Isolation and Characterization of Extracellular Enzyme (Glutaminase and Urease) producing Bacteria isolated from Soil Samples of Different Regions of Gwalior, Madhya Pradesh, India
Microbial glutaminase and urease have demonstrated their benefits in various fields like medicinal, pharmaceutical and biotechnological industries. Keeping this viewpoint, the aim of the present study was the isolation and characterization of extracellular enzyme-producing bacteria from soil samples collected from different regions of Gwalior (M.P.). The isolated bacterial cultures were processed by serial dilution method and maintained on nutrient agar medium following standard microbiological laboratory practices for maintenance and preservation of bacteria. We screened out three enzyme producing strains of Salmonella sp., Proteus vulgaris and Bacillus subtilis. The screening was based on biochemical testing and enzyme assays. To accomplish this work, we used differential as well as selective media. All the selected isolates were able to produce enzymes like L-Glutaminase and Urease with different specific enzymatic activity. These bacterial isolates were not reported to show any type of allergenicity when their sequences were checked by bioinformatics tool Algpred. So, these bacterial isolates can be considered as an alternative source for the production of enzymes and can be used for largescale production of enzymes at the industrial level.