Isokinetic Probe Total Water Content Measurements in the NASA Icing Research Tunnel with Supercooled Large Drop Conditions

In introduction studies, it is of great importance to determine how favorable the water balance of the studied species is under given environmental conditions. The aim of the research was to study the water regime of some varieties of chrysanthemums in the conditions of the Southern Ural. The study was conducted in 20182020 on the basis of the South-Ural Botanical Garden-Institute of the Ufa Federal Research Centre of Russian Academy of Sciences. The objects of the study were 23 varieties of Chrysanthemum hortorum Bailey. In the course of the research, the total water content, water-holding capacity, the content of mobile moisture, water deficit, and sublethal water deficit were determined. The analysis of water regime indicators is based on the method of artificial wilting (V.N. Tarenkov, L.N. Ivanova) and the method of saturation of plant samples (V.P. Moiseev, N.P. Reshetsky). Sublethal water deficit was determined by the method of T.K. Goryshina, L.I. Samsonova, modified by N.I. Bobrovskaya. The calculations were carried out by standard methods using statistical packages of the Microsoft Excel 2003 and the Agros 2.13 program. The studies made it possible to determine the value of the sublethal water deficit (28,4%) for the varieties of chrysanthemums in the conditions of the Bashkir Ural. It was found that the studied varieties during the growing season did not experience such a moisture deficit in the tissues that could lead to irreversible damage to the assimilating organs. Our experiments showed that chrysanthemum varieties in the Bashkir Cis-Ural under the same soil-climatic and agrotechnical conditions had the following range of indicators of total water content 70,090,4% and water-holding capacity 19,0064,6%. The analysis of variance revealed significant differences between water-holding capacity and the content of mobile moisture by varieties, the share of influence was 27,8531,71%. As a result of the correlation-regression analysis, the authors revealed a direct dependence of the indicators of mobile moisture content on the total water content, and an inverse one on the indicators of the content of mobile moisture and water-holding capacity.

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Determination of total water content in inulin using the volumetric Karl Fischer titration

Talanta ◽

10.1016/j.talanta.2006.02.024 ◽

2006 ◽

Vol 70 (5) ◽

pp. 1006-1010 ◽

Cited By ~ 19

Author(s):

Sébastien N. Ronkart ◽

Michel Paquot ◽

Christian Fougnies ◽

Claude Deroanne ◽

Jean-Claude Van Herck ◽

...

Keyword(s):

Water Content ◽

Total Water Content ◽

Total Water ◽

Karl Fischer Titration ◽

Karl Fischer

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Extra- and intracellular water spaces in muscles of man at rest and with dynamic exercise

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1982.243.3.r271 ◽

1982 ◽

Vol 243 (3) ◽

pp. R271-R280 ◽

Cited By ~ 39

Author(s):

G. Sjogaard ◽

B. Saltin

Keyword(s):

Water Content ◽

Vastus Lateralis ◽

Lactate Concentration ◽

Total Water Content ◽

Total Water ◽

Triceps Brachii ◽

Bicycle Exercise ◽

Tissue Samples ◽

Intracellular Space ◽

Extracellular Lactate

A method was established to analyze the extracellular water space (H2Oe) in small muscle tissue samples as [3H]inulin distribution space. After initial experiments on rats, the method was applied on 13 men and 6 women. Muscles with different fiber compositions (soleus, S; vastus lateralis, (VL; gastrocnemius, G; triceps brachii, TB) were studied at rest. The total water content was the same for all muscles, 320 (313-330) ml/100 g dry wt. However, differences were demonstrated for H2Oe, with 26-34 ml/100 g dry wt in VL and 38-54 ml/100 g dry wt in S, (P less than 0.05); the values for G and TB were in between those for VL and S. The differences in H2Oe were not related to the fiber composition of the muscles. During 3 x 3 min of intense bicycle exercise demanding about 120% VO2 max (6 men), total water content increased in VL from 313 to 359 ml/100 g dry wt and H2Oe increased from 34 to 60 ml/100 g dry wt (P less than 0.05), In TB, which is relatively inactive during bicycle exercise, no such changes occurred. The calculated intracellular lactate concentration increased in VL from 5.7 to 30.6 mmol/l H2Oi. The extracellular lactate concentration amounted to 13.6 mmol/l H2Oe at the end of exercise. The concentration gradient for lactate of 2 from intra- to extracellular space favored a flux of water to the intracellular space. The relative large increase in H2Oe may then be caused by a hydrostatic rather than an osmotic factor.U

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THE EFFECTS OF OESTRADIOL ON THE SKELETAL MUSCLE AND LIVER OF THE RAT

Journal of Endocrinology ◽

10.1677/joe.0.0080179 ◽

1952 ◽

Vol 8 (2) ◽

pp. 179-186

Author(s):

D. F. COLE

Keyword(s):

Skeletal Muscle ◽

Chemical Composition ◽

Nucleic Acid ◽

Water Content ◽

Total Water Content ◽

Total Nucleic Acid ◽

Total Water ◽

Intracellular Potassium ◽

Soluble Phosphorus ◽

Cell Electrolytes

The chemical composition of the liver and skeletal muscle in rats which had been treated with 1·0 μg. oestradiol has been investigated. The water and electrolyte content, creatine and creatinine phosphorus fractions and total nucleoproteins were studied. The changes in the water content are reciprocal to those already described for the uterus. The creatine and creatinine content and the total nucleic acid do not change significantly. There is an increase of the acid-soluble phosphorus in the first phase of the water increase in skeletal muscle which is related to some of the changes of intracellular potassium. In the liver there is an increase of the total water content between 28 and 36 hr. after injection, which is also associated with changes of acid-soluble phosphorus and cell electrolytes.

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The effects of hypothermia and insulin on the distribution of glucose in some tissues of the rat

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y68-060 ◽

1968 ◽

Vol 46 (3) ◽

pp. 399-405

Author(s):

Margaret J. Henderson ◽

Ann L. Haag ◽

R. E. Haist

Keyword(s):

Body Weight ◽

Water Content ◽

Rectus Abdominis ◽

Total Water Content ◽

Rectus Abdominis Muscle ◽

Glucose Load ◽

Total Water ◽

Muscle Tissues ◽

Intracellular Metabolism ◽

Intracellular Glucose

In fasted hypothermic rats (25 °C), after a glucose load of 1 g/kg body weight, in either the presence or absence of injected insulin (0.5 units/kg), the rate of disappearance of glucose from the blood was decreased as compared with that of normothermic rats (37 °C). Hypothermia did not change the total water content of diaphragm, rectus abdominis muscle, heart, or liver of these rats, nor did it change the sodium space of these tissues. Insulin-injected hypothermic rats did show an increase in the sodium space of the diaphragm. The glucose space of the muscle tissues was not changed by hypothermia, but was greatly increased by insulin in both hypothermic and normothermic rats. The glucose space of the liver was increased by insulin in normothermic rats, but in hypothermic rats an increase was found only when larger quantitites of insulin were injected. Intracellular glucose could not be detected in the diaphragm, rectus abdominis muscle, or heart of either normothermic or hypothermic rats in the absence of injected insulin, but was present in the liver of both. The injection of insulin caused intracellular glucose to accumulate in the diaphragm and heart of both normothermic and hypothermic rats. The level was increased by hypothermia only in the heart, suggesting that in this tissue the intracellular metabolism of glucose was decreased.

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