Survei Infestasi Lalat Stomoxys spp. Pada Sapi Bali di Wilayah Kabupaten Badung

Buletin Veteriner Udayana ◽

10.24843/bulvet.2021.v13.i02.p14 ◽

2021 ◽

pp. 217

Author(s):

Dhea Septiany Peda Lalupada ◽

I Made Dwinata ◽

Ida Bagus Made Oka

Keyword(s):

Anaplasma Marginale ◽

Trypanosoma Evansi ◽

Besnoitia Besnoiti ◽

Cross Sectional

Sapi bali merupakan ternak yang banyak dipelihara oleh masyarakat di Kabupaten Badung. Lalat Stomoxys spp. merupakan salah satu ektoparasit penghisap darah pada sapi dan juga berperan sebagai vektor dari beberapa penyakit seperti rickettsia (anaplasma marginale), surra (trypanosoma evansi), besnoitiosis (besnoitia besnoiti). Penelitian ini bertujuan untuk mengetahui prevalensi lalat Stomoxys spp. pada sapi bali di Kabupaten Badung, serta hubungan cara pemeliharaan, kondisi wilayah, jenis kelamin dan umur terhadap infestasi lalat Stomoxys spp. Sampel penelitian ditentukan secara purposive dengan jumlah sampel 300 ekor sapi bali. Penelitian ini merupakan penelitian observasional dengan rancangan Cross-sectional. Hasil penelitian menunjukkan bahwa prevalensi infestasi lalat Stomoxys spp. di Kabupaten Badung adalah 50,3%. Prevalensi infestasi lalat Stomoxys spp. pada ternak yang dikandangkan (59%) lebih tinggi terinfestasi lalat Stmoxys spp. dibandingkan dengan ternak yang diikat (33%), sedangkan pada wilayah lahan basah (59%) lebih tinggi prevalensinya dibandingkan wilayah lahan kering (33%). Prevalensi ternak muda (51,4 %) paling tinggi terinfestasi lalat Stomoxys spp. dibandingkan dengan ternak dewasa (50%) dan ternak tua (45.5 %), prevalensi infestasi lalat Stomoxys spp. pada ternak betina (58,1 %) lebih tinggi dibandingkan ternak jantan (35,3%). Faktor kondisi wilayah, cara pemeliharaan dan jenis kelamin sangat berpengaruh (P<0,05) terhadap infestasi lalat Stomoxys spp. pada sapi bali di Kabupaten Badung, namun umur tidak berpengaruh (P>0,05).

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Serological and molecular survey of Trypanosoma evansi in Camels (Camelus dromedarius) in Maiduguri, North-east, Nigeria

Nigerian Journal of Parasitology ◽

10.4314/njpar.v42i1.8 ◽

2021 ◽

Vol 42 (1) ◽

pp. 56-62

Author(s):

S.A. Mamman ◽

G. Abongaby ◽

O. Salami ◽

J.P. Yidawi ◽

D.A. Dakul

Keyword(s):

Diagnostic Techniques ◽

Trypanosoma Evansi ◽

Surface Glycoprotein ◽

Dromedary Camel ◽

Serological Method ◽

Cross Sectional ◽

North East ◽

Variable Surface ◽

Pcr Targeting ◽

Pcr Techniques

To date, camels still remain an important work animal as well as source of protein to humans in the Sudan and Sahel regions of Nigeria. Therefore, a cross-sectional study was conducted on 150 camels slaughtered in Maiduguri central abattoir to determine the prevalence of Trypanosoma evansi using Card Agglutination Test (CATT) and Polymerase Chain Reaction (PCR) techniques. Overall, 30 (20%) of the camels tested were seropositive while PCR targeting the 227 base pair of the Variable Surface Glycoprotein (VSG) gene of T. evansi detected the DNA of the parasite in 9 out of the 30seropositive camels. Higher infection was found among adult compared to the young camels using the two diagnostic techniques; 24.1% vs 19.0% and 10.3% vs 4.6%, for CATT and PCR techniques, respectively. However, the differences being not statistically significant (P > 0.05) for the two methods of diagnosis. Furthermore, significantly (P < 0.05)higher prevalence of infection was recorded among male compared to female camels using the serological method of diagnosis, while (P > 0.05) using the molecular method; 27.5% vs 13.6% for CATT and 10.1% vs 2.5% for PCR. Camels with PCV =24 %( mean: 19.8923 ± 4.0931) recorded significantly (P < 0.05) higher prevalence of 23.1% than those with PCV = 25% (mean 31.7294 ± 5.50584), where the prevalence was 17.6%.The results of this study showed that camel trypanosomosis is endemic in the study area. Furtherstudiesto elucidate the epidemiology and socioeconomic impact of this disease in the northeast region of Nigeria are desirable. Keywords:Serology, PCR, Dromedary camel, T.evansi, Maiduguri

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Seroprevalence of Babesia bovis , B. bigemina , Trypanosoma evansi , and Anaplasma marginale antibodies in cattle in southern Egypt

Ticks and Tick-borne Diseases ◽

10.1016/j.ttbdis.2016.10.008 ◽

2017 ◽

Vol 8 (1) ◽

pp. 125-131 ◽

Cited By ~ 14

Author(s):

Ragab M. Fereig ◽

Samy G.A. Mohamed ◽

Hassan Y.A.H. Mahmoud ◽

Mahmoud Rezk AbouLaila ◽

Azirwan Guswanto ◽

...

Keyword(s):

Anaplasma Marginale ◽

Trypanosoma Evansi ◽

Babesia Bovis

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Estimating the impact of Trypanosoma evansi infection (surra) on buffalo population dynamics in southern Philippines using data from cross-sectional surveys

International Journal for Parasitology ◽

10.1016/j.ijpara.2009.02.012 ◽

2009 ◽

Vol 39 (10) ◽

pp. 1109-1114 ◽

Cited By ~ 28

Author(s):

A.P. Dargantes ◽

R.T. Mercado ◽

R.J. Dobson ◽

S.A. Reid

Keyword(s):

Population Dynamics ◽

Trypanosoma Evansi ◽

Cross Sectional ◽

Using Data ◽

The Impact

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Seroprevalence of Trypanosoma evansi infections among dromedary camels (Camelus dromedaries) in North Al-Sharqiya governorate, Sultanate of Oman

Journal of Agricultural and Marine Sciences [JAMS] ◽

10.53541/jams.vol26iss2pp51-55 ◽

2021 ◽

Vol 26 (2) ◽

pp. 51-55

Author(s):

A.H. Al-Kharusi ◽

E. I. Elshafie ◽

K.E. M. Ali ◽

R. AL-Sinadi ◽

Baniuraba N. ◽

...

Keyword(s):

Risk Factors ◽

Binary Logistic Regression ◽

Trypanosoma Evansi ◽

Sultanate Of Oman ◽

Dromedary Camels ◽

Serum Samples ◽

Cross Sectional ◽

Significant Difference ◽

Circulating Antibodies ◽

Associated Risk Factors

Trypanosoma evansi is a well-known hemoprotozoa that infects diverse domestic and wild animals worldwide and caused devastating disease called surra. This research aimed to investigate the seroprevalence of Trypanosoma evansi and its associated risk factors in dromedary camels in North Al-Sharqiya governorate in the Sultanate of Oman. A total of 4364 serum samples was collected from three willayat in north governorates of Sultanate of Oman; Ibra (926, 21.2%), Qabil (1119, 25.6%) and Bidiyah (2319, 53.1%). Samples were examined for the presence of antibodies against T. evansi using card agglutination test (CATT/T. evansi). Binary logistic regression was used to study the association of T. evansi seroprevalence and risk factors such as location, gender, purpose and age of camels. The overall seroprevalence of T. evansi detected by CATT/T. evansi test was 38% (1659/4364, CI: 36.6-39.5%). There was a significant difference (p=0.001) between location and T. evansi seroprevalence, whereas highest seroprevalence was found in Ibra (49.9%, CI: 46.7-53.1%) followed by Bidiyah (35%, CI: 33-36.9%) and Qabil (34.5%, CI: 31.8-37%). Camels from Ibra were almost two times more likely to have circulating antibodies of T. evansi than camels from Bidiyah (OR=1.89, CI: 1.591-2.168). Also, the results showed a significant difference between seroprevalence and sex (p=0.023), whereas the age of camels was not (p>0.05). To our knowledge, this is the first research that indicated that T. evansi antibodies were circulating among camels in Oman, and further research needs to be tackled to study the molecular characterization of T. evansi and its prevalence in other animal species. Furthermore, cross-sectional studies of T. evansi from different regions in Oman warrant further investigation.

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Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638718798688 ◽

2018 ◽

Vol 30 (6) ◽

pp. 942-945 ◽

Cited By ~ 3

Author(s):

Abdullah D. Alanazi ◽

Robert Puschendorf ◽

Bashir Salim ◽

Mohamed S. Alyousif ◽

Ibrahim O. Alanazi ◽

...

Keyword(s):

Saudi Arabia ◽

Internal Transcribed Spacer ◽

Molecular Detection ◽

Cross Sectional Study ◽

Trypanosoma Evansi ◽

Type B ◽

Sectional Study ◽

Internal Transcribed Spacer Region ◽

Cross Sectional ◽

Species Specific

We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species–specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses ( n = 12; 3.3%) and donkeys ( n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation.

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Detection and assessment of risk factors associated with natural concurrent infection of Trypanosoma evansi and Anaplasma marginale in dairy animals by duplex PCR in eastern Punjab

Tropical Animal Health and Production ◽

10.1007/s11250-014-0710-6 ◽

2014 ◽

Vol 47 (1) ◽

pp. 251-257 ◽

Cited By ~ 16

Author(s):

Amrita Sharma ◽

Lachhman Das Singla ◽

Ashuma Tuli ◽

Paramjit Kaur ◽

Mandeep Singh Bal

Keyword(s):

Risk Factors ◽

Anaplasma Marginale ◽

Trypanosoma Evansi ◽

Concurrent Infection ◽

Duplex Pcr ◽

Factors Associated ◽

Dairy Animals

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Prevalensi Infestasi Caplak Boophilus sp. pada Sapi Bali di Kabupaten Badung

Buletin Veteriner Udayana ◽

10.24843/bulvet.2021.v13.i01.p15 ◽

2021 ◽

pp. 99

Author(s):

Chairannisa Rustam ◽

I Made Dwinata ◽

Nyoman Adi Suratma

Keyword(s):

Anaplasma Marginale ◽

Babesia Bovis ◽

Cross Sectional

Sapi bali merupakan plasma nutfah asli Indonesia yang mempunyai keunggulan tahan hidup pada lingkungan yang kurang memadai, namun sapi bali juga dapat terjangkit penyakit salah satunya ektoparasit yaitu caplak. Caplak sapi atau Boophilus sp. adalah ektoparasit pengisap darah sehingga menyebabkan anemia pada ternak tersebut. Selain mengisap darah, Boophilus sp. juga merupakan vektor berbagai penyakit parasit darah diantaranya penyakit Babesiosis (Babesia bovis dan B. bigemina) dan Anaplasmosis (Anaplasma marginale). Faktor yang dapat mempengaruhi keberadaan caplak Boophilus sp. pada sapi yaitu umur, jenis kelamin, pemeliharaan, dan lingkungan. Penelitian ini bertujuan untuk mengetahui besarnya prevalensi dan predileksi yang menginfestasi sapi bali di Kabupaten Badung, serta hubungan faktor jenis kelamin, umur, cara pemeliharaan dan lingkungan terhadap infestasi caplak Boophilus sp. Sampel penelitian diambil di Kabupaten Badung, ditentukan secara purposive dengan jumlah sampel 300 ekor sapi bali. Penelitian ini merupakan penelitian observasional dengan rancangan Cross-sectional. Hasil penelitian menunjukkan bahwa prevalensi infestasi caplak Boophilus sp. di Kabupaten Badung adalah 7,7 %. Predileksi ditemukan pada bagian kaki (40 %), ambing (25 %), abdomen (17 %), punggung (12 %), dan kepala (6 %). Faktor umur, cara pemeliharaan dan lingkungan sangat berpengaruh (P<0,05) terhadap infestasi caplak Boophilus sp. pada sapi bali di Kabupaten Badung, namun jenis kelamin tidak berpengaruh (P>0,05).

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