scholarly journals Isolasi dan Karakterisasi Bakteri Penghasil Etanol dari Lingkungan Industri Arak Bali di Desa Merita dan Tri Eka Buana, Karangasem-Bali

2020 ◽  
Vol 8 (2) ◽  
pp. 279
Author(s):  
Azis Akbar Hakim ◽  
I M. Mahaputra Wijaya ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Fermentation Process ◽  
Visible Spectrophotometry ◽  
Gram Positive Bacteria ◽  
Production Location ◽  
Coconut Tree ◽  
Isolation And Characterization ◽  
Exposure Method ◽  
Sucrose Medium ◽  
Uv Visible

The purpose of this research is isolation and characterization to find potential bacteria which can produce the most optimal ethanol from the Arak Bali industry in Karangasem Regency, Bali. Bacteria were isolated by exposure method in open air using selective media Zymomonas Sucrose Medium (ZSMA) with the addition of nystatin as much as 0.18 g / L as an antifungal then samples were taken at three different points in one Arak Bali production location, namely the distillation place, the fermentation room for roomie, and the place of taking coconut juice under the coconut tree and the variation of time is 15, 30, and 60 minutes of exposure. Gas checking is done on the bacteria obtained to select its ability to produce ethanol. The results of the scanning of 11 best isolates using UV-visible spectrophotometry were fermented on 500 mL ZSM media for 10 days. BM1-CP14 is the best isolate to produce total ethanol of 15.33 mL through the fermentation process. The results of the characterization of BM1-CP14 isolates were Gram-positive bacteria in the form of bacilli, anaerobic and non-motile bacteria. The results showed that bacteria isolated from open-air also can produce ethanol. Keyword: ethanol, Arak Bali, airborne bacterial exposure, isolation, characterization, UV-Visible spectrophotometry

scholarly journals Isolasi dan Karakterisasi Bakteri Penghasil Bioetanol dari Lingkungan Industri Arak di Desa Tri Eka Buana, Kecamatan Sidemen, Karangasem Bali

2020 ◽  
Vol 8 (2) ◽  
pp. 290
Author(s):  
Awang Bagaskara ◽  
I Made Mahaputra Wijaya ◽  
Nyoman Semadi Antara
Keyword(s):  
Fermentation Process ◽  
Bacterial Isolate ◽  
Alcohol Oxidation ◽  
Gas Production ◽  
Growth Media ◽  
Oxidation Reactions ◽  
Visible Spectrophotometry ◽  
Gram Positive Bacteria ◽  
Coconut Tree ◽  
Uv Visible

This research aimed to determine the ability of each bacterial isolate to produce ethanol, isolate and characterize bacteria that have the ability to produce potential ethanol from lau and air in the Arak Industry of environment in the Tri Eka Buana Village, Sidemen District, Karangasem Bali. Samples were obtained from 3 Hamlets in Tri Eka Buana Village. Bacteria were taken from lau before fermentation, during fermentation, and after fermentation. Bacteria from air were taken from the fermentation, distillation, and under the coconut tree and the variation of time is 15, 30, and 60 minutes of exposure. Bacteria from lau were isolated by growing on growth media, periodically (after 30, 60, 90, 120, and 150 seconds) the isolates were taken from suspension to be inoculated in growth media for gas production. Isolation was carried out using YEPD-fluconazole media to obtain pure isolates. The fluconazole concentration used was 0,2 g/L. Pure isolates were then carried out qualitative tests for gas production and quantitative tests with alcohol oxidation reactions. The results of UV-Visible spectrophotometry, there were 6 best isolates and then fermented in YEPD media on a scale of 900 mL for 10 days. The fermentation results were then distilled using a reflux distilator. BU3.111E1 isolates are determind the best isolates with 8,50% brix ethanol. The BU3.111E1 were obtained from air bacteria in Pungutan Hamlet, place of sampling during the fermentation process for 15 minutes. From the characterization results, isolate BU3.111E1 was Gram positive bacteria, basil bacteria, motile bacteria, and catalase negative. This study proved that the bacteria had the ability to produce ethanol. Keyword: isolation, bioethanol, arak, bacteria, UV-Visible spectrophotometry

Isolation and characterization of jadomycin L from Streptomyces venezuelae ISP5230 for solid tumor efficacy studies

2009 ◽  
Vol 81 (6) ◽  
pp. 1041-1049 ◽  
Author(s):  
David L. Jakeman ◽  
Stephanie N. Dupuis ◽  
Cathy L. Graham
Keyword(s):  
Natural Products ◽  
Solid Tumor ◽  
Culture Media ◽  
Biological Activities ◽  
Streptomyces Venezuelae ◽  
Gram Positive Bacteria ◽  
Isolation And Characterization ◽  
One Step ◽  
Efficacy Studies

Precursor-directed biosynthesis offers opportunities to modify natural products and obtain structurally complex metabolites without the need for chemical synthesis. However, such opportunities are limited owing to the inherent substrate specificity of biosynthetic enzymes. The jadomycins are a family of natural products produced by the soil microbe Streptomyces venezuelae ISP5230. Their biosynthesis contains one step that is potentially non-enzymatic, namely, the condensation of a biosynthetic aldehyde and an amino acid that leads to a uniquely substituted oxazolone ring. Variation of amino acids in the culture media enables the production of a wide array of substituted oxazolones. These analogs have been shown to have a variety of biological activities against cancer cell lines and also against Gram-positive bacteria. Herein, we report the first isolation and characterization of jadomycin L and jadomycin L aglycone from 8 L of bacterial culture for solid tumor efficacy studies.

Isolation and characterization of Gram-negative and Gram-positive bacteria capable of producing piceatannol from resveratrol

2019 ◽  
Vol 103 (14) ◽  
pp. 5811-5820 ◽  
Author(s):  
Toshiki Furuya ◽  
Naoto Imaki ◽  
Kosuke Shigei ◽  
Masahiko Sai ◽  
Kuniki Kino
Keyword(s):  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF BIOACTIVE STREPTOMYCES FROM MANGROVE ECOSYSTEM OF MACHILIPATNAM, KRISHNA DISTRICT, ANDHRA PRADESH

2016 ◽  
Vol 9 (9) ◽  
pp. 258
Author(s):  
Yasmeen Saba ◽  
Vijayalakshmi Muvva ◽  
Rajesh Kumar Munaganti
Keyword(s):  
Mangrove Ecosystem ◽  
Bioactive Metabolites ◽  
Phylogenetic Study ◽  
Gram Negative Bacteria ◽  
Gram Positive Bacteria ◽  
Isolation And Characterization ◽  
Mangrove Soils ◽  
Taxonomic Studies ◽  
Streptomyces Genus

ABSTRACTObjective: The aim of the present study was to isolate, identify, and analyze the taxonomic characteristics of the potent actinobacterial strains VJSY-2and VJSY-3 isolated from mangrove soils of Gilakaladindi, Machilipatnam, Krishna District of Andhra Pradesh.Methods: Soil samples pretreated with calcium carbonate were used for isolation of potent actinobacterial strains. Identification of the strains wascarried out by studying the cultural, morphological, biochemical, and physiological characteristics. The phylogenetic study of the strains was carriedout by employing 16S r DNA sequence-based analysis. Phylogenetic tree was constructed using the molecular evolutionary genetic analysis softwareversion 6.Results: Based on the polyphasic taxonomic studies, the potent strains belong to Streptomyces genus. The bioactive metabolites produced by the strainswere active against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Bacillus megaterium), Gram-negative bacteria (Xanthomonascampestris, Pseudomonas aeruginosa, and Escherichia coli), and fungi (Aspergillus niger, Fusarium solani, Fusarium oxysporum, and Candida albicans).Conclusion: The results of the experiment showed that the crude ethyl acetate extract of the strains VJSY-2 and VJSY-3 showed significant antimicrobialpotential; hence, they can be used for isolation of compounds with pharmaceutical importance.Keywords: Mangrove Actinobacteria, Streptomyces, Bioactive metabolites.

scholarly journals Isolation and Analysis of Methanol Extract of Leaf of Senna siamea

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
V. M. Balami ◽  
J. Yakubu ◽  
J. Yakubu ◽  
U. T. Mamza ◽  
S. I. Dawa ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Methanol Extract ◽  
Retardation Factor ◽  
Ethyl Benzene ◽  
Senna Siamea ◽  
Isolation And Characterization ◽  
Ms Analysis ◽  
Uv Visible ◽  
Column Chromatographic

This study aimed at isolation and characterization of biochemical constituents of the leaf extract of Senna siamea. The fresh leaves of Senna siamea were air-dried, pulverized and extracted using methanol by maceration method. The extract was screened for phytochemicals using standard methods. Fifty grammas (50 g) of the crude extract was defatted using n-hexane; 20 g was subjected to column chromatographic (CC) analysis using ethyl acetate and n-butanol fractions with similar retardation factor (Rf) were pooled and coded. Subsequently purification of fraction SSM5 was carried out using CC with solvent [Toluene and n-butanol (4:1) as mobile phase]. The collection was based on colour bands) and TLC was carried out where a sub-fraction SSM54 amongst other fractions gave a single spot on TLC and the Rf value was 0.71. It was then subjected to UV, IR and GC-MS analysis. The extract by maceration yielded 12.60% w/w and the defatted extract yielded 10.0% w/w. The result of the UV-visible gave a wavelength of 289 nm and an absorbance of 4.268. The result of infrared spectroscopy revealed functional groups thus O-H, N-H, C-H stretching group at 3385.8 cm-1, -CHO at 2955.8 cm-1, -CHO stretching at 2926.0 cm-1,-NH3+ at 1640.0 cm-1, carboxylate ion–CO2- for amino acid at 1449.9cm-1, -N=N- at 1401.5 cm-1, -N+O-=N- at 1315.8 cm-, C-O-C at 1189 cm-1, Si-O at 1084 cm-1 and S=O at 1013.8. The GC-MS analysis of SSM54 revealed the presence of Diisoctyl phthalate, Purine-2,6-dione, 8-(3-Ethoxypropylamino)-1,3-dimethyl-3, 9-dihydro-, a-Pinene, 10-(dimethylaminomethyl), 1H-indene-2-ethanamine, N, N- dimethyl-3-[1-(2-pyrindinyl) ethyl] -, Benzene, 1, 2- bis(2,5 dimethylphenylaminomethyl)-3,6- dimethyl- and Cinnamic acid, p-(trimethylsiloxy)-, methyl ester were among the probable bioactive compounds. 

scholarly journals Isolation and Characterization of Lactic Acid Bacteria (Lactobacillus sp) from Tomato (Solanum lycopersicum)

BioScience ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 45
Author(s):  
Resti Fevria ◽  
Indra Hartanto
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Staining Method ◽  
Chemical Compositions ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Cell Form

Prebiotics are good coumpounds used in the growth of lactic acid bacteria (LAB) in digestion, prebiotics come from vegetables, fruits and seeds. Tomatoes are thought to be one of the natural media of bacteria because tomatoes contain various chemical compositions that are needed as a substrate for LAB. The purpose of this study is to isolate and determine the type of LAB produced microscopically. The ingredients used are tomatoes obtained from Padang Panjang market, with Merck NA media, oxoid MRSa, 0,9% NaCl, and crystal violet paint. Isolation LAB from tomatoes done in two ways, the first with plant tomato tissue directly into the NA medium and the second with fermentation tomato and then plant the tomato into the MRSa medium with streak plate methods. The isolates obtained were identified microscopically using a microscope with gram staining method. From the research that has been done, the following result are obtained : (1) Plant tomatoes tissue direcly into NA medium and gram staining, there were 1 colonies of gram positive bacteria with bacil cell form. (2). Fermentation tomatoes from the results, there were 2 colonies gram positive bacteria with bacil cell form. We can identify this colonies as Lactobacillius bulgaricus.

scholarly journals Antimicrobial Activity of Carbon Nanoparticles Isolated from Natural Sources against Pathogenic Gram-Negative and Gram-Positive Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sheena Varghese ◽  
Sunny Kuriakose ◽  
Soumya Jose
Keyword(s):  
Carbon Nanoparticles ◽  
Bacterial Strains ◽  
Visible Spectroscopy ◽  
Gram Positive ◽  
Natural Sources ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Inhibition Zones ◽  
Uv Visible

This paper describes the isolation of carbon nanoparticles (CNPs) from kitchen soot, characterization of the CNPs by UV/visible spectroscopy, SEM and XRD, and their antimicrobial action. The antibacterial activity of the isolated carbon nanoparticles was tested against various pathogenic bacterial strains such as Gram-negative Proteus refrigere and Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus and Streptococcus haemolyticus. The inhibition zones were measured, and it was found that the carbon nanoparticles isolated from natural sources are active against these Gram-negative and Gram-positive bacterial strains.

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