scholarly journals PENGARUH KONSENTRASI ETANOL TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK RIMPANG ILALANG (Imperata cylindrica (L) Beauv.) PADA EKSTRAKSI MENGGUNAKAN GELOMBANG ULTRASONIK

2019 ◽  
Vol 8 (1) ◽  
pp. 27
Author(s):  
Corry Permatasari Suhendra ◽  
I Wayan Rai Widarta ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Ultrasonic Wave ◽  
Design Method ◽  
Imperata Cylindrica ◽  
Total Phenolic ◽  
Randomized Design ◽  
Ic50 Value ◽  
Completely Randomized Design ◽  
Gallic Acid Equivalent

This research porpose to determine the appropiate concentration of ethanol to obtain the highest antioxidant activity of cogon grass rhizome (Imperata cylindrica (Linn.) Beuv.) extract using ultrasonic wave. This research used a completely randomized design method with the treatment of concentration ethanol which consisted of six levels : 40, 50, 60,70, 80 and 90%. The whole treatment was repeated three times to obtain 18 units of the experiment. The data were analyzed by ANOVA, if the treatment showed the influenced then it continued by Duncan test. The result showed that the best treatment is using ethanol 70% with characteristic extract : yield was 14.13%, total phenolic was 129.57 mgGAE (Gallic Acid Equivalent)/g extract, total flavonoid was 90.91 mgQE (Quercetin Equivalent)/g extract and antioxidant activity was 56.03% with the IC50 value 0.098 mg/ml

scholarly journals Antioxidant Activity of Averrhoa bilimbi Linn. Leaves Extract Using Two Different Types of Solvents

2021 ◽  
Author(s):  
Raja Nurul Ashiqin Raja Arifin ◽  
Juliana Jumal
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Skin Diseases ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Natural Antioxidant ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Different Types ◽  
Gallic Acid Equivalent

Exposed to the pollution has led to generation of reactive oxygen species (ROS) in human skin. ROS generated cause many skin diseases such as skin-aging, inflammation, melanogenesis and skin cancer. ROS is a family of oxygen-based free radicals that contains or capable of producing an unpaired electron. Antioxidant is a molecule that can inhibit the reaction of free radical from ROS by donating its electron. Averrhoa bilimbi Linn. (AVBL) is one of the potent natural antioxidant belongs to the group of Oxalidaceae which can be widely found in Asia including Malaysia. Traditionally, this plant has been used to treat many diseases such as cough, itchiness, pimple, fever and inflammation. As a result, much attention has been directed towards the studies regarding the potential of this plant in treating disease. The present study was undertaken to assess the antioxidant activity of AVBL leaves extract. The AVBL leaves were extracted using sonicator with ethanol and distilled water as two different types of solvent. The total phenolic content (TPC) and flavonoid content (TFC) of this study were determined by using Folin-Ciocalteu reagent and aluminium chloride colometric assay. Antioxidant activity of the plant extract was tested using 2,2- diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing power (FRAP). From the analyses, water extract of AVBL possessed greater extraction yield (11.231%) as compared to ethanolic extract (5.358 %). However, ethanolic extract of AVBL leaves revealed higher result of TPC (126.4±0.35 mg/g gallic acid equivalent), TFC (32.80±0.37 mg/g quercetin equivalent), DPPH (0.0019±0.0003) and FRAP (41.81±0.45 mg/g gallic acid equivalent). The results of TPC and TFC have strongly positive correlation with antioxidant capacity (r = 1). Thus, it can be concluded that this plant is a potent source of natural antioxidant.

Stability of Black Garlic Extract on Various pH and Temperature

2020 ◽  
Vol 7 (1) ◽  
pp. 17
Author(s):  
Putu Eka Yudhayanti ◽  
I Dewa Gde Mayun Permana ◽  
Komang Ayu Nocianitri
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Garlic Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Processing Temperature ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
New Compounds

Black garlic is a garlic that experiences warming at a certain temperature and time to find higher antioxidant activity. This research aimed to know effect of antioxidant content’s stability of extract BG to pH and temperature. The experimental design used in this research was a completely randomized design with replication, which consisted of two factors. The first factor was pH (4, 6 and 8). The second factor was temperature (30, 50 and 70ºC). Data were analyzed with analysis of variance, followed by Duncan test. The results showed that pH and processing temperature very influential to antioxidant activity, total phenolic and total flavonoid. The results showed that the best treatment was pH 4 and temperature 30ºC which the best resulted antioxidant activity, total phenolic and total flavonoid that stablest. The resulted of antioxidant activity, stability of total phenolic and total flavonoid that were 75,13%, 42,47% and 77,71%. The treatment of pH and processing temperature change compounds of extract black garlic. Myricetin and quercetin appeared on treatment pH 4 with temperature 30ºC and pH 8 with temperature 70 ºC. The treatment of pH and processing temperature of black garlic extract reduced antioxidant activity, total phenol and total flavonoid. pH and temperature form new compounds.

scholarly journals PENGARUH SUHU PENGERINGAN TERHADAP KANDUNGAN KOMPONEN BIOAKTIF DAN KARAKTERISTIK SENSORIS TEH WHITE PEONY

2019 ◽  
Vol 8 (1) ◽  
pp. 36
Author(s):  
Dewa Ayu Tri Ulandari ◽  
Komang Ayu Nocianitri ◽  
Ni Made Indri Hapsari Arihantana
Keyword(s):  
Antioxidant Activity ◽  
Temperature Treatment ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Duncan Multiple Range Test ◽  
Drying Temperature ◽  
Randomized Design ◽  
Bioactive Component ◽  
Completely Randomized Design ◽  
Range Test

This study aims to determine the effect of drying temperature on the bioactive component contents of white peony tea and to know the proper drying temperature in white peony tea to produce the highest bioactive component contents and the best sensory characteristics. The experimental design used was Completely Randomized Design with a drying temperature as the treatment consisting of five levels: 60oC, 70oC, 80oC, 90oC, and 100oC. The treatment was repeated 3 times to obtain 15 experiment units. The data obtained were analyzed by variance and if the treatment had an effect on the observed variable then continued with The Duncan Multiple Range Test. The results showed that the 90oC drying temperature treatment produced white peony tea with the highest bioactive component contents and the best sensory characteristics with water content 4,06% (wet basis), yield 52,54%, total phenolic 211,01 mg GAE/g tea, flavonoid 10,90 mg CE/g tea, catechin 3,55 mg/g tea, antioxidant activity 50,20%, brew color yellow and liked, astringent taste and usual, aroma liked and overall acceptance rather liked.

scholarly journals Zinc as promoter of growth and biochemical activity in basil cultivars under in vitro conditions

2019 ◽  
pp. 342
Author(s):  
Caroline Galego Comar ◽  
Edinara Maria Barbosa ◽  
Vanusa Souza Rocha Pereira ◽  
Julliane Destro de Lima ◽  
Thiago Teodoro Santana ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Culture Medium ◽  
Total Phenolic ◽  
In Vitro Cultivation ◽  
Biochemical Activity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

In vitro cultivation of basil allows the manipulation of the concentration of certain micronutrients, commonly neglected by the micropropagation protocols. It is a plant of great economic importance for the cosmetic and pharmaceutical industry, due to the components present in its essential oil. In view of the above, the objective of this study was to evaluate zinc (Zn) concentrations in the micropropagation of basil, in addition to antioxidant activity and total phenolic compounds. Basil seeds, cultivars Manolo and Grecco Palla were oxygenated for 4 h, passed through asepsis and placed in test tubes with MS medium supplemented with 30 g L-1 sucrose and 6.5 g L-1 agar and pH adjusted to 5.8. The treatments were composed by the addition or not of 25 μM of zinc sulfate (ZnSO4) and arranged in a completely randomized design. The tubes containing the seeds and the culture medium were kept in a growth chamber for 90 days. The cultivar Manolo was more sensitive to the addition of ZnSO4 due to the increase in the number of leaves and in the antioxidant activity, however, the addition of this component in the culture medium did not influence the production of phenolic compounds or the activity of the antioxidant enzymes SOD, CAT and APX.

scholarly journals Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Daun Kejompot (Crassocephalum crepidioides) Menggunakan Metode Maserasi

2021 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Maria Christinela Domithesa ◽  
I Nengah Kencana Putra ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Design Method ◽  
Total Flavonoid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Right ◽  
Range Test ◽  
Treatment Water

This study aims to determine the effect of various solvent towards antioxidant activity of kejompot (crassocephalum crepioides) extract and to know the right solvent to obtained extract with the highest antioxidant activity. This study used a Completely Randomized Design Method with 4 treatment (water, methanol 70%, ethanol 70%, acetone 70%,) using maseration method. The treatment was repeated four times to obtain 16 experimental units. If there was a significant effect on the observed parameters, then followed by Duncan multipple range test. The result showed that the treatment significantly effected (P<0.05) on each of parameters such as : yield, tanin total, flavonoid, vitamin C, antioxidant activity. This study showed that methanol 70% as the best treatment that obtained yield, tannin, flavonoid, Vitamin C and antioxidant activity respectively 18.76 %, 1.05 mg TAE/g, 7.59 mg QE/g, 8.68 mg AAE/g, and 103.1 ppm antioxidant activity based on IC50  .

scholarly journals PENGARUH JENIS PELARUT DAN RASIO BAHAN DENGAN PELARUT TERHADAP KANDUNGAN SENYAWA FENOLIK DAN AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI ALPUKAT (PERSEA AMERICANA MILL.)

2018 ◽  
Vol 7 (2) ◽  
pp. 22
Author(s):  
Ginanjar Rifai ◽  
I Wayan Rai Widarta ◽  
Komang Ayu Nocianitri
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Analysis Of Variance ◽  
Persea Americana ◽  
Total Phenolic ◽  
Experiment Data ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Avocado Seed

This research was conducted in order to determine the type of solvent and ratio between sample and solvent to produce extract avocado seed with the highest antioxidant activity. The experimental design used in this research was a factorial completely randomized design which consisted of two factors. The first factor was the type of solvent consisting of ethanol, methanol, and acetone. The second factor was ratio between sample and solvent of 1:5, 1;10, and 1:15. The treatment was repeated three time to obtain 27 units of the experiment. Data were analyzed with analysis of variance, followed by Duncan test. The results show that the solvent of acetone and ratio between sample and solvent of 1:15 was highest antioxidant activity with total phenolic 803.60 mg/100g, IC50 was 540.95 ppm, and yield 41.36%.

scholarly journals PENGARUH LAMA EKSTRAKSI MENGGUNAKAN ULTRASONIK TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN TEMPUYUNG (Sonchus arvensis L.)

2020 ◽  
Vol 9 (3) ◽  
pp. 321
Author(s):  
Ni Made Miradita Lestari ◽  
Ni Made Yusa ◽  
Komang Ayu Nocianitri
Keyword(s):  
Antioxidant Activity ◽  
Ultrasonic Method ◽  
Ethanol Extract ◽  
Ring Test ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Sonchus Arvensis ◽  
Extraction Treatment

This study was conducted to determine the effect of time extraction using ultrasonic method on the antioxidant activity of tempuyung leaves ethanol extract and to find out the exact time to get tempuyung leaves ethanol extract with the highest antioxidant activity. The experimental design used in this study was a completely randomized design with time extraction as a treatment. The treatment consisting of five levels, which is 10 minutes, 20 minutes, 30 minutes, 40 minutes, 50 minutes using ultrasonic method with temperature 45oC and frequency 37 kHz. All treatments were repeated three times so that they were obtained 15 experimental units. The data obtained were analyzed of variance and if the treatment had significant effect followed by Duncan’s Multiple Ring Test. The results showed that the time extraction treatment had a very significant effect (P<0.01) on yield, total phenol, total flavonoids, total tannin, and antioxidant activity from tempuyung leaves ethanol extract. The best treatment was 30 minutes which produced highest antioxidant activity of 58,60 % with IC50 262,82 mg/L included in the weak category, yield 18,60%, total phenolic 55,05 mg GAE/g, total flavonoid 38,14 mg QE/g, total tannin 8,82 mg TAE/g.

scholarly journals SIFAT FISIK DAN ORGANOLEPTIK MIE DARI TEPUNG TALAS (Colocasia esculenta) DAN TERIGU DENGAN PENAMBAHAN SARI BAYAM MERAH (Amaranthus blitum)

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Meiheski R. Rara ◽  
Teltje Koapaha ◽  
Dekie Rawung
Keyword(s):  
Antioxidant Activity ◽  
Wheat Flour ◽  
Cooking Time ◽  
Cooking Loss ◽  
Water Absorbency ◽  
Average Value ◽  
Randomized Design ◽  
Taro Flour ◽  
Ic50 Value ◽  
Completely Randomized Design

ABSTRACT This study aims to evaluate the proportion of taro flour to produce the best noodles based on the physical characteristics and preferences by panelists. This study uses a completely randomized design with 4 treatments A (100% wheat flour) as a control, B (80% taro flour: 20% wheat flour), C (70% taro flour: 30% wheat flour), D (60% taro flour: 40% wheat flour). The parameters analyzed were water absorption, elasticity, cooking time, cooking loss, moisture content, antioxidant activity and acceptance level of the panelists. From the results of the research on testing the physical properties of taro noodles with water absorbency an average value of 90.40%, elasticity obtained an average value of 15.87%, cooking time obtained an average value of 210.67 seconds, cooking loss obtained value on average 21.37% and water content obtained an average value of 24.83%. Meanwhile, the antioxidant activity of taro noodles is classified as weak, namely the IC50 value is 225.82 µg / ml. For organoleptic tests, the average panelist gave likes value for color and neutral for smell, but they did not like the taste and texture of wet noodles made from taro flour with the addition of red spinach juice. Keywords: Taro, red spinach, wet noodles

scholarly journals Study of Total Phenolic, Total Flavonoid, Scopoletin Contents and Antioxidant Activity of Extract of Ripened Noni Juice

2021 ◽  
Vol 23 (2) ◽  
pp. 55-62
Author(s):  
Lia Meilawati ◽  
Teni Ernawati ◽  
Rizna Triana Dewi ◽  
Megawati Megawati ◽  
Sukirno Sukirno
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Water Solvent ◽  
Activity Test ◽  
Gallic Acid Equivalent

AbstractExtraction of ripened noni juice has been carried out using ethanol and water. The purpose of this study was to determine the total phenolic, total flavonoids, scopoletin, content and antioxidant activity of the ripened noni juice extract. The activity test was carried out on the ripened noni juice extract without and with the addition of ethanol and water solvent. The phytochemical assays of ethanol extract of the ripened noni juice showed that thetotal phenolic content was 3,94 mg gallic acid equivalent/g extract, the total flavonoids was 0,59 mg quercetin equivalent /g extract, the IC50 antioxidant activity was 24,92 mg/L, scopoletin content was 2,45 mg/gram.Keywords : Antioxidant, flavonoids, phenolic, ripened noni juice, scopoletin

scholarly journals PENGARUH JENIS PELARUT PADA EKSTRAKSI MENGGUNAKAN GELOMBANG ULTRASONIK TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BUAH LEMON (Citrus limon (Linn.) Burm F.)

2018 ◽  
Vol 7 (4) ◽  
pp. 213
Author(s):  
Melia Verdiana ◽  
I Wayan Rai Widarta ◽  
I Dewa Gede Mayun Permana
Keyword(s):  
Antioxidant Activity ◽  
Citrus Limon ◽  
Experiment Data ◽  
Effect Of Solvent ◽  
Lemon Peel ◽  
Randomized Design ◽  
Ic50 Value ◽  
Completely Randomized Design ◽  
The Right ◽  
Peel Extract

This study aims to determine the effect of solvent types on antioxidant activity of lemon peel (Citrus limon (Linn.) Burm F.) extract and to obtain the right solvent types with the highest antioxidant activity of lemon peel extract. The experimental design used in this research  was a completely randomized design with the treatment of solvent types (aquades, acetone 70%, ethanol 70% and methanol 70%). The treatment  was repeated four times to obtain 16 units of the experiment. Data were analyzed with analysis of variance (ANOVA) and followed by Duncan test. The result showed that the best treatment was obtained extract with ethanol 70% solvent with antioxidant activity was 52.72% and the IC50  value 471.33 mg/L, followed by yield was 37.68%, vitamin C was 227.90 mg AAE/g extract and total flavonoid was 7.14 mg QE/g extract.

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