scholarly journals Determination of some virulence factors of Citrobacter freundii isolated from Iraqi patients

2021 ◽  
pp. 3358-3365
Author(s):  
Asmaa Easa Mahmood ◽  
Amidah Ali Atyah
Keyword(s):  
Urinary Tract Infection ◽  
Virulence Factors ◽  
Susceptibility Testing ◽  
Citrobacter Freundii ◽  
Beta Lactamase ◽  
Bacterial Identification ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Extended Spectrum Beta Lactamase

      This study included the isolation and identification of Citrobacter freundii from 220 samples collected from inpatients and outpatients suffering from urinary tract infection (UTI) and identified at the laboratory of the General Samarra Hospital in Samarra City, Iraq. The study was conducted to investigate some of the virulence factors produced by C. freundii. The results showed that 67 isolates were  belonging to the C. freundii, with a rate of  30.45%. Twenty eight samples were from inpatients (41.8%)  and 39 samples were from outpatients. The bacterial identification was based on cultural and biochemical tests and confirmed by using VITEK2 compact system. Virulence factor results showed that all isolates were not blood hydrolyzing whereas they produced protease. Seven isolates (10.4%) produced biofilm, five from inpatients and two from outpatients,  at rates of 17.8%  and 5.1%, respectively. The results showed that 17 (25.4%) of the pathogenic isolates were β-lactamase producers, as determined by the iodometric  method, twelve of them (17.9%) were from inpatients and 5 (7.5%) from outpatients.  Four isolates of C. freundii produced Extended Spectrum Beta-lactamase (ESβL) enzymes, three from inpatients and one from outpatients, with ratios of 4.5% and 1.4%, respectively. Also, the via B gene, which is responsible for virulence factors, was investigated using PCR. The results showed that 12 isolates from inpatients and 4 isolates from outpatients were harboring this gene. The antimicrobial susceptibility testing by Kirby-Bauer’s method showed that all isolates that produced β-lactamase were resistant to antibiotics.

scholarly journals YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI

2017 ◽  
pp. 54-58 ◽  
Author(s):  
M. A. Makarova ◽  
L. V. Suzhaeva ◽  
L. A. Kaftyreva
Keyword(s):  
Virulence Factors ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Gene Coding ◽  
Pcr Method ◽  
Diarrhea Syndrome

Aim. Study the prevalence of diarrhea-genic E. coli of the enteroaggregative group in children with intestine dysbiosis. Materials and methods. PCR method was used to study virulence factors in 511 strains of E. coli isolated during bacteriologic study of feces samples from 393 children aged less than 2 years. Sensitivity to antibiotics was determined by disc-diffusion method, results interpretation - according to clinical recommendations Determination of sensitivity of microorganisms to antimicrobial preparations, 2015. Results. 23 enteroaggregative E. coli strains were identified (EAggEC). All the strains had aaf gene coding aggregative-adhesion fimbriae and 4 other genes (aggR, ast, aap, aatA) in various combinations coding virulence factors EAggEC. 19 strains (87%) were not sensitive to antimicrobial preparations. Resistance to extended spectrum cephalosporins was determined by the production of extended spectrum beta-lactamase (ESBL) of CTX-M genetic family and AmpC cephalosporinase. Conclusion. Results of the study have shown that 6% of children with intestine dysbiosis are EAggEC carriers, that gives evidence on the necessity of detection of EAggEC strains - a novel group of diarrhea-genic E. coli not only in patients with diarrhea syndrome, but also using intestine dysbiosis.

scholarly journals Isolation, identification and antibiogram profile of Aeromonas hydrophila from broiler chickens in Mymensingh Sadar, Bangladesh

2020 ◽  
Vol 4 (1) ◽  
pp. 22-30
Author(s):  
Basana Sarker ◽  
Mohammad Arif ◽  
Nilofa Eashmen ◽  
Mir Rowshan Akter ◽  
SM Lutful Kabir
Keyword(s):  
Aeromonas Hydrophila ◽  
Broiler Chickens ◽  
Susceptibility Testing ◽  
Specific Detection ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Alkaline Peptone Water ◽  
Sensitivity Pattern ◽  
Peptone Water

Investigation of Aeromonas hydrophila was conducted to assess the microbial quality of broiler chickens from July to November 2019. A total of 60 samples from 20 broiler chickens were collected from two different locations of Mymensingh Sadar: KR market, Bangladesh Agricultural University (BAU) and Shesh mor bazar (10 birds from each location). Samples included 20 skins, 20 legs and 20 breast samples from 20 broiler chickens. PCR was done for the specific detection of each isolate and finally antimicrobial susceptibility testing was performed to check sensitivity pattern of each isolate. Alkaline peptone water was used for processing and enrichment of the samples followed by inoculation onto Aeromonas selective agar supplemented with ampicillin for the isolation and identification of A. hydrophila. Out of these 60 samples, 27 isolates were confirmed as A. hydrophila through biochemical tests and PCR where 55.56% isolates were recovered from Shesh mor market and other 44.4% isolates from KR market, BAU. Source-wise analysis revealed that maximum isolates of A. hydrophila were recovered from skin (59.26 %) followed by leg (22.22 %) and breast samples (18.52 %). PCR test revealed that all 27 isolates were found carrying lip gene which is specific for A. hydrophila. Isolates of A. hydrophila were found sensitive to ciprofloxacin (92%), gentamycin (66%) and chloramphenicol (50%); intermediate against erythromycin (50%), tetracycline (50%) and imipenem (50%); resistant against co-trimoxazole (84%) and ampicillin (100%). From the present study, it was found that samples were considerably contaminated with Aeromonas hydrophila causing risks for public health. Necessary control actions should be taken in every steps of production, processing and marketing for mitigation of this contamination. Asian Australas. J. Food Saf. Secur. 2020, 4 (1), 22-30

Isolation and Identification of Fungal Isolates caused Otomycosis from some Hospitals and Clinics in Mosul with Determination of Their Virulence Factors

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Nabeel Al-Sharrad ◽  
Muhammad A. Al-Kataan ◽  
Maha A. Al-Rejaboo
Keyword(s):  
Fungal Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Fungal Pathogens ◽  
Candida Parapsilosis ◽  
Isolation And Identification ◽  
Fungal Isolates ◽  
Biofilm Production ◽  
Etiological Agents

Otomycosis is a fungal infection that frequently involves the external auditory canal. In this study, we aimed to isolation and identification the fungal isolates as etiological agents of otomycosis from some hospitals and clinics in Mosul with determination of their virulence factors of fungal etiological agents. Positive fungal infection was found in (43) samples (71.6%). The most common fungal pathogens were Candida and Aspergillus species, with Candida parapsilosis being the predominant isolates in (11) samples (16.6%). Otomycosis was more common in Female in (26) samples (43.3%).Otomycosis was the highest prevalence aged group 15-40 years (19) samples (31.3%). The present study of virulence factors revealed that the highest biofilm formation isolates were C. parapsilosis is (10) isolates which were distributed between (2) strong and (8) weak biofilm formation.Where C.trpicales, was recorded as least isolates for biofilm production.

scholarly journals Antibiotics Profile, Prevalence of Extended-Spectrum Beta-Lactamase (ESBL), and Multidrug-Resistant Enterobacteriaceae from Different Clinical Samples in Khartoum State, Sudan

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Ehssan H. Moglad
Keyword(s):  
Tracheal Aspirate ◽  
Multidrug Resistant ◽  
Vaginal Swab ◽  
Clinical Samples ◽  
Beta Lactamase ◽  
Biochemical Tests ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Different Sources

One of the global requirements for controlling the occurrence of resistance to antimicrobial drugs is to understanding the resistivity profile of various clinical isolates. Therefore, this study aimed to deliver the indication of different resistant profiles of clinically isolated Enterobacteriaceae from different sources of samples from Khartoum state, Sudan, and to determine the prevalence rate of extended-spectrum beta-lactamase (ESBL), multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrug-resistant (PDR) bacteria. A total of 144 Gram-negative bacteria were collected from different sources (vaginal swab, urine, catheter tip, sputum, blood, tracheal aspirate, pus, stool, pleural fluid, and throat swab). Samples were subcultured and identified according to their cultural characteristics and biochemical tests. Antimicrobial susceptibility test was performed for twenty-four antibiotics from eleven categories against all isolated Enterobacteriaceae according to the recommendation of Clinical and Laboratory Standards Institute (CLSI). The result showed that out of 144 isolates, Escherichia coli and Klebsiella pneumoniae were predominant isolates with the percentage of 47.9 and 25%, respectively. The prevalence of ESBL was higher in K. pneumonia (38.9%) than E. coli (34.8%). All isolated E. coli were sensitive to nitrofurantoin and tigecycline. There was a high prevalence of MDR Enterobacteriaceae, and only one isolate was XDR, while PDR was zero for all isolated bacteria. Active antimicrobial-resistant (AMR) observation through constant data sharing and management of all stakeholders is crucial to recognize and control the AMR global burden. Also, effective antibiotic stewardship procedures would be applied to limit the unreasonable expenditure of antibiotics in Sudan.

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