scholarly journals Paku Kawat Lycopodiella cernua (L.) Pic. Serm. (Lycopdiaceae-Lycopodiales) dari Provinsi Riau – Kajian Morfologi dan Sekuen DNA berdasarkan Primer RBCL

2019 ◽  
Vol 7 (1) ◽  
pp. 43
Author(s):  
Nery Sofiyanti ◽  
Mayta Novaliza Isda
Keyword(s):  
Dna Sequence ◽  
Morphological Characters ◽  
Pcr Method ◽  
Blast Result

Moss club (Lycopodiella cernua) is one of fern members distributed in Riau Province. The aimed of this study were to characterized the morphological characters and to analized its DNA sequence based on rbcl primer. Samples were collected from the field, documented and characterized the morphological charaters. DNA were isolated and amplified using PCR method and rbcl primer.  DNA sequnces were then analized using BLAST   Lycopodiella cernua collected from Riau Province was characterized by having creeping and erect rhizome that covered by microphyl, and terminal strobili. Rbcl fragmen of Lycopodiella cernua was sucssesfully amplified with 643 bp length. BLAST result confirmed the  rbcl fragmen of this club moss.

scholarly journals SEQUENCE ANALYSIS OF 18s DNA OF Melosira sp., Dunaliella sp., Isochrysis sp. AND Porphyridium sp.

2015 ◽  
Vol 2 (1) ◽  
pp. 592
Author(s):  
Lucia Kusumawati ◽  
Ruben Wahyudi ◽  
Reinhard Pinontoan ◽  
Maria Gorreti Lily Panggabean
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Morphological Characters ◽  
Rdna Sequences ◽  
Pcr Products ◽  
18S Rdna Sequences ◽  
Pcr Method ◽  
High Level

<p>Phytoplankton has high level of biodiversity. In previous years phytoplankton was identified by their morphological characters. However, their morphology might change in different environments. These difficulties can be overcome by comparing their 18S rDNA sequences. This research is aimed to verify the identity of Melosira sp., Dunaliella sp., Isochrysis sp. and Porphyridium sp. Here, PCR method was used to amplify 18s DNA sequences. Three primer pairs were used, i.e. 18S-F and 18S-R; 501F and 1700R; 18S-2F and 18S-2R. PCR products were sequenced. MEGA5 was used to make phylogenetic tree. Genus verification for Isochrysis sp., Dunaliella sp. and Melosira sp. were conducted successfully using Blast and phylogenetic tree. 18s DNA sequence of Porphyridium sp. shows an interesting result and needs further verification.</p><p><br /><strong>Keywords</strong>: Phytoplankton, Melosira sp., Dunaliella sp., Isochrysis sp., Porphyridium sp.</p>

Two new species of the genus Flavoperla (Plecoptera: Perlidae) from Guangxi, China

Zootaxa ◽  
2021 ◽  
Vol 5032 (2) ◽  
pp. 247-261
Author(s):  
RAORAO MO ◽  
JINJUN CAO ◽  
GUOQUAN WANG ◽  
WEIHAI LI ◽  
DÁVID MURÁNYI
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Southern China ◽  
Morphological Characters ◽  
Sequence Comparisons ◽  
Autonomous Region ◽  
Guangxi Zhuang Autonomous Region ◽  
Taxonomic Relationships ◽  
Two New Species

Two new species, Flavoperla retusata Mo, Li & Wang, sp. nov. and F. yangi Mo, Li & Murányi, sp. nov. are proposed from the Guangxi Zhuang Autonomous Region of southern China. Distinctness of the new species is based on morphological characters and DNA sequence comparisons with their closest known relative, F. galerispina Mo, Wang & Li, 2020. The taxonomic relationships of the two new species and related congeners are discussed.  

First report of Hericium cirrhatum from Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
First Time

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.

Genetic divergence and geographic diversification in Nautilus

Paleobiology ◽  
1995 ◽  
Vol 21 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Charles G. Wray ◽  
Neil H. Landman ◽  
W. Bruce Saunders ◽  
James Bonacum
Keyword(s):  
Dna Sequence ◽  
Phylogenetic Relationships ◽  
Nuclear Dna ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Distinct Species ◽  
Morphological Characters ◽  
Geographic Diversification ◽  
Isolated Populations ◽  
Systematic Analysis

Despite exhaustive investigation of present-day Nautilus, the phylogenetic relationships of the five or six recognized species within this genus remain unclear. Mitochondrial and nuclear DNA sequence data plus a suite of morphological characters are used to investigate phylogenetic relationships. Systematic analysis of the morphological variation fails to characterize described species as independent lineages. However, DNA sequence analysis indicates that there are three geographically distinct clades consisting of western Pacific, eastern Australian/Papua-New Guinean, and western Australian/Indonesian forms. The morphologically and genetically distinct species Nautilus scrobiculatus falls outside the three geographically recognized assemblages. Members of the genus Nautilus also exhibit low levels of sequence divergence. All these data suggest that Nautilus is currently undergoing diversification, which may have begun only several million years ago. These data also suggest that some of the morphological features used to define Nautilus species may simply represent fixed variations in isolated populations within the same species.

Perssoniella and the genera of Schistochilaceae: a new classification based on molecular phylogenies

2010 ◽  
Vol 23 (4) ◽  
pp. 229 ◽  
Author(s):  
Xiaolan He ◽  
David Glenny
Keyword(s):  
Dna Sequence ◽  
New Caledonia ◽  
Sequence Data ◽  
Strong Support ◽  
Morphological Characters ◽  
Monotypic Genus ◽  
New Classification ◽  
Dna Sequence Data ◽  
The Family ◽  
Molecular Phylogenies

The monotypic genus Perssoniella with P. vitreocincta Herzog, endemic to New Caledonia, possesses a series of unique morphological characters and it has been assumed that the genus, assigned to the family Perssoniellaceae and suborder Perssoniellineae, is very isolated but sister to the family Schistochilaceae. The systematic identity of Perssoniella vitreocincta was studied using DNA sequence data for the chloroplast rbcL, rps4 and trnL-F regions. Our analyses placed Perssoniella vitreocincta within the family Schistochilaceae, and within Schistochila itself, with strong support. It suggests that retaining Perssoniella as an independent genus is untenable and we transfer it to the genus Schistochila. Our results indicate that Perssoniella vitreocincta is not an archaic species, as presupposed earlier. The differentiating characters in Perssoniella are mostly probably later derived, rather than ancestral. Our analyses also placed Pachyschistochila and Paraschistochila within Schistochila, again with strong support. We also transfer these two genera to Schistochila.

Taxonomic revision of Bromeliaceae subfam. Tillandsioideae based on a multi-locus DNA sequence phylogeny and morphology

Phytotaxa ◽  
2016 ◽  
Vol 279 (1) ◽  
pp. 1 ◽  
Author(s):  
MICHAEL H.J. BARFUSS ◽  
WALTER TILL ◽  
ELTON M.C. LEME ◽  
JUAN P. PINZÓN ◽  
JOSÉ M. MANZANARES ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Nuclear Dna ◽  
Taxonomic Revision ◽  
Plastid Dna ◽  
Morphological Characters ◽  
Seed Morphology ◽  
Identification Key ◽  
New Genera ◽  
Stigma Morphology

A taxonomic revision of Bromeliaceae subfam. Tillandsioideae is presented based on a multi-locus DNA sequence phylogeny (viz., plastid DNA loci rpoB-trnC-petN, trnK-matK-trnK, and ycf1, and the nuclear DNA gene PHYC) and new or re-evaluated morphology (e.g., leaf, inflorescence, sepal, petal, ovary, stigma, stamen, pollen, ovule, and seed morphology). This enables the circumscription of monophyletic units using synapomorphic combinations of diagnostic morphological characters. Stigma morphology has proven to be indicative for super-specific taxa in Tillandsioideae. One new stigma type and several subtypes of previously described stigmas were found. The four tribes proposed earlier are mostly confirmed, but Catopsideae replaces the formerly used name Pogospermeae for the monotypic tribe of Catopsis. In addition, the two new subtribes Cipuropsidinae and Vrieseinae are proposed within tribe Vrieseeae. Several new genera are established to render taxonomic units monophyletic and morphologically well circumscribed. They represent segregates of either Mezobromelia (Gregbrownia: 4 spp.), Tillandsia (viz., Barfussia: 3 spp., Josemania: 5 spp., Lemeltonia: 7 spp., Pseudalcantarea: 3 spp., and Wallisia: 4 spp. and 1 hybrid), or Vriesea (viz., Goudaea: 2 spp., Jagrantia: 1 sp., Lutheria: 4 spp., Stigmatodon: 18 spp., and Zizkaea: 1 spp.). The new subgenera Tillandsia subg. Pseudovriesea and T. subg. Viridantha are established, and T. subg. Aerobia is resurrected. An identification key to all accepted genera of Bromeliaceae subfam. Tillandsioideae is provided. Furthermore, to clarify nomenclatural uncertainties, typifications are proposed for Catopsis subg. Tridynandra, Thecophyllum [unranked] Biflorae, Tillandsia subg. Aerobia, T. sect. Caricifoliae, T. sect. Conostachys, T. sect. Cyathophora, T. sect. Eriophyllum, T. sect. Macrocyathus, T. sect. Platystachys Baker auct. non al., Tillandsia sect. Strepsia, Vriesea subg. Conostachys Mez auct. non al., T. lindenii K. Koch auct. non al., and T. macropetala.

scholarly journals Three new species of Dicephalospora from China as revealed by morphological and molecular evidences

MycoKeys ◽  
2019 ◽  
Vol 55 ◽  
pp. 87-99
Author(s):  
Huan-Di Zheng ◽  
Wen-Ying Zhuang
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Maximum Parsimony ◽  
Morphological Characters ◽  
Neighbor Joining ◽  
Sequence Analyses ◽  
Three New Species ◽  
Yellowish White

Three new species of Dicephalospora are introduced based on morphological characters and DNA sequence analyses (maximum parsimony and neighbor-joining methods), viz. D.albolutea, D.shennongjiana, and D.yunnanica. All of them lack mucilaginous caps at ascospore poles. Dicephalosporaalbolutea is distinguished by cream to yellowish white apothecia and slightly curved ascospores. Dicephalosporashennongjiana is characterized by yellow apothecia, elliptical-fusoid ascospores 19−22 × 7−8.8 μm, and J+ asci 130−150 × 14−16.5 μm. Dicephalosporayunnanica is distinguished by orange apothecia and fusoid ascospores 16.5−25.3 × 3.3−3.5 μm. Descriptions and illustrations of the new species as well as a key to the known species in the genus are provided.

scholarly journals DISTRIBUTION OF TROPICAL EEL GENUS Anguilla IN INDONESIA WATER BASED ON SEMI-MULTIPLEX PCR

2012 ◽  
Vol 7 (2) ◽  
pp. 139 ◽  
Author(s):  
Melta Rini Fahmi ◽  
Laurent Pouyaud ◽  
Patrick Berrebi
Keyword(s):  
Morphological Characters ◽  
Wide Distribution ◽  
Sample Collection ◽  
Fish Size ◽  
Distribution Ranges ◽  
Pcr Method ◽  
Glass Eels ◽  
Very High ◽  
Tropical Eel ◽  
Tropical Eels

Tropical eels living in Indonesian waters are known to be composed of several species, but their real listing together with their distribution ranges need to be established. The main difficulties are the very high number of islands with perennial rivers where these species are living during the growth phase of their life cycle. It is difficult, sometimes impossible, to determine the species using morphological characters, moreover on glass eels. In order to establish the geographic distribution of tropical eels of the genus Anguilla in Indonesian waters, a total 1,115 specimens were collected between 2008 and 2012. Sample collection was done in the growth habitats that are rivers and estuaries by commercial nets of different categories according to the fish size. All samples were identified genetically using the recently developed semimultiplex PCR method. We recognized four species and subspecies with wide distribution: Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla marmorata, and Anguilla interioris; two species with limited distribution, close to endemism: Anguilla celebesensis and Anguilla borneensis and one subspecies Anguilla nebulosa nebulosa that is only spread in river flowing into Indian Ocean.

scholarly journals Toward a Natural Classification of Botryosphaeriaceae: A Study of the Type Specimens of Botryosphaeria sensu lato

2021 ◽  
Vol 12 ◽  
Author(s):  
Ying Zhang ◽  
Yupei Zhou ◽  
Wei Sun ◽  
Lili Zhao ◽  
D. Pavlic-Zupanc ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Phylogenetic Position ◽  
Type Specimens ◽  
Natural Classification ◽  
Dna Sequence Data

The genus Botryosphaeria includes more than 200 epithets, but only the type species, Botryosphaeria dothidea and a dozen or more other species have been identified based on DNA sequence data. The taxonomic status of the other species remains unconfirmed because they lack either morphological information or DNA sequence data. In this study, types or authentic specimens of 16 “Botryosphaeria” species are reassessed to clarify their identity and phylogenetic position. nuDNA sequences of four regions, ITS, LSU, tef1-α and tub2, are analyzed and considered in combination with morphological characteristics. Based on the multigene phylogeny and morphological characters, Botryosphaeria cruenta and Botryosphaeria hamamelidis are transferred to Neofusicoccum. The generic status of Botryosphaeria aterrima and Botryosphaeria mirabile is confirmed in Botryosphaeria. Botryosphaeria berengeriana var. weigeliae and B. berengeriana var. acerina are treated synonyms of B. dothidea. Botryosphaeria mucosa is transferred to Neodeightonia as Neodeightonia mucosa, and Botryosphaeria ferruginea to Nothophoma as Nothophoma ferruginea. Botryosphaeria foliicola is reduced to synonymy with Phyllachorella micheliae. Botryosphaeria abuensis, Botryosphaeria aesculi, Botryosphaeria dasylirii, and Botryosphaeria wisteriae are tentatively kept in Botryosphaeria sensu stricto until further phylogenetic analysis is carried out on verified specimens. The ordinal status of Botryosphaeria apocyni, Botryosphaeria gaubae, and Botryosphaeria smilacinina cannot be determined, and tentatively accommodate these species in Dothideomycetes incertae sedis. The study demonstrates the significance of a polyphasic approach in characterizing type specimens, including the importance of using of DNA sequence data.

scholarly journals Systematics of Scleranthus (Caryophyllaceae)

2021 ◽  
Author(s):  
◽  
Robin David Smissen
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Sequence Divergence ◽  
Morphological Characters ◽  
Dna Sequence Analysis ◽  
Its Sequences ◽  
Data Sets ◽  
Nuclear Its ◽  
Floral Characters

<p>Scleranthus is a genus of about 12 species of herbaceous flowering plants or small shrubs with a disjunct Eurasian/Australasian distribution. Monophyly of the genus is supported by the close similarity of gynoecial development of all species and consistent with nuclear ITS DNA sequence analysis. Traditionally the genus had been divided into two sections, section Scleranthus and section Mniarum. Section Mniarum is exclusively Australasian while section Scleranthus has been circumscribed to contain exclusively European species or a combination of European and Australasian species. Pollen and floral characters align the species into Australasian and Eurasian groups also supported by nuclear ITS DNA sequence analysis. Section Scleranthus as more broadly defined (i.e., sensu West and Garnock-Jones, 1986) is therefore at least paraphyletic or at worst polypyhyletic. Phylogenetic reconstructions based on morphological characters differ from those based on ITS sequences in supporting different relationships within the Australasian species of Scleranthus. Hybridisation and introgression within the genus are discussed and suggested as the cause of discordance between morphology and DNA sequence based trees. Low sequence divergence among Scleranthus ITS sequences suggests that the European and Australasian clades within the genus diverged within the last l0 million years. Biogeographic implications of these dating and competing hypotheses explaining the disjunct North-South distribution of the genus are discussed. Nuclear ITS and chloroplast ndhF DNA sequences both suggest that Scleranthus belongs to a clade within the family Caryophyllaceae consisting of members of subfamilies Alsinoideae and Caryophylloideae. Phylogenetic relationships between genera belonging to the three subfamilies of Caryophyllaceae (Alsinoideae, Caryophyloideae, and Paronychioideae) are addressed in this thesis through ndhF sequence analysis, which provides no support for the monophyly of traditionally recognised groups. Morphological character data sets are likely to always encompass multiple incongruent data partitions (sensu Bull et al. 1993). It may therefore be appropriate to combine data from DNA sequence and morphology for parsimony analysis even where the two are significantly incongruent.</p>

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