Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

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The Effect ofHelicobacter pyloriEradication on the Levels of Essential Trace Elements

BioMed Research International ◽

10.1155/2014/513725 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Meng-Chieh Wu ◽

Chun-Yi Huang ◽

Fu-Chen Kuo ◽

Wen-Hung Hsu ◽

Sophie S. W. Wang ◽

...

Keyword(s):

Trace Elements ◽

Inductively Coupled Plasma ◽

Eradication Therapy ◽

Serum Zinc ◽

Selenium Concentration ◽

Serum Copper ◽

Serum Selenium ◽

Significant Difference ◽

Copper Zinc ◽

H Pylori

Objective. This study was designed to compare the effect ofHelicobacter pylori(H. pylori) infection treatment on serum zinc, copper, and selenium levels.Patients and Methods. We measured the serum zinc, copper, and selenium levels inH. pylori-positive andH. pylori-negative patients. We also evaluated the serum levels of these trace elements afterH. pylorieradication. These serum copper, zinc, and selenium levels were determined by inductively coupled plasma mass spectrometry.Results. Sixty-threeH. pylori-positive patients and thirtyH. pylori-negative patients were studied. Serum copper, zinc, and selenium levels had no significant difference betweenH. pylori-positive andH. pylori-negative groups. There were 49 patients with successfulH. pylorieradication. The serum selenium levels were lower after successfulH. pylorieradication, but not significantly (P=0.06). There were 14 patients with failedH. pylorieradication. In this failed group, the serum selenium level afterH. pylorieradication therapy was significantly lower than that beforeH. pylorieradication therapy (P<0.05). The serum zinc and copper levels had no significant difference between before and afterH. pylorieradication therapies.Conclusion.H pylorieradication regimen appears to influence the serum selenium concentration (IRB number: KMUH-IRB-20120327).

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Trace elements in patients with aortic valve sclerosis

Therapeutic Advances in Cardiovascular Disease ◽

10.1177/1753944720985985 ◽

2021 ◽

Vol 15 ◽

pp. 175394472098598

Author(s):

Hataw Al-Taesh ◽

Abuzer Çelekli ◽

Murat Sucu ◽

Seyithan Taysi

Keyword(s):

Trace Elements ◽

Aortic Valve ◽

Inductively Coupled Plasma ◽

Healthy Subjects ◽

Serum Zinc ◽

Serum Levels ◽

Control Group ◽

Aortic Valve Sclerosis ◽

Inductively Coupled ◽

Significant Difference

Background: Aortic valve sclerosis (AVSc) is defined as the thickening and calcification of aortic valve cusps, in the absence of obstruction of ventricular outflow. AVSc is linked with a clear imbalance in some trace elements. Aims: The objective of this study was to investigate the relationship between AVSc and serum levels of iron (Fe), zinc (Zn), selenium (Se), and copper (Cu). Additionally, this research aimed to explore the clinical significance of human serum zinc, selenium, copper, and iron concentrations as a potential new biomarker for AVSc patients and to clarify the pathophysiological role in individuals at risk of developing AVSc. Patients and methods: The study included 40 subjects with AVSc (25% male and 75% female) who were compared with a healthy control group with the same gender ratio. AVSc was based on comprehensive echocardiographic assessments. Blood samples were taken and Zn and Cu concentrations were determined through the use of atomic absorption spectroscopy. Se was measured using an inductively coupled plasma mass spectrometry device and Fe was measured using a Beckman Coulter instrument. Results: There was a significant difference in the prevalence of diabetes, blood pressure levels, and body mass index between the patients and the healthy subjects ( p < 0.05). The differences between the serum Fe, Se, and Cu levels of the AVSc patients and the healthy subjects ( p > 0.05) were recorded. The serum Zn of AVSc patients when compared was significantly lower compared with that of the control group ( p < 0.01). Conclusion: Patients with AVSc had an imbalance in some of the trace elements in their blood. The patient group’s valves had higher serum Cu levels and lower serum Se, Zn, and Fe concentrations compared with the healthy group’s valves. In the valve patients as compared, AVSc had a high prevalence of obesity, hypertension, and diabetes.

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Serum Zinc, Copper and Calcium Levels In Female Rheumatoid Arthritic Patients

Journal of Bangladesh Society of Physiologist ◽

10.3329/jbsp.v7i1.11155 ◽

2012 ◽

Vol 7 (1) ◽

pp. 18-22

Author(s):

Arifa Alam Jelly ◽

Shelina Begum ◽

Sultana Ferdousi

Keyword(s):

Rheumatoid Arthritis ◽

Colorimetric Method ◽

Serum Zinc ◽

Cross Sectional Study ◽

Serum Copper ◽

Copper Level ◽

Cross Sectional ◽

Healthy Female ◽

Copper Zinc ◽

Medical University

Background: Rheumatoid Arthritis is a chronic multi-system autoimmune inflammatory disease, characterized by inflammatory synovitis involving symmetrical peripheral joints. Serum copper, zinc and calcium levels are altered in Rheumatoid Arthritis.Objective: To observe serum zinc, copper and calcium levels in patients with Rheumatoid Arthritis (RA).Methods: This cross sectional study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka from January to December 2010. For this, 60 female patients with Rheumatoid arthritis aged 30-50 years were included in the study group. The patients were selected from outpatient Department of Rheumatology wing of medicine in Bangabandhu Sheik Mujib Medical University (BSMMU). For comparison age and BMI matched 30 apparently healthy female were also studied. Serum calcium level was measured by Colorimetric method. Serum zinc and copper was measured by Spectrophotometric method. For statistical analysis, independent sample t test was performed by using SPSS for windows version-12.Results: In this study, serum zinc and calcium levels were significantly lower (p<0.001) and serum copper level was significantly higher (p<0.001) in the RA patients than those of control.Conclusion: This study may conclude that hypozincemia and hypocalcemia and hypercupremia occur in RA. Therefore early detection of these micronutrient abnormalities may be helpful for the physicians to prevent various complications in RA patients. DOI: http://dx.doi.org/10.3329/jbsp.v7i1.11155 J Bangladesh Soc Physiol. 2012, June; 7(1): 18-22

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Evaluation of Trace Elements in the Nails and Hair of Farmers Exposed to Pesticides and Fertilizers

Journal of Agricultural Science ◽

10.5539/jas.v9n13p79 ◽

2017 ◽

Vol 9 (13) ◽

pp. 79

Author(s):

Zariyantey Abdul Hamid ◽

Ismarulyusda Ishak ◽

Syarif Husin Lubis ◽

Nihayah Mohammad ◽

Hidayatulfathi Othman ◽

...

Keyword(s):

Trace Elements ◽

Inductively Coupled Plasma ◽

Pesticide Exposure ◽

Demographic Data ◽

Cross Sectional Study ◽

Zinc Level ◽

Dietary Habit ◽

Cross Sectional ◽

Inductively Coupled ◽

Hair Samples

Exposure to pesticides and fertilizers lowers the level of trace elements in the human body for several reasons. This study aimed to investigate the effect of pesticide exposure to the levels of trace elements of farmers in Bachok and Tumpat, Kelantan, Malaysia. This cross sectional study involved 89 farmers. Demographic data and information on the duration of the exposure to the pesticides and fertilizers, as well as personal protective equipment (PPE) practice habits, were determined through questionnaire. The levels of selenium, manganese, zinc, copper and chromium samples of fingernails, toenails and hair were determined through the use of inductively coupled plasma-mass spectrometry (ICP-MS). The levels of the trace elements were not influenced by gender, age and the period of exposure. Only the manganese levels found in the hair samples (r = 0.250) show a significant positive correlation (p < 0.05) with the working period. PPE practice habit also have significant correlation (p < 0.05) with manganese level in fingernails (r = 0.530) and toenails (r = -0.353), zinc level in hair (r = -0.439) and chromium level in fingernails (r = -0.306). Exposure towards pesticide and fertilizer decreased the trace element level in nails and hair of farmers. Additionally, the level of trace elements can be influenced by health status, working period, dietary habit and PPE practices.

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Prognostic factors associating with depression score in male patients; Inflammation, oxidative stress and serum copper and zinc: A cross-sectional study

10.21203/rs.3.rs-22430/v1 ◽

2020 ◽

Author(s):

Majid Ghayour-Mobarhan ◽

Susan Darroudi ◽

Batool Tayefi ◽

Narjes Khalili ◽

Haniyeh Darroudi ◽

...

Keyword(s):

Serum Zinc ◽

Cross Sectional Study ◽

Serum Copper ◽

Depression Score ◽

Cross Sectional ◽

Copper Status ◽

Two Factors ◽

Male Patients ◽

Hs Crp ◽

Normal Males

Abstract BackgroundThe prevalence of depression increased about 18% during a 10-year period between 2005 and 2015. The aim of the present study was to investigate the association between serum zinc and copper, PAB, SOD and hsCRP in men with depression.MethodsIn this study, 3768 men, were recruited as part of MASHAD study. Demographic, anthropometric and metabolic data measured and recorded for all subjects. The Beck Depression Inventory (BDI) was used for assessing depression in all participants. Serum zinc and copper concentration, hs-CRP, PAB (Pro-oxidant- Antioxidant Balance), and SOD (Superoxide dismutase) were measured in all subjects.Results:The mean level of copper in males with depression is significantly lower than normal males (p < 0.05); despite the lower level of zinc in depressed subjects, this was not significant (p > 0.05). The median of hs-CRP and mean of PAB were significantly higher in depressed subjects (p < 0.001). Logistic regression analysis was performed to determine the odds ratio (OR) for an association of serum zinc, copper, hs-CRP, SOD and PAB with depression in males. The subjects with a serum copper < 80 µg/dl (Q1) had a 1.337 (1.13–1.582) higher chance of depression and the chance of depression increased 1.021(1.013–1.029) with elevating 1 mg/l of serum hs-CRP. 1.021(1.013–1.029). These data remained the same after adjustment for each other’s. Furthermore, our results strongly suggested that hs-CRP and copper were the independent risk factors for depression in male patients.Conclusion:Inflammation and copper status, not zinc concentration, are two factors that may play a role in depression in male patients.

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Copper and Copper/Zinc Ratio in a Series of Cystic Fibrosis Patients

Nutrients ◽

10.3390/nu12113344 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3344

Author(s):

Marlene Fabiola Escobedo-Monge ◽

Enrique Barrado ◽

Carmen Alonso Vicente ◽

María Antonieta Escobedo-Monge ◽

María Carmen Torres-Hinojal ◽

...

Keyword(s):

Cystic Fibrosis ◽

Zinc Deficiency ◽

Linear Regression Analysis ◽

Bone Conduction ◽

Serum Zinc ◽

Abdominal Ultrasound ◽

Serum Copper ◽

Cross Sectional ◽

Inflammatory Status ◽

Copper Zinc

Cystic fibrosis (CF) patients require a stable and sufficient supply of micronutrients. Since copper is an essential micronutrient for human development, a cross-sectional study was carried out to investigate the serum copper levels, serum copper/zinc (Cu/Zn) ratios, and their relationship with nutritional indicators in a group of CF patients. Anthropometric, biochemical, and dietary measurements, an abdominal ultrasound, and respiratory and pancreatic tests were conducted. Seventeen CF patients were studied (10 females, 59%), 76.5% of whom were ∆F580. Their mean serum copper (113 ± 23 μg/dL) was normal, and there was only one teenager with hypocupremia (6%) and two children with hypercupremia (18%). A significant association between serum copper and zinc levels was discovered. The Cu/Zn ratio was higher than 1.00 for 94% of patients, which is an indicator of an inflammation status. There was no significant correlation between the serum copper concentrations and respiratory and pancreatic function, respiratory colonization, and the results of the abdominal ultrasound. Linear regression analysis showed that serum copper had a positive association with both the Z-score body mass index (BMI) and mean bone conduction speed (BCS). Therefore, since 94% of CF patients had a Cu/Zn ratio > 1.00, this factor must alert us to consider the risk of zinc deficiency and high inflammatory response. The measurement of serum zinc alone does not show one’s zinc status. However, the Cu/Zn ratio may be an indicator of zinc deficiency and the inflammatory status of CF patients.

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A Comparative Study Between Conventional ICP-OES and the Innovative PLS Model-Assisted ICP-OES for the Assay of Trace Elements

Journal of AOAC International ◽

10.1093/jaoacint/qsaa090 ◽

2020 ◽

Author(s):

Adel M Michael ◽

Ahmed A Mohamed ◽

Yousef A Abdelaziz ◽

Nesma M Fahmy

Keyword(s):

Trace Elements ◽

Elemental Analysis ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Official Method ◽

Icp Oes ◽

Inductively Coupled ◽

Flame Emission ◽

Significant Difference ◽

Student’S T

Abstract Background Inductively coupled plasma is widely used for elemental analysis with the advantage of being eco-friendly since the discharge is free of contaminants. Objective A rapid, novel method was developed for the quantitation of trace elements using inductively coupled plasma with optical emission spectrometry.This method has the advantage of simultaneous calibration compared to the conventional method. Method The assay was carried out for iron, copper, zinc, and molybdenum using the linear regression model partial least-squares. Results The method was optimized and validated as per the International Conference on Harmonization guidelines, showing highly accurate and precise results. The linearity range was 0.25–4 ppm for all trace elements under investigation. The method was applied for the assay of the cited elements in non-chelated and amino acid chelated multi-mineral preparations in the Egyptian market with acceptable mean percent recovery. Conclusions In comparison with the official method by flame emission, statistical analysis showed no significant difference with Student’s t-test and F-values. Highlights Inductively coupled plasma is superior as all of the elements can be measured simultaneously. The method was found to have a high degree of specificity and can be easily applied in routine elemental analysis in laboratories.

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Salivary Levels of IL-6 and IL-17 Could Be an Indicator of Disease Severity in Patients with Calculus Associated Chronic Periodontitis

BioMed Research International ◽

10.1155/2018/8531961 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 15

Author(s):

Husniah Batool ◽

Ahmed Nadeem ◽

Muhammad Kashif ◽

Faheem Shahzad ◽

Romeeza Tahir ◽

...

Keyword(s):

Chronic Periodontitis ◽

Alveolar Bone ◽

Gingival Crevicular Fluid ◽

Severe Disease ◽

Cross Sectional Study ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Cross Sectional ◽

Significant Difference ◽

Crevicular Fluid

Background/Purpose. Chronic periodontitis is an inflammatory disease of gums that causes loss of supporting structures of teeth, that is, gingiva, periodontal ligament, cementum, and alveolar bone. Levels of various cytokines in the serum, gingival tissues, and gingival crevicular fluid in patients with chronic periodontitis have been studied, but limited data are available on the level of cytokines in saliva. Therefore, a study was designed to determine levels of salivary IL-6 and IL-17 in patients with calculus associated chronic periodontitis. Materials and Methods. It was a comparative, cross-sectional study that is comprised of 41 healthy controls and 41 calculus associated chronic periodontitis patients (CP patients). According to the degree of attachment loss, CP patients were subcategorized as mild (CAL 1-2 mm), moderate (CAL 3-4 mm), and severe (CAL > 5 mm) forms of periodontitis. Salivary levels of IL-6 and IL-17 were determined using enzyme-linked immunosorbent assay (ELISA) technique. Data was analyzed using SPSS 20.0. Results. Between healthy controls and CP patients (moderate and severe disease), a statistically significant difference was observed in the concentrations of IL-6 and IL-17. In CP patients, the highest mean ± SD of salivary IL-6 and IL-17 was observed in severe CP, followed by moderate and mild CP. Regarding level of IL-6, a statistically significant difference was observed between mild and severe disease and between moderate and severe subcategories of CP patients. Similarly, statistically significant difference was observed in the level of IL-17 between mild and moderate, mild and severe disease, and moderate and severe disease. Conclusion. The levels of salivary IL-6 and IL-17 were increased significantly in calculus associated CP patients as compared to healthy controls and these levels increased with the progression of CP. Clinical Significance. Salivary levels of IL-6 and IL-17 may help in the subcategorization of CP.

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TO INVESTIGATE THE EFFECT OF THE PASTEURISATION PROCESS ON TRACE ELEMENTS IN DONOR BREAST MILK

Archives of Disease in Childhood ◽

10.1136/archdischild-2016-311535.27 ◽

2016 ◽

Vol 101 (9) ◽

pp. e2.20-e2 ◽

Cited By ~ 2

Author(s):

Nor Mohd Taufek ◽

David Cartwright ◽

Amitha Hewavitharana ◽

Pieter Koorts ◽

Helen McConachy ◽

...

Keyword(s):

Trace Elements ◽

Breast Milk ◽

Inductively Coupled Plasma ◽

Rank Correlation ◽

Formula Milk ◽

Donor Milk ◽

Inductively Coupled ◽

Spearman’S Rank Correlation ◽

Milk Bank ◽

Significant Difference

AimTo investigate the effect of the pasteurisation process on trace elements in donor breast milk.MethodPremature infants often receive donor breast milk when the mother is unable to produce sufficient breast milk. It is widely accepted that donor milk has considerable advantages over formula milk.1 The Royal Brisbane and Women's Hospital (RBWH) has a milk bank that receives milk donated by women which undergoes a pasteurisation process.2 This study investigated the effect of pasteurisation on a range of trace elements in donor milk.A total of 14 participants who donated to the milk bank were recruited in this study. A 2 ml sample was collected pre- and post- pasteurisation, and frozen at −80 °C. Post-natal age of the milk was documented. Inductively-coupled plasma mass-spectrometry was used to analyse the following trace elements – zinc (Zn), copper (Cu), selenium (Se), manganese (Mn), iodine (I), iron (Fe), molybdenum (Mo) and bromine (Br). The study received ethical approval from RBWH and The University of Queensland Ethics Committee.ResultsNo significant difference was found between the levels of any of the trace elements tested pre- and post-pasteurisation. The following p-values were calculated – Zn (0.82), Cu (0.80), Se (0.97), Mn (0.63), I (0.99), Fe (0.05), Mo (0.41), Br (0.59). The following ranges in mcg/L of trace elements were calculated – Zn (365.4–5460.0), Cu (157.6–820.5), Se (10.6–23.7), Mn (0.55–3.24), I (66.4–215.3), Fe (101.5–473.1), Mo (0.20–5.45), Br (704.9–3379.0). Spearman's rank correlation analysis showed significant correlations between post-natal age of milk and trace elements – Zn (ρ=−0.578), Se (ρ=−0.627). Fe (ρ=−0.704), and Mo (ρ=−0.534). No significant correlation was found for Cu, Mn, I, and Br.ConclusionThis study found that the pasteurisation process had minimal effect on trace element levels in donor breast milk. However, it was noted that there was a correlation between post-natal age of donor milk and Zn, Se, Fe and Mo. Further work is needed to establish factors that may influence levels of trace elements in donor milk such as post-natal age.

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Homocysteine, trace elements and oxidant/antioxidant status in mild cognitively impaired elderly persons: a cross-sectional study

Romanian Journal Of Internal Medicine ◽

10.1515/rjim-2015-0043 ◽

2015 ◽

Vol 53 (4) ◽

pp. 336-342 ◽

Cited By ~ 1

Author(s):

Hajar Negahdar ◽

Seyed Reza Hosseini ◽

Hadi Parsian ◽

Farzan Kheirkhah ◽

Abbas Mosapour ◽

...

Keyword(s):

Trace Elements ◽

Cognitive Impairment ◽

Antioxidant Status ◽

Clinical Signs ◽

Cross Sectional Study ◽

Control Group ◽

Elderly Persons ◽

Effective Parameters ◽

Cross Sectional ◽

Significant Difference

Abstract Introduction. Mild cognitive impairment (MCI) is the prodromal stage of Alzheimer’s disease (AD), so identification of the related risk factors can be helpful. There are controversial data regarding the serum oxidant/antioxidant status, trace elements and homocysteine (Hcy) as effective parameters in this disease, therefore the status of these factors was determined in this study. Methods. One hundred-twenty elderly persons with cognitive impairment and 120 elderly healthy persons who were differentiated using Mini-mental state examination (MMSE) participated in this study. The patients were divided into mild and moderate-to-severe cognitive impairment group. Serum antioxidant/oxidant, copper (Cu), manganese (Mn), zinc (Zn) and Hcy concentrations were measured using routine methods. Results. Oxidant and antioxidant levels increased and decreased based on the severity of the disease and were higher and lower in patients than in control group, respectively (p<0.001). With adjusting for age, gender and education, significant difference in Hcy levels was not observed. There was no significant difference in trace elements levels among groups. Conclusions. Results confirmed the association between oxidative damage with increasing the severity of cognitive impairment. These factors may be involved in the etiology of cognitive impairment and AD. Identification of such biomarkers is important to select appropriate treatment goals before the onset of irreversible clinical signs.

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