scholarly journals Oleuropein levels of Anatolian olive leaves and correlated antioxidant, antidiabetic, and anti-inflammatory activities

2020 ◽  
Vol 14 (2) ◽  
pp. 133-141
Author(s):  
Zeynep Kalaycıoğlu ◽  
Merve Kopar ◽  
F. Bedia Erim
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Strong Correlations ◽  
Olive Leaf ◽  
Olive Leaves ◽  
Leaf Extracts ◽  
Electrophoretic Method ◽  
Optimal Separation ◽  
Anti Inflammatory

Oleuropein, the major active compound in olive leaf, has been of considerable interest for its many health benefits. This work aims the determination of oleuropein in olive leaves from Turkey by a rapid and simple capillary electrophoretic method, and then to correlate the oleuropein amounts and the bioactivities of olive leaf extracts. The optimal separation medium was composed of 30 mmol/L borate (pH: 9.6), 25 mmol/L2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD), 10% (v/v) methanol. Moreover, olive leaf extracts were examined for antioxidant, antidiabetic, and anti-inflammatory activities. The antioxidant activities were determined by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. The antidiabetic activities were predicted using α-glucosidase inhibitory effects. For the anti-inflammatory activities of the extracts, their reduction power of pro-inflammatory tumor necrosis factor (TNF)-alpha were measured. Oleuropein concentration ranged between 11.7-106 mg/g dry leaf. Strong correlations were detected between each biological activity and the oleuropein content of olive leaves

scholarly journals Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

2020 ◽  
Vol 12 (2) ◽  
pp. 177-183
Author(s):  
Idorenyin Nwaehujor ◽  
Samuel Akande ◽  
Olubunmi Atolani ◽  
Gabriel Olatunji
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Membrane ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Proteinase Inhibition

Inflammation has stimulated significant worldwide scientific interest because of its implication in many human diseases. Most inflammations are caused by reactive oxygen species or free radicals. Annona muricataleaf extracts were investigated for their in-vitroantioxidant and anti-inflammatory potentials. Annona muricataleavesweredried at room temperature, blended using a mill.and extracted with solvents of varying degree of polarities. The solventsused were hexane, ethyl acetate,and ethanol. After sequential extraction, the crude extracts were examined for their in-vitroanti-inflammatory activities on lipoxygenase inhibition, proteinase inhibition, albumin denaturation inhibition,and red blood cell membrane stabilization assays,while the antioxidant activities were examined using DPPH, ABTS and hydrogen peroxide assays. The results showed that the ethanol extract had significantlyhigher albumin denaturation inhibition activity at 500 μg/mL (p < 0.01). The activity of all the extracts on proteinase inhibition decreased with the increase in concentration of the extracts. Indomethacin (standard), ethanol extract,and ethyl acetate extract exhibited a dose dependent increase in lipoxygenase activity. The ethanol extract showed highred blood cell membrane stabilization activity at 500 μg/mL and the activity was comparable with that of the standard (diclofenac). Hydrogen peroxide scavenging activity of the extracts and standard (Vitamin C) were comparable at 20 –100 μg/mL. The ethanol extract showed significantly higher(p < 0.01) DPPH radical scavenging activity compared with other extracts. A similar trend was also observed for ABTS radical scavenging activity. Generally,the ethanol extract exhibited higher anti-inflammatory and antioxidant activities in most of the assays, this could be attributed to the polar compounds present in the extract.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5295
Author(s):  
Xinyu Zhao ◽  
Ruyi Chen ◽  
Yueyue Shi ◽  
Xiaoxi Zhang ◽  
Chongmei Tian ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preparative Hplc ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

This study aimed to isolate, prepare and identify the main flavonoids from a standardized Smilax glabra flavonoids extract (SGF) using preparative HPLC, MS, 1H NMR and 13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+ radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p < 0.01) and the protein expression of NF-κB p-p65 (p < 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids in S. glabra.

scholarly journals Antioxidant, Anti-Inflammatory and Wound Healing of Biosynthetic Gold Nanoparticles Using Mangosteen (G. Mangostona)

2021 ◽  
Vol 8 (2) ◽  
pp. 59-74
Author(s):  
Abdulkadir Mohammed Noori Jassim ◽  
Gufran Mohammed Shafy ◽  
Mustafa Taha Mohammed ◽  
Safana Ahmed Farhan ◽  
Omar Mohammed Noori
Keyword(s):  
Gold Nanoparticles ◽  
Wound Healing ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Spectroscopic Method ◽  
Radical Scavenging ◽  
New Drugs ◽  
Garcinia Mangostana ◽  
Anti Inflammatory ◽  
Peel Extract

In current research, the synthesis of gold nanoparticles was achieved via reducing of gold ions in aqueous solution with Garcinia mangostana (G. mangostana) peel extract. The optimum concentration of gold (Au) solution, concentration ratio of Au solution and extract, temperature, time and pH, the synthesized AuNPs (G. mangostana-gold nanoparticles) were studied by using UV-Vis, FT-IR, AAS, AFM, SEM and Zitasizer. The absorbance peak is noticed between 535-550 nm via UV-Vis spectroscopic method. The SEM, AFM analysis were proofed the particle as spherical in structure and their size between 15-100nm. Therefore, mechanism of AuNPs synthesis had been suggested. Also, the antibacterial activity was examined using different bacteria as well as free radical scavenging activity was tested using 1, 1-Diphenyl-2-picrylhydrazyl (DPPH). The AuNPs produced through biosynthesized method indicated a much elevated antioxidant activity as compared to peel extract of G. mangostana. Toxicity of the NPs and extract were tested via giving orally dose 50 mg/b.w. to mice. Diagnosis of the data (pathological changes) indicated that the AuNPs was non-toxic. The G. mangostana peel extract and AuNPs synthesized by this extract were converted to a cream and used as a wound healing cream. As a results, the AuNPs exhibited important role in wound healing progression compared to control, which may be attributed to their anti-inflammatory, antibacterial and antioxidant activities. Therefore, this research confirms its important use of AuNPs and can be utilized as promising agents for in the development of new drugs.

scholarly journals Comparative Study of Antimicrobial, Anti-Inflammatory, and Antioxidant Activities of Different Parts from Pterocarpus Santalinoides l’Her. Ex. DC (Fabaceae)

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Aimé Cézaire Ayéna ◽  
Kokou Anani ◽  
Kossi Dosseh ◽  
Amegnona Agbonon ◽  
Messanvi Gbeassor
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Leaf Extracts ◽  
E Coli ◽  
Broth Microdilution Method ◽  
Anti Inflammatory ◽  
Pterocarpus Santalinoides

Aims. Pterocarpus santalinoides is used in Beninese folk medicine for treatment of gastroenteritis. This study aims to compare the antimicrobial, antioxidant, and anti-inflammatory activity of the hydroalcoholic extracts of the leaves, trunk bark, and root. Materials and Methods. The antimicrobial activity was evaluated by the broth microdilution method on 06 bacterial strains including 03 wild-type strains (Escherichia. coli 0157H, Salmonella sp., and Staphylococcus aureus sp.) and 03 reference strains (E. coli ATCC 25922, S. aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853), whereas the anti-inflammatory activity was performed by the carrageenan-induced paw edema method on rats. The DPPH-free radical scavenging was used to determine the antioxidant activity. Results. The MICs of the leaf extracts varied from 6.25 to 25 mg/mL for all strains. The MICs of the stem bark extracts were 6.5 to 25 μg/mL for five strains (E. coli 0157H, S. aureus ATCC 25922, Salmonella sp., E. coli ATCC 25922, and P. aeruginosa ATCC 27853) and 3.125 mg/mL for S. aureus. Concerning the root extracts, the MICs varied from 12.5 to 50 mg/mL. The best anti-inflammatory power was obtained with the stem bark extract with the percentages of inhibition of 36.09%, 38.98%, and 39.50%. The DPPH test showed that the hydroethanolic extract of the 03 parts of P. santalinoides has a moderate antiradical power compared to the control which was quercetin. Conclusion. In view of the different pharmacological activity recorded, the extract of the leaves should be recommended to treat patients suffering from gastroenteriditis.

scholarly journals Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

2019 ◽  
Vol 17 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Mert Ilhan ◽  
Fatma Tuğçe Gürağaç Dereli ◽  
Ibrahim Tümen ◽  
Esra Küpeli Akkol
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Scavenging Activity ◽  
Tail Flick Test ◽  
Etoac Extract ◽  
Anti Inflammatory

AbstractBryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. B. alba roots were extracted with n-hexane, ethyl acetate (EtOAc), and methanol, successively. The carrageenan-induced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. DPPH-radical-scavenging activity, ABTS radical–scavenging activity, total antioxidant activity, and hydroxyl radical– scavenging activity were assessed. Furthermore, the total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. Moreover, it had high phenolic and flavonoid contents. Therefore, B. alba roots could be alternative treatment method for rheumatic disorders.

scholarly journals Antioxidative and Potentially Anti-inflammatory Activity of Phenolics from Lovage Leaves Levisticum officinale Koch Elicited with Jasmonic Acid and Yeast Extract

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1441 ◽  
Author(s):  
Urszula Złotek ◽  
Urszula Szymanowska ◽  
Łukasz Pecio ◽  
Solomiia Kozachok ◽  
Anna Jakubczyk
Keyword(s):  
Phenolic Compounds ◽  
Jasmonic Acid ◽  
Yeast Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Anti Inflammatory ◽  
Main Component ◽  
Inhibition Ability

The effect of elicitation with jasmonic acids (JA) and yeast extract (YE) on the production of phenolic compounds as well as the antioxidant and anti-inflammatory properties of phenolic extracts of lovage was evaluated. The analysis of phenolic compounds carried out with the UPLC-MS technique indicated that rutin was the dominant flavonoid, while 5-caffeoylquinic acid was the main component in the phenolic acid fraction in the lovage leaves. The application of 10 µM JA increased the content of most of the identified phenolic compounds. The highest antioxidant activities estimated as free radical scavenging activity against ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and reducing power were determined for the sample elicited with 10 µM JA, while this value determined as iron chelating ability was the highest for the 0.1% YE-elicited lovage. The 0.1% and 1% YE elicitation also caused significant elevation of the lipoxygenase (LOX) inhibition ability, while all the concentrations of the tested elicitors significantly improved the ability to inhibit cyclooxygenase 2 (COX2) (best results were detected for the 10 µM JA and 0.1% YE2 sample). Thus, 0.1% yeast extract and 10 µM jasmonic acid proved to be most effective in elevation of the biological activity of lovage.

scholarly journals Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 314
Author(s):  
Jed Jebali ◽  
Hanene Ghazghazi ◽  
Chedia Aouadhi ◽  
Ines ELBini-Dhouib ◽  
Ridha Ben Salem ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Mentha Pulegium ◽  
Leaf Extracts ◽  
Traditional Remedies

Mint species (Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC50 value of 16.31 μg/mL and 570.08 μmol Fe2+/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.

scholarly journals Estimation of Cholinesterase Inhibitory and Antioxidant Effects of the Leaf Extracts of Anatolian Ficus carica var. domestica and their Total Phenol and Flavonoid Contents

2011 ◽  
Vol 6 (3) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Ilkay Erdogan Orhan ◽  
Osman Üstün ◽  
Bilge Şener
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenol ◽  
Ficus Carica ◽  
Cholinesterase Inhibition ◽  
Leaf Extracts ◽  
Antioxidant Power

Ficus carica var. domestica Tsch. & Rav. (common fig) is widely grown in Turkey and exported for its edible fruits. In this study, the n-hexane, chloroform, acetone, methanol, n-butanol, and water extracts of the leaves of F. carica var. domestica were screened for their cholinesterase inhibitory and antioxidant activities. Cholinesterase inhibition against acetyl- (AChE) and butyrylcholinesterase (BChE) was measured by the spectrophotometric method of Ellman at concentrations of 25, 50, and 100 μg/mL., while antioxidant activity was tested using three in vitro methods; 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, metal-chelation capacity, and ferric-reducing antioxidant power (FRAP). Total phenol and flavonoid contents of the extracts were determined spectrophotometrically. Our results revealed that the n-hexane and acetone extracts exerted a notable inhibition against both AChE (62.9±0.9% and 50.8±2.1%, respectively) and BChE (76.9±2.2% and 45.6±1.3%, respectively). However, they had low activity in the antioxidant tests. The chloroform extract was found to be the richest in total flavonoid content (252.5±1.1 mg/g quercetin equivalent), while the n-butanol extract had the highest total phenol amount (85.9±3.2 mg/g extract gallic acid equivalent).

scholarly journals In vitro antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum

2019 ◽  
Vol 19 (3) ◽  
pp. 100-108
Author(s):  
K. Thanzami ◽  
B.B. Kakoti ◽  
C. Lalremruati
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
In Vitro Models ◽  
Percent Inhibition ◽  
Anti Inflammatory

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum by in vitro models. Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation assays. The phenolic and flavonoid contents of the extract were also assessed. The extract at different concentrations showed significant percent inhibition of DPPH and lipid peroxidation and high reducing power when compared with the standard. The extract also showed a significantly high content of phenolics and flavonoids. Anti-inflammatory activity was studied by inhibition of albumin denaturation and human red blood cell (HRBC) membrane stabilization methods. The extract at different concentrations showed significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. Thus, the result indicates that the chloroform extract exhibited significant potential on anti-inflammatory and antioxidant activities.

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