scholarly journals Isolation and Analysis of Methanol Extract of Leaf of Senna siamea

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
V. M. Balami ◽  
J. Yakubu ◽  
J. Yakubu ◽  
U. T. Mamza ◽  
S. I. Dawa ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Methanol Extract ◽  
Retardation Factor ◽  
Ethyl Benzene ◽  
Senna Siamea ◽  
Isolation And Characterization ◽  
Ms Analysis ◽  
Uv Visible ◽  
Column Chromatographic

This study aimed at isolation and characterization of biochemical constituents of the leaf extract of Senna siamea. The fresh leaves of Senna siamea were air-dried, pulverized and extracted using methanol by maceration method. The extract was screened for phytochemicals using standard methods. Fifty grammas (50 g) of the crude extract was defatted using n-hexane; 20 g was subjected to column chromatographic (CC) analysis using ethyl acetate and n-butanol fractions with similar retardation factor (Rf) were pooled and coded. Subsequently purification of fraction SSM5 was carried out using CC with solvent [Toluene and n-butanol (4:1) as mobile phase]. The collection was based on colour bands) and TLC was carried out where a sub-fraction SSM54 amongst other fractions gave a single spot on TLC and the Rf value was 0.71. It was then subjected to UV, IR and GC-MS analysis. The extract by maceration yielded 12.60% w/w and the defatted extract yielded 10.0% w/w. The result of the UV-visible gave a wavelength of 289 nm and an absorbance of 4.268. The result of infrared spectroscopy revealed functional groups thus O-H, N-H, C-H stretching group at 3385.8 cm-1, -CHO at 2955.8 cm-1, -CHO stretching at 2926.0 cm-1,-NH3+ at 1640.0 cm-1, carboxylate ion–CO2- for amino acid at 1449.9cm-1, -N=N- at 1401.5 cm-1, -N+O-=N- at 1315.8 cm-, C-O-C at 1189 cm-1, Si-O at 1084 cm-1 and S=O at 1013.8. The GC-MS analysis of SSM54 revealed the presence of Diisoctyl phthalate, Purine-2,6-dione, 8-(3-Ethoxypropylamino)-1,3-dimethyl-3, 9-dihydro-, a-Pinene, 10-(dimethylaminomethyl), 1H-indene-2-ethanamine, N, N- dimethyl-3-[1-(2-pyrindinyl) ethyl] -, Benzene, 1, 2- bis(2,5 dimethylphenylaminomethyl)-3,6- dimethyl- and Cinnamic acid, p-(trimethylsiloxy)-, methyl ester were among the probable bioactive compounds. 

scholarly journals Isolasi dan Karakterisasi Bakteri Penghasil Etanol dari Lingkungan Industri Arak Bali di Desa Merita dan Tri Eka Buana, Karangasem-Bali

2020 ◽  
Vol 8 (2) ◽  
pp. 279
Author(s):  
Azis Akbar Hakim ◽  
I M. Mahaputra Wijaya ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Fermentation Process ◽  
Visible Spectrophotometry ◽  
Gram Positive Bacteria ◽  
Production Location ◽  
Coconut Tree ◽  
Isolation And Characterization ◽  
Exposure Method ◽  
Sucrose Medium ◽  
Uv Visible

The purpose of this research is isolation and characterization to find potential bacteria which can produce the most optimal ethanol from the Arak Bali industry in Karangasem Regency, Bali. Bacteria were isolated by exposure method in open air using selective media Zymomonas Sucrose Medium (ZSMA) with the addition of nystatin as much as 0.18 g / L as an antifungal then samples were taken at three different points in one Arak Bali production location, namely the distillation place, the fermentation room for roomie, and the place of taking coconut juice under the coconut tree and the variation of time is 15, 30, and 60 minutes of exposure. Gas checking is done on the bacteria obtained to select its ability to produce ethanol. The results of the scanning of 11 best isolates using UV-visible spectrophotometry were fermented on 500 mL ZSM media for 10 days. BM1-CP14 is the best isolate to produce total ethanol of 15.33 mL through the fermentation process. The results of the characterization of BM1-CP14 isolates were Gram-positive bacteria in the form of bacilli, anaerobic and non-motile bacteria. The results showed that bacteria isolated from open-air also can produce ethanol. Keyword: ethanol, Arak Bali, airborne bacterial exposure, isolation, characterization, UV-Visible spectrophotometry

scholarly journals Isolation and Characterization of Hypolipidemic Compound from Cajanus cajan

2011 ◽  
Vol 24 (1) ◽  
pp. 6-10
Author(s):  
M Anwar Habib ◽  
ASM Anisuzzaman ◽  
RK Barman ◽  
M Abdul Gafur ◽  
M Tofazzal Haque
Keyword(s):  
Cajanus Cajan ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypolipidemic Activity ◽  
1H And 13C Nmr ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Biological Studies ◽  
Isolated Compound

The study was carried out to identify the compound responsible for hypolipidemic and hypoglycemic effects of Cajanus cajan (redgram). The methanol extract of redgram seeds was found to decrease fasting blood glucose and lipid profile (p<0.001) on streptozotocin-induced mice compared to control. This activity- guided fraction led to the isolation of a compound, substituted benzene containing polyhydroxy functions fused with lactone (CCA3) by analysis of 1H and 13C-NMR data. Biological studies of the isolated compound possessed prominent hypolipidemic activity. Although a number of hypoglycemic compounds are reported, yet not any hypolipidemic compound from redgram. The compound CCA3 seems to be the first report on hypolipidemic activity from methanol extract of redgram. TAJ 2011; 24(1): 6-10

scholarly journals Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer

2015 ◽  
Vol 2 (2) ◽  
pp. 86-98
Author(s):  
Dina Dyah Saputri ◽  
Maria Bintang ◽  
Fachriyan H Pasaribu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Lantana Camara ◽  
Rrna Gene ◽  
Antibacterial Compounds ◽  
Isolation And Characterization ◽  
Ms Analysis

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.

Two Antinematodal Phenolics from Knema hookeriana, a Sumatran Rainforest Plant

2000 ◽  
Vol 55 (3-4) ◽  
pp. 300-304 ◽  
Author(s):  
Yohannes Alen ◽  
Shuhei Nakajima ◽  
Teruhiko Nitoda ◽  
Naomichi Baba ◽  
Hiroshi Kanzaki ◽  
...  
Keyword(s):  
Effective Dose ◽  
Spectral Properties ◽  
Methanol Extract ◽  
Bursaphelenchus Xylophilus ◽  
Chromatographic Purification ◽  
Pine Wood ◽  
Cotton Ball ◽  
Isolation And Characterization ◽  
First Time

Abstract The activity-guided chromatographic purification of the methanol extract of Knema hookeriana, using pine wood nematodes Bursaphelenchus xylophilus has successfully led to the isolation and characterization of two phenolic antinematodal compounds with minimun effective dose (MED) of 4.5 and 20 μg/cotton ball (μg/ bl.) or 0.018 and 0.073 μᴍ/cotton ball (μᴍ/bl.), respectively. Based on their chemical and spectral properties, these compounds were determined to be 3-undecylphenol (1) and 3-(8Z-tridecenyl)-phenol (2). These compounds were isolated for the first time from this species, and 2 seems to be a novel compound.

scholarly journals Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion, Buthacus leptochelys

Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 236 ◽  
Author(s):  
Yusuke Yoshimoto ◽  
Masahiro Miyashita ◽  
Mohammed Abdel-Wahab ◽  
Moustafa Sarhan ◽  
Yoshiaki Nakagawa ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Scorpion Venom ◽  
Spectrometric Analysis ◽  
North African ◽  
High Sequence Similarity ◽  
The North ◽  
Isolation And Characterization ◽  
Ms Analysis ◽  
Insect Toxicity

Various bioactive peptides have been identified in scorpion venom, but there are many scorpion species whose venom has not been investigated. In this study, we characterized venom components of the North African scorpion, Buthacus leptochelys, by mass spectrometric analysis and evaluated their insect toxicity. This is the first report of chemical and biological characterization of the B. leptochelys venom. LC/MS analysis detected at least 148 components in the venom. We isolated four peptides that show insect toxicity (Bl-1, Bl-2, Bl-3, and Bl-4) through bioassay-guided HPLC fractionation. These toxins were found to be similar to scorpion α- and β-toxins based on their N-terminal sequences. Among them, the complete primary structure of Bl-1 was determined by combination of Edman degradation and MS/MS analysis. Bl-1 is composed of 67 amino acid residues and crosslinked with four disulfide bonds. Since Bl-1 shares high sequence similarity with α-like toxins, it is likely that it acts on Na+ channels of both insects and mammals.

Isolation and characterization of β-cadinene synthase cDNA from Chamaecyparis formosensis Matsum

Holzforschung ◽  
2012 ◽  
Vol 66 (5) ◽  
pp. 569-576 ◽  
Author(s):  
Pei-Min Kuo ◽  
Keng-Hao Hsu ◽  
Yi-Ru Lee ◽  
Fang-Hua Chu ◽  
Sheng-Yang Wang
Keyword(s):  
Functional Expression ◽  
Major Product ◽  
Gas Chromatography Mass Spectrometry ◽  
Synthesis Mechanism ◽  
E Coli ◽  
Isolation And Characterization ◽  
Ms Analysis ◽  
Chamaecyparis Formosensis ◽  
Rapid Amplification

Abstract Chamaecyparis formosensis is a precious conifer endemic in Taiwan. To understand the sesquiterpene synthesis mechanism in this tree, full-length cDNA of a putative sesquiterpene synthase (sesqui-TPS), designated Cf-Cad, was obtained by rapid amplification of complementary DNA ends-polymerase chain reaction. Cf-Cad is 1812 bp in length. To identify its function, recombinant protein from Escherichia coli was incubated with farnesyl diphosphate, which produced one major product, the structure of which was elucidated by gas chromatography/mass spectrometry (GC/MS) analysis. GC/MS analysis, GC retention time and MS matching with authentic standards revealed that the major product was β-cadinene. This is the first report of the cloning, functional expression in E. coli and identification of a sesqui-TPS from a Cupressaceae conifer.

scholarly journals Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn

2019 ◽  
pp. 1-10
Author(s):  
Halima A. Umar ◽  
Hamidu Usman ◽  
Mustapha B. Abubakar ◽  
Baba F. Mohammed ◽  
Mohammed Babakura ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanol Extract ◽  
Phyllanthus Amarus ◽  
Hydrolysable Tannin ◽  
Isolation And Characterization ◽  
Hydrolysable Tannins ◽  
Isolated Compound ◽  
Layer Chromatography

The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.

Isolation and Characterization of Cytotoxic Diterpenoid Isomers from Propolis

1997 ◽  
Vol 52 (9-10) ◽  
pp. 702-704 ◽  
Author(s):  
Tetsuya Matsuno ◽  
Yasuyuki Matsumoto ◽  
Masahiro Saito ◽  
Junji Morikawa
Keyword(s):  
Methanol Extract ◽  
Carcinoma Cell ◽  
Molecular Formula ◽  
Cell Cytotoxicity ◽  
Isolation And Characterization ◽  
Clerodane Diterpenoids ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell ◽  
Brazilian Propolis

The methanol extract of Brazilian propolis was fractionated by HPLC, based on HuH13 (human hepatocellular carcinoma) cell cytotoxicity assay. Two isomers of diterpenoid with a molecular formula of C20H30O3 (MW: 318.46) were isolated. The structures of these colorless compounds were determined as clerodane diterpenoids (I, 15-oxo-3, 13Z-kolavadien-17-oic acid; II, 15-oxo-3Z, 13E-kolavadien-n-oic acid)

Detection of Adulteration in Rubia cordifolia – A Chromatographic Approach

2021 ◽  
pp. 4013-4018
Author(s):  
Ghanshyam Kamani ◽  
Rohan Sanghani ◽  
Vaibhavi Savalia ◽  
Devang Pandya
Keyword(s):  
Mobile Phase ◽  
Methanol Extract ◽  
Skin Diseases ◽  
Chemical Constituents ◽  
Raw Material ◽  
Rubia Cordifolia ◽  
Methanol Extracts ◽  
Rubia Tinctorum ◽  
Ms Analysis ◽  
The One

According to traditional medicinal texts as well as modern research, Rubia cordifolia (Desi Manjistha) stem cure various diseases of blood, skin diseases, inflammations, kidney stones, fever and various Kapha disorders. However, stems of Rubia cordifolia (Desi Manjistha) are often adulterated with stems of Rubia tinctorum (Irani Manjistha) due to morphological similarity. This adulteration is extremely harmful as R. tinctorum has Lucidin as one of the chemical constituents, which has severe genotoxic effects. The present work focuses on developing a comparative HPTLC fingerprint and GC-MS analysis of R. cordifolia and R. tinctorum, so as to detect the adulteration by R. tinctorum in R. cordifolia raw material form and in formulations. Methanol extracts of the individually powdered stems were used to develop a suitable mobile phase, such that a clear spot was obtained in TLC of R. tinctorum but not in TLC of R. cordifolia using the same mobile phase. This mobile phase was further used to develop a comparative HPTLC fingerprint of the two species. Methanol extracts of R. cordifolia and R. tinctorum were used for investigation of phytoconstituents by GC-MS analysis. The mobile phase n-butanol: ethanol (6:4) was the one which gave a clear single spot at Rf 0.89 in TLC of methanol extract of R. tinctorum but not in TLC of methanol extract of R. cordifolia, at 254nm and 366nm. Further, HPTLC analysis gave results clearly differentiating the two species using the same mobile phase. Further, GC-MS analysis of R. cordioflia revealed the presence of 9 phytoconstituents while R. tinctorum revealed the presence of 6 phytoconstituents, which are different from each other. Thus, these simple yet sophisticated techniques will be very useful for herbal industry in standardization and detection of adulteration of R. tinctorum in R. cordifolia formulations, thereby benefitting the patients who consume Manjistha formulations.

scholarly journals Isolation and Characterization of New Compound and its Antibacterial Activities from Stem-Bark Extract of Barringtonia Asiatica

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Isaac John Umaru
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chromatographic Technique ◽  
Isolation And Characterization ◽  
Column Chromatographic

Objective: This investigation involves the extraction, isolation, and characterization of chemical constituents from a Barringtonia family plant, Barringtonia asiatica followed by Antibacterial, cytotoxicity and evaluation of its antioxidant principles. Methods: The dried stem-bark powders were subjected to sequential soaking with polar and nonpolar solvents and extraction using rota-vap. Dichloromethane extract reveals the presence of significant amount of phytochemicals. The dichloromethane extract was subjected to isolation using column chromatographic analysis with solvents such as, dichloromethane, chloroform, hexane, ethyl acetate and methanol. Further, the isolated compound was subjected to thin layer chromatography technique and spectral analysis such as infrared, 1H-NMR, 13C-NMR, FT-IR, and mass spectroscopy. The antibacterial using agar disc method. Results: The compound was isolated in hexane: ethyl acetate (9:1) and dichloromethane: ethyl acetate in the solvent system in the ratio of 9:1 and 7:3, respectively using column chromatographic technique. On the basis of phytochemical, chromatographic, and spectral analysis, the isolated compounds were identified as Nerolidiol and Heneicosane. Conclusion: This compound was isolated for the first time from the stem-bark of Barringtonia asiatica. The in vitro antioxidant assay of isolated compounds has shown a dose-dependent increase in free radical scavenging activity using DPPH, the antibacterial and artemia salina cytotoxicity testing showed a significant result. The chromatographic separation led to the isolation of Nerolidiol (1) and Heneicosane (2). Their structures were determined by 1H-NMR, 13CNMR, IR and MS data analysis as well as by comparison of their data with the published values.

Sign in / Sign up

Export Citation Format

Share Document