Colon tissue concentrations of copper, iron, selenium, and zinc in colorectal carcinoma patients

2006 ◽  
Vol 60 (4) ◽  
Author(s):  
P. Hornik ◽  
D. Milde ◽  
Z. Trenz ◽  
K. Vysloužil ◽  
V. Stužka
Keyword(s):  
Trace Elements ◽  
Reference Material ◽  
Absorption Spectrometry ◽  
Colon Tissue ◽  
Tissue Samples ◽  
Alkaline Digestion ◽  
Microwave Assisted ◽  
Digestion Technique ◽  
Efficient Alternative ◽  
Copper Zinc

AbstractConcentration of four trace elements (copper, zinc, iron, and selenium) was determined in colon tissue from twenty-three patients with colorectal cancer. The tissue samples were dried and dissolved using microwave-assisted digestion technique. The content of trace elements was determined using atomic absorption spectrometry. No significant differences between Zn levels in normal and cancerous tissues were observed. However, the levels of Cu, Fe, and Se were significantly higher in cancerous tissues than in the control samples.The results obtained sing microwave-assisted technique were compared to those obtained via alkaline digestion with tetramethylammonium hydroxide. Both techniques were used for the digestion of certified reference material of pork liver. Very good agreement with the certified values of Cu, Fe, and Zn in the reference material was achieved using alkaline digestion procedure. This technique seems to be an efficient alternative to decomposition of biological samples.

Micro-quantity tissue digestion for metal measurements by use of a microwave acid-digestion bomb.

1989 ◽  
Vol 35 (3) ◽  
pp. 488-490 ◽  
Author(s):  
J R Nicholson ◽  
M G Savory ◽  
J Savory ◽  
M R Wills
Keyword(s):  
Nitric Acid ◽  
Reference Material ◽  
Microwave Digestion ◽  
Absorption Spectrometry ◽  
Bovine Liver ◽  
Dry Weight ◽  
Acid Digestion ◽  
Tissue Samples ◽  
Dry Ashing ◽  
Tissue Digestion

Abstract We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamination or loss from volatilization. Small quantities of tissue (5-100 mg dry weight) are digested in high-purity nitric acid, yielding concentrations of analyte that can be measured directly without further sample manipulation. We analyzed National Institute of Standards and Technology bovine liver Standard Reference Material to verify the accuracy of the technique. We assessed the applicability of the technique to analysis for aluminum in bone by comparison with a dry ashing procedure.

scholarly journals Validation of Total Mercury in Marine Sediment and Biological Samples, Using Cold Vapour Atomic Absorption Spectrometry

2018 ◽  
Vol 1 (3) ◽  
pp. 31 ◽  
Author(s):  
Ahmed Elezz ◽  
Hassan Mustafa Hassan ◽  
Hamood Abdulla Alsaadi ◽  
Ahmed Easa ◽  
Saeed Al-Meer ◽  
...  
Keyword(s):  
Trace Elements ◽  
Reference Material ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Marine Sediment ◽  
Environmental Samples ◽  
Total Mercury ◽  
Absorption Spectrometry ◽  
Freeze Dried ◽  
Cold Vapour

A method for the measurement of total mercury (T-Hg) in environmental samples using cold vapour atomic absorption spectrometry (CV AAS) has been validated yielding a dynamic range (0.04–10.00 μg/kg) and high certified reference material (CRM) recovery (>90%). The validation was carried out according to International Union of Pure and Applied Chemistry (IUPAC) validation and Eurachem Guides. A freeze-dried and homogenised sample was weighed and then digested using Suprapur acids (HNO3, H2SO4, and HF) with potassium dichromate solution in a hot block digestion system. A calibration curve was constructed (R2 > 0.999). Two CRMs (Marine Sediment Reference Material (PACS-3) and Trace Elements in Muscle Tissue (Trace Elements and Methylmercury in Mussel Tissue (NIST2976)) were utilised for quality assurance and control. The limit of quantification (LOQ) calculated as 0.04 µg/kg, and uncertainty (U) calculated as 2%. The obtained results showed the suitability of this method for direct mercury measurement in environmental samples. Additionally, the proficiency of this method was recognised by accreditation under the standard of International Organization for Standardization (ISO/IEC 17025:2017) for competence of testing and calibration laboratories.

A New Coprecipitation Methodology with Lutetium Hydroxide for Preconcentration of Heavy Metal Ions in Herbal Plant Samples

2014 ◽  
Vol 97 (4) ◽  
pp. 1189-1194 ◽  
Author(s):  
Mustafa Soylak ◽  
Ipek Murat
Keyword(s):  
Heavy Metal ◽  
Trace Elements ◽  
Reference Material ◽  
Heavy Metal Ions ◽  
Absorption Spectrometry ◽  
Aqueous Sample ◽  
Present Procedure ◽  
Plant Samples ◽  
Herbal Plant ◽  
Spinach Leaves

Abstract A new coprecipitation methodology that used lutetium hydroxide as a precipitant for Cu(II), Pb(II), Mn(II), Co(II), Cd(II), Fe(III), and Ni(II) ions in herbal plant and water samples for analysis by atomic absorption spectrometry has been investigated. The parameters such as pH, amount of lutetium, and volume of aqueous sample were optimized for the recovery of these seven metals. The effects of concomitant ions on the separation-preconcentration of analytes were also checked. The validation of the procedure was checked with addition recovery tests and analysis of Standard Reference Material 1570a-Trace Elements in Spinach Leaves and TMDA-70 fortified lake water Certified Reference Material. The LODs for analyte ions were in the range of 1.7–7.2 μg/L. The application of the present procedure was successfully performed for the analysis of analyte contents of herbal plant samples from Turkey.

scholarly journals Evaluation of zinc and copper levels in vaginal tissues and whole blood: correlation with age

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Anett Csikós ◽  
Bence Kozma ◽  
Edina Baranyai ◽  
Ida Miklós ◽  
Kindra Larson ◽  
...  
Keyword(s):  
Trace Elements ◽  
Vaginal Delivery ◽  
Whole Blood ◽  
Menopausal Status ◽  
Emission Spectrometry ◽  
Blood Levels ◽  
Vaginal Tissue ◽  
Tissue Samples ◽  
Copper Zinc ◽  
Zinc Levels

Abstract Background Zinc and copper are essential trace elements and play a crucial role in the homeostasis of connective tissues. In this study, we aimed to define zinc and copper levels in the vaginal tissue and establish whether a correlation exists between the zinc and copper levels either or both in whole blood or vaginal tissue samples and whether the finding correlates with the age of the patient or at least with her menopausal status. Methods We collected whole blood and vaginal tissue samples from 32 women and measured their zinc and copper levels by inductively coupled plasma optical emission spectrometry. We have performed Student's t test to evaluate the differences in the mean levels of trace elements and multiple regression to evaluate the association between vaginal tissue zinc/copper levels and age, menopausal status, number of vaginal deliveries, and zinc/copper blood levels. Results Zinc levels were significantly higher in both the vaginal tissues and whole blood samples than copper levels (p < 0.01). In the vaginal tissue samples, a strong positive correlation could be detected between zinc and copper levels (r = 0.82, p < 0.01). In the vaginal tissue, a negative correlation was found for zinc and copper levels with the age of women (r = − 0.27, p = 0.04 and r = − 0.56, p < 0.01). Multiple linear regression model (age, menopausal status, vaginal delivery and copper/zinc blood levels) showed that only age remained a significant predictor for zinc and copper vaginal tissues levels (p = 0.03, 95% CI − 2.28 to − 0.06; p = 0.004, 95% CI − 1.76 to − 0.34). Conclusions Zinc and copper levels in the vaginal tissue decline with age. Out of the examined variables (age, menopausal status, vaginal delivery, and copper/zinc levels), only age is a significant predictor of vaginal zinc/copper levels.

Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Bhagavathami Meenaksh ◽  
Chitraa R. Chandran ◽  
Aravindhan Thathchari Ranganathan ◽  
Kavindapadi Venkateswaran Rama ◽  
Valarmathy Srinivasan
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Gingival Crevicular Fluid ◽  
Serum Zinc ◽  
Cross Sectional Study ◽  
Serum Copper ◽  
Cross Sectional ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Copper Zinc

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

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