scholarly journals Morphological and Molecular Identification of Three Genera of the Family Heptageniidae (Ephemeroptera) from Ava Sheen Branch/ Greater Zab Tributary, North of Iraq

2020 ◽  
pp. 952-960
Author(s):  
Shelan Mustafa Khudhur ◽  
Yahya Ahmed Shekha
Keyword(s):  
Molecular Identification ◽  
Ribosomal Rna ◽  
Phylogenetic Profile ◽  
Morphological Characteristics ◽  
Nuclear Gene ◽  
16S Ribosomal Rna ◽  
Monophyletic Origin ◽  
The Family ◽  
Molecular Phylogenetic

The aquatic Heptageniidae family of Ava-sheen branch (Greater Zab Tributary) in Duhok Governorate/ Iraq was studied. Samples were collected using Surber stream sampler to study their diagnostic morphological characteristics and molecular phylogenetic profile using a nuclear gene 16s ribosomal RNA. The morphological and molecular identification supported that the three species, Epeorus longimanus, Heptagenia elegantula and Ephemerella cornutus belong to Heptageniidae family, while the molecular results also confirmed the monophyletic origin of these three genera.

Morphological and genetic description of Moniliformis necromysi sp. n. (Archiacanthocephala) from the wild rodent Necromys lasiurus (Cricetidae: Sigmondontinae) in Brazil

2020 ◽  
Vol 94 ◽  
Author(s):  
A.P.N. Gomes ◽  
N.A. Costa ◽  
R. Gentile ◽  
R.V. Vilela ◽  
A. Maldonado
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Genetic Distances ◽  
Brazilian Cerrado ◽  
Wild Rodent ◽  
The Family ◽  
Molecular Phylogenies ◽  
A New Species

Abstract A new species of Moniliformis Travassos, 1915 (Acanthocephala: Moniliformidae) is described from the hairy-tailed bolo mouse, Necromys lasiurus Lund, 1840 (Cricetidae: Sigmondontinae), captured in the Brazilian Cerrado, in Uberlândia, state of Minas Gerais, Brazil. The specimens were studied by light and scanning electron microscopy. Molecular phylogenies were inferred from partial nuclear large subunit ribosomal RNA gene sequences and partial mitochondrial cytochrome c oxidase subunit I gene. The new species is distinguished from other moniliformid species by the number of rows and number of hooks per row, size of the proboscis, size of the eggs, host species and geographical distribution. Molecular phylogenies and genetic distances analyses demonstrated that Moniliformis necromysi sp. n. forms a well-supported monophyletic group with sequences of other species of Moniliformis and is distinguished from them, which agrees with the morphological characteristics, allocating the new species to this genus and to the family Moniliformidae Van Cleave, 1924. This is the first moniliformid acanthocephalan described from a wild rodent in Brazil.

scholarly journals Molecular Identification of Bacterial Pathogen Infecting Coconut Leaf Beetle

Buletin Palma ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 147
Author(s):  
JELFINA C. ALOUW ◽  
DIANA NOVIANTI ◽  
MELDY L.A. HOSANG
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
Serratia Marcescens ◽  
Molecular Identification ◽  
Ribosomal Rna ◽  
Causal Agent ◽  
16S Ribosomal Rna ◽  
Font Size ◽  
Chain Reaction ◽  
Polymerase Chain

<p><span style="font-size: medium;">ABSTRACT </span></p><p>Many species of microorganisms can cause diseases and mortality of insect pests. Accurate detection and identification of the entomophatogens are essential for development of biological control agent to the pest. Brontispa longissima, a serious and invasive pest of coconut, was infected by bacterium causing mortality of the larvae and pupae in coconut field. Objective of the research was to identify bacterium as a causal agent of the field-infected B. longissima using molecular  technique.  Research  was  conducted  between  April  and  August 2011.  Molecular  identification  using polymerase chain reaction (PCR) amplification of 16s ribosomal RNA of the infected larvae and sequencing of the gene showed that Serratia marcescens is the causal agent of the disease.</p><p>Keywords: Brontispa longissima, coconut, 16s rRNA, Serratia marcescens.</p><p> </p><p><span style="font-size: medium;">Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama Daun Kelapa <br />Brontispa longissima(Coleoptera:Chrysomelidae)</span></p><p><span style="font-size: medium;">ABSTRAK </span></p><p>Banyak mikroorganisme dapat menimbulkan penyakit pada serangga hama.  Deteksi dan identifikasi yang akurat dari  pathogen  penyebab  penyakit  pada  serangga (entomopathogen)  hama  merupakan  tahap  yang  penting  dalam  pengembangan pengendalian biologi untuk hama tersebut.  Brontispa longissima sebagai hama penting dan bersifat  invasif pada tanaman kelapa diinfeksi oleh sejenis bakteri yang menyebabkan kematian larva dan pupa dari serangga  tersebut di lapangan. Penelitian ini bertujuan untuk mengidentifikasi organisme penyebab penyakit pada hama B. longissima dengan menggunakan teknik molekuler. Penelitian dilaksanakan pada bulan April sampai dengan Agustus  2011. Identifikasi bakteri dilakukan dengan mengamplifikasi 16s ribosomal RNA dari larva yang terinfeksi dengan menggunakan PCR (polymerase chain reaction).  Hasil analisis sekuens nukleotida 16s ribosomal RNA dari larva yang terinfeksi menunjukkan bahwa Serratia marcescens adalah bakteri penyebab dari penyakit tersebut.</p><p>Kata kunci: Brontispa longissima, kelapa, 16s rRNA, Serratia marcescens.</p>

scholarly journals Molecular Identification and Susceptibility Pattern of ClinicalNocardiaSpecies: Emergence ofNocardia crassostreaeas an Agent of Invasive Nocardiosis

2013 ◽  
Vol 24 (2) ◽  
pp. e33-e38 ◽  
Author(s):  
Saad J Taj-Aldeen ◽  
Anand Deshmukh ◽  
Sanjay Doiphode ◽  
Atqah Abdul Wahab ◽  
Mona Allangawi ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Ribosomal Rna ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
16S Ribosomal Rna ◽  
Resistant Organism ◽  
Human Pathogen ◽  
Resistant Species ◽  
Nocardia Otitidiscaviarum

BACKGROUND:Nocardiaspecies are rare, opportunistic organisms that cause disease in both immunocompetent and immunocompromised individuals.OBJECTIVE: To investigate the clinical presentations of variousNocardiainfections based on the 16S ribosomal RNA gene of the isolate, as well as related risk factors and susceptibility patterns to antimicrobial agentsMETHODS: Thirteen patients with a diagnosis of nocardiosis were included in the present study. SevenNocardiaspecies were identified by 16S ribosomal RNA. Susceptibility testing was performed using six antimicrobial agents.RESULTS: Five patients were immunocompromised, and eight were immunocompetent with predisposing factors including cystic fibrosis, tuberculosis and ophthalmic infections.Nocardiacaused pulmonary infections in eight patients (61.5%), invasive systemic infections in three patients (23%) and local (ophthalmic) infections in two patients (15.4%). In the patients with pulmonary disease, nocardiosis was caused by six species (Nocardia cyriacigeorgica,Nocardia otitidiscaviarum,Nocardia farcinica,Nocardia carnea,Nocardia testaceaandNocardia asiatica). The seventh species identified in the present study wasNocardia crassostreae.DISCUSSION:N crassostreaeis a multidrug-resistant organism that was reported to be an emerging human pathogen causing invasive nocardiosis in a patient with non-Hodgkin’s lymphoma.N farcinicawas isolated from blood in a patient with breast cancer. None of theNocardiaisolates were resistant to linezolid. OneN otitidiscaviarumisolate was a multidrug-resistant organism. All patients in the present study were treated with the appropriate antibiotics and their condition resolved without further sequelae.CONCLUSIONS: The present study is the first report onN crassostreaeas a human pathogen. The detection of multidrug-resistant species necessitate molecular identification and susceptibility testing, and should be performed for allNocardiainfections. Nocardiosis manifests various clinical features depending on theNocardiaspecies and underlying conditions.

Further polyphyly of pinnotheroid crabs: the molecular phylogenetic position of the polychaete-associated Aphanodactylidae

2018 ◽  
Vol 32 (1) ◽  
pp. 92 ◽  
Author(s):  
Ling Ming Tsang ◽  
Shane T. Ahyong ◽  
Hsi-Te Shih ◽  
Peter K. L. Ng
Keyword(s):  
Marine Invertebrates ◽  
Histone H3 ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Monophyletic Origin ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Nuclear Histone ◽  
Similar Body ◽  
High Degree

Pea crabs of the superfamily Pinnotheroidea De Haan, 1833 are known for their obligate commensal relationships with other marine invertebrates. The concomitant specialisations and adaptations of pinnotheroids have resulted in superficially similar body forms that include a high degree of structural reduction. This has confounded interpretation of their phylogenetic position in the Brachyura and interrelationships within Pinnotheroidea, though all were nevertheless believed to be united by a monophyletic origin of obligate commensalism. The family Aphanodactylidae Ahyong &amp; Ng, 2009 was proposed for a group of genera associated with tube-dwelling polychaetes formerly classified in Pinnotheridae, and provisionally retained in the Pinnotheroidea. We investigated the phylogenetic position of Aphanodactylidae using molecular data from three markers (mitochondrial 12S and 16S rRNAs, and nuclear histone H3) covering five of the 12 described aphanodactylid species and a total of 15 thoracotreme families. We found Aphanodactylidae to be monophyletic, but widely distant from Pinnotheridae and instead most closely related to Macrophthalmidae (Ocypodoidea) and Varunidae (Grapsoidea). Therefore, the family Aphanodactylidae is corroborated, but its placement in Pinnotheroidea is rejected. Instead, the phylogenetic position of Aphanodactylidae, as clearly distant from other pinnotheroids, demonstrates that obligate commensalism has evolved independently multiple times within Thoracotremata.

Trechispora yunnanensis sp. nov. (Hydnodontaceae, Basidiomycota) from China

Phytotaxa ◽  
2019 ◽  
Vol 424 (4) ◽  
pp. 253-261 ◽  
Author(s):  
TAI-MIN XU ◽  
YU-HUI CHEN ◽  
CHANG-LIN ZHAO
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Fungal Species ◽  
Monophyletic Lineage ◽  
Molecular Phylogenetic ◽  
Inference Methods

A new wood-inhabiting fungal species, Trechispora yunnanensis sp. nov., is proposed based on morphological characteristics and molecular phylogenetic analyses. The species is characterized by resupinate basidiomata, rigid and fragile up on drying, cream to pale greyish hymenial surface; a monomitic hyphal system with generative hyphae bearing clamp connections, IKI-, CB-; ellipsoid, hyaline, thick-walled, ornamented, IKI-, CB- basidiospores measuring as 7–8.5 × 5–5.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood (ML), maximum parsimony (MP) and bayesian inference methods (BPP). The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that T. yunnanensis formed a monophyletic lineage with a strong support (100% ML, 100% MP, 1.00 BPP) and was closely related to T. byssinella and T. laevis. Both morphological characteristics and results of molecular phylogenetic analyses confirmed the placement of the new species in Trechispora.

scholarly journals Quinquelaophonte enormis sp. nov., a new interstitial copepod (Harpacticoida: Laophontidae) from Korea

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10007
Author(s):  
Jaehyun Kim ◽  
Eunjung Nam ◽  
Wonchoel Lee
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
East Coast ◽  
Morphological Characteristics ◽  
Coarse Sand ◽  
Superficial Resemblance ◽  
The Family ◽  
Left And Right ◽  
Rna Genes ◽  
Swimming Leg

We collected an undescribed laophontid copepod from a coarse sand habitat on the east coast of Korea and named it Quinquelaophonte enormis sp. nov. We compared the detailed morphological characteristics of the new species with those of congeneric species. Among them, the new species shows a superficial resemblance to the Californian species Quinquelaophonte longifurcata Lang, 1965. However, the two species are easily distinguishable by the setation of the syncoxa on the maxilliped and the fourth swimming leg. The new species has the variable setation on the second to fourth swimming legs. The variations appear among individuals or between the left and right rami of a pair of legs in a single specimen. Although complex chaetotaxical polymorphism occur in this new species, we used myCOI and Cytb to confirm that the new species is not a species complex. Also, partial sequences of 18S and 28S ribosomal RNA genes were used to analyze the position of the new species within the family Laophontidae. The new speciesis the fourteenth Quinquelaophonte species in the world and the second species in Korea.

scholarly journals Phylogeny and diversity of Haploporus (Polyporaceae, Basidiomycota)

MycoKeys ◽  
2019 ◽  
Vol 54 ◽  
pp. 77-98 ◽  
Author(s):  
Meng Zhou ◽  
Li Wang ◽  
Tom W. May ◽  
Josef Vlasák ◽  
Jia-Jia Chen ◽  
...  
Keyword(s):  
New Zealand ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
New Combination ◽  
Ribosomal Rna Gene ◽  
Molecular Phylogenetic ◽  
The Usa

Four species of Haploporus, H.angustisporus, H.crassus, H.gilbertsonii and H.microsporus are described as new and H.pirongia is proposed as a new combination, based on morphological characteristics and molecular phylogenetic analyses inferred from internal transcribed spacer (ITS) and large subunit nuclear ribosomal RNA gene (nLSU) sequences. Haploporusangustisporus, H.crassus and H.microsporus occur in China, H.gilbertsonii occurs in the USA, and the distribution of H.pirongia is extended from New Zealand to Australia. Haploporusangustisporus is characterized by the distinct narrow oblong basidiospores measuring 10.5–13.5 × 3.9–5 µm. Haploporuscrassus is characterized by the presence of ventricose cystidioles occasionally with a simple septum, dissepimental hyphae usually with a simple septum, unique thick-walled basidia and distinctly wide oblong basidiospores measuring 13.5–16.5 × 7.5–9.5 µm. Haploporusgilbertsonii is characterized by its large pores (2–3 per mm), a dimitic hyphal structure with non-dextrinoid skeletal hyphae and wide oblong basidiospores measuring 12–15 × 6–8 µm. Haploporusmicrosporus is characterized by distinctly small pores (7–9 per mm), the presence of dendrohyphidia, and distinctly small ellipsoid basidiospores measuring 5.3–6.7 × 3–4.1 µm. Haploporuspirongia is proposed as a new combination. Haploporusamarus is shown to be a synonym of H.odorus and Pachykytosporawasseri is considered a synonym of H.subtrameteus.

scholarly journals The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae

1991 ◽  
Vol 137 (5) ◽  
pp. 1215-1222 ◽  
Author(s):  
N. K Fry ◽  
S. Warwick ◽  
N. A. Saunders ◽  
T. M. Embley
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
The Family

scholarly journals Multigene Phylogeny Reveals Haploanthostomella elaeidis gen. et sp. nov. and Familial Replacement of Endocalyx (Xylariales, Sordariomycetes, Ascomycota)

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 486
Author(s):  
Sirinapa Konta ◽  
Kevin D. Hyde ◽  
Prapassorn D. Eungwanichayapant ◽  
Samantha C. Karunarathna ◽  
Milan C. Samarakoon ◽  
...  
Keyword(s):  
New Genus ◽  
Elaeis Guineensis ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Multigene Phylogeny ◽  
The Family ◽  
Palm Fungi ◽  
Molecular Phylogenetic ◽  
Metroxylon Sagu ◽  
A New Species

During our investigation of palm fungi in Thailand, two interesting taxa from Elaeis guineensis and Metroxylon sagu (Arecaceae) were collected. Based on phylogenetic analyses of a combined dataset of ITS, LSU, rpb2, and tub2 nucleotide sequences as well as unique morphological characteristics, we introduce the new genus Haploanthostomella within Xylariales, and a new species Endocalyx metroxyli. Additionally, in our study, the genus Endocalyx is transferred to the family Cainiaceae based on its brown conidia and molecular phylogenetic evidence.

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