Cafeteria Diet Associated with the Chronic Stress Does Not Alter Biochemical and Hematological Parameters in Wistar Rats

Objective: The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.Methods: The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.Results: The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P>0.05) similar to the control group.Conclusion: The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.

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Could Co-Administration of Panax Ginseng and Vitamin E be More Effective in Reversing Nicotine- and Chronic Stress-induced Reproductive Toxicity in Male Albino Wistar Rats?

Advances in Research ◽

10.9734/air/2018/45085 ◽

2018 ◽

Vol 17 (1) ◽

pp. 1-11

Author(s):

Bisong, Sunday Agba ◽

Ukoh, Imoh Emmanuel ◽

Odey, Paul ◽

Ebong, Patrick Ekong

Keyword(s):

Vitamin E ◽

Panax Ginseng ◽

Chronic Stress ◽

Wistar Rats ◽

Reproductive Toxicity

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A 90-Day Oral Toxicity of Hydroethanolic Root Extract of Carissa spinarum in Wistar Rats

Journal of Toxicology ◽

10.1155/2021/5570206 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Komlan M. Dossou-Yovo ◽

Aboudoulatif Diallo ◽

Povi Lawson-Evi ◽

Yendubé T. Kantati ◽

Tchin Darré ◽

...

Keyword(s):

Body Weight ◽

Biochemical Parameters ◽

Wistar Rats ◽

Hematological Parameters ◽

Relative Weight ◽

Control Group ◽

Root Extract ◽

Weight Changes ◽

Oral Toxicity ◽

Significant Difference

Background. Herbal medication is a worldwide and ancient practice, mostly in developing countries, where a large part of the population is involved in this practice. Hence, studies must be conducted to evaluate their safety and efficiency to avoid or prevent toxicological risks due to their usage. In Togo, Carissa spinarum is a medicinal plant belonging to Apocynaceae family, used as an aphrodisiac or to heal some ailments including malaria, sickle cell anemia, hypertension, pain, and asthma. Notwithstanding its several ethnomedicinal benefits, just a few toxicological data associated with its chronic use are available. Objective. Therefore, this study aims to assess the toxicity of an ethanolic root extract of Carissa spinarum in Wistar rats. Methods. The 90-day oral toxicity process following OECD TG 408 guidelines is used. Male Wistar rats received Carissa spinarum root hydroethanolic extract at 500 and 1000 mg/kg for 90 days by oral gavage. Body weight changes, hematological and blood biochemical parameters, organ weight changes, malondialdehyde as a lipoperoxidation marker expressed according to tissue proteins, and histopathology of vital organs were assessed. Results. No signs of toxicity or mortality were observed during the 90 days experiment. Hematological parameters have not shown any treatment-related abnormalities. According to biochemical parameters, an increase in the chloride ion level was observed at 1000 mg/kg p < 0.01 . There was no significant difference between the treated groups and the control group concerning the malondialdehyde concentration, body weight, and organ relative weight. No changes in necropsy and histopathology of vital organs associated with extract treatment were observed. Conclusion. The results indicated that an ethanolic root extract of Carissa spinarum does not cause adverse effects, which can lead to Wistar rats’ death after 90-day oral administration at 500 and 1000 mg.

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Fatty acid utilization by young Wistar rats fed a cafeteria diet

Molecular and Cellular Biochemistry ◽

10.1007/bf00249696 ◽

1992 ◽

Vol 118 (1) ◽

pp. 67-74 ◽

Cited By ~ 7

Author(s):

M. Esteve ◽

I. Rafecas ◽

J.A. Fern�ndez-L�pez ◽

X. Remesar ◽

M. Alemany

Keyword(s):

Fatty Acid ◽

Wistar Rats ◽

Cafeteria Diet ◽

Fatty Acid Utilization

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The effect of dermal benzophenone-2 administration on immune system activity, hypothalamic-pituitary-thyroid axis activity and hematological parameters in male Wistar rats

Toxicology ◽

10.1016/j.tox.2018.04.002 ◽

2018 ◽

Vol 402-403 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Żaneta Broniowska ◽

Joanna Ślusarczyk ◽

Beata Starek-Świechowicz ◽

Ewa Trojan ◽

Bartosz Pomierny ◽

...

Keyword(s):

Immune System ◽

Wistar Rats ◽

Hematological Parameters ◽

System Activity ◽

Pituitary Thyroid Axis ◽

Male Wistar Rats ◽

Thyroid Axis

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MULTIPARTICULATE FLOATING DRUG DELIVERY SYSTEM OF ANAGLIPTIN: DESIGN AND OPTIMIZATION FOR ITS EFFICACY IN MANAGEMENT OF METABOLIC SYNDROME

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019v11i4.33249 ◽

2019 ◽

pp. 171-181

Author(s):

RAKESH V. MISHRA ◽

SHASHIKANT N. DHOLE

Keyword(s):

Metabolic Syndrome ◽

Drug Release ◽

Wistar Rats ◽

Lag Time ◽

Cafeteria Diet ◽

High Density Lipoproteins ◽

Eudragit Rs ◽

The Metabolic Syndrome ◽

Male Wistar Rats ◽

Eudragit Rl

Objective: The present research aims to design and optimize gastroretentive floating pellets of anagliptin (a dipeptidyl peptidase-4 inhibitor), so as to reduce P-Glycoprotein (PGP)–mediated efflux in the intestine hence to improve oral bioavailability. Methods: The drug-containing core pellets were prepared by extrusion and spheronization process followed by subsequent coating with three successive layers i.e. Eudragit RS 100, sodium bicarbonate (NaHCO3): hydroxypropyl methylcellulose E5LV (HPMC E5LV) and Eudragit RL 100 using fluidized bed processor. A 3 level 3 factor box-behnken design was adopted to investigate the effect of Eudragit RS 100, NaHCO3: HPMC E5LVand Eudragit RL 100 on floating lag time and drug release at 10 h. Desirability function under numerical optimization technique was used to identify the optimum formulation. Results: The study reveals the significant effect of the amount of NaHCO3 and coating level of polymers on floating lag time and drug release. The optimum system could float within 4 min and exhibited more than 85% drug release in 10 h. The pharmacokinetic study conducted in male Wistar rats indicated 2.51 fold increase in relative bioavailability of optimized formulation compare to anagliptin drug. Formulated anagliptin pellets were evaluated in cafeteria diet-induced metabolic syndrome model in male Wistar rats. Anagliptin floating pellets treatment compared to cafeteria diet group significantly inhibited increase in body weight (238.79±2.52 g vs. 277.98±3.69 g, P<0.001), calorie intake (2283.99 kcal vs. 3086.05 kcal, P<0.05) and serum levels of total cholesterol (95.19±0.61 mg/dl vs. 110.04±1.31 mg/dl, P<0.01), triglycerides (96.12±1.25 mg/dl vs. 105.99±1.29 mg/dl, P<0.01) while high-density lipoproteins levels were improved (42.15±0.92 mg/dl vs. 30.92±0.77 mg/dl, P<0.01) indicated its hypophagic and anti-hyperlipidemic effects. Conclusion: The gastroretentive floating pellets of anagliptin was obtained and could be a promising technique to deliver anagliptin with improved bioavailability in the management of the metabolic syndrome.

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Tu1866 Continuous Neonatal Chronic Stress Influences Basal Visceral Sensitivity in a Sex-Dependent Manner in Adult Wistar Rats

Gastroenterology ◽

10.1016/s0016-5085(15)33136-x ◽

2015 ◽

Vol 148 (4) ◽

pp. S-923

Author(s):

Muriel H. Larauche ◽

Mandy Biraud ◽

Jenny Molet ◽

Mulugeta Million ◽

Yvette Tache

Keyword(s):

Chronic Stress ◽

Wistar Rats ◽

Dependent Manner ◽

Visceral Sensitivity ◽

Stress Influences

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Cafeteria diet from birth to adulthood promotes hepatic steatosis and redox imbalance in Wistar rats

Nutrition & Food Science ◽

10.1108/nfs-05-2020-0191 ◽

2020 ◽

Vol ahead-of-print (ahead-of-print) ◽

Author(s):

Letícia Fernandes Gomes ◽

Sibelle Aparecida Madureira Costa ◽

Arthur Rocha-Gomes ◽

Amanda Escobar Teixeira ◽

Alexandre Alves da Silva ◽

...

Keyword(s):

Hepatic Steatosis ◽

Liver Tissue ◽

Design Methodology ◽

Wistar Rats ◽

Redox State ◽

Liver Weight ◽

Cafeteria Diet ◽

Control Group ◽

Content Type ◽

Markers Of Oxidative Stress

Purpose The purpose of this paper is to evaluate the pathological, biochemical and redox state parameters of liver tissue in Wistar rats treated from birth to adulthood (119 days) with cafeteria diet. Design/methodology/approach During the lactation, 6 liters of Wistar rats (dam + 8 pups each) were fed one of two diets: control (CTRL; n = 3) or cafeteria (CAF; n = 3) diets and water ad libitum. After weaning, the males were placed in individual cages, receiving the same diet offered to their respective dams (CTRL or CAF; n = 18) until adulthood. The following parameters were evaluated: absolute and relative liver weight; blood, liver and feces biochemistry; liver histology; and redox state of the liver. Findings When assessing the relative and absolute organ weight, no significant differences were found between the groups. The Cafeteria group exhibited higher values of serum LDL-c (p = 0.008), VLDL-c (p = 0.03) and triglycerides (p = 0.01), as well as several micro and macrovacuoles of fat accumulation, higher hepatic lipid (p = 0.03) and cholesterol (p = 0.0001) levels regarding Control group. Cafeteria group showed greater expression of glutathione-s-transferase (p = 0.03) and superoxide dismutase (p = 0.005) enzymes compared to the control group. In the case of the markers of oxidative stress, there was no difference between the groups. Originality/value A simple and standardized cafeteria diet caused an accumulation of fatty acids in liver tissue, inducing a state of hepatic steatosis besides an increased expression of antioxidant enzymes.

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