Seroprevalence of Seven Reproductive Diseases in Beef and Dairy Cows from Three Provinces in Indonesia

Bovine reproductive diseases are endemic in Indonesia, but comprehensive information about their infectious causes is not available. Therefore, our aim for this study was to detect several infectious agents that cause reproductive diseases in Indonesian beef and dairy cows. A total of 152 cow serum samples collected by Faculty of Veterinary Medicine of Brawijaya University and Veterinary Disease Investigation Centre as a part of the mandatory and regularly surveillance system from three provinces during 2019–2020 were used. The samples were then sent to Indonesian Research Centre for Veterinary Science (IRCVS) for further detection of seven reproductive diseases by enzyme-linked immunosorbent assay (ELISA). Seven reproductive diseases to be tested in parallel are neosporosis, chlamydiosis, brucellosis, Q fever, bovine viral diarrhea (BVD), infectious bovine rhinotracheitis (IBR), and BHV-4 infection. The dominant reproductive diseases in Indonesian cows were BVD (45.69%), chlamydiosis (31.58%), IBR (20.53%), neosporosis (11.84%), and BHV-4 infection (10.53%). The seroprevalence of IBR, BHV-4 infection, neosporosis, and brucellosis varied significantly P < 0.05 between dairy and beef cattle. The most dominant reproductive diseases in aborted cows were chlamydiosis (45%), BVD (41%), and neosporosis (10%). The conclusion drawn from this study is that the dominant reproductive diseases in Indonesian cows are BVD, chlamydiosis, IBR, neosporosis, and BHV-4 infection. Chlamydiosis, BVD, and neosporosis are common among aborted cow. Chlamydiosis, neosporosis, and BHV-4 infection should be included in the national priority list in Indonesia. Control and preventive measures should be focused on high-risk areas and animals like stray cat and dog.

Causes of Death in Stray Cat Colonies of Milan: A Five-Year Report

The presence of cats in urban environments has a long history. In Italy, stray cats are protected by national and regional laws, and programs of neutering and reintroduction to colonies are ongoing. Colony cats have been widely studied from a behavioral perspective, while surveys regarding their causes of death are limited, although they may provide relevant information related to public health and cat welfare. This retrospective study provides pathological descriptions and statistical analyses of the causes of death of 186 cats from 100 colonies in the city of Milan. Inflammatory processes represent the primary cause of death (37.7%) and include common feline infectious diseases such as feline panleukopenia (67.5%), particularly in kittens, and feline infectious peritonitis (32.5%), most common in adult cats. Trauma was found to be a common cause of death of young/adult cats (14%) with a generally good body condition, while severe parasitosis was less represented (2.6%). The death of old cats was statistically associated with organ failure (24.7%), particularly renal failure, and tumors (11.8%). Knowledge of the most common causes of death of colony cats could make an important contribution to the health monitoring of these cats and sanitary control of their habitats and provide information on possible related emerging animal welfare concerns.

Gastrointestinal Parasite Infection on Stray Cat and Pet Cat at Blitar Regency, East Java Province

The purpose of this study was to find out the parasite species and prevalence of gastrointestinal parasite on stray cat and pet cat at Blitar. In an amount of 90 cats feces were collected from two different types of sample, those are stray cat and pet- cat from three sub-districts located at Blitar Regency. Cats feces were analyzed with native, sedimentation and float techniques. The result showed that prevalence of gastrointestinal parasite infection on cat at Blitar was amounted to 71% at stray cat and 40% at pet cat. The parasite that was found in this research are Toxocara cati, Ancylostoma sp. and Isospora sp.

Molecular Characterization of a Natural Mutant of Feline Calicivirus in China

During epidemiological surveillance of Feline calicivirus (FCV) isolates in Shanghai, China, a natural mutant of FCV, designated SH1909, was successfully isolated from a stray cat. The complete genome sequence of SH1909 was determined in this study. Sequence comparison and analysis showed that thirteen unique aa residues substitutions and single-aa insertion of N or Y were observed in SH1909, which indicated that SH1909 was a novel and natural mutant of FCV. Interestingly, phylogenetic analysis based on LC-VP1 showed SH1909 could be clustered into an independent evolutionary branch with some Chinese isolates and was more distantly related to vaccine strains, indicating its potential to escape from vaccine-elicited immunity. According to the predicted B-cell epitopes of LC-VP1, amino acid mutation sites and positive selective sites, peptide C (aa sites: 445–460) and, peptide D (aa sites: 425–440) located in the hypervariable regions of LC-VP1, may result in the decreased immunological protection. Moreover, amino acid sites 439 and 449 may be responsible for the potential immune escape of SH1909. This study provides an important insight into genetic variations of FCV and vaccine development.

Trap-Neuter-Return: A Study of the Practice in Switzerland

Trap-Neuter-Return (TNR) is well-established in many countries and increasingly considered to be the most effective and humane way to manage feral and stray cat populations. Nonetheless, it confronts major challenges everywhere it is practiced. Although Switzerland has an impressive TNR system carried out mainly by private, non-profit organizations and individual citizens, the management of feline overpopulation could be strengthened by improvements on a number of fronts, including legal and governance changes, education and modifications in mentality, and operational adjustments such as data management and analysis. TNR faces a number of common challenges worldwide, and given that it is still relatively young, lessons from one country can be valuable for another. Because little has been written about TNR in Switzerland, this article seeks to fill this gap by providing a preliminary analysis of the case through the prism of existing analytical work done on TNR in other countries.

Genetic Characterization of Feline Parvovirus Isolate Fe–P2 in Korean Cat and Serological Evidence on Its Infection in Wild Leopard Cat and Asian Badger

Feline parvovirus (FPV) is a small, non-enveloped, single-stranded DNA virus that infects cats. We recently isolated a feline parvovirus Fe–P2 strain from a dead stray cat in Iksan, 2017. Its partial genomic sequence (4,643 bases) was obtained, and phylogenetic analysis based on the VP2 nucleotide sequence showed that the FPV Fe-P2 strain was closely related to the FPV isolate Gigucheon in cat, 2017 (MN400978). In addition, we performed a serum neutralization (SN) test with the FPV isolates in various mammalian sera. These were from raccoon dog, water deer, Eurasian otter, Korean hare, leopard cat, and Asian badger, which were kindly provided by Chungnam Wild Animal Rescue Center. Notably, serological evidence of its infection was found in Asian badger, Meles leucurus (2/2) and leopard cat, Prionailurus bengalensis (5/8) through SN tests, whereas there was no evidence in raccoon dog, water deer, Eurasian otter, and Korean hare based on the collected sera in this study. These findings might provide partial evidence for the possible circulation of FPV or its related viruses among wild leopard cat and Asian badger in Korea. There should be additional study to confirm this through direct detection of FPVs in the related animal samples.

Toxoplasma gondii prevalence and risk factors in owned domestic cats from Nakhon Pathom Province, Thailand

Domestic cats are a potential source of Toxoplasma gondii infection for humans. This study was conducted to determine the seroprevalence and risk factors for T. gondii infection in domestic cats. Cat sera (n = 182) were tested for T. gondii IgG antibodies using the latex agglutination test (LAT) and the GRA7 of T. gondii (TgGRA7)-indirect enzyme-linked immunosorbent assay (iELISA). Univariable and multivariable logistic regression analyses were used to identify the factors associated with T. gondii infection. The overall prevalence rates were 18.1% (33/182) according to LAT, 19.2% (35/182) according to the iELISA and 17.0% (31/182) according to LAT and iELISA. Univariable analyses identified, outdoor access (P = 0.006), being a former stray cat (P = 0.001) and successful hunting behaviors (P = 0.04) as risk factors for T. gondii infection. Outdoor access (OR 2.63, 95% confidence interval (CI) 1.03–6.72) and is a former stray cat (OR 3.69, 95% CI 1.52–8.96) remained significant risk factors in multivariable analyses. This study indicated a relatively high seroprevalence of T. gondii among domestic cats. Cat owners can reduce the risk for T. gondii infection by not allowing their cats to roam free. Furthermore, education about the transmission of the parasite should be provided to prevent infection to the owners.

Manual Strangulation of a stray cat

Veterinary forensic pathology plays a crucial role in animal death investigations. Recent years have seen increased attention given to this field in Taiwan. Here we report a grave crime that aroused public indignation in Taiwan, when in December 2015, a missing free-roaming cat was allegedly strangled by a suspect, according to witnesses. Surveillance video footage of the cruel incident surfaced that showed the cat, without visible signs of struggle, strangled by both hands of the suspect and kicked in the abdomen’s left side. The body was stored in a travel bag when found in the suspect’s motorcycle trunk three days after the cat had been missing. The radiographs revealed a suspected luxation of cervical vertebrae 6 and 7 (C6–C7). At forensic necropsy and histopathology, neck compression with subcutaneous hemorrhage at the left submandibular area, bruising of the neck, tears in the wall of the left external jugular vein, and pulmonary lesions were identified, all consistent with asphyxiation. Hemorrhages of the epidural space of the C7 vertebra, the spinal nerve at the level of C7, and surrounding soft tissues were noted. The pathological findings were in line with the suspect’s confession, the witnesses’ statements, and the video footage. The process and outcome of this case reflected society’s growing awareness of animal welfare and the increased attention that the authorities are giving crimes against animals in Taiwan, and the link between pathologic findings and the crime.

Complete Genome Sequence of a Natural Mutant of Feline Calicivirus Isolated From a Stray Cat

During the period of the epidemiological surveillance of Feline calicivirus (FCV) isolates, a natural mutant of FCV, designated SH1909, was successfully isolated from a stray cat in Shanghai, China. The complete genome sequence of SH1909 was also determined in this study. Sequence comparison and analysis show that thirteen unique aa residues substitutions and single-aa insertion of N or Y were observed in SH1909 when compared with other FCV isolates. Phylogenetic analysis showed that SH1909 was assigned into a major evolutionary branch and distantly related to vaccine strains. These results indicated that SH1909 was a natural mutant of FCV circulating in China, which has the potential to escape from immunity of FCV vaccine.