Clinical value of acute phase reactant in following the response to treatment with anti-inflammatory drugs

Functional heterogeneity in the lobule with regard to plasma protein synthesis is still debated. Therefore, we have localized in liver sections from normal rats and from rats with turpentine-induced AIR the mRNA and protein products of three genes with different alterations in their hepatic expression during an AIR: alpha 2M and alpha 1PI, two positively reacting acute-phase genes, and alpha 1I3, a negative acute-phase reactant. In normal liver, all hepatocytes expressed alpha 2M and alpha 1I3 mRNA, but a preferential expression of alpha 2M and alpha 1I3 mRNA and protein in the PP and ML zones was observed. During an AIR, the level of alpha 2M mRNA increased fourfold in the cytoplasm of PP and ML hepatocytes, while the level of cytoplasmic alpha 1I3 mRNA was decreased about fourfold in the same zones, with parallel variations in the expression of the corresponding proteins. In contrast, no significant modulation of the RNA and protein concentrations of both genes was detected in PV areas. alpha 1PI mRNA was expressed at the same levels in the three lobular zones in normal liver, but staining for the alpha 1PI protein was more intense in the PV zones. During the acute-phase response alpha 1PI mRNA levels were increased twofold in all three lobular zones, and alpha 1PI staining became homogeneous within the lobule. These results demonstrate that the location of a hepatocyte with the liver lobule can influence the expression of the three genes under study both at pre- and post-translational levels, in basal conditions, as well as during modulation of their expression during the inflammatory reaction.

Download Full-text

Sialyltransferase: A novel acute-phase reactant

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(93)90165-2 ◽

1993 ◽

Vol 105 (1) ◽

pp. 29-33 ◽

Cited By ~ 22

Author(s):

J.C. Jamieson ◽

G. McCaffrey ◽

P.G. Harder

Keyword(s):

Acute Phase ◽

Acute Phase Reactant ◽

Phase Reactant

Download Full-text

Association Between Osteoarticular Scores and Acute Phase Reactant Levels in Rheumatoid Arthritis

Journal of Medical Biochemistry ◽

10.2478/v10011-008-0031-2 ◽

2009 ◽

Vol 28 (2) ◽

pp. 116-121 ◽

Cited By ~ 1

Author(s):

Irena Kafeđiska ◽

Dejan Spasovski ◽

Todor Gruev ◽

Mane Grličkov ◽

Kočo Cakalaroski ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Acute Phase ◽

Acute Phase Reactant ◽

Control Group ◽

Time Intervals ◽

Acute Phase Reactants ◽

Mean Values ◽

Cell Counts ◽

Phase Reactant ◽

The Mean

Association Between Osteoarticular Scores and Acute Phase Reactant Levels in Rheumatoid Arthritis The aim of this prospective control study was a quantitative evaluation of the activity of rheumatoid arthritis (RA) in certain time intervals, using articular indexes (set of 28 sensitive and 28 swollen joints), laboratory parameters (Hb, Hct, Er, Le and Plt) and acute phase reactants (ESR, RF, CRP); to determine which of the acute phase reactants is the most useful biochemical marker for the evaluation of disease activity in RA; to quantify the therapeutical and laboratory differences in certain time intervals in the group with and without immunomodulatory therapy with Methotrexate. Sixty patients with RA were included, 27 of who were treated with non-steroid antiinflammatory drugs (NSAIDs) and Methotrexate (MTX). The control group consisted of 33 patients treated only with NSAIDs because of irregular controls. In the first group of patients the disease activity was estimated at four time intervals, and in the control group of patients at three time intervals following the scores of the articular indexes, blood cell counts, ESR and CRP in every patient. In the first group of patients decreased activity of RA was found upon every following control with a consecutive decrease in mean values of the scores of articular indexes with statistically significant differences at the four time intervals. Considering laboratory parameters, there were statistically significant differences in the mean values of Hb, Er, Plt, ESR, (p=0.0462, p=0.0076, p= 0.0058, p= 0.0003). Mean values of CRP did not show statistically significant differences, but the number of patients who were CRP negative increased (there were great standard deviations). In the group of patients treated only with NSAIDs, there were statistically significant differences in the mean values of the scores of articular indexes with an increse at every following control (in favour of progression of the disease). There were no statistically significant differences considering blood cell counts, ESR and CRP (in favour of permanently active disease). In conclusion, CRP is the most useful marker for the prospective follow-up of patients with RA.

Download Full-text