First detection and genotyping of human-associated microsporidia in wild waterfowl of Slovakia

2013 ◽  
Vol 58 (1) ◽  
Author(s):  
B. Malčeková ◽  
A. Valenčáková ◽  
L. Molnár ◽  
A. Kočišová

AbstractA total of 47 avian faecal samples of wild waterfowl (great cormorant — Phalacrocorax carbo, great crested grebe — Podiceps cristatus, white stork — Ciconia ciconia) trapped in the eastern Slovakia were screened for the presence of human pathogenic microsporidia by microscopy and real-time SYBR Green PCR method using species primers and sequenced. Microscopic analysis showed presence in 32 samples (29 cormorants, 3 dippers). Microsporidial DNA (Encephalitozoon cuniculi genotype I) was identified in 19 faeces samples (40.4%) namely cormorants in 17 out of 40, one dipper of 5 and a stork out of 2. The present work describes three new host species of the bird population in microsporidium Encephalitozoon cuniculi genotype I which confirms the theory of low specificity of this species.

Zootaxa ◽  
2011 ◽  
Vol 2817 (1) ◽  
pp. 63 ◽  
Author(s):  
ELIZA GLOWSKA ◽  
MACIEJ SKORACKI

In this paper we describe a new quill mite species Stibarokris dastychi sp. nov., parasitizing Great Cormorant Phalacrocorax carbo (Linnaeus) (Pelecaniformes: Phalacrocoracidae) and the male of S. phoeniconaias Skoracki et OConnor, 2010 from American Flamingo Phoenicopterus ruber Linnaeus (new host). Additionally, the improved (including both female and male) diagnosis of the genus Stibarokris Kethley, 1970 and the key to all known species of this genus are given.


ARCTIC ◽  
2016 ◽  
Vol 69 (1) ◽  
pp. 29 ◽  
Author(s):  
Carla J. Dove ◽  
Stephen Wickler

A grave containing the remains of a wooden boat was discovered in 1934 under a low mound in a bog at Øksnes in the Vesterålen islands of northern Norway. The boat grave dates to the 10th century in the Viking Age, and grave goods placed in the boat include an iron axe, a cowhide in which the body was wrapped, and pillow remains consisting of feather stuffing and a wool textile cover. A microscopic analysis of the feathers from a subsample of the pillow fill identified three avian orders: Anseriformes (eider); Suliformes (cormorant), and Charadriiformes (unspecified gull). It was possible to make one species-level identification of Great Cormorant (<em>Phalacrocorax carbo</em>) and to narrow the gull types to the “white-headed” gull group. The sample was composed of a nearly equal mix of downy and pennaceous feather types. Downy feathers from gulls (Laridae) composed the majority of the material in this sample. While it is reported that feathers and down (assumed to be eider) were used in the Late Iron Age, this is the first successful attempt to identify bird species used in these materials and suggests that avian species identifications should be explored in other such burial items to enhance our understanding of human-wildlife interactions throughout Norse history.


2014 ◽  
Vol 3 ◽  
Author(s):  
K. R. Kerr ◽  
S. E. Dowd ◽  
K. S. Swanson

AbstractThere has been a recent increase in the feeding of unconventional diets, including whole-prey diets, to domestic pet cats. Our objective was to characterise faecal microbial populations of domestic cats fed whole and ground (6·35 mm grind) raw 1–3-d-old chicks (Rodent Pro). Faecal samples were collected from neutered male domestic cats (mean age = 5·7 years) fed these diet items in a crossover design. Bacterial DNA was isolated from faecal samples and amplicons of the 16S rRNA V4–V6 region were generated and analysed by 454 pyrosequencing. Faecal microbial populations of cats fed whole v. ground chicks did not differ. During the study, three cats presented with symptoms of infection (anorexia or diarrhoea) and tested clinically positive for Salmonella using a standard PCR method. The remaining cats tested negative. Data were analysed post hoc to test for differences in microbial populations due to clinical status. The predominant genera were Clostridium (9–30 %), unidentified Lachnospiraceae (10–28 %), Blautia (4–19 %), Peptococcus (2–19 %) and Fusobacterium (2–14 %). Faeces of cats testing clinically positive for Salmonella had higher (P ≤ 0·05) proportions of the genera Coprococcus (5·6 v. 0·4 %) and Escherichia (subgenera Shigella; 1·1 v. 0·3 %). Salmonella was not detected in faecal samples utilising the pyrosequencing method; however, there was a shift in microbial populations due to clinical status. The clinical symptoms reported herein may be not only due to the Salmonella itself, but also shifts in other gut microbial populations.


2018 ◽  
Vol 117 (11) ◽  
pp. 3663-3667 ◽  
Author(s):  
Petras Prakas ◽  
Dalius Butkauskas ◽  
Saulius Švažas ◽  
Vitas Stanevičius

2015 ◽  
Vol 90 (4) ◽  
pp. 392-397 ◽  
Author(s):  
W. Hu ◽  
X.G. Yu ◽  
S. Wu ◽  
L.P. Tan ◽  
M.R. Song ◽  
...  

AbstractAncylostoma ceylanicum is a common zoonotic nematode. Cats act as natural reservoirs of the hookworm and are involved in transmitting infection to humans, thus posing a potential risk to public health. The prevalence of feline A. ceylanicum in Guangzhou (South China) was surveyed by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). In total, 112 faecal samples were examined; 34.8% (39/112) and 43.8% (49/112) samples were positive with hookworms by microscopy and PCR method, respectively. Among them, 40.8% of samples harboured A. ceylanicum. Twelve positive A. ceylanicum samples were selected randomly and used for cox 1 sequence analysis. Sequencing results revealed that they had 97–99% similarity with A. ceylanicumcox 1 gene sequences deposited in GenBank. A phylogenetic tree showed that A. ceylanicum isolates were divided into two groups: one comprising four isolates from Guangzhou (South China), and the other comprising those from Malaysia, Cambodia and Guangzhou. In the latter group, all A. ceylanicum isolates from Guangzhou were clustered into a minor group again. The results indicate that the high prevalence of A. ceylanicum in stray cats in South China poses a potential risk of hookworm transmission from pet cats to humans, and that A. ceylanicum may be a species complex worldwide.


2016 ◽  
Vol 24 (2) ◽  
Author(s):  
A. I. Sidorenko ◽  
V. D. Siokhin

In Ukraine the Great Cormorant (Phalacrocorax carbo Linnaeus, 1758) uses a rather wide range of habitats for nesting: islands, trees and shrubs, reedbeds and a variety of man-made structures. In general, the strategy of nesting on man-made structures is uncommon both in Ukraine and Europe, and Cormorantsdo this only in the absence of other sites suitable for nesting. Special research onCormorant colonies on technogenic constructions was carried out during the field expeditions by the Research Institute of Biodiversity of Terrestrial and Aquatic Ecosystems of Ukraine in 2002–2003 and 2012–2016. Besides this, we used retrospective and current data from the literature and Internet resources. Most of the field work was carried out by making surveys by boat and on foot. As a result, we found 8 Cormorant colonies on technogenic constructions in Ukraine: gas platforms in the Sea of Azov (near the village Strilkove, Henichesk district of Kherson region); sunken ships – targets for bombing training near the Arabat Spit (these are also known as «ship islands»); electricity pylons of the high-voltage Enerhodar Dnipro Power Line where it crosses the Kakhovka Reservoir; the dock in Yahorlyk Bayk, used in the past as a target for bombing training bombing; artificial island-platforms on Lake Chernine (Kinburn Peninsula); an artificial island on the Sasyk Lagoon (Odessa region); artificial islands, made as navigation markers on the Kremenchuk and Kiev reservoirs. The study found that in most cases the accompanying species was the CaspianGull (Larus cachinnans Pallas, 1811), which actively destroys the Cormorants’ nests and eats their eggs and chicks. The number of nests in the colonies varied greatly (5–30 nests on the navigation marker islands and ca. 2 000–2 300 on the «ship-islands» and gas platforms). This is due, primarily, to the area of the breeding territory. The research found that fierce territorial competition was observed in most of the colonies both with Caspian Gulls and between Cormorants. In addition, we observed anthropogenic interference in the colonies by fishermen and workers conducting routine maintenance work (as happened in the case of electricity pylons and gas platforms). The benefit of this study is that it is the first research in Ukraine conducted at national level onthis type of nesting by Cormorants. Moreover, the study examines the history of emergence of these nesting territories and population dynamics of the Great Cormorant from the time of initial settlement of the breeding sites till the present.


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