Isolation and characterization of bacteria from the rhizosphere and bulk soil of Stellera chamaejasme L.

This study is the first to describe the composition and characteristics of culturable bacterial isolates from the rhizosphere and bulk soil of the medicinal plant Stellera chamaejasme L. at different growth stages. Using a cultivation-dependent approach, a total of 148 isolates showing different phenotypic properties were obtained from the rhizosphere and bulk soil. Firmicutes and Actinobacteria were the major bacterial groups in both the rhizosphere and bulk soil at all 4 growth stages of S. chamaejasme. The diversity of the bacterial community in the rhizosphere was higher than that in bulk soil in flowering and fruiting stages. The abundance of bacterial communities in the rhizosphere changed with the growth stages and had a major shift at the fruiting stage. Dynamic changes of bacterial abundance and many bacterial groups in the rhizosphere were similar to those in bulk soil. Furthermore, most bacterial isolates exhibited single or multiple biochemical activities associated with S. chamaejasme growth, which revealed that bacteria with multiple physiological functions were abundant and widespread in the rhizosphere and bulk soil. These results are essential (i) for understanding the ecological roles of bacteria in the rhizosphere and bulk soil and (ii) as a foundation for further evaluating their efficacy as effective S. chamaejasme growth-promoting rhizobacteria.

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Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

Aceh International Journal of Science and Technology ◽

10.13170/aijst.4.3.3016 ◽

2015 ◽

Vol 4 (3) ◽

Author(s):

Kesi Kurnia ◽

Nina Hermayani Sadi ◽

Syafitri Jumianto

Keyword(s):

Heterotrophic Bacteria ◽

Water Environment ◽

Resistant Bacteria ◽

Bacterial Isolates ◽

Gram Negative ◽

Isolation And Characterization ◽

Agar Media ◽

Physical And Chemical ◽

Standard Disk

Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. This study aim to isolate and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, West Java, Indonesia. The water samples were collected along three points around Cilalay Lake. Water physical and chemical determination was performed using the Water Quality Checker. The bacterial isolates were screened on Triptone Glucose Yeast (TGY) agar plates. Afterwards selected isolates were grown on Nutrient Agar media 50% with supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates were resistant. The most abundant type of resistant bacteria to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water

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Isolation and Characterization of Thermophilic Bacteria Indigenous to Al-Ahsa Desert

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.3.56 ◽

2020 ◽

Vol 14 (3) ◽

pp. 2157-2163

Author(s):

Ahmed Anwar Al-Mulla ◽

Ashraf Khalifa

Keyword(s):

Standard Method ◽

Thermophilic Bacteria ◽

Soil Samples ◽

Biotechnological Applications ◽

Bacterial Isolates ◽

Phenotypic Characteristics ◽

Thermostable Enzymes ◽

Biological Resources ◽

Isolation And Characterization

Deciphering the biological resources across the Saudi niches is highly recommended for the prosperity. To this end, the aim of the current work was to isolate thermophilic bacteria from unexplored areas of Al-Ahsa region, and investigate their phenotypic characteristics. Three soil samples were collected from different desert sites of Al-Ahsa region. Thermophilic bacteria were isolated directly for soil samples into Thermus medium broth as a standard method. Single colonies of the actively growing bacterial isolates were preserved in 20% glycerol then kept at -80°C. The isolates were screened for production of thermostable enzymes using the commercially available kit API20E strip (bioMerieux, Marcy l’Etoile, France). Incubation were carried out at 50°C. It can be concluded that thermophilic bacteria in Al-Ahsa region harbor novel thermostable enzymes that might have biotechnological applications, in future.

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Isolation and Characterization of Extracellular Enzyme (Glutaminase and Urease) producing Bacteria isolated from Soil Samples of Different Regions of Gwalior, Madhya Pradesh, India

Research Journal of Biotechnology ◽

10.25303/167rjbt12221 ◽

2021 ◽

Vol 16 (7) ◽

pp. 122-129

Author(s):

Neha Sharma ◽

Shuchi Kaushik ◽

Rajesh Singh Tomar

Keyword(s):

Extracellular Enzyme ◽

Soil Samples ◽

Dilution Method ◽

Bacterial Isolates ◽

Alternative Source ◽

Isolation And Characterization ◽

Microbiological Laboratory ◽

Biochemical Testing ◽

Industrial Level

Microbial glutaminase and urease have demonstrated their benefits in various fields like medicinal, pharmaceutical and biotechnological industries. Keeping this viewpoint, the aim of the present study was the isolation and characterization of extracellular enzyme-producing bacteria from soil samples collected from different regions of Gwalior (M.P.). The isolated bacterial cultures were processed by serial dilution method and maintained on nutrient agar medium following standard microbiological laboratory practices for maintenance and preservation of bacteria. We screened out three enzyme producing strains of Salmonella sp., Proteus vulgaris and Bacillus subtilis. The screening was based on biochemical testing and enzyme assays. To accomplish this work, we used differential as well as selective media. All the selected isolates were able to produce enzymes like L-Glutaminase and Urease with different specific enzymatic activity. These bacterial isolates were not reported to show any type of allergenicity when their sequences were checked by bioinformatics tool Algpred. So, these bacterial isolates can be considered as an alternative source for the production of enzymes and can be used for largescale production of enzymes at the industrial level.

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Isolation and Characterization of Bacterial Isolates Algicidal against a Harmful Bloom-forming Cyanobacterium Microcystis aeruginosa

Biocontrol Science ◽

10.4265/bio.17.107 ◽

2012 ◽

Vol 17 (3) ◽

pp. 107-114 ◽

Cited By ~ 4

Author(s):

YANG LI ◽

WEI HONGYI ◽

MASAHARU KOMATSU ◽

KENICHI ISHIBASHI ◽

LIN JINSAN ◽

...

Keyword(s):

Microcystis Aeruginosa ◽

Bacterial Isolates ◽

Isolation And Characterization

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Isolation and characterization of bacteria from rusted iron materials

Bangladesh Journal of Botany ◽

10.3329/bjb.v39i2.7479 ◽

1970 ◽

Vol 39 (2) ◽

pp. 185-191 ◽

Cited By ~ 5

Author(s):

Muhbubar Rahman Khan ◽

Mihir Lal Saha ◽

Nahmina Begum ◽

Mohammad Nurul Islam ◽

Sirajul Hoque

Keyword(s):

Ferrous Sulfate ◽

Bacterial Isolates ◽

Primary Screening ◽

Isolation And Characterization ◽

Iron Salts ◽

Sulfur Oxidizing ◽

Good Number ◽

Number Of Bacteria ◽

Ammonium Ferric Citrate

Seventy bacterial isolates were recovered from different rusted iron materials using modified NP glucose, 9K and sulfur oxidizing media. Thirty four isolates were selected after primary screening on the basis of their growth in modified NP glucose medium. Out of 34 isolates 5 belonged to the genus Kurthia, 6 Thiobacillus, 16 Bacillus and remaining 7 were Acidiphilium, Oscillospira, Sulfobacillus, Alicyclobacillus, Neisseria, Pseudomonas and Rahnella. One of the isolated organisms, Bacillus megaterium (F16/2), was found to be thermophilic. The organisms showed better growth in presence of iron salts like ferrous sulfate, ferric sulfate and ammonium ferric citrate. Five isolates were found to transform ferrous (Fe2+) to ferric (Fe3+) of which Bacillus subtilis (F8) was most efficient. A good number of bacteria were associated with oxidation of iron. Key words: Isolation; Bacteria; Rusted iron; Transformation DOI: 10.3329/bjb.v39i2.7479 Bangladesh J. Bot. 39(2): 185-191, 2010 (December)

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Isolation and characterization of n-butane-utilizing microorganisms

Canadian Journal of Microbiology ◽

10.1139/m72-186 ◽

1972 ◽

Vol 18 (8) ◽

pp. 1191-1195 ◽

Cited By ~ 30

Author(s):

A. G. McLee ◽

Agnes C. Kormendy ◽

M. Wayman

Keyword(s):

Partial Pressure ◽

Growth Rates ◽

Bacterial Isolates ◽

Partial Pressure Of Oxygen ◽

Bacterial Strains ◽

Isolation And Characterization ◽

Partial Pressures ◽

High Partial Pressure

Fifteen bacterial strains and four molds capable of growth on n-butane were isolated and partially classified. The bacteria were mostly Arthrobacter sp. and Brevibacterium sp.; among the molds, Penicillium nigricans, Allescheria boydii, and Graphium cumeiferum were identified, while the remaining mold had the appearance of Gliocladium, but was not firmly identified. Although able to grow on other alkanes and orthodox media, the bacterial isolates could not use methane. Growth rates on n-butane were unaffected by varying air or substrate partial pressures in the range of 10–90% atmosphere. High partial pressure of oxygen was inhibitory to most bacterial isolates, the degree of inhibition varying widely, however. Growth rates on n-butanol and on glucose were significantly higher than those on n-butane. Among the molds, only the Graphium would grow well in submerged, shaking culture.

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Meat starter cultures: Isolation and characterization of lactic acid bacteria from traditional sausages

Acta Universitatis Sapientiae Alimentaria ◽

10.2478/ausal-2019-0004 ◽

2019 ◽

Vol 12 (1) ◽

pp. 54-69 ◽

Cited By ~ 2

Author(s):

É. Laslo ◽

É. György ◽

A. Czikó

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Raw Materials ◽

Meat Products ◽

Starter Cultures ◽

Probiotic Properties ◽

Meat Industry ◽

Bacterial Isolates ◽

Isolation And Characterization

Abstract Fermented meat products represent an important segment of our alimentation. Obtaining these products is based on beneficial microorganism activity. In the case of traditional food products, these are commercial starters or autochthonous microflora. Fermentation of raw materials is mainly done by sugar metabolization of lactic acid bacteria (LAB). In addition, these microorganisms can have other beneficial properties too such as probiotic properties, antimicrobial compound production abilities, etc. In order to meet consumer demands, starter cultures are continuously developed to produce high-quality, healthy, and tasty products, thus contributing to guaranteeing microbiological safety and to improving one or more sensory characteristics, technological, nutritional, or health properties of the fermented products. The aim of our research is to determine the technological properties of autochthonous lactic acid bacteria originated from commercial fresh sausages in order to select and use them as potential starter cultures in the meat industry. In our work, we determined the relevant characteristics (such as salt tolerance, proteolytic activity, antimicrobial activity, and antibiotic resistance) of bacteria isolated from 16 fresh sausages. Based on our results, the studied bacterial isolates originated from sausages could be potentially used as autochthonous meat starter cultures.

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Isolation and Characterization of Chlorpyrifos Degrading Bacterial Isolates from Rice Soil

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2017.609.042 ◽

2017 ◽

Vol 6 (9) ◽

pp. 329-340

Author(s):

Sonali Priyadarshini Tushar Kanti Dangar

Keyword(s):

Rice Soil ◽

Bacterial Isolates ◽

Isolation And Characterization

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Isolation and Characterization of Urease-Producing Soil Bacteria

International Journal of Microbiology ◽

10.1155/2021/8888641 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Eshetu Mekonnen ◽

Ameha Kebede ◽

Asefa Nigussie ◽

Gessese Kebede ◽

Mesfin Tafesse

Keyword(s):

Gene Sequence ◽

Growth Conditions ◽

Rrna Gene ◽

Bacterial Isolates ◽

Isolation And Characterization ◽

Urease Production ◽

Profile Characteristics ◽

Geological Formations ◽

Ethiopian Soils

Urease is an enzyme produced by ureolytic microorganisms which hydrolyzes urea into ammonia and carbon dioxide. Microbial urease has wide applications in biotechnology, agriculture, medicine, construction, and geotechnical engineering. Urease-producing microbes can be isolated from different ecosystems such as soil, oceans, and various geological formations. The aim of this study was to isolate and characterize rapid urease-producing bacteria from Ethiopian soils. Using qualitative urease activity assay, twenty urease-producing bacterial isolates were screened and selected. Among these, three expressed urease at high rates as determined by a conductivity assay. The isolates were further characterized with respect to their biochemical, morphological, molecular, and exoenzyme profile characteristics. The active urease-producing bacterial isolates were found to be nonhalophilic to slightly halophilic neutrophiles and aerobic mesophiles with a range of tolerance towards pH (4.0–10.0), NaCl (0.25—5%), and temperature (20–40°C). According to the API ZYM assays, all three isolates were positive for alkaline phosphatase, leucine aryl amidase, acid phosphatase, and naphthol_AS_BI_phosphohydrolase. The closest described relatives of the selected three isolates (Isolate_3, Isolate_7, and Isolate_11) were Bacillus paramycoides, Citrobacter sedlakii, and Enterobacter bugandensis with 16S rRNA gene sequence identity of 99.0, 99.2, and 98.9%, respectively. From the study, it was concluded that the three strains appear to have a relatively higher potential for urease production and be able to grow under a wider range of growth conditions.

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