scholarly journals Beyaz Çürükçül Mantar Trametes versicolor’dan Elde Edilen Lakkaz Enzimi İle 2,4,6-Triklorofenol’ün Enzimatik Deklorinasyonunun Kinetik Modellemesi

2016 ◽  
Vol 4 (3) ◽  
pp. 171
Author(s):  
Arzu Ünal ◽  
Nazif Kolankaya ◽  
Abdurrahman Tanyolaç
Keyword(s):  
Kinetic Modeling ◽  
Statistical Data ◽  
White Rot Fungi ◽  
White Rot ◽  
Ms Analysis ◽  
Laccase Enzyme ◽  
Parameter Values ◽  
Kinetics Of ◽  
Substrate Model ◽  
Dual Substrate

This study examined kinetic modeling of the detoxification induced by dechlorination of laccase enzyme obtained from Trametes versicolor which is a white rot fungi strain and 2,4,6-trichlorophenol which is a toxic chlorinated phenolic compound. In the evaluation of the kinetics of detoxification induced by dechlorination of 2,4,6-trichlorophenol used as substrate, the Systat 10 software package which is a statistics program was used while two-substrate solution environment was used to explain the kinetic behavior of the dechlorination. It was observed as a result of experiments and statistical analysis that Moser equation is the optimal kinetic model. Given the statistical data obtained and the graph, the dual-substrate model was found to be compatible with the experimental data. The bio-kinetic parameter values obtained for the model that identifies enzymatic dechlorination kinetics were calculated as Vmax =9.341 ppmO2/min, Ks =38.254 g/L, Ko =20.747 ppm, R = 1.895 and N = 1.233. In the literature, no studies were found on kinetic modeling for the degradation of 2,4,6-trichlorophenol, and through this study, we have been first to conduct a study on kinetic modeling of the degradation induced by enzymatic dechlorination of 2,4,6-trichlorophenol. The GC/MS analysis results showed that the chlorophenolic compound used throughout the enzymatic dechlorination performed with laccase enzyme was degraded 80% to 100%. Based on the GC/MS analysis results, the conclusion that the enzyme responsible for the dechlorination was laccase was also found to support the findings available in literature.

scholarly journals Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
. SISWANTO ◽  
. SUHARYANTO ◽  
Rossy FITRIA
Keyword(s):  
Oil Palm ◽  
Laccase Activity ◽  
Research Result ◽  
White Rot Fungi ◽  
White Rot ◽  
Partial Purification ◽  
Empty Fruit Bunches ◽  
Optimum Ph ◽  
Laccase Enzyme

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

Kinetics of decolorization and mineralization of the azo dye Reactive Black 5 by hydrogen peroxide and UV light

2001 ◽  
Vol 44 (5) ◽  
pp. 295-301 ◽  
Author(s):  
A. Mohey El-Dein ◽  
J.A. Libra ◽  
U. Weismann
Keyword(s):  
Hydrogen Peroxide ◽  
Uv Light ◽  
Anaerobic Bacteria ◽  
White Rot Fungi ◽  
White Rot ◽  
Aerobic Biodegradation ◽  
Reactive Black 5 ◽  
Sulfate Reducing ◽  
Facultative Anaerobic Bacteria ◽  
Kinetics Of

C.I. Reactive Black 5 is one of the most used reactive dyes for textile finishing. It is a diazo dye, which can be decolorized by facultative anaerobic bacteria, sulfate reducing bacteria and aerobic white rot fungi. Mineralization by microorganisms has proven difficult. Advanced oxidation processes are promising alternatives for the decolorization and mineralization of Reactive Black 5, alone and in combination with aerobic biodegradation. The kinetics of the decolorization of Reactive Black 5 using a combination of hydrogen peroxide and UV radiation have been investigated. The rate of decolorization is first order with respect to dye concentration. It is enhanced with increasing hydrogen peroxide concentrations up to an optimum value. In our model we have correlated an empirical reaction rate expression which considers the contribution of both hydrogen peroxide and UV flux radiation based on the reaction kinetics. This empirical correlation agrees well with the experimental data for these conditions. Complete decolorization corresponded with 40-50% mineralization of the dye. Further mineralization can be achieved with extended radiation time.

scholarly journals Biotransformation of Aromatic Methyl Groups to Aldehyde Groups Using Laccase of Gloephyllum Stratum MTCC-1117

2020 ◽  
Author(s):  
RAM SAHAY
Keyword(s):  
White Rot Fungi ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
White Rot ◽  
Show Absorption Band ◽  
Yellow Colour ◽  
Sds Page ◽  
Coir Dust ◽  
Laccase Enzyme ◽  
Aldehyde Groups

Abstract Laccases has been produced by white rot fungi are involved in lignin containing natural substrates wheat-straw, bagasse, saw-dust, corn cob and coir dust particle on the production of laccase enzyme in the aqueous cultivation medium of Gloephyllum stratum MTCC1117. The approach involved concentration of aqueous filtrate by ultrafiltration and anion exchange chromatography on DEAE (diethyl aminoethyl cellulose). From SDS-PAGE analysis the molecular mass of the purified enzyme is 57 kDa. The Km and kcat values of the laccase are found to be 18 μM and 0.34 s-1 using 2,6-dimethoxyphenol as the substrate, giving a kcat/Km value is 1.70 x 103 M-1 s-1. The pH and temperature optimum were 4.5 and 40 °C respectively. The purified enzyme has yellow colour and does not show absorption band around 610 nm found in blue laccases. Moreover the conversion of methylbenzene to benzaldehyde in the lack of mediator molecules, property exhibited by yellow laccases.

Decolorization of synthetic dyes by white rot fungi, involving laccase enzyme in the process

2007 ◽  
Vol 42 (10) ◽  
pp. 1429-1435 ◽  
Author(s):  
Emrah A. Erkurt ◽  
Ali Ünyayar ◽  
Halil Kumbur
Keyword(s):  
White Rot Fungi ◽  
White Rot ◽  
Synthetic Dyes ◽  
Laccase Enzyme

scholarly journals Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
. SISWANTO ◽  
. SUHARYANTO ◽  
Rossy FITRIA
Keyword(s):  
Oil Palm ◽  
Laccase Activity ◽  
Research Result ◽  
White Rot Fungi ◽  
White Rot ◽  
Partial Purification ◽  
Empty Fruit Bunches ◽  
Optimum Ph ◽  
Laccase Enzyme

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

Laccase From White Rot Fungi Having Significant Role in Food, Pharma, and Other Industries

2018 ◽  
pp. 253-277 ◽  
Author(s):  
Ankita Kushwaha ◽  
Shweta Maurya ◽  
Ravi Kant. Pathak ◽  
Sonam Agarwal ◽  
Pankaj Kumar Chaurasia ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
White Rot Fungi ◽  
Oxidation Potential ◽  
White Rot ◽  
Textile Dye ◽  
Concomitant Reduction ◽  
Increase Productivity ◽  
Paper And Pulp ◽  
Laccase Enzyme ◽  
Quality Of Products

Laccases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase) are an interesting group of N glycosylated multicopper blue oxidase enzymes and the widely studied enzyme having a broad range of substrate specificity of both phenolic and non-phenolic compounds. They are widely found in fungi, bacteria plant, insects, and in lichen. They catalyze the oxidation of various phenolic and non-phenolic compounds, with the concomitant reduction of molecular oxygen to water. They could increase productivity, efficiency, and quality of products without a costly investment. This chapter depicts the applications of laccase enzyme from white rot fungi, having various industrial (such as textile dye bleaching, paper and pulp bleaching, food includes the baking, it also utilized in fruit juice industry to improve the quality and stabilization of some perishable products having plant oils), pharmaceutical (as it has potential for the synthesis of several useful drugs such anticancerous, antioxidants, synthesis of hormone derivatives because of their high value of oxidation potential) significance.

scholarly journals Induction of laccase in fungus, Cyathus stercoreus using some aromatic inducers

2018 ◽  
Vol 10 (1) ◽  
pp. 445-447
Author(s):  
Bhuvnesh Yadav
Keyword(s):  
Enzyme Activity ◽  
Protein Content ◽  
Ammonium Sulphate ◽  
Culture Filtrate ◽  
Plant Biomass ◽  
White Rot Fungi ◽  
White Rot ◽  
Laccase Production ◽  
Cyathus Stercoreus ◽  
Laccase Enzyme

To improve the feed quality removal of lignin from plant biomass is essential. To improve the activity of laccase of white rot fungi, aromatic inducers are used. In this study three inducers [Resorcinol (5mM and 10mM), Xylidine and Anisaldehyde] to induce the production of laccase enzyme in the culture of fungus: Cyathus stercoreus. Resorcinol (10mM) was found to be the best inducer among the rest. The enzyme activity was observed highest on the 8th day of induction (226.7U/ml). Protein content was also increased with the age of the culture. 80% ammonium sulphate was suitable for precipitating the laccase enzyme for culture filtrate. The laccase production can be enhanced with inducers and can be further used for the removal of lignin from the plant biomass.

scholarly journals Deodorization of Latex Waste and Decolorization of Textile-Coloring Agent by Omphalina sp. using Batch and Continuous Methods

2017 ◽  
Vol 3 (1) ◽  
pp. 33-42
Author(s):  
Erna Puspasari ◽  
I Made Artika ◽  
Tri Panji
Keyword(s):  
Waste Water ◽  
Industrial Waste ◽  
White Rot Fungi ◽  
White Rot ◽  
Industrial Waste Water ◽  
Continuous Methods ◽  
Dye Absorption ◽  
Laccase Enzyme ◽  
Better Than

Generally industrial waste water is a pollutant to environment as it produces strong odor and color. Omphalina sp is one of white rot fungi that can be used as an odor and color effluent reducer. Omphalina sp has laccase enzyme that plays role in deodorization and decolorization. The aim of this research was to determine the best method in deodorization and decolorization among batch and continous (pack bed flow, biotray, and rotary contactor) methods. The results of deodorization and decolorization showed that the rotary contactor method was better than the batch, pack bed flow and biotray methods. At dye concentration of 50 ppm, after 24 hours treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.520, 0.423, 0.425, and 0.357, respectively. At dye concentration of 150 ppm, after 24 hour treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.709, 0.629, 0.658, and 0.592, respectively. At dye concentration of 50 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 20.550%, 35.447%, 35.141% and 45.531% respectively. At dye concentration of 150 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 7.320%, 17.843%, 13.987%, and 22.614%. The qmaks value at dye concentration of 50 ppm after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 0.020, 0.076, 0.083, and 0.124 respectively. qmaks value at dye concentration of 150 ppm after 24 hour treatmeny for batch, pack bed flow, biotray, and rotary contactor methods was 0.009, 0.077, 0.046, and 0.100 respectively. Odor scale for batch, pack bed flow, biotray, and rotary contactor methods decreased from 5 to 2.9, 1.4, 1.6 and 1.1 respectively.

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