kafr Elsheikh, Dakahlia, and Sharqia). Ten isolates from each province (70 isolates total) were derived from single ascospores and used for this study. They were inoculated individually on 21 powdery mildew differential lines, each bearing a single resistance (Pm) gene. Also, the responses of 14 Egyptian bread wheat cultivars and 6 durum cultivars to each of the 70 isolates were evaluated individually. Among all tested Pm genes, only seven (Pm1b, Pm2, Pm21, Pm34, Pm36, Pm37, and Pm53) were effective against B. graminis f. sp. tritici isolates from all provinces. Several other genes were effective against most or all isolates from a majority of provinces. All tested bread wheat cultivars showed full susceptibility to all isolates, whereas two durum wheat cultivars, Beni-Suef-5 and Beni-Suef-6, had intermediate responses to a large percentage of the isolates, likely indicating partial resistance. To enhance mildew resistance in Egyptian wheat cultivars, it is recommended to use combinations of genes that are nationally effective or effective against multiple provincial B. graminis f. sp. tritici populations.


Author(s):  
Rosa Cifarelli ◽  
Olimpia D’Onofrio ◽  
Rosalba Grillo ◽  
Teresa Mango ◽  
Francesco Cellini ◽  
...  

AbstractTotipotent cDNA libraries representative of all the potentially expressed sequences in a genome would be of great benefit to gene expression studies. Here, we report on an innovative method for creating such a library for durum wheat (Triticum turgidum L. var. durum) and its application for gene discovery. The use of suitable quantities of 5-azacytidine during the germination phase induced the demethylation of total DNA, and the resulting seedlings potentially express all of the genes present in the genome. A new wheat microarray consisting of 4925 unigenes was developed from the totipotent cDNA library and used to screen for genes that may contribute to differences in the disease resistance of two near-isogenic lines, the durum wheat cultivar Latino and the line 5BIL-42, which are respectively susceptible and resistant to powdery mildew. Fluorescently labeled cDNA was prepared from the RNA of seedlings of the two near-isogenic wheat lines after infection with a single powdery mildew isolate under controlled conditions in the greenhouse. Hybridization to the microarray identified six genes that were differently expressed in the two lines. Four of the sequences could be assigned putative functions based on their similarity to known genes in public databases. Physical mapping of the six genes localized them to two regions of the genome: the centromeric region of chromosome 5B, where the Pm36 resistance gene was previously localized, and chromosome 6B.


2012 ◽  
Vol 10 (3) ◽  
pp. 165-170 ◽  
Author(s):  
Khola Rafique ◽  
Awais Rasheed ◽  
Alvina Gul Kazi ◽  
Hadi Bux ◽  
Farah Naz ◽  
...  

Triticum urartu possesses the Au genome common to bread wheat. Similarly, Triticum monococcum contains the Am genome, which is closely related to the A-genome donor of bread wheat. Aegilops speltoides of the Sitopsis section has the S genome, which is most similar to the B genome of bread and durum wheat when compared with all other wild grasses. Amphiploids developed through bridge crossing between Am/Au and S-genome diploid resources and elite durum cultivars demonstrate enormous diversity to improve both bread and durum wheat cultivars. We evaluated such A-genome amphiploids (Triticum turgidum × T. urartu and T. turgidum × T. monococcum, 2n = 6x = 42; BBAAAmAm/AuAu) and S-genome amphiploids (T. turgidum × Ae. speltoides, 2n = 6x = 42; AABBSS) along with their durum parents (AABB) for their resistance to powdery mildew (PM) at the seedling stage. The results indicated that 104 accessions (53.6%) of A-genome amphiploids (AABBAmAm/AuAu) were resistant to PM at the seedling stage. Of their 24 durum parents, five (20.83%) were resistant to PM and 16 (66.6%) were moderately tolerant. Similarly, ten (50%) accessions of S-genome amphiploids (BBAASS) possessed seedling PM resistance, suggesting a valuable source of major resistance genes. PM screening of the amphiploids and parental durum lines showed that resistance was contributed either by the diploid progenitors or durum parents, or both. We also observed the suppression of resistance in several cases; for example, resistance in durum wheat was suppressed in respective amphiploids. The results from this germplasm screening will facilitate their utilization to genetically control PM and widen the genetic base of wheat.


2020 ◽  
Vol 21 (21) ◽  
pp. 7910
Author(s):  
Rosanna Simeone ◽  
Luciana Piarulli ◽  
Domenica Nigro ◽  
Massimo Antonio Signorile ◽  
Emanuela Blanco ◽  
...  

Wheat is the most widely grown crop and represents the staple food for one third of the world’s population. Wheat is attacked by a large variety of pathogens and the use of resistant cultivars is an effective and environmentally safe strategy for controlling diseases and eliminating the use of fungicides. In this study, a collection of wild and cultivated tetraploid wheats (Triticum turgidum) were evaluated for seedling resistance (SR) and adult plant resistance (APR) to powdery mildew (Blumeria graminis) and genotyped with a 90K single nucleotide polymorphism (SNP) array to identify new sources of resistance genes. The genome-wide association mapping detected 18 quantitative trait loci (QTL) for APR and 8 QTL for SR, four of which were identical or at least closely linked to four QTL for APR. Thirteen candidate genes, containing nucleotide binding sites and leucine-rich repeats, were localized in the confidence intervals of the QTL-tagging SNPs. The marker IWB6155, associated to QPm.mgb-1AS, was located within the gene TRITD1Av1G004560 coding for a disease resistance protein. While most of the identified QTL were described previously, five QTL for APR (QPm.mgb-1AS, QPm.mgb-2BS, QPm.mgb-3BL.1, QPm.mgb-4BL, QPm.mgb-7BS.1) and three QTL for SR (QPm.mgb-3BL.3, QPm.mgb-5AL.2, QPm.mgb-7BS.2) were mapped on chromosome regions where no resistance gene was reported before. The novel QTL/genes can contribute to enriching the resistance sources available to breeders.


2012 ◽  
Vol 92 (5) ◽  
pp. 973-975 ◽  
Author(s):  
Lily Tamburic-Ilincic ◽  
Arend Smid ◽  
Carl Griffey

Tamburic-Ilincic, L., Smid, A. and Griffey, C. 2012. OAC Amber winter durum wheat. Can. J. Plant Sci. 92: 973–975. OAC Amber is the first winter durum wheat (Triticum turgidum subsp. durum L.) cultivar registered for Ontario, Canada. It is an awned wheat with amber colored kernels, high test weight, kernel weight, and protein level with good winter hardiness. OAC Amber has good resistance to leaf rust (Puccinia triticina) but is moderately susceptible to powdery mildew (Blumeria graminis) and leaf blotch (Septoria tritici), and susceptible to Fusarium head blight (FHB). OAC Amber is well adapted for the winter wheat growing areas of Ontario.


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