scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

scholarly journals A 90-Day Oral Toxicity of Hydroethanolic Root Extract of Carissa spinarum in Wistar Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Komlan M. Dossou-Yovo ◽  
Aboudoulatif Diallo ◽  
Povi Lawson-Evi ◽  
Yendubé T. Kantati ◽  
Tchin Darré ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Relative Weight ◽  
Control Group ◽  
Root Extract ◽  
Weight Changes ◽  
Oral Toxicity ◽  
Significant Difference

Background. Herbal medication is a worldwide and ancient practice, mostly in developing countries, where a large part of the population is involved in this practice. Hence, studies must be conducted to evaluate their safety and efficiency to avoid or prevent toxicological risks due to their usage. In Togo, Carissa spinarum is a medicinal plant belonging to Apocynaceae family, used as an aphrodisiac or to heal some ailments including malaria, sickle cell anemia, hypertension, pain, and asthma. Notwithstanding its several ethnomedicinal benefits, just a few toxicological data associated with its chronic use are available. Objective. Therefore, this study aims to assess the toxicity of an ethanolic root extract of Carissa spinarum in Wistar rats. Methods. The 90-day oral toxicity process following OECD TG 408 guidelines is used. Male Wistar rats received Carissa spinarum root hydroethanolic extract at 500 and 1000 mg/kg for 90 days by oral gavage. Body weight changes, hematological and blood biochemical parameters, organ weight changes, malondialdehyde as a lipoperoxidation marker expressed according to tissue proteins, and histopathology of vital organs were assessed. Results. No signs of toxicity or mortality were observed during the 90 days experiment. Hematological parameters have not shown any treatment-related abnormalities. According to biochemical parameters, an increase in the chloride ion level was observed at 1000 mg/kg p < 0.01 . There was no significant difference between the treated groups and the control group concerning the malondialdehyde concentration, body weight, and organ relative weight. No changes in necropsy and histopathology of vital organs associated with extract treatment were observed. Conclusion. The results indicated that an ethanolic root extract of Carissa spinarum does not cause adverse effects, which can lead to Wistar rats’ death after 90-day oral administration at 500 and 1000 mg.

scholarly journals Protective effect of blackthorn fruits (Prunus spinosa) against tartrazine toxicity development in albino Wistar rats

2019 ◽  
Author(s):  
Igori Balta ◽  
Bogdan Sevastre ◽  
Vioara Mireșan ◽  
Marian Taulescu ◽  
Camelia Raducu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Water Mixture ◽  
Water Group ◽  
Prunus Spinosa ◽  
Food Dye ◽  
Group I ◽  
Group Ii

Background: Tartrazine (Yellow 5 or E102) is a synthetic food dye able to modify perception and behavior, causingagitation, confusion, rhinitis and can produce hyperactivity syndrome in children when is combined with benzoates.Additionally, it can trigger oxidative stress which consequently generates metabolic disorders. Therefore, the studywas designed to evaluate the harmful effects of the food additive tartrazine and to observe beneficial properties ofblackthorn fruits (Prunus spinosa) on the blood and organs of albino Wistar rats.Materials and methods: This study was carried out on 20 mature Wistar rats, randomly divided into four groupsof five animals. Over the course of the experiment, the control group received only food and drinking water, group Ireceived 75 mg of tartrazine dissolved in (250 ml) water group II was given 75 mg of tartrazine and 200 mg of driedblackthorn fruit powder 200 mg dissolved simultaneously in (250 ml) of tartrazine-water mixture (aiming to reducethe tartrazine toxicity) and group III received a higher dose of tartrazine (100 mg) in (250 ml) of water.Results: At the end of the experiment, values regarding kidney and liver weight were significantly increased, whilethe weight of the spleen was slightly decreased compared with the weight of the control group. Biochemical andhematological assays, of the blood samples show that the addition of tartrazine in the diet of rats caused significantchanges in all biochemical and hematological parameters of the blood. In the group II, which received (P. spinosa)powder combined with tartrazine, the biochemical and hematological parameters had average values similar to thecontrol group.Conclusions: Histopathological assay showed that the application of tartrazine in the group I, II and III producedlesions of the kidneys, spleen and the liver for all rodents. Tartrazine was able to generate histopathological changes,which caused significantly tissue lesions of the liver and significant changes in blood parameters. Blackthorn powdershowed a promising protective role for the blood parameters but demonstrated no significant benefits for the organs.

scholarly journals Evaluation of Oxidative Stress and some Biochemical Parameters in Normal Pregnant Women

2020 ◽  
Vol 8 (1) ◽  
pp. 17-23
Author(s):  
Musher I. Kakey ◽  
Kamaran K. Abdoulrahman ◽  
Gulbahar R. Uthman
Keyword(s):  
Pregnant Women ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
First Trimester ◽  
Control Group ◽  
Sod Activity ◽  
Nonsignificant Difference ◽  
Group 2

Pregnancy is associated with many metabolic changes in normal pregnant woman, this leads to change in physiological, biochemical, and hematological parameters drastically. The test subjects were selected among those attending to Maternity Teaching Hospital in Erbil Governorate between March 2017 and August 2017. Four groups of individuals were included in this study, 230 pregnant women divided into three groups (Group 1 first trimester, Group 2 second trimester, and Group 3 third trimester) and Group 4 contained 90 nonpregnant women as control. Full automatic chemical analyzer (Cobas C311, Germany) was used to determine the biochemical parameters. The EL ×800 Absorbance Microplate Reader from BioTek (USA) instruments is used to measure the activity of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in serum. The results of this study showed a significant increase in serum albumin, glucose, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea, cholesterol, low-density lipoprotein, and MDA in pregnant women as compared to the control group, also show significant decrease in high-density lipoprotein (HDL) level, SOD activity, and CAT activity in pregnant women as compared to nonpregnant women, and nonsignificant difference in uric acid, and HDL.

The evaluation of acute and subchronic toxicities of An Phu Khang capsules in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 86-95
Author(s):  
Ha Thi Yen ◽  
Tran Thanh Tung ◽  
Dang Thi Thu Hien
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney

The purpose of this research was to evaluate the acute and subchronic toxicities of An Phu Khang capsules through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by the recommendation of WHO in Wistar rats at these doses of 0.54 g/kg b.w/day (equal to recommended human dose) and 1.62 g/kg b.w/day (3 times as high as recommended human dose) in 4 consecutive weeks. As a result, An Phu Khang capsules at the highest dose used for mice (36.29 g/kg b.w) did not show acute toxicity in mice. In terms of the subchronic toxicity test, after oral administration of An Phu Khang capsules, hematological parameters, hepato-renal functions, and microscopic images of liver and kidney at both doses were unchanged compared with the control group. In conclusion, An Phu Khang with both doses 0.54 g/kg b.w/day and 1.62 g/kg b.w/day did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals Evaluation of acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets in experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 29-38
Author(s):  
Tran Thanh Tung ◽  
Dau Thuy Duong ◽  
Pham Thi Thuy Minh ◽  
Nguyen Thu Hien ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney ◽  
Oral Doses

The study aimed to evaluate the acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets through oral administration using experimental animal models. Acute toxicity in Swiss mice was determined using the Litchfield Wilcoxon method. The subchronic toxicity in Wistar rats was evaluated according to WHO and OECD’s recommendation with oral doses of 4.68 g/kg/day (equivalent to recommended human dose) and 14.04 g/kg/day (3 times the recommended human dose) for 4 consecutive weeks. In terms of acute toxicity, “Phuong Dong Dai Trang” tablets did not express acute toxicity in mice at the highest dose used (232.14 g materials/kg). In terms of the subchronic toxicity, after oral administration of “Phuong Dong Dai Trang” tablets, hematological parameters, hepato - renal functions, and microscopic images of liver and kidney were unchanged in the treatment group compared to the control group. In conclusion, “Phuong Dong Dai Trang” tablets did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals THE EFFECTS OF P-ALAXIN ON FERTILITY AND HEMATOLOGICAL PARAMETERS IN MALE ADULT WISTAR RATS

2020 ◽  
Vol 10 ◽  
pp. 97
Author(s):  
Ifabunmi Oduyemi Osonuga ◽  
Thomas Kehinde Adenowo ◽  
Raheem Akinwunmi Akindele ◽  
Gbolahan Idowu
Keyword(s):  
Body Weight ◽  
Oral Administration ◽  
Wistar Rats ◽  
Sperm Count ◽  
Hematological Parameters ◽  
Sperm Parameters ◽  
Multi Drug Resistance ◽  
Control Group ◽  
Male Adult ◽  
Hematological Indices

P-Alaxin, is an anti-malarial drug that is highly effective in treating malaria all forms of malarial infection including multi drug resistance malaria in areas of high resistance especially in Africa. It is composed of dihydroartemisinin and piperaquine phosphate. This study investigates the effects of therapeutic dose of P-alaxin on fertility and hematological indices in rats. Fifteen male adult Wistar rats weighing between 150 and 220g were divided into three consisting of 5 rats per group. Normal saline was administered to the control group while the test and recovery groups were given 15.4mg/kg body weight of P-Alaxin orally for three days. The recovery group was allowed to recover for three days from the drug's effect. The animals were anaesthetized using diethyl ether. Blood samples were collected through cardiac puncture. Oral administration of P-alaxin (15.4mg/kg body weight) for three days reduced (p<0.05) significantly the sperm motility, sperm count, viability and testosterone as well as the PCV, Hb, WBC and RBC when compared to the control and there was no significant change in values of  pH of the tests rats' semen. Allowing the rats to recover from the effects of the drug resulted in gradual restoration of sperm parameters and hematological indices under investigation. From the study it can be safely concluded that oral administration of P-Alaxin caused reduction in the hematological and sperm parameters and serum testosterone and could reduce male Wistar rats' fertility and also lead to anemia in rats

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

scholarly journals Flaxseed, olive and fish oil influence plasmatic lipids, lymphocyte migration and morphometry of the intestinal of Wistar rats

2010 ◽  
Vol 25 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Damiana Diniz Rosa ◽  
Regiane Lopes de Sales ◽  
Luis Fernando de Sousa Moraes ◽  
Fabíola Cesário Lourenço ◽  
Clóvis Andrade Neves ◽  
...  
Keyword(s):  
Fish Oil ◽  
Intestinal Mucosa ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
Parameter Analysis ◽  
Male Rats ◽  
Histological Evaluation ◽  
Control Group ◽  
Soy Oil ◽  
Lymphocyte Migration

PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.

scholarly journals Influence of Pesticide on Biochemical and Hematological Parameters in Beninese Vegetable Farmers

2018 ◽  
Vol 9 (1) ◽  
pp. 65
Author(s):  
Lucie AYI-FANOU
Keyword(s):  
Blood Cell ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Study Group ◽  
Blood Samples ◽  
Vegetable Farmers ◽  
Insignificant Difference ◽  
Hematological And Biochemical Parameters

In several countries, a lot of researches have shown the toxicity of pesticides on farmers. Meanwhile, few of them have dealt with vegetable farmers in Benin. Therefore, this study has been carried out to assess the effects of pesticides on vegetable farmers’ health. This study is conducted on 30 vegetable farmers who used pesticides and 20 subjects as control group. Pesticides used by vegetable farmers and their risky behavior were investigated. Their blood samples were collected and some tests were performed for hematological and biochemical parameters. For the statistical analysis of the results, Student’s test was used. Our investigations revealed that vegetable farmers of Benin were exposed to different active ingredients of pesticides such as (Abamectin, Acetamiprid, Chlorpyriphos ethyl, Cyfluthrin, Cypermethrin, Emamectin benzoat, Flubendiamid, Lambdacyhalothrin, Mancozeb, Profenofos, Spinosad and Spirotétramate). The hematological parameters showed significant decrease in White blood cell (WBC), Red blood cell (RBC), Hemoglobin (HGB), Hematocrit (HTC), Mean Corpuscular Volume (MCV), Platelet (PLT) count among study group. The enzymatic activities of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) have significantly raised between vegetable farmers and the control group, while the serum concentration in urea and creatinine showed insignificant difference in the study group. These results suggest that pesticides have adverse effects on vegetable farmers of South of Benin.

scholarly journals Effect of the oral administration of monosodium glutamate during pregnancy and breast-feeding in the offspring of pregnant Wistar rats

2010 ◽  
Vol 25 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Vinicius von Diemen ◽  
Manoel Roberto Maciel Trindade
Keyword(s):  
Weight Gain ◽  
Oral Administration ◽  
Wistar Rats ◽  
Monosodium Glutamate ◽  
The Other ◽  
Control Group ◽  
Pregnant Rats ◽  
Dependent Relation ◽  
Dose Dependent

PURPOSE: Determine the effects of the MSG (monosodium glutamate) in the offspring of pregnant rats through the comparison of the weight, NAL (nasal-anal length) and IL (Index of Lee) at birth and with 21 days of life. METHODS: Pregnant Wistar rats and their offspring were divided into 3 groups: GC, G10 and G20. Each of the groups received 0%, 10% and 20% of MSG, respectively from coupling until the end of the weaning period. RESULTS: Neither weight nor NAL were different among the groups at birth. The group G20 at birth had an IL lower than the group GC (p<0,05) and with 21 days of life presented weight and NAL lower than the groups G10 and this lower than the GC (p<0,01). Otherwise the G20 at 21 days of life had the IL similar to the other two groups. The weight profit percentage from birth to the 21st day of life was lower in the G20 regarding the other two groups (p<0,01). The G20 had a NAL increase percentage from birth to the 21st day of life lower than the G10 and this lower than the GC (p<0,01). CONCLUSIONS: MSG presented a dose-dependent relation in the variables weight and NAL. It caused a decrease in the growth pattern as well as in the weight gain pattern until the 21st day of life. The IL of the group 20% had an increased in relation to the control group after 3 weeks of follow up.

Sign in / Sign up

Export Citation Format

Share Document