Interaction of Moringa oleifera seed lectin with humic acid

AbstractThe aim of this work was to characterise the affinity of protein preparations from Moringa oleifera seeds, specifically extract (seeds homogenised with 0.15 M NaCl), fraction (extract precipitated with 390 mg mL−1 of ammonium sulphate) and cMoL (coagulant M. oleifera lectin) to bind humic acids using a haemagglutinating activity assay with rabbit erythrocytes and a radial diffusion assay in agarose gel. Specific haemagglutinating activity (SHA) decreased by 94 % for the extract and cMoL and by 50 % for the fraction in the presence of humic acid. Precipitation bands were observed in the diffusion gel. Both results suggested humic acid-cMoL binding. Carbohydrates, potassium, and calcium ions and pH affected the SHA of cMoL. As an example of application, cMoL was immobilised on a column packed with sepharose receiving 20 mg mL−1 of carbon humic acid solution, 30 mg of humic acid per gram of support was removed. This result suggested that protein preparations might be used in water treatment to remove humic acids.

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A simple purification and activity assay of the coagulant protein from Moringa oleifera seed

Water Research ◽

10.1016/j.watres.2005.04.012 ◽

2005 ◽

Vol 39 (11) ◽

pp. 2338-2344 ◽

Cited By ~ 200

Author(s):

Kebreab A. Ghebremichael ◽

K.R. Gunaratna ◽

Hongbin Henriksson ◽

Harry Brumer ◽

Gunnel Dalhammar

Keyword(s):

Moringa Oleifera ◽

Activity Assay ◽

Moringa Oleifera Seed

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Comparison of the performance of Moringa oleifera seed protein and polyaluminum chloride in removal of humic acid from water

10.5004/dwt.2017.21350 ◽

2017 ◽

Vol 90 ◽

pp. 214-219 ◽

Cited By ~ 2

Author(s):

Jingxi Tie ◽

Zhihong Zheng ◽

Xiaomei Zheng ◽

Yaru Hao

Keyword(s):

Humic Acid ◽

Moringa Oleifera ◽

Seed Protein ◽

Polyaluminum Chloride ◽

Moringa Oleifera Seed

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Changes in the Humic Acid–Metal Complexation Characteristics Depending on Humification Degree / Humīnskābju – Metālu Kompleksveidošanās Rakstura Izmaiņas Atkarībā No Humifikācijas Pakāpes

Latvian Journal of Chemistry ◽

10.2478/v10161-012-0012-0 ◽

2012 ◽

Vol 51 (3) ◽

pp. 228-237

Author(s):

D. Dudare ◽

M. Klavins

Keyword(s):

Humic Acid ◽

Stability Constants ◽

Humic Acids ◽

Complex Stability ◽

Peat Layer ◽

Raised Bog ◽

Complexing Capacity ◽

Humification Degree ◽

Complexation Process ◽

Complex Stability Constants

The aim of this study is to determine the Cu(II) complexing capacity and stability constants of Cu(II) complexes of humic acids isolated from two well-characterized raised bog peat profiles in respect to the basic properties and humification characteristics of the studied peats and their humic acids. The complex stability constants significantly change within the studied bog profiles and are well correlated with the age and decomposition degree of the peat layer from which the humic acids have been isolated. Among factors that influence this complexation process, molecular mass and ability to form micellar structures (supramolecules) of humic substances are of key importance.

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EFFECT OF IRRIGATION AND GRASSING TO CHANGE SOME OF THE BIOLOGICAL PROPERTIES OF THE MINING OF BROWN CALCAREOUS SOILS

Agricultural Technologies ◽

10.35599/agritech/01.01.04 ◽

2019 ◽

Vol 1 (1) ◽

pp. 29-32

Author(s):

Ruzimurod B. Boimurodov ◽

Zebinisso Q. Bobokhonova

Keyword(s):

Folic Acid ◽

Humic Acid ◽

Humic Acids ◽

Rapid Growth ◽

Growth And Development ◽

Sharp Increase ◽

Humus Content ◽

Calcareous Soils ◽

Biological Properties ◽

Humus Accumulation

In this article is showing, that the irrigation mountain brown carbonate soils prone methods of irrigation and grassing comes the rapid growth and development of natural vegetation, which leads to intensive humus accumulation. Humus content in the upper layer is increased by 0.98% and a significantly smaller severely eroded. Increasing the amount of humus promotes accumulation mainly humic acids, that conducts to expansion of relations the content of humic acid: The content of folic acid. When grassing of soil traced sharp increase in the number associated with the related and R2 O3 humic acid.

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Kinetic Adsorption of Humic Acids Mixture Obtained from Microalgae on Exfoliated Graphite Nanoplatelets

Revista de Chimie ◽

10.37358/rc.18.1.6072 ◽

2018 ◽

Vol 69 (1) ◽

pp. 191-195

Author(s):

Elena Radu ◽

Elena Emilia Oprescu ◽

Cristina Emanuela Enascuta ◽

Catalina Calin ◽

Rusandica Stoica ◽

...

Keyword(s):

Humic Acid ◽

Humic Acids ◽

Alkali Treatment ◽

Exfoliated Graphite ◽

Acid Mixture ◽

Acidic Ph ◽

Graphite Nanoplatelets ◽

Mixture Concentration ◽

Ft Ir ◽

Exfoliated Graphite Nanoplatelets

The dehydration of polysaccharides fraction in the presence of acid catalysts, is a chemical process in which results as secondary product humic matter. In our work, the humic acid mixture was for the first time based on our knowledge extracted from defatted microalgae biomass rich in polysaccharides by standard alkali treatment, followed by precipitation at acidic pH. The dried humic acid mixture has been characterized using infrared spectroscopic measurements (FT-IR). Exfoliated graphite nanoplatelets (xGnP) were used as new adsorbents for this type of humic acids mixture, their adsorption being investigated. The effect of several parameters such as: contact time, concentration of humic acid mixture, concentration of xGnP, temperature and pH of the solutions were studied. The process of adsorption took place with good results, in the following conditions: at a concentration of humic acid mixture of 18.6 mg L-1, an xGnP amount of 0.01 mg in 25 mL of solution, at a temperature of 25 �� and at acidic pH values, in aqueous solution.

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Evaluation of Moringa oleifera Seed Lectin as a Metal Remover in Aqueous Solutions

Protein and Peptide Letters ◽

10.2174/0929866523666160517123558 ◽

2016 ◽

Vol 23 (7) ◽

pp. 645-649 ◽

Cited By ~ 3

Author(s):

José H.E.S. Freitas ◽

Keissy V. de Santana ◽

Pollyanna M. da Silva ◽

Maiara C. de Moura ◽

Luana C.B.B. Coelho ◽

...

Keyword(s):

Aqueous Solutions ◽

Moringa Oleifera ◽

Moringa Oleifera Seed

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Mass Spectrometry-Based Identification of Urinary Antimicrobial Peptides in Dairy Cows

Protein and Peptide Letters ◽

10.2174/0929866526666191025105038 ◽

2020 ◽

Vol 27 (3) ◽

pp. 225-235

Author(s):

Ambika Sharma ◽

Rajesh Nigam ◽

Ashish Kumar ◽

Simmi Singh

Keyword(s):

Mass Spectrometry ◽

Antimicrobial Peptides ◽

Polyacrylamide Gel Electrophoresis ◽

Radial Diffusion ◽

Protein Database ◽

Antimicrobial Effects ◽

Cow Urine ◽

Radial Diffusion Assay ◽

Promising Source ◽

Diffusion Assay

Background:: Urine is considered one of the biological fluids in which antimicrobial peptides are secreted or expressed. Cow urine has not been investigated for the presence of these peptides using MALDI-TOF-MS. Objective:: The aim of this study is to isolate, identify and assess the antimicrobial activity of urinary antimicrobial peptides from healthy normal cycling cows. Method:: We analyzed the urine sample using diafiltration, ion exchange chromatography, Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), acid urea polyacrylamide gel electrophoresis (AU-PAGE) coupled with identification through Peptide Mass Fingerprinting (PMF) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOF- MS). The in vitro antimicrobial effects of purified fractions were assessed using Radial Diffusion Assay (RDA) and microtitre broth dilution assay against Gram-positive and Gramnegative bacteria. Results: : Proteins corresponding to the peaks were identified using SWISSPROT protein database. This study revealed constitutive expression of β-Defensin-1 (DEFB1), β-Defensin-4A (DFB4A), Neutrophil Defensin-1 (DEF1), Neutrophil Defensin-3 (DEF3) in cow urine. The identified peptides are cationic antimicrobial peptides of the defensin family. The purified fractions exhibited antimicrobial effects in radial diffusion assay and MIC values in the range of 2.93-29.3 &*#181;M/L. Conclusion:: This study concludes that cow urine, previously unexplored with regard to antimicrobial peptides, would be a promising source of highly potent AMPs and an effective alternative to the resistant antibiotics.

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